Strategy to sequence the genome of Corynebacterium glutamicum ATCC 13032: use of a cosmid and a bacterial artificial chromosome library

The initial strategy of the Corynebacterium glutamicum genome project was to sequence overlapping inserts of an ordered cosmid library. High-density colony grids of approximately 28 genome equivalents were used for the identification of overlapping clones by Southern hybridization. Altogether 18 con...

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Veröffentlicht in:	Journal of biotechnology 2002-04, Vol.95 (1), p.25-38
Hauptverfasser:	Tauch, Andreas, Homann, Iris, Mormann, Sascha, Rüberg, Silvia, Billault, Alain, Bathe, Brigitte, Brand, Sven, Brockmann-Gretza, Olaf, Rückert, Christian, Schischka, Natalie, Wrenger, Carsten, Hoheisel, Jörg, Möckel, Bettina, Huthmacher, Klaus, Pfefferle, Walter, Pühler, Alfred, Kalinowski, Jörn
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial artificial chromosome library Biological and medical sciences Biotechnology Chromosomes, Artificial, Bacterial - genetics Chromosomes, Bacterial - genetics Cloning, Molecular Contig Mapping - methods Corynebacterium - genetics Corynebacterium glutamicum Cosmids - genetics Fundamental and applied biological sciences. Psychology Genes, Bacterial - genetics Genome mapping Genome sequence Genome, Bacterial Genomic Library Genomics - methods Sequence Analysis, DNA - methods
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