Measurement of optical trapping forces by use of the two-photon-excited fluorescence of microspheres

Measurement of optical trapping forces by use of the two-photon-excited fluorescence of microspheres

A novel technique for the calibration of laser trapping systems that utilizes two-photon-excited fluorescence of commercial dye-stained microspheres has been demonstrated. The trapping forces as well as the trapping efficiency have been measured for various liquid environments and trapping depths. T...

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Veröffentlicht in:Optics letters 2003-12, Vol.28 (23), p.2288-2290
Hauptverfasser: Kachynski, A V, Kuzmin, A N, Pudavar, H E, Kaputa, D S, Cartwright, A N, Prasad, P N
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Sprache:eng
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Fluorescence
Microspheres
Models, Theoretical
Optics and Photonics
Photons
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container_title Optics letters
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creator Kachynski, A V
Kuzmin, A N
Pudavar, H E
Kaputa, D S
Cartwright, A N
Prasad, P N
description A novel technique for the calibration of laser trapping systems that utilizes two-photon-excited fluorescence of commercial dye-stained microspheres has been demonstrated. The trapping forces as well as the trapping efficiency have been measured for various liquid environments and trapping depths. The trapping efficiency in water was found to decrease with an increase of trapping depths because of the enlargement of the trapping beam waist caused by aberrations of the optical system.
doi_str_mv 10.1364/ol.28.002288
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source MEDLINE; Optica Publishing Group Journals
subjects Fluorescence
Microspheres
Models, Theoretical
Optics and Photonics
Photons
title Measurement of optical trapping forces by use of the two-photon-excited fluorescence of microspheres
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