Inhibitory effects of antifungal proteins on human immunodeficiency virus type 1 reverse transcriptase, protease and integrase
A variety of antifungal proteins were isolated from seeds of leguminous plants including French bean, cowpea, field bean, mung bean, peanut and red kidney bean. They were assayed for ability to inhibit human immunodeficiency virus type 1 (HIV-1) reverse transcriptase, protease and integrase, enzymes...
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| Veröffentlicht in: | Life sciences (1973) 2002-01, Vol.70 (8), p.927-935 |
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| container_title | Life sciences (1973) |
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| creator | Ng, T.B. Au, T.K. Lam, T.L. Ye, X.Y. Wan, D.C.C. |
| description | A variety of antifungal proteins were isolated from seeds of leguminous plants including French bean, cowpea, field bean, mung bean, peanut and red kidney bean. They were assayed for ability to inhibit human immunodeficiency virus type 1 (HIV-1) reverse transcriptase, protease and integrase, enzymes essential to the life cycle of HIV-1. It was found that the cowpea β-antifungal protein had a high potency in inhibiting HIV-1 protease and HIV-1 integrase. Cowpea α-antifungal protein was potent in inhibiting HIV-1 reverse transcriptase and HIV-1 integrase. Peanut antifungal protein was characterized by a high inhibitory activity against HIV-1 integrase and an intermediate potency in inhibiting HIV-1 reverse transcriptase and HIV-1 protease. French bean thaumatin-like protein expressed low HIV-1 protease inhibitory activity and red kidney bean lectin inhibited HIV-1 integrase by only a very small extent. Antifungal proteins from the field bean and mung bean had an intermediate potency in inhibitory HIV-1 protease and integrase. However, mung bean antifungal protein was not capable of inhibiting HIV-1 reverse transcriptase. The results indicate that nearly all leguminous antifungal proteins examined were able to inhibit HIV-1 reverse transcriptase, protease and integrase to some extent. |
| doi_str_mv | 10.1016/S0024-3205(01)01458-8 |
| format | Article |
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They were assayed for ability to inhibit human immunodeficiency virus type 1 (HIV-1) reverse transcriptase, protease and integrase, enzymes essential to the life cycle of HIV-1. It was found that the cowpea β-antifungal protein had a high potency in inhibiting HIV-1 protease and HIV-1 integrase. Cowpea α-antifungal protein was potent in inhibiting HIV-1 reverse transcriptase and HIV-1 integrase. Peanut antifungal protein was characterized by a high inhibitory activity against HIV-1 integrase and an intermediate potency in inhibiting HIV-1 reverse transcriptase and HIV-1 protease. French bean thaumatin-like protein expressed low HIV-1 protease inhibitory activity and red kidney bean lectin inhibited HIV-1 integrase by only a very small extent. Antifungal proteins from the field bean and mung bean had an intermediate potency in inhibitory HIV-1 protease and integrase. However, mung bean antifungal protein was not capable of inhibiting HIV-1 reverse transcriptase. The results indicate that nearly all leguminous antifungal proteins examined were able to inhibit HIV-1 reverse transcriptase, protease and integrase to some extent.</description><identifier>ISSN: 0024-3205</identifier><identifier>EISSN: 1879-0631</identifier><identifier>DOI: 10.1016/S0024-3205(01)01458-8</identifier><identifier>PMID: 11855377</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>Amino Acid Sequence ; Anti-HIV Agents - isolation & purification ; Anti-HIV Agents - pharmacology ; Anti-HIV enzymes ; Antifungal Agents - isolation & purification ; Antifungal Agents - pharmacology ; Antifungal proteins ; Enzyme-Linked Immunosorbent Assay ; Fabaceae - chemistry ; HIV Integrase - drug effects ; HIV Protease - drug effects ; HIV Reverse Transcriptase - antagonists & inhibitors ; HIV-1 - drug effects ; Human immunodeficiency virus 1 ; Molecular Sequence Data ; Plant Proteins - isolation & purification ; Plant Proteins - pharmacology ; Seeds - chemistry</subject><ispartof>Life sciences (1973), 2002-01, Vol.70 (8), p.927-935</ispartof><rights>2002 Elsevier Science Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c458t-d7a5862d985158ae5019fe66d474a6971916244040cbfac7fc24c2b51949bad3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0024-3205(01)01458-8$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11855377$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ng, T.B.</creatorcontrib><creatorcontrib>Au, T.K.</creatorcontrib><creatorcontrib>Lam, T.L.</creatorcontrib><creatorcontrib>Ye, X.Y.</creatorcontrib><creatorcontrib>Wan, D.C.C.</creatorcontrib><title>Inhibitory effects of antifungal proteins on human immunodeficiency virus type 1 reverse transcriptase, protease and integrase</title><title>Life sciences (1973)</title><addtitle>Life Sci</addtitle><description>A variety of antifungal proteins were isolated from seeds of leguminous plants including French bean, cowpea, field bean, mung bean, peanut and red kidney bean. They were assayed for ability to inhibit human immunodeficiency virus type 1 (HIV-1) reverse transcriptase, protease and integrase, enzymes essential to the life cycle of HIV-1. It was found that the cowpea β-antifungal protein had a high potency in inhibiting HIV-1 protease and HIV-1 integrase. Cowpea α-antifungal protein was potent in inhibiting HIV-1 reverse transcriptase and HIV-1 integrase. Peanut antifungal protein was characterized by a high inhibitory activity against HIV-1 integrase and an intermediate potency in inhibiting HIV-1 reverse transcriptase and HIV-1 protease. French bean thaumatin-like protein expressed low HIV-1 protease inhibitory activity and red kidney bean lectin inhibited HIV-1 integrase by only a very small extent. Antifungal proteins from the field bean and mung bean had an intermediate potency in inhibitory HIV-1 protease and integrase. However, mung bean antifungal protein was not capable of inhibiting HIV-1 reverse transcriptase. The results indicate that nearly all leguminous antifungal proteins examined were able to inhibit HIV-1 reverse transcriptase, protease and integrase to some extent.</description><subject>Amino Acid Sequence</subject><subject>Anti-HIV Agents - isolation & purification</subject><subject>Anti-HIV Agents - pharmacology</subject><subject>Anti-HIV enzymes</subject><subject>Antifungal Agents - isolation & purification</subject><subject>Antifungal Agents - pharmacology</subject><subject>Antifungal proteins</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Fabaceae - chemistry</subject><subject>HIV Integrase - drug effects</subject><subject>HIV Protease - drug effects</subject><subject>HIV Reverse Transcriptase - antagonists & inhibitors</subject><subject>HIV-1 - drug effects</subject><subject>Human immunodeficiency virus 1</subject><subject>Molecular Sequence Data</subject><subject>Plant Proteins - isolation & purification</subject><subject>Plant Proteins - pharmacology</subject><subject>Seeds - chemistry</subject><issn>0024-3205</issn><issn>1879-0631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhi1ERZfCTwD5hEAixZP4K6cKVUArVeqB3i3HGbdGG2exnZX2wm_H-yE49uSx9cw7nvcl5B2wS2Agv_xkrOVN1zLxkcEnBlzoRr8gK9Cqb5js4CVZ_UPOyeucfzHGhFDdK3IOoIXolFqRP7fxKQyhzGlH0Xt0JdPZUxtL8Et8tGu6SXPBEOtzpE_LZCMN07TEeUQfXMDodnQb0pJp2W2QAk24xZSRlmRjdilsis34-ShTqyo90hALPqZ6e0POvF1nfHs6L8jD928P1zfN3f2P2-uvd42re5VmVFZo2Y69FiC0RcGg9yjlyBW3slfQg2w5Z5y5wVunvGu5awcBPe8HO3YX5MNRtv7i94K5mClkh-u1jTgv2SjgsgrzZ0HQrZYd34PiCLo055zQm00Kk007A8zsAzKHgMzefcPAHAIyuva9Pw1YhgnH_12nRCpwdQSw2rENmEw-uIxjSDUdM87hmRF_AWOuoiQ</recordid><startdate>20020111</startdate><enddate>20020111</enddate><creator>Ng, T.B.</creator><creator>Au, T.K.</creator><creator>Lam, T.L.</creator><creator>Ye, X.Y.</creator><creator>Wan, D.C.C.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20020111</creationdate><title>Inhibitory effects of antifungal proteins on human immunodeficiency virus type 1 reverse transcriptase, protease and integrase</title><author>Ng, T.B. ; Au, T.K. ; Lam, T.L. ; Ye, X.Y. ; Wan, D.C.C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c458t-d7a5862d985158ae5019fe66d474a6971916244040cbfac7fc24c2b51949bad3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Amino Acid Sequence</topic><topic>Anti-HIV Agents - isolation & purification</topic><topic>Anti-HIV Agents - pharmacology</topic><topic>Anti-HIV enzymes</topic><topic>Antifungal Agents - isolation & purification</topic><topic>Antifungal Agents - pharmacology</topic><topic>Antifungal proteins</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Fabaceae - chemistry</topic><topic>HIV Integrase - drug effects</topic><topic>HIV Protease - drug effects</topic><topic>HIV Reverse Transcriptase - antagonists & inhibitors</topic><topic>HIV-1 - drug effects</topic><topic>Human immunodeficiency virus 1</topic><topic>Molecular Sequence Data</topic><topic>Plant Proteins - isolation & purification</topic><topic>Plant Proteins - pharmacology</topic><topic>Seeds - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ng, T.B.</creatorcontrib><creatorcontrib>Au, T.K.</creatorcontrib><creatorcontrib>Lam, T.L.</creatorcontrib><creatorcontrib>Ye, X.Y.</creatorcontrib><creatorcontrib>Wan, D.C.C.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Life sciences (1973)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ng, T.B.</au><au>Au, T.K.</au><au>Lam, T.L.</au><au>Ye, X.Y.</au><au>Wan, D.C.C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibitory effects of antifungal proteins on human immunodeficiency virus type 1 reverse transcriptase, protease and integrase</atitle><jtitle>Life sciences (1973)</jtitle><addtitle>Life Sci</addtitle><date>2002-01-11</date><risdate>2002</risdate><volume>70</volume><issue>8</issue><spage>927</spage><epage>935</epage><pages>927-935</pages><issn>0024-3205</issn><eissn>1879-0631</eissn><abstract>A variety of antifungal proteins were isolated from seeds of leguminous plants including French bean, cowpea, field bean, mung bean, peanut and red kidney bean. They were assayed for ability to inhibit human immunodeficiency virus type 1 (HIV-1) reverse transcriptase, protease and integrase, enzymes essential to the life cycle of HIV-1. It was found that the cowpea β-antifungal protein had a high potency in inhibiting HIV-1 protease and HIV-1 integrase. Cowpea α-antifungal protein was potent in inhibiting HIV-1 reverse transcriptase and HIV-1 integrase. Peanut antifungal protein was characterized by a high inhibitory activity against HIV-1 integrase and an intermediate potency in inhibiting HIV-1 reverse transcriptase and HIV-1 protease. French bean thaumatin-like protein expressed low HIV-1 protease inhibitory activity and red kidney bean lectin inhibited HIV-1 integrase by only a very small extent. Antifungal proteins from the field bean and mung bean had an intermediate potency in inhibitory HIV-1 protease and integrase. However, mung bean antifungal protein was not capable of inhibiting HIV-1 reverse transcriptase. The results indicate that nearly all leguminous antifungal proteins examined were able to inhibit HIV-1 reverse transcriptase, protease and integrase to some extent.</abstract><cop>Netherlands</cop><pub>Elsevier Inc</pub><pmid>11855377</pmid><doi>10.1016/S0024-3205(01)01458-8</doi><tpages>9</tpages></addata></record> |
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| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Amino Acid Sequence Anti-HIV Agents - isolation & purification Anti-HIV Agents - pharmacology Anti-HIV enzymes Antifungal Agents - isolation & purification Antifungal Agents - pharmacology Antifungal proteins Enzyme-Linked Immunosorbent Assay Fabaceae - chemistry HIV Integrase - drug effects HIV Protease - drug effects HIV Reverse Transcriptase - antagonists & inhibitors HIV-1 - drug effects Human immunodeficiency virus 1 Molecular Sequence Data Plant Proteins - isolation & purification Plant Proteins - pharmacology Seeds - chemistry |
| title | Inhibitory effects of antifungal proteins on human immunodeficiency virus type 1 reverse transcriptase, protease and integrase |
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