Bone morphogenetic proteins promote development of fetal pancreas epithelial colonies containing insulin-positive cells
Extracellular signals that guide pancreas cell development are not well characterized. In an in vitro culture system of dissociated pancreas cells from the E15.5 mouse fetus we show that, in the presence of the extracellular matrix protein laminin-1, bone morphogenetic proteins (BMPs-4, -5 and -6) p...
Gespeichert in:
| Veröffentlicht in: | Journal of cell science 2002-02, Vol.115 (Pt 4), p.753-760 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 760 |
|---|---|
| container_issue | Pt 4 |
| container_start_page | 753 |
| container_title | Journal of cell science |
| container_volume | 115 |
| creator | Jiang, Fang-Xu Stanley, Edouard G Gonez, L Jorge Harrison, Leonard C |
| description | Extracellular signals that guide pancreas cell development are not well characterized. In an in vitro culture system of dissociated pancreas cells from the E15.5 mouse fetus we show that, in the presence of the extracellular matrix protein laminin-1, bone morphogenetic proteins (BMPs-4, -5 and -6) promote the development of cystic epithelial colonies. Transforming growth factor beta1 (TGF-beta1) and activin A antagonise this effect of BMP-6 and inhibit colony formation. Histological analysis revealed that the colonies are composed of E-cadherin-positive epithelial cells, which in localised areas are insulin positive. The colonies also contain occasional glucagon-positive cells, but no somatostatin- or alpha-amylase-positive cells. These findings indicate that members of the TGF-beta superfamily regulate pancreas epithelial cell development and can promote the formation of islet-like structures in vitro. |
| doi_str_mv | 10.1242/jcs.115.4.753 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_71460440</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>71460440</sourcerecordid><originalsourceid>FETCH-LOGICAL-c390t-b4f71c4d3d2b7f2ab318dc32e3557784b992acd21d8a6217f195bf1fb8c4362f3</originalsourceid><addsrcrecordid>eNpFkE1LxDAQhoMoun4cvUpO3rpmknTTHlX8ggUveg5pOtFIm9Qmq_jvzeKCp3kZHl5mHkLOgS2BS371YdMSoF7KparFHlmAVKpqQah9smCMQ9XWQhyR45Q-GGOKt-qQHAE0q5oJWJDvmxiQjnGe3uMbBsze0mmOGX1I2zCWSHv8wiFOI4ZMo6MOsxnoZIKd0SSKk8_vOPiys3GIwWMqIWTjgw9vtBRtBh-qKSaf_RdSi8OQTsmBM0PCs908Ia_3dy-3j9X6-eHp9npdWdGyXHXSKbCyFz3vlOOmE9D0VnAUda1UI7u25cb2HPrGrDgoB23dOXBdY6VYcSdOyOVfb_nlc4Mp69Gn7QUmYNwkrUCumJSsgNUfaOeY0oxOT7MfzfyjgemtaV1M62JaS11MF_5iV7zpRuz_6Z1a8QvV4n1h</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>71460440</pqid></control><display><type>article</type><title>Bone morphogenetic proteins promote development of fetal pancreas epithelial colonies containing insulin-positive cells</title><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Company of Biologists</source><creator>Jiang, Fang-Xu ; Stanley, Edouard G ; Gonez, L Jorge ; Harrison, Leonard C</creator><creatorcontrib>Jiang, Fang-Xu ; Stanley, Edouard G ; Gonez, L Jorge ; Harrison, Leonard C</creatorcontrib><description>Extracellular signals that guide pancreas cell development are not well characterized. In an in vitro culture system of dissociated pancreas cells from the E15.5 mouse fetus we show that, in the presence of the extracellular matrix protein laminin-1, bone morphogenetic proteins (BMPs-4, -5 and -6) promote the development of cystic epithelial colonies. Transforming growth factor beta1 (TGF-beta1) and activin A antagonise this effect of BMP-6 and inhibit colony formation. Histological analysis revealed that the colonies are composed of E-cadherin-positive epithelial cells, which in localised areas are insulin positive. The colonies also contain occasional glucagon-positive cells, but no somatostatin- or alpha-amylase-positive cells. These findings indicate that members of the TGF-beta superfamily regulate pancreas epithelial cell development and can promote the formation of islet-like structures in vitro.</description><identifier>ISSN: 0021-9533</identifier><identifier>EISSN: 1477-9137</identifier><identifier>DOI: 10.1242/jcs.115.4.753</identifier><identifier>PMID: 11865031</identifier><language>eng</language><publisher>England</publisher><subject>Activins - genetics ; Activins - physiology ; Animals ; Bone Morphogenetic Protein 4 ; Bone Morphogenetic Protein 5 ; Bone Morphogenetic Protein 6 ; Bone Morphogenetic Proteins - genetics ; Bone Morphogenetic Proteins - metabolism ; Bone Morphogenetic Proteins - pharmacology ; Cadherins - metabolism ; Cell Division ; Cells, Cultured ; Colony-Forming Units Assay ; Embryonic and Fetal Development ; Epithelial Cells - chemistry ; Epithelial Cells - drug effects ; Epithelial Cells - metabolism ; Gene Expression Regulation, Developmental ; Glucagon - metabolism ; Inhibin-beta Subunits - genetics ; Inhibin-beta Subunits - physiology ; Insulin - analysis ; Laminin - metabolism ; Mice ; Mice, Inbred CBA ; Pancreas - cytology ; Pancreas - embryology ; Pancreas - metabolism ; Transforming Growth Factor beta - genetics ; Transforming Growth Factor beta - physiology</subject><ispartof>Journal of cell science, 2002-02, Vol.115 (Pt 4), p.753-760</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c390t-b4f71c4d3d2b7f2ab318dc32e3557784b992acd21d8a6217f195bf1fb8c4362f3</citedby><cites>FETCH-LOGICAL-c390t-b4f71c4d3d2b7f2ab318dc32e3557784b992acd21d8a6217f195bf1fb8c4362f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,3665,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11865031$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jiang, Fang-Xu</creatorcontrib><creatorcontrib>Stanley, Edouard G</creatorcontrib><creatorcontrib>Gonez, L Jorge</creatorcontrib><creatorcontrib>Harrison, Leonard C</creatorcontrib><title>Bone morphogenetic proteins promote development of fetal pancreas epithelial colonies containing insulin-positive cells</title><title>Journal of cell science</title><addtitle>J Cell Sci</addtitle><description>Extracellular signals that guide pancreas cell development are not well characterized. In an in vitro culture system of dissociated pancreas cells from the E15.5 mouse fetus we show that, in the presence of the extracellular matrix protein laminin-1, bone morphogenetic proteins (BMPs-4, -5 and -6) promote the development of cystic epithelial colonies. Transforming growth factor beta1 (TGF-beta1) and activin A antagonise this effect of BMP-6 and inhibit colony formation. Histological analysis revealed that the colonies are composed of E-cadherin-positive epithelial cells, which in localised areas are insulin positive. The colonies also contain occasional glucagon-positive cells, but no somatostatin- or alpha-amylase-positive cells. These findings indicate that members of the TGF-beta superfamily regulate pancreas epithelial cell development and can promote the formation of islet-like structures in vitro.</description><subject>Activins - genetics</subject><subject>Activins - physiology</subject><subject>Animals</subject><subject>Bone Morphogenetic Protein 4</subject><subject>Bone Morphogenetic Protein 5</subject><subject>Bone Morphogenetic Protein 6</subject><subject>Bone Morphogenetic Proteins - genetics</subject><subject>Bone Morphogenetic Proteins - metabolism</subject><subject>Bone Morphogenetic Proteins - pharmacology</subject><subject>Cadherins - metabolism</subject><subject>Cell Division</subject><subject>Cells, Cultured</subject><subject>Colony-Forming Units Assay</subject><subject>Embryonic and Fetal Development</subject><subject>Epithelial Cells - chemistry</subject><subject>Epithelial Cells - drug effects</subject><subject>Epithelial Cells - metabolism</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Glucagon - metabolism</subject><subject>Inhibin-beta Subunits - genetics</subject><subject>Inhibin-beta Subunits - physiology</subject><subject>Insulin - analysis</subject><subject>Laminin - metabolism</subject><subject>Mice</subject><subject>Mice, Inbred CBA</subject><subject>Pancreas - cytology</subject><subject>Pancreas - embryology</subject><subject>Pancreas - metabolism</subject><subject>Transforming Growth Factor beta - genetics</subject><subject>Transforming Growth Factor beta - physiology</subject><issn>0021-9533</issn><issn>1477-9137</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkE1LxDAQhoMoun4cvUpO3rpmknTTHlX8ggUveg5pOtFIm9Qmq_jvzeKCp3kZHl5mHkLOgS2BS371YdMSoF7KparFHlmAVKpqQah9smCMQ9XWQhyR45Q-GGOKt-qQHAE0q5oJWJDvmxiQjnGe3uMbBsze0mmOGX1I2zCWSHv8wiFOI4ZMo6MOsxnoZIKd0SSKk8_vOPiys3GIwWMqIWTjgw9vtBRtBh-qKSaf_RdSi8OQTsmBM0PCs908Ia_3dy-3j9X6-eHp9npdWdGyXHXSKbCyFz3vlOOmE9D0VnAUda1UI7u25cb2HPrGrDgoB23dOXBdY6VYcSdOyOVfb_nlc4Mp69Gn7QUmYNwkrUCumJSsgNUfaOeY0oxOT7MfzfyjgemtaV1M62JaS11MF_5iV7zpRuz_6Z1a8QvV4n1h</recordid><startdate>20020215</startdate><enddate>20020215</enddate><creator>Jiang, Fang-Xu</creator><creator>Stanley, Edouard G</creator><creator>Gonez, L Jorge</creator><creator>Harrison, Leonard C</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20020215</creationdate><title>Bone morphogenetic proteins promote development of fetal pancreas epithelial colonies containing insulin-positive cells</title><author>Jiang, Fang-Xu ; Stanley, Edouard G ; Gonez, L Jorge ; Harrison, Leonard C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c390t-b4f71c4d3d2b7f2ab318dc32e3557784b992acd21d8a6217f195bf1fb8c4362f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Activins - genetics</topic><topic>Activins - physiology</topic><topic>Animals</topic><topic>Bone Morphogenetic Protein 4</topic><topic>Bone Morphogenetic Protein 5</topic><topic>Bone Morphogenetic Protein 6</topic><topic>Bone Morphogenetic Proteins - genetics</topic><topic>Bone Morphogenetic Proteins - metabolism</topic><topic>Bone Morphogenetic Proteins - pharmacology</topic><topic>Cadherins - metabolism</topic><topic>Cell Division</topic><topic>Cells, Cultured</topic><topic>Colony-Forming Units Assay</topic><topic>Embryonic and Fetal Development</topic><topic>Epithelial Cells - chemistry</topic><topic>Epithelial Cells - drug effects</topic><topic>Epithelial Cells - metabolism</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Glucagon - metabolism</topic><topic>Inhibin-beta Subunits - genetics</topic><topic>Inhibin-beta Subunits - physiology</topic><topic>Insulin - analysis</topic><topic>Laminin - metabolism</topic><topic>Mice</topic><topic>Mice, Inbred CBA</topic><topic>Pancreas - cytology</topic><topic>Pancreas - embryology</topic><topic>Pancreas - metabolism</topic><topic>Transforming Growth Factor beta - genetics</topic><topic>Transforming Growth Factor beta - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jiang, Fang-Xu</creatorcontrib><creatorcontrib>Stanley, Edouard G</creatorcontrib><creatorcontrib>Gonez, L Jorge</creatorcontrib><creatorcontrib>Harrison, Leonard C</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of cell science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jiang, Fang-Xu</au><au>Stanley, Edouard G</au><au>Gonez, L Jorge</au><au>Harrison, Leonard C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bone morphogenetic proteins promote development of fetal pancreas epithelial colonies containing insulin-positive cells</atitle><jtitle>Journal of cell science</jtitle><addtitle>J Cell Sci</addtitle><date>2002-02-15</date><risdate>2002</risdate><volume>115</volume><issue>Pt 4</issue><spage>753</spage><epage>760</epage><pages>753-760</pages><issn>0021-9533</issn><eissn>1477-9137</eissn><abstract>Extracellular signals that guide pancreas cell development are not well characterized. In an in vitro culture system of dissociated pancreas cells from the E15.5 mouse fetus we show that, in the presence of the extracellular matrix protein laminin-1, bone morphogenetic proteins (BMPs-4, -5 and -6) promote the development of cystic epithelial colonies. Transforming growth factor beta1 (TGF-beta1) and activin A antagonise this effect of BMP-6 and inhibit colony formation. Histological analysis revealed that the colonies are composed of E-cadherin-positive epithelial cells, which in localised areas are insulin positive. The colonies also contain occasional glucagon-positive cells, but no somatostatin- or alpha-amylase-positive cells. These findings indicate that members of the TGF-beta superfamily regulate pancreas epithelial cell development and can promote the formation of islet-like structures in vitro.</abstract><cop>England</cop><pmid>11865031</pmid><doi>10.1242/jcs.115.4.753</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-9533 |
| ispartof | Journal of cell science, 2002-02, Vol.115 (Pt 4), p.753-760 |
| issn | 0021-9533 1477-9137 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_71460440 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Company of Biologists |
| subjects | Activins - genetics Activins - physiology Animals Bone Morphogenetic Protein 4 Bone Morphogenetic Protein 5 Bone Morphogenetic Protein 6 Bone Morphogenetic Proteins - genetics Bone Morphogenetic Proteins - metabolism Bone Morphogenetic Proteins - pharmacology Cadherins - metabolism Cell Division Cells, Cultured Colony-Forming Units Assay Embryonic and Fetal Development Epithelial Cells - chemistry Epithelial Cells - drug effects Epithelial Cells - metabolism Gene Expression Regulation, Developmental Glucagon - metabolism Inhibin-beta Subunits - genetics Inhibin-beta Subunits - physiology Insulin - analysis Laminin - metabolism Mice Mice, Inbred CBA Pancreas - cytology Pancreas - embryology Pancreas - metabolism Transforming Growth Factor beta - genetics Transforming Growth Factor beta - physiology |
| title | Bone morphogenetic proteins promote development of fetal pancreas epithelial colonies containing insulin-positive cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-03T18%3A29%3A18IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Bone%20morphogenetic%20proteins%20promote%20development%20of%20fetal%20pancreas%20epithelial%20colonies%20containing%20insulin-positive%20cells&rft.jtitle=Journal%20of%20cell%20science&rft.au=Jiang,%20Fang-Xu&rft.date=2002-02-15&rft.volume=115&rft.issue=Pt%204&rft.spage=753&rft.epage=760&rft.pages=753-760&rft.issn=0021-9533&rft.eissn=1477-9137&rft_id=info:doi/10.1242/jcs.115.4.753&rft_dat=%3Cproquest_cross%3E71460440%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=71460440&rft_id=info:pmid/11865031&rfr_iscdi=true |