Efficient PCR‐based gene disruption in Saccharomyces strains using intergenic primers

An Erratum has been published for this article in Yeast 19(9) 2002, 803. Gene disruptions are a vital tool for understanding Saccharomyces cerevisiae gene function. An arrayed library of gene disruption strains has been produced by a consortium of yeast laboratories; however their use is limited to...

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Veröffentlicht in:	Yeast (Chichester, England) England), 2002-03, Vol.19 (4), p.319-328
Hauptverfasser:	Reid, Robert J.D., Sunjevaric, Ivana, Kedacche, Mehdi, Rothstein, Rodney
Format:	Artikel
Sprache:	eng
Schlagworte:	deletion consortium DNA Primers Gene Deletion gene disruption Kluyveromyces - genetics Kluyveromyces lactis Molecular Biology Polymerase Chain Reaction - methods Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Transformation, Genetic URA3 gene yeast
Online-Zugang:	Volltext
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