Expression of cyclooxygenase‐2 in chronic hepatitis B and the effects of anti‐viral therapy
Backgound: Cyclooxygenase‐2 may play a role in the development of hepatocellular carcinoma, but the relationship between cyclooxygenase‐2 and chronic hepatitis B is unknown. Aim: To investigate the expression and cellular localization of cyclooxygenase‐2 in chronic hepatitis B patients and the effec...
Gespeichert in:
| Veröffentlicht in: | Alimentary pharmacology & therapeutics 2002-02, Vol.16 (2), p.251-260 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 260 |
|---|---|
| container_issue | 2 |
| container_start_page | 251 |
| container_title | Alimentary pharmacology & therapeutics |
| container_volume | 16 |
| creator | Cheng, A. S. L. Chan, H. L. Y. Leung, N. W. Y. Liew, C. T. To, K. F. Lai, P. B. S. Sung, J. J. Y. |
| description | Backgound:
Cyclooxygenase‐2 may play a role in the development of hepatocellular carcinoma, but the relationship between cyclooxygenase‐2 and chronic hepatitis B is unknown.
Aim:
To investigate the expression and cellular localization of cyclooxygenase‐2 in chronic hepatitis B patients and the effects of anti‐viral therapy.
Methods:
Using immunohistochemistry, in situ hybridization, Western blot and reverse transcription polymerase chain reaction, protein and messenger RNA expression and cellular localization of cyclooxygenase‐2 in 35 chronic hepatitis B patients were assessed. Fourteen histologically normal and non‐viral‐infected livers were used as controls. The cyclooxygenase‐2 immunoreactivities of paired liver biopsies from 12 patients receiving anti‐viral therapy were compared.
Results:
Immunohistochemistry and in situ hybridization revealed that cyclooxygenase‐2 expression was confined to hepatocytes. Patients with chronic hepatitis B had significantly higher cyclooxygenase‐2 expression compared with controls. The cyclooxygenase‐2 expression of hepatitis B e antigen‐positive and ‐negative chronic hepatitis B patients was not significantly different, although the necro‐inflammatory activity of the latter group was significantly lower. Over‐expression of cyclooxygenase‐2 in patients with chronic hepatitis B was further confirmed by Western blot and reverse transcription polymerase chain reaction. Twelve hepatitis B e antigen‐positive chronic hepatitis B patients received anti‐viral therapy: lamivudine in seven and interferon in five. Despite hepatitis B e antigen seroconversion, disappearance of hepatitis B virus DNA in serum, normalization of liver enzymes and a significant reduction in necro‐inflammatory activity in all 12 patients, no significant change in cyclooxygenase‐2 expression was found.
Conclusions:
Chronic hepatitis B is associated with elevated cyclooxygenase‐2 levels in hepatocytes, and the over‐expression of this enzyme does not reflect inflammatory activity. Up‐regulation of cyclooxygenase‐2 persists after successful anti‐viral therapy. |
| doi_str_mv | 10.1046/j.1365-2036.2002.01163.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_71475784</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>71475784</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4453-166b8b7aceb4398e7eaf12376c9305f97a3343cdf47b9598c09b38ff764f5aaf3</originalsourceid><addsrcrecordid>eNqNkMFO3DAQhq2KqizQV6h8KbekduzYzoEDRbRUQqIHOFsT77jrVTYJdpZubn2EPiNP0qS7gisnjzTfP575CKGc5ZxJ9WWdc6HKrGBC5QVjRc44VyLfvSOLl8YRWbBCVVlhuDgmJymtGWNKs-IDOebcKCaZWRB7vesjphS6lnaeutE1Xbcbf2ELCZ___C1oaKlbxa4Njq6whyEMIdGvFNolHVZI0Xt0Q5rD0A5hijyFCM3ci9CPZ-S9hybhx8N7Sh6-Xd9f3WS3d99_XF3eZk7KUmRcqdrUGhzWUlQGNYLnhdDKVYKVvtIghBRu6aWuq7IyjlW1MN5rJX0J4MUpOd_P7WP3uMU02E1IDpsGWuy2yWoudamNnECzB13sUorobR_DBuJoObOzXLu2s0M7O7SzXPtfrt1N0U-HP7b1BpevwYPNCfh8ACA5aHyE1oX0yk2XKVPyibvYc79Dg-ObF7CXP-_nSvwDWOmXeQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>71475784</pqid></control><display><type>article</type><title>Expression of cyclooxygenase‐2 in chronic hepatitis B and the effects of anti‐viral therapy</title><source>MEDLINE</source><source>IngentaConnect Free/Open Access Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Wiley Online Library All Journals</source><source>Wiley Online Library (Open Access Collection)</source><creator>Cheng, A. S. L. ; Chan, H. L. Y. ; Leung, N. W. Y. ; Liew, C. T. ; To, K. F. ; Lai, P. B. S. ; Sung, J. J. Y.</creator><creatorcontrib>Cheng, A. S. L. ; Chan, H. L. Y. ; Leung, N. W. Y. ; Liew, C. T. ; To, K. F. ; Lai, P. B. S. ; Sung, J. J. Y.</creatorcontrib><description>Backgound:
Cyclooxygenase‐2 may play a role in the development of hepatocellular carcinoma, but the relationship between cyclooxygenase‐2 and chronic hepatitis B is unknown.
Aim:
To investigate the expression and cellular localization of cyclooxygenase‐2 in chronic hepatitis B patients and the effects of anti‐viral therapy.
Methods:
Using immunohistochemistry, in situ hybridization, Western blot and reverse transcription polymerase chain reaction, protein and messenger RNA expression and cellular localization of cyclooxygenase‐2 in 35 chronic hepatitis B patients were assessed. Fourteen histologically normal and non‐viral‐infected livers were used as controls. The cyclooxygenase‐2 immunoreactivities of paired liver biopsies from 12 patients receiving anti‐viral therapy were compared.
Results:
Immunohistochemistry and in situ hybridization revealed that cyclooxygenase‐2 expression was confined to hepatocytes. Patients with chronic hepatitis B had significantly higher cyclooxygenase‐2 expression compared with controls. The cyclooxygenase‐2 expression of hepatitis B e antigen‐positive and ‐negative chronic hepatitis B patients was not significantly different, although the necro‐inflammatory activity of the latter group was significantly lower. Over‐expression of cyclooxygenase‐2 in patients with chronic hepatitis B was further confirmed by Western blot and reverse transcription polymerase chain reaction. Twelve hepatitis B e antigen‐positive chronic hepatitis B patients received anti‐viral therapy: lamivudine in seven and interferon in five. Despite hepatitis B e antigen seroconversion, disappearance of hepatitis B virus DNA in serum, normalization of liver enzymes and a significant reduction in necro‐inflammatory activity in all 12 patients, no significant change in cyclooxygenase‐2 expression was found.
Conclusions:
Chronic hepatitis B is associated with elevated cyclooxygenase‐2 levels in hepatocytes, and the over‐expression of this enzyme does not reflect inflammatory activity. Up‐regulation of cyclooxygenase‐2 persists after successful anti‐viral therapy.</description><identifier>ISSN: 0269-2813</identifier><identifier>EISSN: 1365-2036</identifier><identifier>DOI: 10.1046/j.1365-2036.2002.01163.x</identifier><identifier>PMID: 11860408</identifier><language>eng</language><publisher>Oxford UK: Blackwell Science Ltd</publisher><subject>Adolescent ; Adult ; Aged ; Alanine Transaminase - blood ; Antiviral Agents - therapeutic use ; Biological and medical sciences ; Case-Control Studies ; Cyclooxygenase 2 ; Female ; Hepatitis B, Chronic - drug therapy ; Hepatitis B, Chronic - enzymology ; Hepatitis B, Chronic - pathology ; Human viral diseases ; Humans ; In Situ Hybridization ; Infectious diseases ; Isoenzymes - metabolism ; Male ; Medical sciences ; Membrane Proteins ; Middle Aged ; Prostaglandin-Endoperoxide Synthases - metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; Up-Regulation ; Viral diseases ; Viral hepatitis</subject><ispartof>Alimentary pharmacology & therapeutics, 2002-02, Vol.16 (2), p.251-260</ispartof><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4453-166b8b7aceb4398e7eaf12376c9305f97a3343cdf47b9598c09b38ff764f5aaf3</citedby><cites>FETCH-LOGICAL-c4453-166b8b7aceb4398e7eaf12376c9305f97a3343cdf47b9598c09b38ff764f5aaf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1365-2036.2002.01163.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1365-2036.2002.01163.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13986851$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11860408$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cheng, A. S. L.</creatorcontrib><creatorcontrib>Chan, H. L. Y.</creatorcontrib><creatorcontrib>Leung, N. W. Y.</creatorcontrib><creatorcontrib>Liew, C. T.</creatorcontrib><creatorcontrib>To, K. F.</creatorcontrib><creatorcontrib>Lai, P. B. S.</creatorcontrib><creatorcontrib>Sung, J. J. Y.</creatorcontrib><title>Expression of cyclooxygenase‐2 in chronic hepatitis B and the effects of anti‐viral therapy</title><title>Alimentary pharmacology & therapeutics</title><addtitle>Aliment Pharmacol Ther</addtitle><description>Backgound:
Cyclooxygenase‐2 may play a role in the development of hepatocellular carcinoma, but the relationship between cyclooxygenase‐2 and chronic hepatitis B is unknown.
Aim:
To investigate the expression and cellular localization of cyclooxygenase‐2 in chronic hepatitis B patients and the effects of anti‐viral therapy.
Methods:
Using immunohistochemistry, in situ hybridization, Western blot and reverse transcription polymerase chain reaction, protein and messenger RNA expression and cellular localization of cyclooxygenase‐2 in 35 chronic hepatitis B patients were assessed. Fourteen histologically normal and non‐viral‐infected livers were used as controls. The cyclooxygenase‐2 immunoreactivities of paired liver biopsies from 12 patients receiving anti‐viral therapy were compared.
Results:
Immunohistochemistry and in situ hybridization revealed that cyclooxygenase‐2 expression was confined to hepatocytes. Patients with chronic hepatitis B had significantly higher cyclooxygenase‐2 expression compared with controls. The cyclooxygenase‐2 expression of hepatitis B e antigen‐positive and ‐negative chronic hepatitis B patients was not significantly different, although the necro‐inflammatory activity of the latter group was significantly lower. Over‐expression of cyclooxygenase‐2 in patients with chronic hepatitis B was further confirmed by Western blot and reverse transcription polymerase chain reaction. Twelve hepatitis B e antigen‐positive chronic hepatitis B patients received anti‐viral therapy: lamivudine in seven and interferon in five. Despite hepatitis B e antigen seroconversion, disappearance of hepatitis B virus DNA in serum, normalization of liver enzymes and a significant reduction in necro‐inflammatory activity in all 12 patients, no significant change in cyclooxygenase‐2 expression was found.
Conclusions:
Chronic hepatitis B is associated with elevated cyclooxygenase‐2 levels in hepatocytes, and the over‐expression of this enzyme does not reflect inflammatory activity. Up‐regulation of cyclooxygenase‐2 persists after successful anti‐viral therapy.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Alanine Transaminase - blood</subject><subject>Antiviral Agents - therapeutic use</subject><subject>Biological and medical sciences</subject><subject>Case-Control Studies</subject><subject>Cyclooxygenase 2</subject><subject>Female</subject><subject>Hepatitis B, Chronic - drug therapy</subject><subject>Hepatitis B, Chronic - enzymology</subject><subject>Hepatitis B, Chronic - pathology</subject><subject>Human viral diseases</subject><subject>Humans</subject><subject>In Situ Hybridization</subject><subject>Infectious diseases</subject><subject>Isoenzymes - metabolism</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Membrane Proteins</subject><subject>Middle Aged</subject><subject>Prostaglandin-Endoperoxide Synthases - metabolism</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Up-Regulation</subject><subject>Viral diseases</subject><subject>Viral hepatitis</subject><issn>0269-2813</issn><issn>1365-2036</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkMFO3DAQhq2KqizQV6h8KbekduzYzoEDRbRUQqIHOFsT77jrVTYJdpZubn2EPiNP0qS7gisnjzTfP575CKGc5ZxJ9WWdc6HKrGBC5QVjRc44VyLfvSOLl8YRWbBCVVlhuDgmJymtGWNKs-IDOebcKCaZWRB7vesjphS6lnaeutE1Xbcbf2ELCZ___C1oaKlbxa4Njq6whyEMIdGvFNolHVZI0Xt0Q5rD0A5hijyFCM3ci9CPZ-S9hybhx8N7Sh6-Xd9f3WS3d99_XF3eZk7KUmRcqdrUGhzWUlQGNYLnhdDKVYKVvtIghBRu6aWuq7IyjlW1MN5rJX0J4MUpOd_P7WP3uMU02E1IDpsGWuy2yWoudamNnECzB13sUorobR_DBuJoObOzXLu2s0M7O7SzXPtfrt1N0U-HP7b1BpevwYPNCfh8ACA5aHyE1oX0yk2XKVPyibvYc79Dg-ObF7CXP-_nSvwDWOmXeQ</recordid><startdate>200202</startdate><enddate>200202</enddate><creator>Cheng, A. S. L.</creator><creator>Chan, H. L. Y.</creator><creator>Leung, N. W. Y.</creator><creator>Liew, C. T.</creator><creator>To, K. F.</creator><creator>Lai, P. B. S.</creator><creator>Sung, J. J. Y.</creator><general>Blackwell Science Ltd</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200202</creationdate><title>Expression of cyclooxygenase‐2 in chronic hepatitis B and the effects of anti‐viral therapy</title><author>Cheng, A. S. L. ; Chan, H. L. Y. ; Leung, N. W. Y. ; Liew, C. T. ; To, K. F. ; Lai, P. B. S. ; Sung, J. J. Y.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4453-166b8b7aceb4398e7eaf12376c9305f97a3343cdf47b9598c09b38ff764f5aaf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Alanine Transaminase - blood</topic><topic>Antiviral Agents - therapeutic use</topic><topic>Biological and medical sciences</topic><topic>Case-Control Studies</topic><topic>Cyclooxygenase 2</topic><topic>Female</topic><topic>Hepatitis B, Chronic - drug therapy</topic><topic>Hepatitis B, Chronic - enzymology</topic><topic>Hepatitis B, Chronic - pathology</topic><topic>Human viral diseases</topic><topic>Humans</topic><topic>In Situ Hybridization</topic><topic>Infectious diseases</topic><topic>Isoenzymes - metabolism</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Membrane Proteins</topic><topic>Middle Aged</topic><topic>Prostaglandin-Endoperoxide Synthases - metabolism</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Up-Regulation</topic><topic>Viral diseases</topic><topic>Viral hepatitis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cheng, A. S. L.</creatorcontrib><creatorcontrib>Chan, H. L. Y.</creatorcontrib><creatorcontrib>Leung, N. W. Y.</creatorcontrib><creatorcontrib>Liew, C. T.</creatorcontrib><creatorcontrib>To, K. F.</creatorcontrib><creatorcontrib>Lai, P. B. S.</creatorcontrib><creatorcontrib>Sung, J. J. Y.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Alimentary pharmacology & therapeutics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cheng, A. S. L.</au><au>Chan, H. L. Y.</au><au>Leung, N. W. Y.</au><au>Liew, C. T.</au><au>To, K. F.</au><au>Lai, P. B. S.</au><au>Sung, J. J. Y.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of cyclooxygenase‐2 in chronic hepatitis B and the effects of anti‐viral therapy</atitle><jtitle>Alimentary pharmacology & therapeutics</jtitle><addtitle>Aliment Pharmacol Ther</addtitle><date>2002-02</date><risdate>2002</risdate><volume>16</volume><issue>2</issue><spage>251</spage><epage>260</epage><pages>251-260</pages><issn>0269-2813</issn><eissn>1365-2036</eissn><abstract>Backgound:
Cyclooxygenase‐2 may play a role in the development of hepatocellular carcinoma, but the relationship between cyclooxygenase‐2 and chronic hepatitis B is unknown.
Aim:
To investigate the expression and cellular localization of cyclooxygenase‐2 in chronic hepatitis B patients and the effects of anti‐viral therapy.
Methods:
Using immunohistochemistry, in situ hybridization, Western blot and reverse transcription polymerase chain reaction, protein and messenger RNA expression and cellular localization of cyclooxygenase‐2 in 35 chronic hepatitis B patients were assessed. Fourteen histologically normal and non‐viral‐infected livers were used as controls. The cyclooxygenase‐2 immunoreactivities of paired liver biopsies from 12 patients receiving anti‐viral therapy were compared.
Results:
Immunohistochemistry and in situ hybridization revealed that cyclooxygenase‐2 expression was confined to hepatocytes. Patients with chronic hepatitis B had significantly higher cyclooxygenase‐2 expression compared with controls. The cyclooxygenase‐2 expression of hepatitis B e antigen‐positive and ‐negative chronic hepatitis B patients was not significantly different, although the necro‐inflammatory activity of the latter group was significantly lower. Over‐expression of cyclooxygenase‐2 in patients with chronic hepatitis B was further confirmed by Western blot and reverse transcription polymerase chain reaction. Twelve hepatitis B e antigen‐positive chronic hepatitis B patients received anti‐viral therapy: lamivudine in seven and interferon in five. Despite hepatitis B e antigen seroconversion, disappearance of hepatitis B virus DNA in serum, normalization of liver enzymes and a significant reduction in necro‐inflammatory activity in all 12 patients, no significant change in cyclooxygenase‐2 expression was found.
Conclusions:
Chronic hepatitis B is associated with elevated cyclooxygenase‐2 levels in hepatocytes, and the over‐expression of this enzyme does not reflect inflammatory activity. Up‐regulation of cyclooxygenase‐2 persists after successful anti‐viral therapy.</abstract><cop>Oxford UK</cop><pub>Blackwell Science Ltd</pub><pmid>11860408</pmid><doi>10.1046/j.1365-2036.2002.01163.x</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0269-2813 |
| ispartof | Alimentary pharmacology & therapeutics, 2002-02, Vol.16 (2), p.251-260 |
| issn | 0269-2813 1365-2036 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_71475784 |
| source | MEDLINE; IngentaConnect Free/Open Access Journals; EZB-FREE-00999 freely available EZB journals; Wiley Online Library All Journals; Wiley Online Library (Open Access Collection) |
| subjects | Adolescent Adult Aged Alanine Transaminase - blood Antiviral Agents - therapeutic use Biological and medical sciences Case-Control Studies Cyclooxygenase 2 Female Hepatitis B, Chronic - drug therapy Hepatitis B, Chronic - enzymology Hepatitis B, Chronic - pathology Human viral diseases Humans In Situ Hybridization Infectious diseases Isoenzymes - metabolism Male Medical sciences Membrane Proteins Middle Aged Prostaglandin-Endoperoxide Synthases - metabolism Reverse Transcriptase Polymerase Chain Reaction Up-Regulation Viral diseases Viral hepatitis |
| title | Expression of cyclooxygenase‐2 in chronic hepatitis B and the effects of anti‐viral therapy |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-28T13%3A12%3A32IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Expression%20of%20cyclooxygenase%E2%80%902%20in%20chronic%20hepatitis%20B%20and%20the%20effects%20of%20anti%E2%80%90viral%20therapy&rft.jtitle=Alimentary%20pharmacology%20&%20therapeutics&rft.au=Cheng,%20A.%20S.%20L.&rft.date=2002-02&rft.volume=16&rft.issue=2&rft.spage=251&rft.epage=260&rft.pages=251-260&rft.issn=0269-2813&rft.eissn=1365-2036&rft_id=info:doi/10.1046/j.1365-2036.2002.01163.x&rft_dat=%3Cproquest_cross%3E71475784%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=71475784&rft_id=info:pmid/11860408&rfr_iscdi=true |