Initiation and Characterization of Keratinocyte Cultures from Biopsies of Normal Human Conjunctiva

The purpose of the present study was to establish and characterize serum-free epithelial cultures of normal human conjunctiva using fresh biopsy tissue. To this end, small pieces of normal conjunctiva were biopsied from patients undergoing routine cataract surgery. Fragments of the tissue were place...

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Veröffentlicht in:	Experimental eye research 2002-01, Vol.74 (1), p.61-69
Hauptverfasser:	Risse Marsh, Barbara C., Massaro-Giordano, Mina, Marshall, Christine M., Lavker, Robert M., Jensen, Pamela J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biopsy cadherin Calcium - pharmacology Cell Culture Techniques - methods conjunctiva Conjunctiva - cytology Conjunctiva - drug effects Conjunctiva - metabolism cornea culture Culture Media, Serum-Free differentiation Dose-Response Relationship, Drug Epithelial Cells - cytology Epithelial Cells - drug effects Epithelial Cells - metabolism epithelium explant culture Eye and associated structures. Visual pathways and centers. Vision Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology human Humans in vitro involucrin keratin keratinocyte Keratinocytes - cytology Keratinocytes - drug effects Keratinocytes - metabolism Keratins - metabolism Vertebrates: nervous system and sense organs
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