RAPD method useful for distinguishing Leishmania species: design of specific primers for L. braziliensis
The technique of Random Amplification Polymorphic DNA allows fragments of the genome to be amplified by means of polymerase chain reaction (PCR) without previous knowledge of their sequences. The protozoa of the genus Leishmania present great genetic variability, making it difficult to characterize...
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| Veröffentlicht in: | Parasitology 2003-12, Vol.127 (6), p.513-517, Article S0031182003004104 |
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| creator | MARTINEZ, E. ALONSO, V. QUISPE, A. THOMAS, M. C. ALONSO, R. PIÑERO, J. E. GONZÁLEZ, A. C. ORTEGA, A. VALLADARES, B. |
| description | The technique of Random Amplification Polymorphic DNA allows fragments of the genome to be amplified by means of polymerase chain reaction (PCR) without previous knowledge of their sequences. The protozoa of the genus Leishmania present great genetic variability, making it difficult to characterize the different species. A method is developed with a single 10-mers long primer, which allows the species L. braziliensis, L. mexicana, L. infantum, L. tropica, L. chagasi, L. amazonensis and L. major to be differentiated. These products amplified by RAPD have also facilitated the design of some primers that amplify L. braziliensis DNA exclusively. |
| doi_str_mv | 10.1017/S0031182003004104 |
| format | Article |
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C. ; ALONSO, R. ; PIÑERO, J. E. ; GONZÁLEZ, A. C. ; ORTEGA, A. ; VALLADARES, B.</creator><creatorcontrib>MARTINEZ, E. ; ALONSO, V. ; QUISPE, A. ; THOMAS, M. C. ; ALONSO, R. ; PIÑERO, J. E. ; GONZÁLEZ, A. C. ; ORTEGA, A. ; VALLADARES, B.</creatorcontrib><description>The technique of Random Amplification Polymorphic DNA allows fragments of the genome to be amplified by means of polymerase chain reaction (PCR) without previous knowledge of their sequences. The protozoa of the genus Leishmania present great genetic variability, making it difficult to characterize the different species. A method is developed with a single 10-mers long primer, which allows the species L. braziliensis, L. mexicana, L. infantum, L. tropica, L. chagasi, L. amazonensis and L. major to be differentiated. These products amplified by RAPD have also facilitated the design of some primers that amplify L. braziliensis DNA exclusively.</description><identifier>ISSN: 0031-1820</identifier><identifier>EISSN: 1469-8161</identifier><identifier>DOI: 10.1017/S0031182003004104</identifier><identifier>PMID: 14700186</identifier><identifier>CODEN: PARAAE</identifier><language>eng</language><publisher>Cambridge, UK: Cambridge University Press</publisher><subject>Animals ; Biological and medical sciences ; Cloning, Molecular ; Deoxyribonucleic acid ; DNA ; DNA Primers ; DNA, Protozoan - chemistry ; DNA, Protozoan - genetics ; E coli ; Fundamental and applied biological sciences. Psychology ; General aspects ; General aspects and techniques. Study of several systematic groups. Models ; Genetic Variation ; Humans ; Invertebrates ; Leishmania - classification ; Leishmania - genetics ; Leishmania amazonensis ; Leishmania braziliensis ; Leishmania chagasi ; Leishmania infantum ; Leishmania mexicana ; Leishmania tropica ; Leishmaniasis - diagnosis ; Methods ; Molecular weight ; Nucleic Acid Hybridization ; PCR ; primer ; Protocol ; Random Amplified Polymorphic DNA Technique - methods ; RAPD ; Temperature</subject><ispartof>Parasitology, 2003-12, Vol.127 (6), p.513-517, Article S0031182003004104</ispartof><rights>2003 Cambridge University Press</rights><rights>2004 INIST-CNRS</rights><rights>Copyright Cambridge University Press Dec 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c467t-1b9639080dbdac9c52c3ca144b2095f4e674be11b17fab1d3482b9536b2d22233</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.cambridge.org/core/product/identifier/S0031182003004104/type/journal_article$$EHTML$$P50$$Gcambridge$$H</linktohtml><link.rule.ids>164,314,776,780,27901,27902,55603</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15378425$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14700186$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>MARTINEZ, E.</creatorcontrib><creatorcontrib>ALONSO, V.</creatorcontrib><creatorcontrib>QUISPE, A.</creatorcontrib><creatorcontrib>THOMAS, M. C.</creatorcontrib><creatorcontrib>ALONSO, R.</creatorcontrib><creatorcontrib>PIÑERO, J. E.</creatorcontrib><creatorcontrib>GONZÁLEZ, A. C.</creatorcontrib><creatorcontrib>ORTEGA, A.</creatorcontrib><creatorcontrib>VALLADARES, B.</creatorcontrib><title>RAPD method useful for distinguishing Leishmania species: design of specific primers for L. braziliensis</title><title>Parasitology</title><addtitle>Parasitology</addtitle><description>The technique of Random Amplification Polymorphic DNA allows fragments of the genome to be amplified by means of polymerase chain reaction (PCR) without previous knowledge of their sequences. The protozoa of the genus Leishmania present great genetic variability, making it difficult to characterize the different species. A method is developed with a single 10-mers long primer, which allows the species L. braziliensis, L. mexicana, L. infantum, L. tropica, L. chagasi, L. amazonensis and L. major to be differentiated. These products amplified by RAPD have also facilitated the design of some primers that amplify L. braziliensis DNA exclusively.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cloning, Molecular</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA Primers</subject><subject>DNA, Protozoan - chemistry</subject><subject>DNA, Protozoan - genetics</subject><subject>E coli</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>General aspects and techniques. Study of several systematic groups. Models</subject><subject>Genetic Variation</subject><subject>Humans</subject><subject>Invertebrates</subject><subject>Leishmania - classification</subject><subject>Leishmania - genetics</subject><subject>Leishmania amazonensis</subject><subject>Leishmania braziliensis</subject><subject>Leishmania chagasi</subject><subject>Leishmania infantum</subject><subject>Leishmania mexicana</subject><subject>Leishmania tropica</subject><subject>Leishmaniasis - diagnosis</subject><subject>Methods</subject><subject>Molecular weight</subject><subject>Nucleic Acid Hybridization</subject><subject>PCR</subject><subject>primer</subject><subject>Protocol</subject><subject>Random Amplified Polymorphic DNA Technique - methods</subject><subject>RAPD</subject><subject>Temperature</subject><issn>0031-1820</issn><issn>1469-8161</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFkctuEzEUhi0EoqHwAGyQhQS7KT62x55hVwpNkSJuhbVlezyJy1yCT0YCnh5PExEJhFgdy_93rj8hj4GdAQP94poxAVDxHBiTwOQdsgCp6qICBXfJYpaLWT8hDxBvGGNKKH6fnIDUjEGlFmTz6fzDa9qH3WZs6IShnTrajok2EXdxWE8RNznQVciP3g7RUtwGHwO-pE3AuB7o2O6_2ujpNsU-JLytsDqjLtmfsYthwIgPyb3WdhgeHeIp-XL55vPFVbF6v3x7cb4qvFR6V4CrlahZxRrXWF_7knvhLUjpOKvLVgalpQsADnRrHTRCVtzVpVCON5xzIU7J833dbRq_TQF3po_oQ9fZIYwTGg2yElzI_4KgaynzHTP49A_wZpzSkJcwPF-bg-BlhmAP-TQiptCa-RY2_TDAzGyW-cusnPPkUHhyfWiOGQd3MvDsAFj0tmuTHXzEI1cKXcnb5sWey6aF7791m74apYUujVp-NPDu6lV1vbw0c2NxGNb2LsVmHY4r_XvcX5SzuKw</recordid><startdate>20031201</startdate><enddate>20031201</enddate><creator>MARTINEZ, E.</creator><creator>ALONSO, V.</creator><creator>QUISPE, A.</creator><creator>THOMAS, M. 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Models</topic><topic>Genetic Variation</topic><topic>Humans</topic><topic>Invertebrates</topic><topic>Leishmania - classification</topic><topic>Leishmania - genetics</topic><topic>Leishmania amazonensis</topic><topic>Leishmania braziliensis</topic><topic>Leishmania chagasi</topic><topic>Leishmania infantum</topic><topic>Leishmania mexicana</topic><topic>Leishmania tropica</topic><topic>Leishmaniasis - diagnosis</topic><topic>Methods</topic><topic>Molecular weight</topic><topic>Nucleic Acid Hybridization</topic><topic>PCR</topic><topic>primer</topic><topic>Protocol</topic><topic>Random Amplified Polymorphic DNA Technique - methods</topic><topic>RAPD</topic><topic>Temperature</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MARTINEZ, E.</creatorcontrib><creatorcontrib>ALONSO, V.</creatorcontrib><creatorcontrib>QUISPE, A.</creatorcontrib><creatorcontrib>THOMAS, M. 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C.</au><au>ALONSO, R.</au><au>PIÑERO, J. E.</au><au>GONZÁLEZ, A. C.</au><au>ORTEGA, A.</au><au>VALLADARES, B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>RAPD method useful for distinguishing Leishmania species: design of specific primers for L. braziliensis</atitle><jtitle>Parasitology</jtitle><addtitle>Parasitology</addtitle><date>2003-12-01</date><risdate>2003</risdate><volume>127</volume><issue>6</issue><spage>513</spage><epage>517</epage><pages>513-517</pages><artnum>S0031182003004104</artnum><issn>0031-1820</issn><eissn>1469-8161</eissn><coden>PARAAE</coden><abstract>The technique of Random Amplification Polymorphic DNA allows fragments of the genome to be amplified by means of polymerase chain reaction (PCR) without previous knowledge of their sequences. The protozoa of the genus Leishmania present great genetic variability, making it difficult to characterize the different species. A method is developed with a single 10-mers long primer, which allows the species L. braziliensis, L. mexicana, L. infantum, L. tropica, L. chagasi, L. amazonensis and L. major to be differentiated. These products amplified by RAPD have also facilitated the design of some primers that amplify L. braziliensis DNA exclusively.</abstract><cop>Cambridge, UK</cop><pub>Cambridge University Press</pub><pmid>14700186</pmid><doi>10.1017/S0031182003004104</doi><tpages>5</tpages></addata></record> |
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| ispartof | Parasitology, 2003-12, Vol.127 (6), p.513-517, Article S0031182003004104 |
| issn | 0031-1820 1469-8161 |
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| subjects | Animals Biological and medical sciences Cloning, Molecular Deoxyribonucleic acid DNA DNA Primers DNA, Protozoan - chemistry DNA, Protozoan - genetics E coli Fundamental and applied biological sciences. Psychology General aspects General aspects and techniques. Study of several systematic groups. Models Genetic Variation Humans Invertebrates Leishmania - classification Leishmania - genetics Leishmania amazonensis Leishmania braziliensis Leishmania chagasi Leishmania infantum Leishmania mexicana Leishmania tropica Leishmaniasis - diagnosis Methods Molecular weight Nucleic Acid Hybridization PCR primer Protocol Random Amplified Polymorphic DNA Technique - methods RAPD Temperature |
| title | RAPD method useful for distinguishing Leishmania species: design of specific primers for L. braziliensis |
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