Sequence Selective Hydrolysis of Linear DNA Using Conjugates of Zr(IV) Complexes and Peptide Nucleic Acids
A series of conjugates of peptide nucleic acids (PNA) and Zr(IV) complexes was prepared. Their ability to hydrolyze DNA was tested using MALDI-TOF mass spectrometry and HPLC. The most efficient artificial restriction enzyme found, PNA conjugate with Zr(IV) complex of tris(hydroxymethyl)-aminomethane...
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| Veröffentlicht in: | Inorganic chemistry 2003-12, Vol.42 (26), p.8618-8620 |
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| container_end_page | 8620 |
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| container_issue | 26 |
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| container_title | Inorganic chemistry |
| container_volume | 42 |
| creator | Zelder, Felix H Mokhir, Andriy A Krämer, Roland |
| description | A series of conjugates of peptide nucleic acids (PNA) and Zr(IV) complexes was prepared. Their ability to hydrolyze DNA was tested using MALDI-TOF mass spectrometry and HPLC. The most efficient artificial restriction enzyme found, PNA conjugate with Zr(IV) complex of tris(hydroxymethyl)-aminomethane, cleaves DNA targets sequence selectively in close proximity to the Zr(IV) complex. It was demonstrated that cleavage products are substrates of terminal transferase. |
| doi_str_mv | 10.1021/ic034896v |
| format | Article |
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Their ability to hydrolyze DNA was tested using MALDI-TOF mass spectrometry and HPLC. The most efficient artificial restriction enzyme found, PNA conjugate with Zr(IV) complex of tris(hydroxymethyl)-aminomethane, cleaves DNA targets sequence selectively in close proximity to the Zr(IV) complex. It was demonstrated that cleavage products are substrates of terminal transferase.</description><identifier>ISSN: 0020-1669</identifier><identifier>EISSN: 1520-510X</identifier><identifier>DOI: 10.1021/ic034896v</identifier><identifier>PMID: 14686836</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Base Sequence ; DNA, Single-Stranded - chemistry ; Hydrolysis ; Organometallic Compounds - chemical synthesis ; Organometallic Compounds - chemistry ; Peptide Nucleic Acids - chemical synthesis ; Peptide Nucleic Acids - chemistry ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Substrate Specificity ; Zirconium - chemistry</subject><ispartof>Inorganic chemistry, 2003-12, Vol.42 (26), p.8618-8620</ispartof><rights>Copyright © 2003 American Chemical Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a377t-8dd8264364bcb42cd11ff0de017b0c50bed61e59e9530811aa1a8e09f03c911a3</citedby><cites>FETCH-LOGICAL-a377t-8dd8264364bcb42cd11ff0de017b0c50bed61e59e9530811aa1a8e09f03c911a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/ic034896v$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/ic034896v$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>315,781,785,2766,27080,27928,27929,56742,56792</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14686836$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zelder, Felix H</creatorcontrib><creatorcontrib>Mokhir, Andriy A</creatorcontrib><creatorcontrib>Krämer, Roland</creatorcontrib><title>Sequence Selective Hydrolysis of Linear DNA Using Conjugates of Zr(IV) Complexes and Peptide Nucleic Acids</title><title>Inorganic chemistry</title><addtitle>Inorg. Chem</addtitle><description>A series of conjugates of peptide nucleic acids (PNA) and Zr(IV) complexes was prepared. Their ability to hydrolyze DNA was tested using MALDI-TOF mass spectrometry and HPLC. The most efficient artificial restriction enzyme found, PNA conjugate with Zr(IV) complex of tris(hydroxymethyl)-aminomethane, cleaves DNA targets sequence selectively in close proximity to the Zr(IV) complex. It was demonstrated that cleavage products are substrates of terminal transferase.</description><subject>Base Sequence</subject><subject>DNA, Single-Stranded - chemistry</subject><subject>Hydrolysis</subject><subject>Organometallic Compounds - chemical synthesis</subject><subject>Organometallic Compounds - chemistry</subject><subject>Peptide Nucleic Acids - chemical synthesis</subject><subject>Peptide Nucleic Acids - chemistry</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</subject><subject>Substrate Specificity</subject><subject>Zirconium - chemistry</subject><issn>0020-1669</issn><issn>1520-510X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkM1Lw0AQxRdRbK0e_AdkL4o9VGfzsUmOpX60UKpQK-IlbHYnZUuaxN2k2P_e1Ra9eJqZNz8evEfIOYMbBh671RL8IE745oB0WejBIGTwdki6AG5nnCcdcmLtCgASP-DHpMMCHvPY512ymuNHi6VEOscCZaM3SMdbZapia7WlVU6nukRh6N1sSBdWl0s6qspVuxQN_rzfzfXkte_EdV3gp9NEqegz1o1WSGetLFBLOpRa2VNylIvC4tl-9sji4f5lNB5Mnx4no-F0IPwoagaxUrHHA58HmcwCTyrG8hwUAosykCFkqDjDMMEk9CFmTAgmYoQkB18m7vR75GrnW5vKZbNNutZWYlGIEqvWphELohAC7sD-DpSmstZgntZGr4XZpgzS72LT32Ide7E3bbM1qj9y36QDLneAkDZdVa0pXcZ_jL4AA1h-3A</recordid><startdate>20031229</startdate><enddate>20031229</enddate><creator>Zelder, Felix H</creator><creator>Mokhir, Andriy A</creator><creator>Krämer, Roland</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20031229</creationdate><title>Sequence Selective Hydrolysis of Linear DNA Using Conjugates of Zr(IV) Complexes and Peptide Nucleic Acids</title><author>Zelder, Felix H ; Mokhir, Andriy A ; Krämer, Roland</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a377t-8dd8264364bcb42cd11ff0de017b0c50bed61e59e9530811aa1a8e09f03c911a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Base Sequence</topic><topic>DNA, Single-Stranded - chemistry</topic><topic>Hydrolysis</topic><topic>Organometallic Compounds - chemical synthesis</topic><topic>Organometallic Compounds - chemistry</topic><topic>Peptide Nucleic Acids - chemical synthesis</topic><topic>Peptide Nucleic Acids - chemistry</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</topic><topic>Substrate Specificity</topic><topic>Zirconium - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zelder, Felix H</creatorcontrib><creatorcontrib>Mokhir, Andriy A</creatorcontrib><creatorcontrib>Krämer, Roland</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Inorganic chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zelder, Felix H</au><au>Mokhir, Andriy A</au><au>Krämer, Roland</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sequence Selective Hydrolysis of Linear DNA Using Conjugates of Zr(IV) Complexes and Peptide Nucleic Acids</atitle><jtitle>Inorganic chemistry</jtitle><addtitle>Inorg. Chem</addtitle><date>2003-12-29</date><risdate>2003</risdate><volume>42</volume><issue>26</issue><spage>8618</spage><epage>8620</epage><pages>8618-8620</pages><issn>0020-1669</issn><eissn>1520-510X</eissn><abstract>A series of conjugates of peptide nucleic acids (PNA) and Zr(IV) complexes was prepared. Their ability to hydrolyze DNA was tested using MALDI-TOF mass spectrometry and HPLC. The most efficient artificial restriction enzyme found, PNA conjugate with Zr(IV) complex of tris(hydroxymethyl)-aminomethane, cleaves DNA targets sequence selectively in close proximity to the Zr(IV) complex. It was demonstrated that cleavage products are substrates of terminal transferase.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>14686836</pmid><doi>10.1021/ic034896v</doi><tpages>3</tpages></addata></record> |
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| ispartof | Inorganic chemistry, 2003-12, Vol.42 (26), p.8618-8620 |
| issn | 0020-1669 1520-510X |
| language | eng |
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| source | MEDLINE; American Chemical Society Journals |
| subjects | Base Sequence DNA, Single-Stranded - chemistry Hydrolysis Organometallic Compounds - chemical synthesis Organometallic Compounds - chemistry Peptide Nucleic Acids - chemical synthesis Peptide Nucleic Acids - chemistry Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Substrate Specificity Zirconium - chemistry |
| title | Sequence Selective Hydrolysis of Linear DNA Using Conjugates of Zr(IV) Complexes and Peptide Nucleic Acids |
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