Development of a sandwich ELISA and comparison with PCR for the detection of F11 and F165 fimbriated Escherichia coli isolates from septicaemic disease in farm animals

The P fimbriae F11 and F165 that have been demonstrated on Escherichia coli septicaemic strains in poultry and calves, respectively, possess a nearly identical major subunit that demonstrates a serological cross-reaction. A polyclonal antibody-based sandwich ELISA (sELISA) that was specific for both...

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Veröffentlicht in:	Veterinary microbiology 2002-03, Vol.85 (3), p.251-257
Hauptverfasser:	Bell, C.J, Finlay, D.A, Clarke, H.J, Taylor, M.J, Ball, H.J
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Animals, Domestic Bacteremia - diagnosis Bacteremia - microbiology Bacteremia - veterinary Bacteriology Biological and medical sciences Cattle Chickens Cross Reactions ELISA Enzyme-Linked Immunosorbent Assay - methods Enzyme-Linked Immunosorbent Assay - veterinary Epidemiology Escherichia coli Escherichia coli - genetics Escherichia coli - immunology Escherichia coli - isolation & purification Escherichia coli Infections - diagnosis Escherichia coli Infections - veterinary Fimbriae Fimbriae, Bacterial - genetics Fimbriae, Bacterial - immunology Fundamental and applied biological sciences. Psychology Horses Microbiology Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains PCR Polymerase Chain Reaction - methods Polymerase Chain Reaction - veterinary Septicaemia Sheep Swine
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