Efficiency of de Novo Centromere Formation in Human Artificial Chromosomes
In a comparative study, we show that human artificial chromosome (HAC) vectors based on α-satellite (alphoid) DNA from chromosome 17 but not the Y chromosome regularly form HACs in HT1080 human cells. We constructed four structurally similar HAC vectors, two with chromosome 17 or Y alphoid DNA (17α,...
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| Veröffentlicht in: | Genomics (San Diego, Calif.) Calif.), 2002-03, Vol.79 (3), p.297-304 |
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| container_title | Genomics (San Diego, Calif.) |
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| creator | Mejı́a, José E Alazami, Anas Willmott, Adrian Marschall, Peter Levy, Elaine Earnshaw, William C Larin, Zoia |
| description | In a comparative study, we show that human artificial chromosome (HAC) vectors based on α-satellite (alphoid) DNA from chromosome 17 but not the Y chromosome regularly form HACs in HT1080 human cells. We constructed four structurally similar HAC vectors, two with chromosome 17 or Y alphoid DNA (17α, Yα) and two with 17α or Yα and the hypoxanthine guanine phosphoribosyltransferase locus (HPRT1). The 17α HAC vectors generated artificial minichromosomes in 32–79% of the HT1080 clones screened, compared with only ∼4% for the Yα HAC vectors, indicating that Yα is inefficient at forming a de novo centromere. The 17α HAC vectors produced megabase-sized, circular HACs containing multiple copies of alphoid fragments (60–250 kb) interspersed with either vector or HPRT1 DNA.The 17α-HPRT1 HACs were less stable than those with 17α only, and these results may influence the design of new HAC gene transfer vectors. |
| doi_str_mv | 10.1006/geno.2002.6704 |
| format | Article |
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We constructed four structurally similar HAC vectors, two with chromosome 17 or Y alphoid DNA (17α, Yα) and two with 17α or Yα and the hypoxanthine guanine phosphoribosyltransferase locus (HPRT1). The 17α HAC vectors generated artificial minichromosomes in 32–79% of the HT1080 clones screened, compared with only ∼4% for the Yα HAC vectors, indicating that Yα is inefficient at forming a de novo centromere. The 17α HAC vectors produced megabase-sized, circular HACs containing multiple copies of alphoid fragments (60–250 kb) interspersed with either vector or HPRT1 DNA.The 17α-HPRT1 HACs were less stable than those with 17α only, and these results may influence the design of new HAC gene transfer vectors.</description><identifier>ISSN: 0888-7543</identifier><identifier>EISSN: 1089-8646</identifier><identifier>DOI: 10.1006/geno.2002.6704</identifier><identifier>PMID: 11863359</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Biological and medical sciences ; Cell Line ; Centromere - metabolism ; chromosome 17 ; Chromosomes, Artificial, Human - metabolism ; Chromosomes, Human, Pair 17 - genetics ; Classical genetics, quantitative genetics, hybrids ; Fundamental and applied biological sciences. Psychology ; Genetic Vectors ; Genetics of eukaryotes. Biological and molecular evolution ; HPRT gene ; Human ; Humans ; Hypoxanthine Phosphoribosyltransferase - genetics ; Molecular and cellular biology ; Y Chromosome - genetics</subject><ispartof>Genomics (San Diego, Calif.), 2002-03, Vol.79 (3), p.297-304</ispartof><rights>2002 Elsevier Science (USA)</rights><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c498t-388854169e79390a4c6c5bdd4e01521d976086e0691c5373c92ff6e958ae884a3</citedby><cites>FETCH-LOGICAL-c498t-388854169e79390a4c6c5bdd4e01521d976086e0691c5373c92ff6e958ae884a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/geno.2002.6704$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13557138$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11863359$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mejı́a, José E</creatorcontrib><creatorcontrib>Alazami, Anas</creatorcontrib><creatorcontrib>Willmott, Adrian</creatorcontrib><creatorcontrib>Marschall, Peter</creatorcontrib><creatorcontrib>Levy, Elaine</creatorcontrib><creatorcontrib>Earnshaw, William C</creatorcontrib><creatorcontrib>Larin, Zoia</creatorcontrib><title>Efficiency of de Novo Centromere Formation in Human Artificial Chromosomes</title><title>Genomics (San Diego, Calif.)</title><addtitle>Genomics</addtitle><description>In a comparative study, we show that human artificial chromosome (HAC) vectors based on α-satellite (alphoid) DNA from chromosome 17 but not the Y chromosome regularly form HACs in HT1080 human cells. We constructed four structurally similar HAC vectors, two with chromosome 17 or Y alphoid DNA (17α, Yα) and two with 17α or Yα and the hypoxanthine guanine phosphoribosyltransferase locus (HPRT1). The 17α HAC vectors generated artificial minichromosomes in 32–79% of the HT1080 clones screened, compared with only ∼4% for the Yα HAC vectors, indicating that Yα is inefficient at forming a de novo centromere. The 17α HAC vectors produced megabase-sized, circular HACs containing multiple copies of alphoid fragments (60–250 kb) interspersed with either vector or HPRT1 DNA.The 17α-HPRT1 HACs were less stable than those with 17α only, and these results may influence the design of new HAC gene transfer vectors.</description><subject>Biological and medical sciences</subject><subject>Cell Line</subject><subject>Centromere - metabolism</subject><subject>chromosome 17</subject><subject>Chromosomes, Artificial, Human - metabolism</subject><subject>Chromosomes, Human, Pair 17 - genetics</subject><subject>Classical genetics, quantitative genetics, hybrids</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic Vectors</subject><subject>Genetics of eukaryotes. Biological and molecular evolution</subject><subject>HPRT gene</subject><subject>Human</subject><subject>Humans</subject><subject>Hypoxanthine Phosphoribosyltransferase - genetics</subject><subject>Molecular and cellular biology</subject><subject>Y Chromosome - genetics</subject><issn>0888-7543</issn><issn>1089-8646</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqN0T1PHDEQgGErShQuJC1l5CZ0exl_2yU68ZEIJQ3UlvHOgtHuGuw9JP49u9xJVBGp3DwzGvkl5IjBmgHon7c45jUH4GttQH4gKwbWNVZL_ZGswFrbGCXFAflS6z0AOGH5Z3LAmNVCKLciv0-7LsWEY3ymuaMt0j_5KdMNjlPJAxakZ7kMYUp5pGmkF9shjPSkTGmZCj3d3M0s15nWr-RTF_qK3_bvIbk-O73aXDSXf89_bU4umyidnRoxH6Uk0w6NEw6CjDqqm7aVCExx1jqjwWoE7VhUwojoeNdpdMoGtFYGcUiOd3sfSn7cYp38kGrEvg8j5m31hkmjrYZ3IbOSC27_B3LDwcgZrncwllxrwc4_lDSE8uwZ-KWHX3r4pYdfeswD3_ebtzcDtm98H2AGP_Yg1Bj6roQxpvrmhFKGCTs7u3M4_-xTwuLrazVsU8E4-Tanf93wAtBtpIY</recordid><startdate>20020301</startdate><enddate>20020301</enddate><creator>Mejı́a, José E</creator><creator>Alazami, Anas</creator><creator>Willmott, Adrian</creator><creator>Marschall, Peter</creator><creator>Levy, Elaine</creator><creator>Earnshaw, William C</creator><creator>Larin, Zoia</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20020301</creationdate><title>Efficiency of de Novo Centromere Formation in Human Artificial Chromosomes</title><author>Mejı́a, José E ; Alazami, Anas ; Willmott, Adrian ; Marschall, Peter ; Levy, Elaine ; Earnshaw, William C ; Larin, Zoia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c498t-388854169e79390a4c6c5bdd4e01521d976086e0691c5373c92ff6e958ae884a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Biological and medical sciences</topic><topic>Cell Line</topic><topic>Centromere - metabolism</topic><topic>chromosome 17</topic><topic>Chromosomes, Artificial, Human - metabolism</topic><topic>Chromosomes, Human, Pair 17 - genetics</topic><topic>Classical genetics, quantitative genetics, hybrids</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic Vectors</topic><topic>Genetics of eukaryotes. Biological and molecular evolution</topic><topic>HPRT gene</topic><topic>Human</topic><topic>Humans</topic><topic>Hypoxanthine Phosphoribosyltransferase - genetics</topic><topic>Molecular and cellular biology</topic><topic>Y Chromosome - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mejı́a, José E</creatorcontrib><creatorcontrib>Alazami, Anas</creatorcontrib><creatorcontrib>Willmott, Adrian</creatorcontrib><creatorcontrib>Marschall, Peter</creatorcontrib><creatorcontrib>Levy, Elaine</creatorcontrib><creatorcontrib>Earnshaw, William C</creatorcontrib><creatorcontrib>Larin, Zoia</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Genomics (San Diego, Calif.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mejı́a, José E</au><au>Alazami, Anas</au><au>Willmott, Adrian</au><au>Marschall, Peter</au><au>Levy, Elaine</au><au>Earnshaw, William C</au><au>Larin, Zoia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Efficiency of de Novo Centromere Formation in Human Artificial Chromosomes</atitle><jtitle>Genomics (San Diego, Calif.)</jtitle><addtitle>Genomics</addtitle><date>2002-03-01</date><risdate>2002</risdate><volume>79</volume><issue>3</issue><spage>297</spage><epage>304</epage><pages>297-304</pages><issn>0888-7543</issn><eissn>1089-8646</eissn><abstract>In a comparative study, we show that human artificial chromosome (HAC) vectors based on α-satellite (alphoid) DNA from chromosome 17 but not the Y chromosome regularly form HACs in HT1080 human cells. We constructed four structurally similar HAC vectors, two with chromosome 17 or Y alphoid DNA (17α, Yα) and two with 17α or Yα and the hypoxanthine guanine phosphoribosyltransferase locus (HPRT1). The 17α HAC vectors generated artificial minichromosomes in 32–79% of the HT1080 clones screened, compared with only ∼4% for the Yα HAC vectors, indicating that Yα is inefficient at forming a de novo centromere. The 17α HAC vectors produced megabase-sized, circular HACs containing multiple copies of alphoid fragments (60–250 kb) interspersed with either vector or HPRT1 DNA.The 17α-HPRT1 HACs were less stable than those with 17α only, and these results may influence the design of new HAC gene transfer vectors.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>11863359</pmid><doi>10.1006/geno.2002.6704</doi><tpages>8</tpages></addata></record> |
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| subjects | Biological and medical sciences Cell Line Centromere - metabolism chromosome 17 Chromosomes, Artificial, Human - metabolism Chromosomes, Human, Pair 17 - genetics Classical genetics, quantitative genetics, hybrids Fundamental and applied biological sciences. Psychology Genetic Vectors Genetics of eukaryotes. Biological and molecular evolution HPRT gene Human Humans Hypoxanthine Phosphoribosyltransferase - genetics Molecular and cellular biology Y Chromosome - genetics |
| title | Efficiency of de Novo Centromere Formation in Human Artificial Chromosomes |
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