Surfactant-free, biodegradable nanoparticles for aerosol therapy based on the branched polyesters, DEAPA-PVAL-g-PLGA
This study describes the development of surfactant-free, biodegradable nanoparticle systems with varying physicochemical properties and their suitability for pulmonary application via nebulization. Nanoparticle suspensions were formulated from the branched polyester, diethylaminopropyl amine-poly(vi...
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| Veröffentlicht in: | Pharmaceutical research 2003-12, Vol.20 (12), p.2011-2020 |
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| creator | DAILEY, L. A KLEEMANN, E WITTMAR, M GESSLER, T SCHMEHL, T ROBERTS, C SEEGER, W KISSEL, T |
| description | This study describes the development of surfactant-free, biodegradable nanoparticle systems with varying physicochemical properties and their suitability for pulmonary application via nebulization.
Nanoparticle suspensions were formulated from the branched polyester, diethylaminopropyl amine-poly(vinyl alcohol)-grafted-poly(lactide-co-glycolide) (DEAPA-PVAL-g-PLGA) alone, as well as with increasing amounts of carboxymethyl cellulose (CMC). Particle size, zeta potential, turbidity, and morphology (atomic force microscopy) were characterized. Three formulations were chosen for further study: Cationic nanoparticles without CMC, cationic nanoparticles with CMC, and anionic nanoparticles with an excess of CMC. Nanoparticle degradation was characterized, as well as stability during nebulization. Nanoparticle-cell interactions were investigated and quantified using confocal laser scanning microscopy and fluorescence spectrometry.
Nanoparticles ranged in size from 70-250 nm and displayed zeta potentials of +58.9 to -46.6 mV. Anionic nanoparticles showed the highest stability during nebulization. The degradation rate of each nanoparticle formulation decreased with increasing amounts of CMC. Cell association was highest among cationic nanoparticles (57% and 30%, respectively), although these were not internalized. Despite a lower rate of cell association (3%), anionic nanoparticles were internalized by A549 cells.
Surfactant-free nanoparticles from DEAPA-PVAL-g-PLGA are versatile drug delivery systems; however, only the anionic formulations investigated were proven suitable for aerosol therapy. |
| doi_str_mv | 10.1023/B:PHAM.0000008051.94834.10 |
| format | Article |
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Nanoparticle suspensions were formulated from the branched polyester, diethylaminopropyl amine-poly(vinyl alcohol)-grafted-poly(lactide-co-glycolide) (DEAPA-PVAL-g-PLGA) alone, as well as with increasing amounts of carboxymethyl cellulose (CMC). Particle size, zeta potential, turbidity, and morphology (atomic force microscopy) were characterized. Three formulations were chosen for further study: Cationic nanoparticles without CMC, cationic nanoparticles with CMC, and anionic nanoparticles with an excess of CMC. Nanoparticle degradation was characterized, as well as stability during nebulization. Nanoparticle-cell interactions were investigated and quantified using confocal laser scanning microscopy and fluorescence spectrometry.
Nanoparticles ranged in size from 70-250 nm and displayed zeta potentials of +58.9 to -46.6 mV. Anionic nanoparticles showed the highest stability during nebulization. The degradation rate of each nanoparticle formulation decreased with increasing amounts of CMC. Cell association was highest among cationic nanoparticles (57% and 30%, respectively), although these were not internalized. Despite a lower rate of cell association (3%), anionic nanoparticles were internalized by A549 cells.
Surfactant-free nanoparticles from DEAPA-PVAL-g-PLGA are versatile drug delivery systems; however, only the anionic formulations investigated were proven suitable for aerosol therapy.</description><identifier>ISSN: 0724-8741</identifier><identifier>EISSN: 1573-904X</identifier><identifier>DOI: 10.1023/B:PHAM.0000008051.94834.10</identifier><identifier>PMID: 14725368</identifier><identifier>CODEN: PHREEB</identifier><language>eng</language><publisher>New York, NY: Springer</publisher><subject>Aerosols ; Alcohol ; Biodegradation ; Biological and medical sciences ; Biotransformation ; Carboxymethylcellulose Sodium ; Cellulose ; Chemistry, Pharmaceutical ; Drug Delivery Systems ; Drug Stability ; General pharmacology ; Lactic Acid - chemistry ; Medical sciences ; Microscopy ; Microscopy, Atomic Force ; Microscopy, Confocal ; Microspheres ; Nanoparticles ; Nephelometry and Turbidimetry ; Particle Size ; Pharmaceutic Aids ; Pharmaceutical technology. Pharmaceutical industry ; Pharmacology. Drug treatments ; Polyesters - chemistry ; Polyglactin 910 - analogs & derivatives ; Polyglactin 910 - chemistry ; Polyglycolic Acid - chemistry ; Polylactic Acid-Polyglycolic Acid Copolymer ; Polymers - chemistry ; Polyvinyl Alcohol - chemistry ; Scientific imaging ; Spectrometry, Fluorescence ; Surfactants ; Suspensions ; Toxicity</subject><ispartof>Pharmaceutical research, 2003-12, Vol.20 (12), p.2011-2020</ispartof><rights>2004 INIST-CNRS</rights><rights>Copyright Kluwer Academic Publishers Dec 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c429t-22d864897c7071b0fa8dd4385e9d6a310ff234703a1613c7cdfa27f07ba3b8ff3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15381627$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14725368$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>DAILEY, L. A</creatorcontrib><creatorcontrib>KLEEMANN, E</creatorcontrib><creatorcontrib>WITTMAR, M</creatorcontrib><creatorcontrib>GESSLER, T</creatorcontrib><creatorcontrib>SCHMEHL, T</creatorcontrib><creatorcontrib>ROBERTS, C</creatorcontrib><creatorcontrib>SEEGER, W</creatorcontrib><creatorcontrib>KISSEL, T</creatorcontrib><title>Surfactant-free, biodegradable nanoparticles for aerosol therapy based on the branched polyesters, DEAPA-PVAL-g-PLGA</title><title>Pharmaceutical research</title><addtitle>Pharm Res</addtitle><description>This study describes the development of surfactant-free, biodegradable nanoparticle systems with varying physicochemical properties and their suitability for pulmonary application via nebulization.
Nanoparticle suspensions were formulated from the branched polyester, diethylaminopropyl amine-poly(vinyl alcohol)-grafted-poly(lactide-co-glycolide) (DEAPA-PVAL-g-PLGA) alone, as well as with increasing amounts of carboxymethyl cellulose (CMC). Particle size, zeta potential, turbidity, and morphology (atomic force microscopy) were characterized. Three formulations were chosen for further study: Cationic nanoparticles without CMC, cationic nanoparticles with CMC, and anionic nanoparticles with an excess of CMC. Nanoparticle degradation was characterized, as well as stability during nebulization. Nanoparticle-cell interactions were investigated and quantified using confocal laser scanning microscopy and fluorescence spectrometry.
Nanoparticles ranged in size from 70-250 nm and displayed zeta potentials of +58.9 to -46.6 mV. Anionic nanoparticles showed the highest stability during nebulization. The degradation rate of each nanoparticle formulation decreased with increasing amounts of CMC. Cell association was highest among cationic nanoparticles (57% and 30%, respectively), although these were not internalized. Despite a lower rate of cell association (3%), anionic nanoparticles were internalized by A549 cells.
Surfactant-free nanoparticles from DEAPA-PVAL-g-PLGA are versatile drug delivery systems; however, only the anionic formulations investigated were proven suitable for aerosol therapy.</description><subject>Aerosols</subject><subject>Alcohol</subject><subject>Biodegradation</subject><subject>Biological and medical sciences</subject><subject>Biotransformation</subject><subject>Carboxymethylcellulose Sodium</subject><subject>Cellulose</subject><subject>Chemistry, Pharmaceutical</subject><subject>Drug Delivery Systems</subject><subject>Drug Stability</subject><subject>General pharmacology</subject><subject>Lactic Acid - chemistry</subject><subject>Medical sciences</subject><subject>Microscopy</subject><subject>Microscopy, Atomic Force</subject><subject>Microscopy, Confocal</subject><subject>Microspheres</subject><subject>Nanoparticles</subject><subject>Nephelometry and Turbidimetry</subject><subject>Particle Size</subject><subject>Pharmaceutic Aids</subject><subject>Pharmaceutical technology. Pharmaceutical industry</subject><subject>Pharmacology. Drug treatments</subject><subject>Polyesters - chemistry</subject><subject>Polyglactin 910 - analogs & derivatives</subject><subject>Polyglactin 910 - chemistry</subject><subject>Polyglycolic Acid - chemistry</subject><subject>Polylactic Acid-Polyglycolic Acid Copolymer</subject><subject>Polymers - chemistry</subject><subject>Polyvinyl Alcohol - chemistry</subject><subject>Scientific imaging</subject><subject>Spectrometry, Fluorescence</subject><subject>Surfactants</subject><subject>Suspensions</subject><subject>Toxicity</subject><issn>0724-8741</issn><issn>1573-904X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNpdkUtv1DAURi0EosPAX0BWJVg1g1-Jne7SUlqkQYzEQ-ysGz_aVJk42Mli_j1OO9JIeGPZ91z76jsInVOyoYTxT1eXu7vm24Y8LUVKuqmF4iIXX6AVLSUvaiL-vEQrIpkolBT0DL1J6XGhaS1eozMqJCt5pVZo-jFHD2aCYSp8dO4Ct12w7j6ChbZ3eIAhjBCnzvQuYR8iBhdDCj2eHlyE8YBbSM7iMCwXuI0wmId8HkN_cGlyMV3gzzfNril2v5ttcV_strfNW_TKQ5_cu-O-Rr--3Py8viu232-_XmfMCFZPBWNWVULV0kgiaUs8KGsFV6WrbQWcEu8ZF5JwoBXlRhrrgUlPZAu8Vd7zNfr4_O4Yw985j6P3XTKu72FwYU5a0pIwqqoMnv8HPoY5Dnk2zRirSkly7mt0-QyZHECKzusxdnuIB02JXsToK72I0Scx-klMLubm98cf5nbv7Kn1aCIDH44AJAO9X4Ls0okruaIVk_wf_qGWJw</recordid><startdate>20031201</startdate><enddate>20031201</enddate><creator>DAILEY, L. A</creator><creator>KLEEMANN, E</creator><creator>WITTMAR, M</creator><creator>GESSLER, T</creator><creator>SCHMEHL, T</creator><creator>ROBERTS, C</creator><creator>SEEGER, W</creator><creator>KISSEL, T</creator><general>Springer</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M1P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20031201</creationdate><title>Surfactant-free, biodegradable nanoparticles for aerosol therapy based on the branched polyesters, DEAPA-PVAL-g-PLGA</title><author>DAILEY, L. 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Pharmaceutical industry</topic><topic>Pharmacology. Drug treatments</topic><topic>Polyesters - chemistry</topic><topic>Polyglactin 910 - analogs & derivatives</topic><topic>Polyglactin 910 - chemistry</topic><topic>Polyglycolic Acid - chemistry</topic><topic>Polylactic Acid-Polyglycolic Acid Copolymer</topic><topic>Polymers - chemistry</topic><topic>Polyvinyl Alcohol - chemistry</topic><topic>Scientific imaging</topic><topic>Spectrometry, Fluorescence</topic><topic>Surfactants</topic><topic>Suspensions</topic><topic>Toxicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>DAILEY, L. A</creatorcontrib><creatorcontrib>KLEEMANN, E</creatorcontrib><creatorcontrib>WITTMAR, M</creatorcontrib><creatorcontrib>GESSLER, T</creatorcontrib><creatorcontrib>SCHMEHL, T</creatorcontrib><creatorcontrib>ROBERTS, C</creatorcontrib><creatorcontrib>SEEGER, W</creatorcontrib><creatorcontrib>KISSEL, T</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Pharmaceutical research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>DAILEY, L. A</au><au>KLEEMANN, E</au><au>WITTMAR, M</au><au>GESSLER, T</au><au>SCHMEHL, T</au><au>ROBERTS, C</au><au>SEEGER, W</au><au>KISSEL, T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Surfactant-free, biodegradable nanoparticles for aerosol therapy based on the branched polyesters, DEAPA-PVAL-g-PLGA</atitle><jtitle>Pharmaceutical research</jtitle><addtitle>Pharm Res</addtitle><date>2003-12-01</date><risdate>2003</risdate><volume>20</volume><issue>12</issue><spage>2011</spage><epage>2020</epage><pages>2011-2020</pages><issn>0724-8741</issn><eissn>1573-904X</eissn><coden>PHREEB</coden><abstract>This study describes the development of surfactant-free, biodegradable nanoparticle systems with varying physicochemical properties and their suitability for pulmonary application via nebulization.
Nanoparticle suspensions were formulated from the branched polyester, diethylaminopropyl amine-poly(vinyl alcohol)-grafted-poly(lactide-co-glycolide) (DEAPA-PVAL-g-PLGA) alone, as well as with increasing amounts of carboxymethyl cellulose (CMC). Particle size, zeta potential, turbidity, and morphology (atomic force microscopy) were characterized. Three formulations were chosen for further study: Cationic nanoparticles without CMC, cationic nanoparticles with CMC, and anionic nanoparticles with an excess of CMC. Nanoparticle degradation was characterized, as well as stability during nebulization. Nanoparticle-cell interactions were investigated and quantified using confocal laser scanning microscopy and fluorescence spectrometry.
Nanoparticles ranged in size from 70-250 nm and displayed zeta potentials of +58.9 to -46.6 mV. Anionic nanoparticles showed the highest stability during nebulization. The degradation rate of each nanoparticle formulation decreased with increasing amounts of CMC. Cell association was highest among cationic nanoparticles (57% and 30%, respectively), although these were not internalized. Despite a lower rate of cell association (3%), anionic nanoparticles were internalized by A549 cells.
Surfactant-free nanoparticles from DEAPA-PVAL-g-PLGA are versatile drug delivery systems; however, only the anionic formulations investigated were proven suitable for aerosol therapy.</abstract><cop>New York, NY</cop><pub>Springer</pub><pmid>14725368</pmid><doi>10.1023/B:PHAM.0000008051.94834.10</doi><tpages>10</tpages></addata></record> |
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| subjects | Aerosols Alcohol Biodegradation Biological and medical sciences Biotransformation Carboxymethylcellulose Sodium Cellulose Chemistry, Pharmaceutical Drug Delivery Systems Drug Stability General pharmacology Lactic Acid - chemistry Medical sciences Microscopy Microscopy, Atomic Force Microscopy, Confocal Microspheres Nanoparticles Nephelometry and Turbidimetry Particle Size Pharmaceutic Aids Pharmaceutical technology. Pharmaceutical industry Pharmacology. Drug treatments Polyesters - chemistry Polyglactin 910 - analogs & derivatives Polyglactin 910 - chemistry Polyglycolic Acid - chemistry Polylactic Acid-Polyglycolic Acid Copolymer Polymers - chemistry Polyvinyl Alcohol - chemistry Scientific imaging Spectrometry, Fluorescence Surfactants Suspensions Toxicity |
| title | Surfactant-free, biodegradable nanoparticles for aerosol therapy based on the branched polyesters, DEAPA-PVAL-g-PLGA |
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