A quantity control mechanism regulating levels of the HapE subunit of the Hap complex in Aspergillus nidulans: no accumulation of HapE in hapC deletion mutants
The Aspergillus nidulans CCAAT-binding complex (Hap complex) consists of at least three subunits, HapB, HapC and HapE. To investigate the quantity control mechanisms of the subunits during assembly of the Hap complex, reconstitution studies with the recombinant subunits and extracts prepared from th...
Gespeichert in:
| Veröffentlicht in: | FEBS letters 2002-02, Vol.512 (1), p.227-229 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 229 |
|---|---|
| container_issue | 1 |
| container_start_page | 227 |
| container_title | FEBS letters |
| container_volume | 512 |
| creator | Kato, M. Tateyama, Y. Hayashi, K. Naruse, F. Oonishi, R. Tanoue, S. Tanaka, A. Kobayashi, T. Tsukagoshi, N. |
| description | The
Aspergillus nidulans CCAAT-binding complex (Hap complex) consists of at least three subunits, HapB, HapC and HapE. To investigate the quantity control mechanisms of the subunits during assembly of the Hap complex, reconstitution studies with the recombinant subunits and extracts prepared from the respective
hap subunit deletion mutants were carried out. Furthermore, Western blot analysis of the Hap subunits and Northern blot analysis of the
hap genes with the respective deletion mutants were also performed. From all the results together, it was suggested that the number of the HapC molecule could adjust that of the HapE molecule by forming stable heterodimers prior to assembly of the Hap complex. |
| doi_str_mv | 10.1016/S0014-5793(02)02266-4 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_71467385</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0014579302022664</els_id><sourcerecordid>18682664</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5044-5f1e2a81b4bd62b9d71c2fb73075263c2613239de115944d19a3e3d544643ffb3</originalsourceid><addsrcrecordid>eNqNkc-O0zAQxiMEYsvCI4B8QnAI-F-chAvqVl2KtBIH4Gwl9qQ1cpysHS_0afZVcdIKuC0nyzPf982Mfln2kuB3BBPx_ivGhOdFWbM3mL7FlAqR80fZilQlyxkX1eNs9UdykT0L4QdO_4rUT7MLQqqC4qpYZfdrdBsbN5npiNTgJj9Y1IM6NM6EHnnYR9tMxu2RhTuwAQ0dmg6Ads24RSG20Znpn1qK6EcLv5BxaB1G8HtjbQzIGZ1yXPiA3IAapWK_xA5u9i5ZyXBoxg3SYGFp9HFKa4Xn2ZOusQFenN_L7Pv19ttml998-fR5s77JVYF5OrIjQJuKtLzVgra1LomiXVsyXBZUMEUFYZTVGggpas41qRsGTBecC866rmWX2etT7uiH2whhkr0JCmzaGoYYZEm4KFlVPCgklagSC56ExUmo_BCCh06O3vSNP0qC5YxQLgjlzEdiKheEcva9Og-IbQ_6r-vMLAl2J8FPY-H4f6nyentFl87cwHQpz7M-nqISWrgz4GVQBpwCbTyoSerBPLDtb1AfwJA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>18682664</pqid></control><display><type>article</type><title>A quantity control mechanism regulating levels of the HapE subunit of the Hap complex in Aspergillus nidulans: no accumulation of HapE in hapC deletion mutants</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Wiley Free Content</source><source>ScienceDirect Journals (5 years ago - present)</source><source>Wiley Online Library All Journals</source><source>Alma/SFX Local Collection</source><creator>Kato, M. ; Tateyama, Y. ; Hayashi, K. ; Naruse, F. ; Oonishi, R. ; Tanoue, S. ; Tanaka, A. ; Kobayashi, T. ; Tsukagoshi, N.</creator><creatorcontrib>Kato, M. ; Tateyama, Y. ; Hayashi, K. ; Naruse, F. ; Oonishi, R. ; Tanoue, S. ; Tanaka, A. ; Kobayashi, T. ; Tsukagoshi, N.</creatorcontrib><description>The
Aspergillus nidulans CCAAT-binding complex (Hap complex) consists of at least three subunits, HapB, HapC and HapE. To investigate the quantity control mechanisms of the subunits during assembly of the Hap complex, reconstitution studies with the recombinant subunits and extracts prepared from the respective
hap subunit deletion mutants were carried out. Furthermore, Western blot analysis of the Hap subunits and Northern blot analysis of the
hap genes with the respective deletion mutants were also performed. From all the results together, it was suggested that the number of the HapC molecule could adjust that of the HapE molecule by forming stable heterodimers prior to assembly of the Hap complex.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/S0014-5793(02)02266-4</identifier><identifier>PMID: 11852085</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Aspergillus nidulans ; Aspergillus nidulans - genetics ; CCAAT-binding complex ; CCAAT-Binding Factor - biosynthesis ; CCAAT-Binding Factor - genetics ; Dimerization ; EMSA, electrophoretic mobility shift assay ; Fungal Proteins - genetics ; Gene Deletion ; Gene Expression Regulation, Fungal ; Hap complex ; Mutation ; Protein Subunits ; RNA, Messenger - isolation & purification ; Subunit assembly</subject><ispartof>FEBS letters, 2002-02, Vol.512 (1), p.227-229</ispartof><rights>2002 Federation of European Biochemical Societies</rights><rights>FEBS Letters 512 (2002) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5044-5f1e2a81b4bd62b9d71c2fb73075263c2613239de115944d19a3e3d544643ffb3</citedby><cites>FETCH-LOGICAL-c5044-5f1e2a81b4bd62b9d71c2fb73075263c2613239de115944d19a3e3d544643ffb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2FS0014-5793%2802%2902266-4$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0014-5793(02)02266-4$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,1416,1432,3548,27923,27924,45573,45574,45994,46408,46832</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11852085$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kato, M.</creatorcontrib><creatorcontrib>Tateyama, Y.</creatorcontrib><creatorcontrib>Hayashi, K.</creatorcontrib><creatorcontrib>Naruse, F.</creatorcontrib><creatorcontrib>Oonishi, R.</creatorcontrib><creatorcontrib>Tanoue, S.</creatorcontrib><creatorcontrib>Tanaka, A.</creatorcontrib><creatorcontrib>Kobayashi, T.</creatorcontrib><creatorcontrib>Tsukagoshi, N.</creatorcontrib><title>A quantity control mechanism regulating levels of the HapE subunit of the Hap complex in Aspergillus nidulans: no accumulation of HapE in hapC deletion mutants</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>The
Aspergillus nidulans CCAAT-binding complex (Hap complex) consists of at least three subunits, HapB, HapC and HapE. To investigate the quantity control mechanisms of the subunits during assembly of the Hap complex, reconstitution studies with the recombinant subunits and extracts prepared from the respective
hap subunit deletion mutants were carried out. Furthermore, Western blot analysis of the Hap subunits and Northern blot analysis of the
hap genes with the respective deletion mutants were also performed. From all the results together, it was suggested that the number of the HapC molecule could adjust that of the HapE molecule by forming stable heterodimers prior to assembly of the Hap complex.</description><subject>Aspergillus nidulans</subject><subject>Aspergillus nidulans - genetics</subject><subject>CCAAT-binding complex</subject><subject>CCAAT-Binding Factor - biosynthesis</subject><subject>CCAAT-Binding Factor - genetics</subject><subject>Dimerization</subject><subject>EMSA, electrophoretic mobility shift assay</subject><subject>Fungal Proteins - genetics</subject><subject>Gene Deletion</subject><subject>Gene Expression Regulation, Fungal</subject><subject>Hap complex</subject><subject>Mutation</subject><subject>Protein Subunits</subject><subject>RNA, Messenger - isolation & purification</subject><subject>Subunit assembly</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc-O0zAQxiMEYsvCI4B8QnAI-F-chAvqVl2KtBIH4Gwl9qQ1cpysHS_0afZVcdIKuC0nyzPf982Mfln2kuB3BBPx_ivGhOdFWbM3mL7FlAqR80fZilQlyxkX1eNs9UdykT0L4QdO_4rUT7MLQqqC4qpYZfdrdBsbN5npiNTgJj9Y1IM6NM6EHnnYR9tMxu2RhTuwAQ0dmg6Ads24RSG20Znpn1qK6EcLv5BxaB1G8HtjbQzIGZ1yXPiA3IAapWK_xA5u9i5ZyXBoxg3SYGFp9HFKa4Xn2ZOusQFenN_L7Pv19ttml998-fR5s77JVYF5OrIjQJuKtLzVgra1LomiXVsyXBZUMEUFYZTVGggpas41qRsGTBecC866rmWX2etT7uiH2whhkr0JCmzaGoYYZEm4KFlVPCgklagSC56ExUmo_BCCh06O3vSNP0qC5YxQLgjlzEdiKheEcva9Og-IbQ_6r-vMLAl2J8FPY-H4f6nyentFl87cwHQpz7M-nqISWrgz4GVQBpwCbTyoSerBPLDtb1AfwJA</recordid><startdate>20020213</startdate><enddate>20020213</enddate><creator>Kato, M.</creator><creator>Tateyama, Y.</creator><creator>Hayashi, K.</creator><creator>Naruse, F.</creator><creator>Oonishi, R.</creator><creator>Tanoue, S.</creator><creator>Tanaka, A.</creator><creator>Kobayashi, T.</creator><creator>Tsukagoshi, N.</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>20020213</creationdate><title>A quantity control mechanism regulating levels of the HapE subunit of the Hap complex in Aspergillus nidulans: no accumulation of HapE in hapC deletion mutants</title><author>Kato, M. ; Tateyama, Y. ; Hayashi, K. ; Naruse, F. ; Oonishi, R. ; Tanoue, S. ; Tanaka, A. ; Kobayashi, T. ; Tsukagoshi, N.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5044-5f1e2a81b4bd62b9d71c2fb73075263c2613239de115944d19a3e3d544643ffb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Aspergillus nidulans</topic><topic>Aspergillus nidulans - genetics</topic><topic>CCAAT-binding complex</topic><topic>CCAAT-Binding Factor - biosynthesis</topic><topic>CCAAT-Binding Factor - genetics</topic><topic>Dimerization</topic><topic>EMSA, electrophoretic mobility shift assay</topic><topic>Fungal Proteins - genetics</topic><topic>Gene Deletion</topic><topic>Gene Expression Regulation, Fungal</topic><topic>Hap complex</topic><topic>Mutation</topic><topic>Protein Subunits</topic><topic>RNA, Messenger - isolation & purification</topic><topic>Subunit assembly</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kato, M.</creatorcontrib><creatorcontrib>Tateyama, Y.</creatorcontrib><creatorcontrib>Hayashi, K.</creatorcontrib><creatorcontrib>Naruse, F.</creatorcontrib><creatorcontrib>Oonishi, R.</creatorcontrib><creatorcontrib>Tanoue, S.</creatorcontrib><creatorcontrib>Tanaka, A.</creatorcontrib><creatorcontrib>Kobayashi, T.</creatorcontrib><creatorcontrib>Tsukagoshi, N.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kato, M.</au><au>Tateyama, Y.</au><au>Hayashi, K.</au><au>Naruse, F.</au><au>Oonishi, R.</au><au>Tanoue, S.</au><au>Tanaka, A.</au><au>Kobayashi, T.</au><au>Tsukagoshi, N.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A quantity control mechanism regulating levels of the HapE subunit of the Hap complex in Aspergillus nidulans: no accumulation of HapE in hapC deletion mutants</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>2002-02-13</date><risdate>2002</risdate><volume>512</volume><issue>1</issue><spage>227</spage><epage>229</epage><pages>227-229</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>The
Aspergillus nidulans CCAAT-binding complex (Hap complex) consists of at least three subunits, HapB, HapC and HapE. To investigate the quantity control mechanisms of the subunits during assembly of the Hap complex, reconstitution studies with the recombinant subunits and extracts prepared from the respective
hap subunit deletion mutants were carried out. Furthermore, Western blot analysis of the Hap subunits and Northern blot analysis of the
hap genes with the respective deletion mutants were also performed. From all the results together, it was suggested that the number of the HapC molecule could adjust that of the HapE molecule by forming stable heterodimers prior to assembly of the Hap complex.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>11852085</pmid><doi>10.1016/S0014-5793(02)02266-4</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0014-5793 |
| ispartof | FEBS letters, 2002-02, Vol.512 (1), p.227-229 |
| issn | 0014-5793 1873-3468 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_71467385 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Wiley Free Content; ScienceDirect Journals (5 years ago - present); Wiley Online Library All Journals; Alma/SFX Local Collection |
| subjects | Aspergillus nidulans Aspergillus nidulans - genetics CCAAT-binding complex CCAAT-Binding Factor - biosynthesis CCAAT-Binding Factor - genetics Dimerization EMSA, electrophoretic mobility shift assay Fungal Proteins - genetics Gene Deletion Gene Expression Regulation, Fungal Hap complex Mutation Protein Subunits RNA, Messenger - isolation & purification Subunit assembly |
| title | A quantity control mechanism regulating levels of the HapE subunit of the Hap complex in Aspergillus nidulans: no accumulation of HapE in hapC deletion mutants |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-12T12%3A25%3A57IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20quantity%20control%20mechanism%20regulating%20levels%20of%20the%20HapE%20subunit%20of%20the%20Hap%20complex%20in%20Aspergillus%20nidulans:%20no%20accumulation%20of%20HapE%20in%20hapC%20deletion%20mutants&rft.jtitle=FEBS%20letters&rft.au=Kato,%20M.&rft.date=2002-02-13&rft.volume=512&rft.issue=1&rft.spage=227&rft.epage=229&rft.pages=227-229&rft.issn=0014-5793&rft.eissn=1873-3468&rft_id=info:doi/10.1016/S0014-5793(02)02266-4&rft_dat=%3Cproquest_cross%3E18682664%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=18682664&rft_id=info:pmid/11852085&rft_els_id=S0014579302022664&rfr_iscdi=true |