Protective immune responses elicited in mice by immunization with formulations of poly(lactide-co-glycolide) microparticles
Parenteral administration of microparticle encapsulated DNA elicits immune responses to the encoded antigens. Experiments were performed to test whether the addition of certain lipophilic agents to such formulations enhanced the activity of a beta-galactosidase (β-gal) DNA vaccine. Addition of eithe...
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| Veröffentlicht in: | Vaccine 2002-02, Vol.20 (11), p.1524-1531 |
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| container_title | Vaccine |
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| creator | McKeever, U Barman, S Hao, T Chambers, P Song, S Lunsford, L Hsu, Y.-Y Roy, K Hedley, M.L |
| description | Parenteral administration of microparticle encapsulated DNA elicits immune responses to the encoded antigens. Experiments were performed to test whether the addition of certain lipophilic agents to such formulations enhanced the activity of a beta-galactosidase (β-gal) DNA vaccine. Addition of either taurocholic acid (TA) or monomethoxy polyethylene-glycol-distearoylphosphatidylehanolamine (PEG-DSPE) increased the efficiency of DNA encapsulation. Immunization of mice with encapsulated DNA formulations containing either compound significantly increased the number of antibody positive responders over that achieved with non-lipid containing particles. Moreover, responding animals demonstrated trends towards higher antibody titers and increased T cell responses. Tumor protection against the CT26·CL25 tumor cell line was demonstrated with lipid and non-lipid containing formulations. These results are the first demonstration of protection obtained by parenteral administration of PLG encapsulated DNA vaccines. |
| doi_str_mv | 10.1016/S0264-410X(01)00509-6 |
| format | Article |
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Experiments were performed to test whether the addition of certain lipophilic agents to such formulations enhanced the activity of a beta-galactosidase (β-gal) DNA vaccine. Addition of either taurocholic acid (TA) or monomethoxy polyethylene-glycol-distearoylphosphatidylehanolamine (PEG-DSPE) increased the efficiency of DNA encapsulation. Immunization of mice with encapsulated DNA formulations containing either compound significantly increased the number of antibody positive responders over that achieved with non-lipid containing particles. Moreover, responding animals demonstrated trends towards higher antibody titers and increased T cell responses. Tumor protection against the CT26·CL25 tumor cell line was demonstrated with lipid and non-lipid containing formulations. These results are the first demonstration of protection obtained by parenteral administration of PLG encapsulated DNA vaccines.</description><identifier>ISSN: 0264-410X</identifier><identifier>EISSN: 1873-2518</identifier><identifier>DOI: 10.1016/S0264-410X(01)00509-6</identifier><identifier>PMID: 11858858</identifier><identifier>CODEN: VACCDE</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Animals ; Antibody Formation ; Applied microbiology ; beta-Galactosidase - genetics ; beta-Galactosidase - immunology ; Biological and medical sciences ; Cancer Vaccines - administration & dosage ; Cell Line ; DNA ; DNA, Bacterial - genetics ; Drug Delivery Systems ; Fundamental and applied biological sciences. Psychology ; Histocompatibility Antigens Class I - metabolism ; Immunization - methods ; Lac Operon ; Mice ; Mice, Inbred BALB C ; Microbiology ; Microparticle ; Microspheres ; Neoplasms, Experimental - immunology ; Neoplasms, Experimental - prevention & control ; Particle Size ; PLG ; Polyglactin 910 - administration & dosage ; T-Lymphocytes - immunology ; Tumor Cells, Cultured ; Vaccine ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) ; Vaccines, DNA - administration & dosage ; Virology</subject><ispartof>Vaccine, 2002-02, Vol.20 (11), p.1524-1531</ispartof><rights>2002 Elsevier Science Ltd</rights><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c455t-12344d6dea51b03213d0ce8ae1d9a2f65ccd3865d52ffd790363e460e697e7b53</citedby><cites>FETCH-LOGICAL-c455t-12344d6dea51b03213d0ce8ae1d9a2f65ccd3865d52ffd790363e460e697e7b53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0264410X01005096$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13513730$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11858858$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>McKeever, U</creatorcontrib><creatorcontrib>Barman, S</creatorcontrib><creatorcontrib>Hao, T</creatorcontrib><creatorcontrib>Chambers, P</creatorcontrib><creatorcontrib>Song, S</creatorcontrib><creatorcontrib>Lunsford, L</creatorcontrib><creatorcontrib>Hsu, Y.-Y</creatorcontrib><creatorcontrib>Roy, K</creatorcontrib><creatorcontrib>Hedley, M.L</creatorcontrib><title>Protective immune responses elicited in mice by immunization with formulations of poly(lactide-co-glycolide) microparticles</title><title>Vaccine</title><addtitle>Vaccine</addtitle><description>Parenteral administration of microparticle encapsulated DNA elicits immune responses to the encoded antigens. Experiments were performed to test whether the addition of certain lipophilic agents to such formulations enhanced the activity of a beta-galactosidase (β-gal) DNA vaccine. Addition of either taurocholic acid (TA) or monomethoxy polyethylene-glycol-distearoylphosphatidylehanolamine (PEG-DSPE) increased the efficiency of DNA encapsulation. Immunization of mice with encapsulated DNA formulations containing either compound significantly increased the number of antibody positive responders over that achieved with non-lipid containing particles. Moreover, responding animals demonstrated trends towards higher antibody titers and increased T cell responses. Tumor protection against the CT26·CL25 tumor cell line was demonstrated with lipid and non-lipid containing formulations. These results are the first demonstration of protection obtained by parenteral administration of PLG encapsulated DNA vaccines.</description><subject>Animals</subject><subject>Antibody Formation</subject><subject>Applied microbiology</subject><subject>beta-Galactosidase - genetics</subject><subject>beta-Galactosidase - immunology</subject><subject>Biological and medical sciences</subject><subject>Cancer Vaccines - administration & dosage</subject><subject>Cell Line</subject><subject>DNA</subject><subject>DNA, Bacterial - genetics</subject><subject>Drug Delivery Systems</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Histocompatibility Antigens Class I - metabolism</subject><subject>Immunization - methods</subject><subject>Lac Operon</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Microbiology</subject><subject>Microparticle</subject><subject>Microspheres</subject><subject>Neoplasms, Experimental - immunology</subject><subject>Neoplasms, Experimental - prevention & control</subject><subject>Particle Size</subject><subject>PLG</subject><subject>Polyglactin 910 - administration & dosage</subject><subject>T-Lymphocytes - immunology</subject><subject>Tumor Cells, Cultured</subject><subject>Vaccine</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</subject><subject>Vaccines, DNA - administration & dosage</subject><subject>Virology</subject><issn>0264-410X</issn><issn>1873-2518</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0UtrFTEUB_Agir1WP4KSjeV2MZozmWQyK5HiCwoVVHAXcpMzGslMxmSm5dovb-4Du7MQCAm_c_L4E_Ic2CtgIF9_YbVsqgbY9zWDc8YE6yr5gKxAtbyqBaiHZPWPnJAnOf9iRXHoHpMTACVUGSty-znFGe3sr5H6YVhGpAnzFMeMmWLw1s_oqB_p4C3SzfaA_B8z-zjSGz__pH1MwxL2G5nGnk4xbNfBlJ4OKxurH2FrYyiL812TFCeTZm8D5qfkUW9CxmfH-ZR8e__u68XH6vLqw6eLt5eVbYSYK6h50zjp0AjYMF4Dd8yiMgiuM3UvhbWOKymcqPvetR3jkmMjGcquxXYj-Ck5O_SdUvy9YJ714LPFEMyIccm6haYVsrkfguIKaqUKXP8fCsbaTjZ1W6g40PLynBP2ekp-MGmrgeldknqfpN7FpBnofZJalroXxyOWzYDuruoYXQEvj8Bka0KfzGh9vnNcAG85K-7NwWH54muPSWfrcbTofCrBaxf9PVf5C_6uvPI</recordid><startdate>20020222</startdate><enddate>20020222</enddate><creator>McKeever, U</creator><creator>Barman, S</creator><creator>Hao, T</creator><creator>Chambers, P</creator><creator>Song, S</creator><creator>Lunsford, L</creator><creator>Hsu, Y.-Y</creator><creator>Roy, K</creator><creator>Hedley, M.L</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20020222</creationdate><title>Protective immune responses elicited in mice by immunization with formulations of poly(lactide-co-glycolide) microparticles</title><author>McKeever, U ; Barman, S ; Hao, T ; Chambers, P ; Song, S ; Lunsford, L ; Hsu, Y.-Y ; Roy, K ; Hedley, M.L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c455t-12344d6dea51b03213d0ce8ae1d9a2f65ccd3865d52ffd790363e460e697e7b53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>Antibody Formation</topic><topic>Applied microbiology</topic><topic>beta-Galactosidase - genetics</topic><topic>beta-Galactosidase - immunology</topic><topic>Biological and medical sciences</topic><topic>Cancer Vaccines - administration & dosage</topic><topic>Cell Line</topic><topic>DNA</topic><topic>DNA, Bacterial - genetics</topic><topic>Drug Delivery Systems</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Histocompatibility Antigens Class I - metabolism</topic><topic>Immunization - methods</topic><topic>Lac Operon</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Microbiology</topic><topic>Microparticle</topic><topic>Microspheres</topic><topic>Neoplasms, Experimental - immunology</topic><topic>Neoplasms, Experimental - prevention & control</topic><topic>Particle Size</topic><topic>PLG</topic><topic>Polyglactin 910 - administration & dosage</topic><topic>T-Lymphocytes - immunology</topic><topic>Tumor Cells, Cultured</topic><topic>Vaccine</topic><topic>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</topic><topic>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</topic><topic>Vaccines, DNA - administration & dosage</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>McKeever, U</creatorcontrib><creatorcontrib>Barman, S</creatorcontrib><creatorcontrib>Hao, T</creatorcontrib><creatorcontrib>Chambers, P</creatorcontrib><creatorcontrib>Song, S</creatorcontrib><creatorcontrib>Lunsford, L</creatorcontrib><creatorcontrib>Hsu, Y.-Y</creatorcontrib><creatorcontrib>Roy, K</creatorcontrib><creatorcontrib>Hedley, M.L</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Vaccine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>McKeever, U</au><au>Barman, S</au><au>Hao, T</au><au>Chambers, P</au><au>Song, S</au><au>Lunsford, L</au><au>Hsu, Y.-Y</au><au>Roy, K</au><au>Hedley, M.L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protective immune responses elicited in mice by immunization with formulations of poly(lactide-co-glycolide) microparticles</atitle><jtitle>Vaccine</jtitle><addtitle>Vaccine</addtitle><date>2002-02-22</date><risdate>2002</risdate><volume>20</volume><issue>11</issue><spage>1524</spage><epage>1531</epage><pages>1524-1531</pages><issn>0264-410X</issn><eissn>1873-2518</eissn><coden>VACCDE</coden><abstract>Parenteral administration of microparticle encapsulated DNA elicits immune responses to the encoded antigens. Experiments were performed to test whether the addition of certain lipophilic agents to such formulations enhanced the activity of a beta-galactosidase (β-gal) DNA vaccine. Addition of either taurocholic acid (TA) or monomethoxy polyethylene-glycol-distearoylphosphatidylehanolamine (PEG-DSPE) increased the efficiency of DNA encapsulation. Immunization of mice with encapsulated DNA formulations containing either compound significantly increased the number of antibody positive responders over that achieved with non-lipid containing particles. Moreover, responding animals demonstrated trends towards higher antibody titers and increased T cell responses. Tumor protection against the CT26·CL25 tumor cell line was demonstrated with lipid and non-lipid containing formulations. These results are the first demonstration of protection obtained by parenteral administration of PLG encapsulated DNA vaccines.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>11858858</pmid><doi>10.1016/S0264-410X(01)00509-6</doi><tpages>8</tpages></addata></record> |
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| subjects | Animals Antibody Formation Applied microbiology beta-Galactosidase - genetics beta-Galactosidase - immunology Biological and medical sciences Cancer Vaccines - administration & dosage Cell Line DNA DNA, Bacterial - genetics Drug Delivery Systems Fundamental and applied biological sciences. Psychology Histocompatibility Antigens Class I - metabolism Immunization - methods Lac Operon Mice Mice, Inbred BALB C Microbiology Microparticle Microspheres Neoplasms, Experimental - immunology Neoplasms, Experimental - prevention & control Particle Size PLG Polyglactin 910 - administration & dosage T-Lymphocytes - immunology Tumor Cells, Cultured Vaccine Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) Vaccines, DNA - administration & dosage Virology |
| title | Protective immune responses elicited in mice by immunization with formulations of poly(lactide-co-glycolide) microparticles |
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