Proteome Analysis of Hepatocellular Carcinoma
Development of hepatocellular carcinoma (HCC) is a complex process involving multiple changes in gene expression and usually occurs in the presence of liver cirrhosis. In this research, we observed proteome alterations of three tissue types isolated from livers of HCC patients: normal, cirrhotic, an...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2002-03, Vol.291 (4), p.1031-1037 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Lim, Seung Oe Park, Sung-Jun Kim, Won Park, Sung Gyoo Kim, Hie-Joon Kim, Yong-Il Sohn, Tae-Sung Noh, Jae-Hyung Jung, Guhung |
| description | Development of hepatocellular carcinoma (HCC) is a complex process involving multiple changes in gene expression and usually occurs in the presence of liver cirrhosis. In this research, we observed proteome alterations of three tissue types isolated from livers of HCC patients: normal, cirrhotic, and tumorous tissue. Proteome alterations were observed using two-dimensional polyacrylamide gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Comparing the tissue types with each other, a significant change in expression level was found in 21 proteins. Of these proteins, sarcosine dehydrogenase, liver carboxylesterase, peptidyl-prolyl isomerase A, and lamin B1 are considered novel HCC marker candidates. In particular, lamin B1 may be considered as a marker for cirrhosis, because its expression level changes considerably in cirrhotic tissue compared with normal tissue. The proteins revealed in this experiment can be used in the future for studies pertaining to hepatocarcinogenesis, or as diagnostic markers and therapeutic targets for HCC. |
| doi_str_mv | 10.1006/bbrc.2002.6547 |
| format | Article |
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In this research, we observed proteome alterations of three tissue types isolated from livers of HCC patients: normal, cirrhotic, and tumorous tissue. Proteome alterations were observed using two-dimensional polyacrylamide gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Comparing the tissue types with each other, a significant change in expression level was found in 21 proteins. Of these proteins, sarcosine dehydrogenase, liver carboxylesterase, peptidyl-prolyl isomerase A, and lamin B1 are considered novel HCC marker candidates. In particular, lamin B1 may be considered as a marker for cirrhosis, because its expression level changes considerably in cirrhotic tissue compared with normal tissue. The proteins revealed in this experiment can be used in the future for studies pertaining to hepatocarcinogenesis, or as diagnostic markers and therapeutic targets for HCC.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1006/bbrc.2002.6547</identifier><identifier>PMID: 11866469</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Biomarkers, Tumor - analysis ; Carboxylic Ester Hydrolases - analysis ; Carcinoma, Hepatocellular - metabolism ; cirrhosis ; Cyclophilin A - analysis ; Down-Regulation ; Electrophoresis, Gel, Two-Dimensional ; hepatocellular carcinoma ; Humans ; lamin B1 ; Lamin Type B ; Lamins ; Liver - metabolism ; liver carboxylesterase ; Liver Cirrhosis - metabolism ; Liver Neoplasms - metabolism ; mass spectrometry ; matrix-assisted laser desorption/ionization time-of-flight ; Neoplasm Proteins - analysis ; Neoplasm Proteins - metabolism ; Nuclear Proteins - analysis ; Oxidoreductases, N-Demethylating - analysis ; peptidyl-prolyl isomerase A ; Proteome - analysis ; Proteome - metabolism ; Sarcosine Dehydrogenase ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; two-dimensional polyacrylamide gel electrophoresis ; Up-Regulation</subject><ispartof>Biochemical and biophysical research communications, 2002-03, Vol.291 (4), p.1031-1037</ispartof><rights>2002 Elsevier Science (USA)</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c406t-47ab339aa8541b166af886160d5ce16b7a02ae082a1edf2594f4ec6415b3b0e33</citedby><cites>FETCH-LOGICAL-c406t-47ab339aa8541b166af886160d5ce16b7a02ae082a1edf2594f4ec6415b3b0e33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0006291X02965472$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11866469$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lim, Seung Oe</creatorcontrib><creatorcontrib>Park, Sung-Jun</creatorcontrib><creatorcontrib>Kim, Won</creatorcontrib><creatorcontrib>Park, Sung Gyoo</creatorcontrib><creatorcontrib>Kim, Hie-Joon</creatorcontrib><creatorcontrib>Kim, Yong-Il</creatorcontrib><creatorcontrib>Sohn, Tae-Sung</creatorcontrib><creatorcontrib>Noh, Jae-Hyung</creatorcontrib><creatorcontrib>Jung, Guhung</creatorcontrib><title>Proteome Analysis of Hepatocellular Carcinoma</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Development of hepatocellular carcinoma (HCC) is a complex process involving multiple changes in gene expression and usually occurs in the presence of liver cirrhosis. In this research, we observed proteome alterations of three tissue types isolated from livers of HCC patients: normal, cirrhotic, and tumorous tissue. Proteome alterations were observed using two-dimensional polyacrylamide gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Comparing the tissue types with each other, a significant change in expression level was found in 21 proteins. Of these proteins, sarcosine dehydrogenase, liver carboxylesterase, peptidyl-prolyl isomerase A, and lamin B1 are considered novel HCC marker candidates. In particular, lamin B1 may be considered as a marker for cirrhosis, because its expression level changes considerably in cirrhotic tissue compared with normal tissue. The proteins revealed in this experiment can be used in the future for studies pertaining to hepatocarcinogenesis, or as diagnostic markers and therapeutic targets for HCC.</description><subject>Biomarkers, Tumor - analysis</subject><subject>Carboxylic Ester Hydrolases - analysis</subject><subject>Carcinoma, Hepatocellular - metabolism</subject><subject>cirrhosis</subject><subject>Cyclophilin A - analysis</subject><subject>Down-Regulation</subject><subject>Electrophoresis, Gel, Two-Dimensional</subject><subject>hepatocellular carcinoma</subject><subject>Humans</subject><subject>lamin B1</subject><subject>Lamin Type B</subject><subject>Lamins</subject><subject>Liver - metabolism</subject><subject>liver carboxylesterase</subject><subject>Liver Cirrhosis - metabolism</subject><subject>Liver Neoplasms - metabolism</subject><subject>mass spectrometry</subject><subject>matrix-assisted laser desorption/ionization time-of-flight</subject><subject>Neoplasm Proteins - analysis</subject><subject>Neoplasm Proteins - metabolism</subject><subject>Nuclear Proteins - analysis</subject><subject>Oxidoreductases, N-Demethylating - analysis</subject><subject>peptidyl-prolyl isomerase A</subject><subject>Proteome - analysis</subject><subject>Proteome - metabolism</subject><subject>Sarcosine Dehydrogenase</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</subject><subject>two-dimensional polyacrylamide gel electrophoresis</subject><subject>Up-Regulation</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kEFLxDAQRoMo7rp69Sg9eWudadO0PS6LusKCHhS8hSSdQqRt1qQV9t_bsguePM0c3vcx8xi7RUgQQDxo7U2SAqSJyHlxxpYIFcQpAj9nS5iIOK3wc8GuQvgCQOSiumQLxFKIaV2y-M27gVxH0bpX7SHYELkm2tJeDc5Q246t8tFGeWN716lrdtGoNtDNaa7Yx9Pj-2Yb716fXzbrXWw4iCHmhdJZVilV5hw1CqGashQooM4NodCFglQRlKlCqps0r3jDyQiOuc40UJat2P2xd-_d90hhkJ0N8zmqJzcGWSCvRMlhApMjaLwLwVMj9952yh8kgpwFyVmQnAXJWdAUuDs1j7qj-g8_GZmA8gjQ9N-PJS-DsdQbqq0nM8ja2f-6fwEUgHOA</recordid><startdate>20020308</startdate><enddate>20020308</enddate><creator>Lim, Seung Oe</creator><creator>Park, Sung-Jun</creator><creator>Kim, Won</creator><creator>Park, Sung Gyoo</creator><creator>Kim, Hie-Joon</creator><creator>Kim, Yong-Il</creator><creator>Sohn, Tae-Sung</creator><creator>Noh, Jae-Hyung</creator><creator>Jung, Guhung</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20020308</creationdate><title>Proteome Analysis of Hepatocellular Carcinoma</title><author>Lim, Seung Oe ; Park, Sung-Jun ; Kim, Won ; Park, Sung Gyoo ; Kim, Hie-Joon ; Kim, Yong-Il ; Sohn, Tae-Sung ; Noh, Jae-Hyung ; Jung, Guhung</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c406t-47ab339aa8541b166af886160d5ce16b7a02ae082a1edf2594f4ec6415b3b0e33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Biomarkers, Tumor - analysis</topic><topic>Carboxylic Ester Hydrolases - analysis</topic><topic>Carcinoma, Hepatocellular - metabolism</topic><topic>cirrhosis</topic><topic>Cyclophilin A - analysis</topic><topic>Down-Regulation</topic><topic>Electrophoresis, Gel, Two-Dimensional</topic><topic>hepatocellular carcinoma</topic><topic>Humans</topic><topic>lamin B1</topic><topic>Lamin Type B</topic><topic>Lamins</topic><topic>Liver - metabolism</topic><topic>liver carboxylesterase</topic><topic>Liver Cirrhosis - metabolism</topic><topic>Liver Neoplasms - metabolism</topic><topic>mass spectrometry</topic><topic>matrix-assisted laser desorption/ionization time-of-flight</topic><topic>Neoplasm Proteins - analysis</topic><topic>Neoplasm Proteins - metabolism</topic><topic>Nuclear Proteins - analysis</topic><topic>Oxidoreductases, N-Demethylating - analysis</topic><topic>peptidyl-prolyl isomerase A</topic><topic>Proteome - analysis</topic><topic>Proteome - metabolism</topic><topic>Sarcosine Dehydrogenase</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</topic><topic>two-dimensional polyacrylamide gel electrophoresis</topic><topic>Up-Regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lim, Seung Oe</creatorcontrib><creatorcontrib>Park, Sung-Jun</creatorcontrib><creatorcontrib>Kim, Won</creatorcontrib><creatorcontrib>Park, Sung Gyoo</creatorcontrib><creatorcontrib>Kim, Hie-Joon</creatorcontrib><creatorcontrib>Kim, Yong-Il</creatorcontrib><creatorcontrib>Sohn, Tae-Sung</creatorcontrib><creatorcontrib>Noh, Jae-Hyung</creatorcontrib><creatorcontrib>Jung, Guhung</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lim, Seung Oe</au><au>Park, Sung-Jun</au><au>Kim, Won</au><au>Park, Sung Gyoo</au><au>Kim, Hie-Joon</au><au>Kim, Yong-Il</au><au>Sohn, Tae-Sung</au><au>Noh, Jae-Hyung</au><au>Jung, Guhung</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Proteome Analysis of Hepatocellular Carcinoma</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2002-03-08</date><risdate>2002</risdate><volume>291</volume><issue>4</issue><spage>1031</spage><epage>1037</epage><pages>1031-1037</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Development of hepatocellular carcinoma (HCC) is a complex process involving multiple changes in gene expression and usually occurs in the presence of liver cirrhosis. In this research, we observed proteome alterations of three tissue types isolated from livers of HCC patients: normal, cirrhotic, and tumorous tissue. Proteome alterations were observed using two-dimensional polyacrylamide gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Comparing the tissue types with each other, a significant change in expression level was found in 21 proteins. Of these proteins, sarcosine dehydrogenase, liver carboxylesterase, peptidyl-prolyl isomerase A, and lamin B1 are considered novel HCC marker candidates. In particular, lamin B1 may be considered as a marker for cirrhosis, because its expression level changes considerably in cirrhotic tissue compared with normal tissue. The proteins revealed in this experiment can be used in the future for studies pertaining to hepatocarcinogenesis, or as diagnostic markers and therapeutic targets for HCC.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>11866469</pmid><doi>10.1006/bbrc.2002.6547</doi><tpages>7</tpages></addata></record> |
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| subjects | Biomarkers, Tumor - analysis Carboxylic Ester Hydrolases - analysis Carcinoma, Hepatocellular - metabolism cirrhosis Cyclophilin A - analysis Down-Regulation Electrophoresis, Gel, Two-Dimensional hepatocellular carcinoma Humans lamin B1 Lamin Type B Lamins Liver - metabolism liver carboxylesterase Liver Cirrhosis - metabolism Liver Neoplasms - metabolism mass spectrometry matrix-assisted laser desorption/ionization time-of-flight Neoplasm Proteins - analysis Neoplasm Proteins - metabolism Nuclear Proteins - analysis Oxidoreductases, N-Demethylating - analysis peptidyl-prolyl isomerase A Proteome - analysis Proteome - metabolism Sarcosine Dehydrogenase Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization two-dimensional polyacrylamide gel electrophoresis Up-Regulation |
| title | Proteome Analysis of Hepatocellular Carcinoma |
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