Antioxidant protection in cultured corneal cells and whole corneas submitted to UV-B exposure
Several corneal pathologies are characterized by the presence of reactive oxygen species (ROS); therefore, we evaluated the protection afforded by pirenoxine and melatonin to corneal cell culture and whole rabbit cornea from ultraviolet exposure and other oxidant systems. Rabbit cornea cell (SIRC) p...
Gespeichert in:
| Veröffentlicht in: | Journal of photochemistry and photobiology. B, Biology Biology, 2003-10, Vol.71 (1), p.59-68 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 68 |
|---|---|
| container_issue | 1 |
| container_start_page | 59 |
| container_title | Journal of photochemistry and photobiology. B, Biology |
| container_volume | 71 |
| creator | Ciuffi, Mario Pisanello, Marcella Pagliai, Gabriella Raimondi, Laura Franchi-Micheli, Sergio Cantore, Miriam Mazzetti, Luca Failli, Paola |
| description | Several corneal pathologies are characterized by the presence of reactive oxygen species (ROS); therefore, we evaluated the protection afforded by pirenoxine and melatonin to corneal cell culture and whole rabbit cornea from ultraviolet exposure and other oxidant systems.
Rabbit cornea cell (SIRC) plates and whole corneas were exposed to UV-B (80 or 800 mJ/cm
2) or incubated with fMLP-stimulated autologous macrophages, in the presence or absence of pirenoxine or melatonin (10
−5 M). The protective activity of compounds was assessed by measuring superoxide anion formation, inhibition of oxidation and mitochondrial viability. Moreover the ex vivo protective effect of pirenoxine and melatonin was verified in the whole cornea submitted to UV-B exposure in vitro.
Our experimental data demonstrate that pirenoxine and melatonin were able to inhibit the superoxide formation and oxidative effect in cell culture and whole rabbit corneas submitted to UV-B exposure or to incubation with fMLP-stimulated autologous macrophages.
Mitochondrial viability was restored in epithelial cells of rabbit cornea but not in SIRCs.
Moreover, both compounds are also able to increase ex vivo epithelial corneal cell defences against the in vitro UV-B induced lipid peroxidation. |
| doi_str_mv | 10.1016/j.jphotobiol.2003.07.004 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_71498522</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1011134403001131</els_id><sourcerecordid>71498522</sourcerecordid><originalsourceid>FETCH-LOGICAL-c486t-cc5977505e9a09f535321d8ebde3dedc0224afe75f902023f14935dacd5bf8a73</originalsourceid><addsrcrecordid>eNqFkMtOBCEQRYnR-P4Fw8pdtwU0Q_dSja_ExI26M4SB6sikpxmB9vH3MplJXMqGonIvVfcQQhnUDNjsYlEvVu8hh7kPQ80BRA2qBmh2yCFrlaj4rOW7pQbGKiaa5oAcpbSAcuRM7ZMD1qhSNXBI3i7H7MO3d2bMdBVDRlveI_UjtdOQp4iO2hBHNAO1OAyJmtHRr_cw4LafaJrmS59zUeZAX16rK4rfq5CK94Ts9WZIeLq9j8nL7c3z9X31-HT3cH35WNmmneXKWtkpJUFiZ6DrpZCCM9fi3KFw6Cxw3pgelew74MBFz5pOSGesk_O-NUock_PNvyXBx4Qp66VP63XNiGFKWhVDKzkvwnYjtDGkFLHXq-iXJv5oBnqNVi_0H1q9RqtB6YK2WM-2M0pcdH_GLcsiuNoIsCT99Bh1sh5Hi87HQlW74P-f8gv5VZFm</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>71498522</pqid></control><display><type>article</type><title>Antioxidant protection in cultured corneal cells and whole corneas submitted to UV-B exposure</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Ciuffi, Mario ; Pisanello, Marcella ; Pagliai, Gabriella ; Raimondi, Laura ; Franchi-Micheli, Sergio ; Cantore, Miriam ; Mazzetti, Luca ; Failli, Paola</creator><creatorcontrib>Ciuffi, Mario ; Pisanello, Marcella ; Pagliai, Gabriella ; Raimondi, Laura ; Franchi-Micheli, Sergio ; Cantore, Miriam ; Mazzetti, Luca ; Failli, Paola</creatorcontrib><description>Several corneal pathologies are characterized by the presence of reactive oxygen species (ROS); therefore, we evaluated the protection afforded by pirenoxine and melatonin to corneal cell culture and whole rabbit cornea from ultraviolet exposure and other oxidant systems.
Rabbit cornea cell (SIRC) plates and whole corneas were exposed to UV-B (80 or 800 mJ/cm
2) or incubated with fMLP-stimulated autologous macrophages, in the presence or absence of pirenoxine or melatonin (10
−5 M). The protective activity of compounds was assessed by measuring superoxide anion formation, inhibition of oxidation and mitochondrial viability. Moreover the ex vivo protective effect of pirenoxine and melatonin was verified in the whole cornea submitted to UV-B exposure in vitro.
Our experimental data demonstrate that pirenoxine and melatonin were able to inhibit the superoxide formation and oxidative effect in cell culture and whole rabbit corneas submitted to UV-B exposure or to incubation with fMLP-stimulated autologous macrophages.
Mitochondrial viability was restored in epithelial cells of rabbit cornea but not in SIRCs.
Moreover, both compounds are also able to increase ex vivo epithelial corneal cell defences against the in vitro UV-B induced lipid peroxidation.</description><identifier>ISSN: 1011-1344</identifier><identifier>EISSN: 1873-2682</identifier><identifier>DOI: 10.1016/j.jphotobiol.2003.07.004</identifier><identifier>PMID: 14705640</identifier><language>eng</language><publisher>Switzerland: Elsevier B.V</publisher><subject>Alveolar macrophages ; Animals ; Antioxidants - pharmacology ; Cells, Cultured ; Cornea ; Cornea - cytology ; Cornea - drug effects ; Cornea - radiation effects ; Macrophages - physiology ; Melatonin ; Oxazines - pharmacology ; Pirenoxine ; Rabbits ; Reactive Oxygen Species ; Spectrometry, Fluorescence ; Ultraviolet Rays ; UV-B</subject><ispartof>Journal of photochemistry and photobiology. B, Biology, 2003-10, Vol.71 (1), p.59-68</ispartof><rights>2003 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c486t-cc5977505e9a09f535321d8ebde3dedc0224afe75f902023f14935dacd5bf8a73</citedby><cites>FETCH-LOGICAL-c486t-cc5977505e9a09f535321d8ebde3dedc0224afe75f902023f14935dacd5bf8a73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jphotobiol.2003.07.004$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,782,786,3554,27933,27934,46004</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14705640$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ciuffi, Mario</creatorcontrib><creatorcontrib>Pisanello, Marcella</creatorcontrib><creatorcontrib>Pagliai, Gabriella</creatorcontrib><creatorcontrib>Raimondi, Laura</creatorcontrib><creatorcontrib>Franchi-Micheli, Sergio</creatorcontrib><creatorcontrib>Cantore, Miriam</creatorcontrib><creatorcontrib>Mazzetti, Luca</creatorcontrib><creatorcontrib>Failli, Paola</creatorcontrib><title>Antioxidant protection in cultured corneal cells and whole corneas submitted to UV-B exposure</title><title>Journal of photochemistry and photobiology. B, Biology</title><addtitle>J Photochem Photobiol B</addtitle><description>Several corneal pathologies are characterized by the presence of reactive oxygen species (ROS); therefore, we evaluated the protection afforded by pirenoxine and melatonin to corneal cell culture and whole rabbit cornea from ultraviolet exposure and other oxidant systems.
Rabbit cornea cell (SIRC) plates and whole corneas were exposed to UV-B (80 or 800 mJ/cm
2) or incubated with fMLP-stimulated autologous macrophages, in the presence or absence of pirenoxine or melatonin (10
−5 M). The protective activity of compounds was assessed by measuring superoxide anion formation, inhibition of oxidation and mitochondrial viability. Moreover the ex vivo protective effect of pirenoxine and melatonin was verified in the whole cornea submitted to UV-B exposure in vitro.
Our experimental data demonstrate that pirenoxine and melatonin were able to inhibit the superoxide formation and oxidative effect in cell culture and whole rabbit corneas submitted to UV-B exposure or to incubation with fMLP-stimulated autologous macrophages.
Mitochondrial viability was restored in epithelial cells of rabbit cornea but not in SIRCs.
Moreover, both compounds are also able to increase ex vivo epithelial corneal cell defences against the in vitro UV-B induced lipid peroxidation.</description><subject>Alveolar macrophages</subject><subject>Animals</subject><subject>Antioxidants - pharmacology</subject><subject>Cells, Cultured</subject><subject>Cornea</subject><subject>Cornea - cytology</subject><subject>Cornea - drug effects</subject><subject>Cornea - radiation effects</subject><subject>Macrophages - physiology</subject><subject>Melatonin</subject><subject>Oxazines - pharmacology</subject><subject>Pirenoxine</subject><subject>Rabbits</subject><subject>Reactive Oxygen Species</subject><subject>Spectrometry, Fluorescence</subject><subject>Ultraviolet Rays</subject><subject>UV-B</subject><issn>1011-1344</issn><issn>1873-2682</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtOBCEQRYnR-P4Fw8pdtwU0Q_dSja_ExI26M4SB6sikpxmB9vH3MplJXMqGonIvVfcQQhnUDNjsYlEvVu8hh7kPQ80BRA2qBmh2yCFrlaj4rOW7pQbGKiaa5oAcpbSAcuRM7ZMD1qhSNXBI3i7H7MO3d2bMdBVDRlveI_UjtdOQp4iO2hBHNAO1OAyJmtHRr_cw4LafaJrmS59zUeZAX16rK4rfq5CK94Ts9WZIeLq9j8nL7c3z9X31-HT3cH35WNmmneXKWtkpJUFiZ6DrpZCCM9fi3KFw6Cxw3pgelew74MBFz5pOSGesk_O-NUock_PNvyXBx4Qp66VP63XNiGFKWhVDKzkvwnYjtDGkFLHXq-iXJv5oBnqNVi_0H1q9RqtB6YK2WM-2M0pcdH_GLcsiuNoIsCT99Bh1sh5Hi87HQlW74P-f8gv5VZFm</recordid><startdate>20031015</startdate><enddate>20031015</enddate><creator>Ciuffi, Mario</creator><creator>Pisanello, Marcella</creator><creator>Pagliai, Gabriella</creator><creator>Raimondi, Laura</creator><creator>Franchi-Micheli, Sergio</creator><creator>Cantore, Miriam</creator><creator>Mazzetti, Luca</creator><creator>Failli, Paola</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20031015</creationdate><title>Antioxidant protection in cultured corneal cells and whole corneas submitted to UV-B exposure</title><author>Ciuffi, Mario ; Pisanello, Marcella ; Pagliai, Gabriella ; Raimondi, Laura ; Franchi-Micheli, Sergio ; Cantore, Miriam ; Mazzetti, Luca ; Failli, Paola</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c486t-cc5977505e9a09f535321d8ebde3dedc0224afe75f902023f14935dacd5bf8a73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Alveolar macrophages</topic><topic>Animals</topic><topic>Antioxidants - pharmacology</topic><topic>Cells, Cultured</topic><topic>Cornea</topic><topic>Cornea - cytology</topic><topic>Cornea - drug effects</topic><topic>Cornea - radiation effects</topic><topic>Macrophages - physiology</topic><topic>Melatonin</topic><topic>Oxazines - pharmacology</topic><topic>Pirenoxine</topic><topic>Rabbits</topic><topic>Reactive Oxygen Species</topic><topic>Spectrometry, Fluorescence</topic><topic>Ultraviolet Rays</topic><topic>UV-B</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ciuffi, Mario</creatorcontrib><creatorcontrib>Pisanello, Marcella</creatorcontrib><creatorcontrib>Pagliai, Gabriella</creatorcontrib><creatorcontrib>Raimondi, Laura</creatorcontrib><creatorcontrib>Franchi-Micheli, Sergio</creatorcontrib><creatorcontrib>Cantore, Miriam</creatorcontrib><creatorcontrib>Mazzetti, Luca</creatorcontrib><creatorcontrib>Failli, Paola</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of photochemistry and photobiology. B, Biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ciuffi, Mario</au><au>Pisanello, Marcella</au><au>Pagliai, Gabriella</au><au>Raimondi, Laura</au><au>Franchi-Micheli, Sergio</au><au>Cantore, Miriam</au><au>Mazzetti, Luca</au><au>Failli, Paola</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antioxidant protection in cultured corneal cells and whole corneas submitted to UV-B exposure</atitle><jtitle>Journal of photochemistry and photobiology. B, Biology</jtitle><addtitle>J Photochem Photobiol B</addtitle><date>2003-10-15</date><risdate>2003</risdate><volume>71</volume><issue>1</issue><spage>59</spage><epage>68</epage><pages>59-68</pages><issn>1011-1344</issn><eissn>1873-2682</eissn><abstract>Several corneal pathologies are characterized by the presence of reactive oxygen species (ROS); therefore, we evaluated the protection afforded by pirenoxine and melatonin to corneal cell culture and whole rabbit cornea from ultraviolet exposure and other oxidant systems.
Rabbit cornea cell (SIRC) plates and whole corneas were exposed to UV-B (80 or 800 mJ/cm
2) or incubated with fMLP-stimulated autologous macrophages, in the presence or absence of pirenoxine or melatonin (10
−5 M). The protective activity of compounds was assessed by measuring superoxide anion formation, inhibition of oxidation and mitochondrial viability. Moreover the ex vivo protective effect of pirenoxine and melatonin was verified in the whole cornea submitted to UV-B exposure in vitro.
Our experimental data demonstrate that pirenoxine and melatonin were able to inhibit the superoxide formation and oxidative effect in cell culture and whole rabbit corneas submitted to UV-B exposure or to incubation with fMLP-stimulated autologous macrophages.
Mitochondrial viability was restored in epithelial cells of rabbit cornea but not in SIRCs.
Moreover, both compounds are also able to increase ex vivo epithelial corneal cell defences against the in vitro UV-B induced lipid peroxidation.</abstract><cop>Switzerland</cop><pub>Elsevier B.V</pub><pmid>14705640</pmid><doi>10.1016/j.jphotobiol.2003.07.004</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1011-1344 |
| ispartof | Journal of photochemistry and photobiology. B, Biology, 2003-10, Vol.71 (1), p.59-68 |
| issn | 1011-1344 1873-2682 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_71498522 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Alveolar macrophages Animals Antioxidants - pharmacology Cells, Cultured Cornea Cornea - cytology Cornea - drug effects Cornea - radiation effects Macrophages - physiology Melatonin Oxazines - pharmacology Pirenoxine Rabbits Reactive Oxygen Species Spectrometry, Fluorescence Ultraviolet Rays UV-B |
| title | Antioxidant protection in cultured corneal cells and whole corneas submitted to UV-B exposure |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-02T06%3A46%3A24IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Antioxidant%20protection%20in%20cultured%20corneal%20cells%20and%20whole%20corneas%20submitted%20to%20UV-B%20exposure&rft.jtitle=Journal%20of%20photochemistry%20and%20photobiology.%20B,%20Biology&rft.au=Ciuffi,%20Mario&rft.date=2003-10-15&rft.volume=71&rft.issue=1&rft.spage=59&rft.epage=68&rft.pages=59-68&rft.issn=1011-1344&rft.eissn=1873-2682&rft_id=info:doi/10.1016/j.jphotobiol.2003.07.004&rft_dat=%3Cproquest_cross%3E71498522%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=71498522&rft_id=info:pmid/14705640&rft_els_id=S1011134403001131&rfr_iscdi=true |