Peptide profiling of a single Locusta migratoria corpus cardiacum by nano-LC tandem mass spectrometry
The pars intercerebralis–corpora cardiaca complex in insects is the functional equivalent of the vertebrate brain-pituitary axis. During the past few decades more than 40 neuropeptides have been isolated from the locust brain-corpus cardiacum complex. Tedious and time-consuming successive purificati...
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| Veröffentlicht in: | Peptides (New York, N.Y. : 1980) N.Y. : 1980), 2003-10, Vol.24 (10), p.1475-1485 |
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| container_title | Peptides (New York, N.Y. : 1980) |
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| creator | Baggerman, G. Clynen, E. Huybrechts, J. Verleyen, P. Clerens, S. De Loof, A. Schoofs, L. |
| description | The pars intercerebralis–corpora cardiaca complex in insects is the functional equivalent of the vertebrate brain-pituitary axis. During the past few decades more than 40 neuropeptides have been isolated from the locust brain-corpus cardiacum complex. Tedious and time-consuming successive purification rounds of large tissue extracts were necessary to achieve the purification and sequencing of most of these signal molecules. Nowadays, the combination of nanoscale liquid chromatography and the very sensitive tandem mass spectrometry allows us to identify and sequence peptides in very low concentration directly from tissue extracts. In this manuscript, we review previous data on the peptidome analysis of the locust corpora cardiaca, with emphasis on AKH processing. In addition, we report the peptide profiling of a single corpus cardiacum from
Locusta migratoria. 23 peptides were isolated and sequenced in a single nano-LC-MS/MS experiment, demonstrating the sensitivity and effectiveness of mass spectrometry in peptide research. |
| doi_str_mv | 10.1016/j.peptides.2003.08.014 |
| format | Article |
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Locusta migratoria. 23 peptides were isolated and sequenced in a single nano-LC-MS/MS experiment, demonstrating the sensitivity and effectiveness of mass spectrometry in peptide research.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Chromatography, Liquid</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Grasshoppers - chemistry</subject><subject>Insect</subject><subject>Insect Hormones - analysis</subject><subject>Insect Hormones - chemistry</subject><subject>Insect Proteins - analysis</subject><subject>Insect Proteins - chemistry</subject><subject>Locusta migratoria</subject><subject>Mass Spectrometry</subject><subject>Molecular Weight</subject><subject>Nanotechnology</subject><subject>Neurosecretory Systems - chemistry</subject><subject>Peptides - analysis</subject><subject>Peptides - chemistry</subject><subject>Peptidomics</subject><subject>Proteomics</subject><subject>Q-TOF</subject><subject>Vertebrates: endocrinology</subject><issn>0196-9781</issn><issn>1873-5169</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcuO1DAQRS0EYpqBXxh5A7sE23H82IFaw0NqCRawttzlysitJA52gtR_j0fdaJazqirp1OteQu44aznj6uOpXXBZY8DSCsa6lpmWcfmC7LjRXdNzZV-SHeNWNVYbfkPelHJijElpzWtyw6VmqhdqR_DnZQxdchriGOcHmgbqaanZiPSQYCurp1N8yH5NOXoKKS9boeBziB62iR7PdPZzag57uvo54EQnXwotC8Ka04RrPr8lrwY_Fnx3jbfk95f7X_tvzeHH1-_7z4cGpOjXBoQMKhgGve4l6zrWm6HWIAE0CCG0GKyWOAhmjyIcgVmjLBedYEYFP2B3Sz5c5tZv_mxYVjfFAjiOfsa0Fad5_b8T6lmQW8Et17yC6gJCTqVkHNyS4-Tz2XHmHp1wJ_ffCffohGPGVSdq4911w3acMDy1XaWvwPsr4Av4cch-hlieuF722mpduU8XDqtwfyNmVyDiDBhirgq7kOJzt_wDX9mq_g</recordid><startdate>20031001</startdate><enddate>20031001</enddate><creator>Baggerman, G.</creator><creator>Clynen, E.</creator><creator>Huybrechts, J.</creator><creator>Verleyen, P.</creator><creator>Clerens, S.</creator><creator>De Loof, A.</creator><creator>Schoofs, L.</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7X8</scope></search><sort><creationdate>20031001</creationdate><title>Peptide profiling of a single Locusta migratoria corpus cardiacum by nano-LC tandem mass spectrometry</title><author>Baggerman, G. ; Clynen, E. ; Huybrechts, J. ; Verleyen, P. ; Clerens, S. ; De Loof, A. ; Schoofs, L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c425t-c24d6d80c5754033058f6d8c4cc7c22272f974ef209b2dbc098691232086dafe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Chromatography, Liquid</topic><topic>Fundamental and applied biological sciences. 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| subjects | Animals Biological and medical sciences Chromatography, Liquid Fundamental and applied biological sciences. Psychology Grasshoppers - chemistry Insect Insect Hormones - analysis Insect Hormones - chemistry Insect Proteins - analysis Insect Proteins - chemistry Locusta migratoria Mass Spectrometry Molecular Weight Nanotechnology Neurosecretory Systems - chemistry Peptides - analysis Peptides - chemistry Peptidomics Proteomics Q-TOF Vertebrates: endocrinology |
| title | Peptide profiling of a single Locusta migratoria corpus cardiacum by nano-LC tandem mass spectrometry |
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