Apoptosis mediated by the Fas system in the fulminant hepatitis by hepatitis B virus
The pathogenesis of the fulminant hepatitis B is poorly understood and both viral factors and the hosts immune response play a role. Previous studies in liver tissues of patients with chronic hepatitis B showed overexpression of Fas antigen and this was correlated with the activity of the hepatitis....
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| Veröffentlicht in: | Journal of viral hepatitis 2002-03, Vol.9 (2), p.107-113 |
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| container_title | Journal of viral hepatitis |
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| creator | Rivero, M. Crespo, J. Fábrega, E. Casafont, F. Mayorga, M. Gomez-Fleitas, M. Pons-Romero, F. |
| description | The pathogenesis of the fulminant hepatitis B is poorly understood and both viral factors and the hosts immune response play a role. Previous studies in liver tissues of patients with chronic hepatitis B showed overexpression of Fas antigen and this was correlated with the activity of the hepatitis. The present study was done to determine the role of Fas in fulminant hepatitis B and the virological characteristics of hepatitis B infection.
We studied three patients with fulminant hepatitis B. HBV‐DNA was detected by dot‐blot hybridization and polymerase chain reaction. The S and C gene were sequenced. Levels of serum soluble Fas antigen were detected by enzymoimmunoassays procedure. Apoptosis was determined by the TUNEL technique. Fas antigen expression was evaluated by a immunoperoxidase method. Ten healthy subjects acted as controls.
The three patients showed a high expression of Fas antigen particularly among infiltrating lymphocytes; in these areas we also found many cells with in situ DNA nick labelling signals in the nuclei of most viable hepatocytes. Serum levels of soluble Fas antigen were higher in patients with fulminant hepatitis B than in controls. No specific genome mutations of hepatitis B virus were found.
These data suggest that the Fas system involved in the liver injury of patients with fulminant hepatitis B. |
| doi_str_mv | 10.1046/j.1365-2893.2002.00338.x |
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We studied three patients with fulminant hepatitis B. HBV‐DNA was detected by dot‐blot hybridization and polymerase chain reaction. The S and C gene were sequenced. Levels of serum soluble Fas antigen were detected by enzymoimmunoassays procedure. Apoptosis was determined by the TUNEL technique. Fas antigen expression was evaluated by a immunoperoxidase method. Ten healthy subjects acted as controls.
The three patients showed a high expression of Fas antigen particularly among infiltrating lymphocytes; in these areas we also found many cells with in situ DNA nick labelling signals in the nuclei of most viable hepatocytes. Serum levels of soluble Fas antigen were higher in patients with fulminant hepatitis B than in controls. No specific genome mutations of hepatitis B virus were found.
These data suggest that the Fas system involved in the liver injury of patients with fulminant hepatitis B.</description><identifier>ISSN: 1352-0504</identifier><identifier>EISSN: 1365-2893</identifier><identifier>DOI: 10.1046/j.1365-2893.2002.00338.x</identifier><identifier>PMID: 11876792</identifier><language>eng</language><publisher>Oxford UK: Blackwell Science Ltd</publisher><subject>AC gene ; Adult ; Aged ; Amino Acid Sequence ; Apoptosis ; AS gene ; DNA, Viral - analysis ; Fas antigen ; fas Receptor - biosynthesis ; fulminant hepatitis ; Hepatitis B - blood ; Hepatitis B - immunology ; Hepatitis B - pathology ; Hepatitis B - virology ; Hepatitis B Antibodies - blood ; Hepatitis B Antibodies - immunology ; Hepatitis B Core Antigens - genetics ; Hepatitis B Core Antigens - immunology ; Hepatitis B e Antigens - immunology ; Hepatitis B Surface Antigens - genetics ; Hepatitis B Surface Antigens - immunology ; Hepatitis B virus ; Hepatitis B virus - genetics ; Hepatitis B virus - immunology ; Hepatocytes - metabolism ; Humans ; In Situ Nick-End Labeling ; Liver Failure - pathology ; Liver Failure - virology ; Male ; Middle Aged ; Molecular Sequence Data ; Sequence Analysis, DNA ; Solubility ; soluble serum Fas ; TUNEL</subject><ispartof>Journal of viral hepatitis, 2002-03, Vol.9 (2), p.107-113</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4998-21e7dd6c4d6d2e26898990cd91e00b817c26ffe4b07d06ae4cba6b295068f1563</citedby><cites>FETCH-LOGICAL-c4998-21e7dd6c4d6d2e26898990cd91e00b817c26ffe4b07d06ae4cba6b295068f1563</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1365-2893.2002.00338.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1365-2893.2002.00338.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11876792$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rivero, M.</creatorcontrib><creatorcontrib>Crespo, J.</creatorcontrib><creatorcontrib>Fábrega, E.</creatorcontrib><creatorcontrib>Casafont, F.</creatorcontrib><creatorcontrib>Mayorga, M.</creatorcontrib><creatorcontrib>Gomez-Fleitas, M.</creatorcontrib><creatorcontrib>Pons-Romero, F.</creatorcontrib><title>Apoptosis mediated by the Fas system in the fulminant hepatitis by hepatitis B virus</title><title>Journal of viral hepatitis</title><addtitle>J Viral Hepat</addtitle><description>The pathogenesis of the fulminant hepatitis B is poorly understood and both viral factors and the hosts immune response play a role. Previous studies in liver tissues of patients with chronic hepatitis B showed overexpression of Fas antigen and this was correlated with the activity of the hepatitis. The present study was done to determine the role of Fas in fulminant hepatitis B and the virological characteristics of hepatitis B infection.
We studied three patients with fulminant hepatitis B. HBV‐DNA was detected by dot‐blot hybridization and polymerase chain reaction. The S and C gene were sequenced. Levels of serum soluble Fas antigen were detected by enzymoimmunoassays procedure. Apoptosis was determined by the TUNEL technique. Fas antigen expression was evaluated by a immunoperoxidase method. Ten healthy subjects acted as controls.
The three patients showed a high expression of Fas antigen particularly among infiltrating lymphocytes; in these areas we also found many cells with in situ DNA nick labelling signals in the nuclei of most viable hepatocytes. Serum levels of soluble Fas antigen were higher in patients with fulminant hepatitis B than in controls. No specific genome mutations of hepatitis B virus were found.
These data suggest that the Fas system involved in the liver injury of patients with fulminant hepatitis B.</description><subject>AC gene</subject><subject>Adult</subject><subject>Aged</subject><subject>Amino Acid Sequence</subject><subject>Apoptosis</subject><subject>AS gene</subject><subject>DNA, Viral - analysis</subject><subject>Fas antigen</subject><subject>fas Receptor - biosynthesis</subject><subject>fulminant hepatitis</subject><subject>Hepatitis B - blood</subject><subject>Hepatitis B - immunology</subject><subject>Hepatitis B - pathology</subject><subject>Hepatitis B - virology</subject><subject>Hepatitis B Antibodies - blood</subject><subject>Hepatitis B Antibodies - immunology</subject><subject>Hepatitis B Core Antigens - genetics</subject><subject>Hepatitis B Core Antigens - immunology</subject><subject>Hepatitis B e Antigens - immunology</subject><subject>Hepatitis B Surface Antigens - genetics</subject><subject>Hepatitis B Surface Antigens - immunology</subject><subject>Hepatitis B virus</subject><subject>Hepatitis B virus - genetics</subject><subject>Hepatitis B virus - immunology</subject><subject>Hepatocytes - metabolism</subject><subject>Humans</subject><subject>In Situ Nick-End Labeling</subject><subject>Liver Failure - pathology</subject><subject>Liver Failure - virology</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Molecular Sequence Data</subject><subject>Sequence Analysis, DNA</subject><subject>Solubility</subject><subject>soluble serum Fas</subject><subject>TUNEL</subject><issn>1352-0504</issn><issn>1365-2893</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkV1P2zAUhi0EooztL0y-4i7ZsZ34Q-KG8dGCENsErNJuLCdxhLukCXGytf8eh1b0Eq58bL_Pa-kxQphATCDh3xYxYTyNqFQspgA0BmBMxqs9dPR2sT_OKY0ghWSCPnm_ACCMpuQQTQiRggtFj9DDWdu0feOdx7UtnOltgbM17p8svjIe-7XvbY3d8vWkHKraLc2yx0-2Nb3rAxXCu813_M91g_-MDkpTeftlux6jx6vLh_NZdPtjen1-dhvliVIyosSKouB5UvCCWsqlkkpBXihiATJJRE55WdokA1EANzbJM8MzqlLgsiQpZ8foZNPbds3zYH2va-dzW1VmaZvBa0GSIEKKd4NEUpFKNTbKTTDvGu87W-q2c7Xp1pqAHtXrhR4N69GwHtXrV_V6FdCv2zeGLKjcgVvXIXC6Cfx3lV1_uFjf_J6FIeDRBnfhS1ZvuOn-ai6YSPX8bqrlTzb_dX8x13_YC1cfoUQ</recordid><startdate>200203</startdate><enddate>200203</enddate><creator>Rivero, M.</creator><creator>Crespo, J.</creator><creator>Fábrega, E.</creator><creator>Casafont, F.</creator><creator>Mayorga, M.</creator><creator>Gomez-Fleitas, M.</creator><creator>Pons-Romero, F.</creator><general>Blackwell Science Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>200203</creationdate><title>Apoptosis mediated by the Fas system in the fulminant hepatitis by hepatitis B virus</title><author>Rivero, M. ; Crespo, J. ; Fábrega, E. ; Casafont, F. ; Mayorga, M. ; Gomez-Fleitas, M. ; Pons-Romero, F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4998-21e7dd6c4d6d2e26898990cd91e00b817c26ffe4b07d06ae4cba6b295068f1563</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>AC gene</topic><topic>Adult</topic><topic>Aged</topic><topic>Amino Acid Sequence</topic><topic>Apoptosis</topic><topic>AS gene</topic><topic>DNA, Viral - analysis</topic><topic>Fas antigen</topic><topic>fas Receptor - biosynthesis</topic><topic>fulminant hepatitis</topic><topic>Hepatitis B - blood</topic><topic>Hepatitis B - immunology</topic><topic>Hepatitis B - pathology</topic><topic>Hepatitis B - virology</topic><topic>Hepatitis B Antibodies - blood</topic><topic>Hepatitis B Antibodies - immunology</topic><topic>Hepatitis B Core Antigens - genetics</topic><topic>Hepatitis B Core Antigens - immunology</topic><topic>Hepatitis B e Antigens - immunology</topic><topic>Hepatitis B Surface Antigens - genetics</topic><topic>Hepatitis B Surface Antigens - immunology</topic><topic>Hepatitis B virus</topic><topic>Hepatitis B virus - genetics</topic><topic>Hepatitis B virus - immunology</topic><topic>Hepatocytes - metabolism</topic><topic>Humans</topic><topic>In Situ Nick-End Labeling</topic><topic>Liver Failure - pathology</topic><topic>Liver Failure - virology</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Molecular Sequence Data</topic><topic>Sequence Analysis, DNA</topic><topic>Solubility</topic><topic>soluble serum Fas</topic><topic>TUNEL</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rivero, M.</creatorcontrib><creatorcontrib>Crespo, J.</creatorcontrib><creatorcontrib>Fábrega, E.</creatorcontrib><creatorcontrib>Casafont, F.</creatorcontrib><creatorcontrib>Mayorga, M.</creatorcontrib><creatorcontrib>Gomez-Fleitas, M.</creatorcontrib><creatorcontrib>Pons-Romero, F.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of viral hepatitis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rivero, M.</au><au>Crespo, J.</au><au>Fábrega, E.</au><au>Casafont, F.</au><au>Mayorga, M.</au><au>Gomez-Fleitas, M.</au><au>Pons-Romero, F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Apoptosis mediated by the Fas system in the fulminant hepatitis by hepatitis B virus</atitle><jtitle>Journal of viral hepatitis</jtitle><addtitle>J Viral Hepat</addtitle><date>2002-03</date><risdate>2002</risdate><volume>9</volume><issue>2</issue><spage>107</spage><epage>113</epage><pages>107-113</pages><issn>1352-0504</issn><eissn>1365-2893</eissn><abstract>The pathogenesis of the fulminant hepatitis B is poorly understood and both viral factors and the hosts immune response play a role. Previous studies in liver tissues of patients with chronic hepatitis B showed overexpression of Fas antigen and this was correlated with the activity of the hepatitis. The present study was done to determine the role of Fas in fulminant hepatitis B and the virological characteristics of hepatitis B infection.
We studied three patients with fulminant hepatitis B. HBV‐DNA was detected by dot‐blot hybridization and polymerase chain reaction. The S and C gene were sequenced. Levels of serum soluble Fas antigen were detected by enzymoimmunoassays procedure. Apoptosis was determined by the TUNEL technique. Fas antigen expression was evaluated by a immunoperoxidase method. Ten healthy subjects acted as controls.
The three patients showed a high expression of Fas antigen particularly among infiltrating lymphocytes; in these areas we also found many cells with in situ DNA nick labelling signals in the nuclei of most viable hepatocytes. Serum levels of soluble Fas antigen were higher in patients with fulminant hepatitis B than in controls. No specific genome mutations of hepatitis B virus were found.
These data suggest that the Fas system involved in the liver injury of patients with fulminant hepatitis B.</abstract><cop>Oxford UK</cop><pub>Blackwell Science Ltd</pub><pmid>11876792</pmid><doi>10.1046/j.1365-2893.2002.00338.x</doi><tpages>7</tpages></addata></record> |
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| subjects | AC gene Adult Aged Amino Acid Sequence Apoptosis AS gene DNA, Viral - analysis Fas antigen fas Receptor - biosynthesis fulminant hepatitis Hepatitis B - blood Hepatitis B - immunology Hepatitis B - pathology Hepatitis B - virology Hepatitis B Antibodies - blood Hepatitis B Antibodies - immunology Hepatitis B Core Antigens - genetics Hepatitis B Core Antigens - immunology Hepatitis B e Antigens - immunology Hepatitis B Surface Antigens - genetics Hepatitis B Surface Antigens - immunology Hepatitis B virus Hepatitis B virus - genetics Hepatitis B virus - immunology Hepatocytes - metabolism Humans In Situ Nick-End Labeling Liver Failure - pathology Liver Failure - virology Male Middle Aged Molecular Sequence Data Sequence Analysis, DNA Solubility soluble serum Fas TUNEL |
| title | Apoptosis mediated by the Fas system in the fulminant hepatitis by hepatitis B virus |
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