Regulation of Catecholamines by Sustained and Intermittent Hypoxia in Neuroendocrine Cells and Sympathetic Neurons
ABSTRACT—Chronic intermittent hypoxia, a characteristic feature of sleep-disordered breathing, induces hypertension through augmented sympathetic nerve activity and requires the presence of functional carotid body arterial chemoreceptors. In contrast, chronic sustained hypoxia does not alter blood p...
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Veröffentlicht in: | Hypertension (Dallas, Tex. 1979) Tex. 1979), 2003-12, Vol.42 (6), p.1130-1136 |
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creator | Hui, Anna S Striet, Justin B Gudelsky, Gary Soukhova, Galia K Gozal, Evelyne Beitner-Johnson, Dana Guo, Shang-Z Sachleben, Leroy R Haycock, John W Gozal, David Czyzyk-Krzeska, Maria F |
description | ABSTRACT—Chronic intermittent hypoxia, a characteristic feature of sleep-disordered breathing, induces hypertension through augmented sympathetic nerve activity and requires the presence of functional carotid body arterial chemoreceptors. In contrast, chronic sustained hypoxia does not alter blood pressure. We therefore analyzed the biosynthetic pathways of catecholamines in peripheral nervous system structures involved in the pathogenesis of intermittent hypoxia-induced hypertension, namely, carotid bodies, superior cervical ganglia, and adrenal glands. Rats were exposed to either intermittent hypoxia (90 seconds of room air alternating with 90 seconds of 10% O2) or to sustained hypoxia (10% O2) for 1 to 30 days. Dopamine, norepinephrine, epinephrine, dihydroxyphenylacetic acid, and 5-hydroxytyptamine contents were measured by high-performance liquid chromatography. Expression of tyrosine hydroxylase and its phosphorylated forms, dopamine β-hydroxylase, phenylethanolamine N-methyltransferase, and GTP cyclohydrolase-1 were determined by Western blot analyses. Both sustained and intermittent hypoxia significantly increased dopamine and norepinephrine content in carotid bodies but not in sympathetic ganglia or adrenal glands. In carotid bodies, both types of hypoxia augmented total levels of tyrosine hydroxylase protein and its phosphorylation on serines 19, 31, 40, as well as levels of GTP cyclohydrolase-1. However, the effects of intermittent hypoxia on catecholaminergic pathways were significantly smaller and delayed than those induced by sustained hypoxia. Thus, attenuated induction of catecholaminergic phenotype by intermittent hypoxia in carotid body may play a role in development of hypertension associated with sleep-disordered breathing. The effects of both types of hypoxia on expression of catecholaminergic enzymes in superior cervical neurons and adrenal glands were transient and small. |
doi_str_mv | 10.1161/01.HYP.0000101691.12358.26 |
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In contrast, chronic sustained hypoxia does not alter blood pressure. We therefore analyzed the biosynthetic pathways of catecholamines in peripheral nervous system structures involved in the pathogenesis of intermittent hypoxia-induced hypertension, namely, carotid bodies, superior cervical ganglia, and adrenal glands. Rats were exposed to either intermittent hypoxia (90 seconds of room air alternating with 90 seconds of 10% O2) or to sustained hypoxia (10% O2) for 1 to 30 days. Dopamine, norepinephrine, epinephrine, dihydroxyphenylacetic acid, and 5-hydroxytyptamine contents were measured by high-performance liquid chromatography. Expression of tyrosine hydroxylase and its phosphorylated forms, dopamine β-hydroxylase, phenylethanolamine N-methyltransferase, and GTP cyclohydrolase-1 were determined by Western blot analyses. Both sustained and intermittent hypoxia significantly increased dopamine and norepinephrine content in carotid bodies but not in sympathetic ganglia or adrenal glands. In carotid bodies, both types of hypoxia augmented total levels of tyrosine hydroxylase protein and its phosphorylation on serines 19, 31, 40, as well as levels of GTP cyclohydrolase-1. However, the effects of intermittent hypoxia on catecholaminergic pathways were significantly smaller and delayed than those induced by sustained hypoxia. Thus, attenuated induction of catecholaminergic phenotype by intermittent hypoxia in carotid body may play a role in development of hypertension associated with sleep-disordered breathing. The effects of both types of hypoxia on expression of catecholaminergic enzymes in superior cervical neurons and adrenal glands were transient and small.</description><identifier>ISSN: 0194-911X</identifier><identifier>EISSN: 1524-4563</identifier><identifier>DOI: 10.1161/01.HYP.0000101691.12358.26</identifier><identifier>PMID: 14597643</identifier><identifier>CODEN: HPRTDN</identifier><language>eng</language><publisher>Philadelphia, PA: American Heart Association, Inc</publisher><subject>Adrenal Glands - metabolism ; Animals ; Arterial hypertension. Arterial hypotension ; Biological and medical sciences ; Blood and lymphatic vessels ; Blood Pressure ; Cardiology. Vascular system ; Carotid Body - metabolism ; Catecholamines - biosynthesis ; GTP Cyclohydrolase - metabolism ; Hypertension - etiology ; Hypoxia - complications ; Hypoxia - metabolism ; Hypoxia - physiopathology ; Male ; Medical sciences ; Neurons - metabolism ; Neurosecretory Systems - cytology ; Neurosecretory Systems - metabolism ; Rats ; Superior Cervical Ganglion - metabolism ; Sympathetic Nervous System - cytology ; Sympathetic Nervous System - metabolism</subject><ispartof>Hypertension (Dallas, Tex. 1979), 2003-12, Vol.42 (6), p.1130-1136</ispartof><rights>2003 American Heart Association, Inc.</rights><rights>2004 INIST-CNRS</rights><rights>Copyright American Heart Association, Inc. Dec 2003</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5771-bcffbacd1ab2f518f27e1d15482b67a697d4fc00f35dcf4696464bb6305e057a3</citedby><cites>FETCH-LOGICAL-c5771-bcffbacd1ab2f518f27e1d15482b67a697d4fc00f35dcf4696464bb6305e057a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3687,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15357939$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14597643$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hui, Anna S</creatorcontrib><creatorcontrib>Striet, Justin B</creatorcontrib><creatorcontrib>Gudelsky, Gary</creatorcontrib><creatorcontrib>Soukhova, Galia K</creatorcontrib><creatorcontrib>Gozal, Evelyne</creatorcontrib><creatorcontrib>Beitner-Johnson, Dana</creatorcontrib><creatorcontrib>Guo, Shang-Z</creatorcontrib><creatorcontrib>Sachleben, Leroy R</creatorcontrib><creatorcontrib>Haycock, John W</creatorcontrib><creatorcontrib>Gozal, David</creatorcontrib><creatorcontrib>Czyzyk-Krzeska, Maria F</creatorcontrib><title>Regulation of Catecholamines by Sustained and Intermittent Hypoxia in Neuroendocrine Cells and Sympathetic Neurons</title><title>Hypertension (Dallas, Tex. 1979)</title><addtitle>Hypertension</addtitle><description>ABSTRACT—Chronic intermittent hypoxia, a characteristic feature of sleep-disordered breathing, induces hypertension through augmented sympathetic nerve activity and requires the presence of functional carotid body arterial chemoreceptors. In contrast, chronic sustained hypoxia does not alter blood pressure. We therefore analyzed the biosynthetic pathways of catecholamines in peripheral nervous system structures involved in the pathogenesis of intermittent hypoxia-induced hypertension, namely, carotid bodies, superior cervical ganglia, and adrenal glands. Rats were exposed to either intermittent hypoxia (90 seconds of room air alternating with 90 seconds of 10% O2) or to sustained hypoxia (10% O2) for 1 to 30 days. Dopamine, norepinephrine, epinephrine, dihydroxyphenylacetic acid, and 5-hydroxytyptamine contents were measured by high-performance liquid chromatography. Expression of tyrosine hydroxylase and its phosphorylated forms, dopamine β-hydroxylase, phenylethanolamine N-methyltransferase, and GTP cyclohydrolase-1 were determined by Western blot analyses. Both sustained and intermittent hypoxia significantly increased dopamine and norepinephrine content in carotid bodies but not in sympathetic ganglia or adrenal glands. In carotid bodies, both types of hypoxia augmented total levels of tyrosine hydroxylase protein and its phosphorylation on serines 19, 31, 40, as well as levels of GTP cyclohydrolase-1. However, the effects of intermittent hypoxia on catecholaminergic pathways were significantly smaller and delayed than those induced by sustained hypoxia. Thus, attenuated induction of catecholaminergic phenotype by intermittent hypoxia in carotid body may play a role in development of hypertension associated with sleep-disordered breathing. The effects of both types of hypoxia on expression of catecholaminergic enzymes in superior cervical neurons and adrenal glands were transient and small.</description><subject>Adrenal Glands - metabolism</subject><subject>Animals</subject><subject>Arterial hypertension. Arterial hypotension</subject><subject>Biological and medical sciences</subject><subject>Blood and lymphatic vessels</subject><subject>Blood Pressure</subject><subject>Cardiology. Vascular system</subject><subject>Carotid Body - metabolism</subject><subject>Catecholamines - biosynthesis</subject><subject>GTP Cyclohydrolase - metabolism</subject><subject>Hypertension - etiology</subject><subject>Hypoxia - complications</subject><subject>Hypoxia - metabolism</subject><subject>Hypoxia - physiopathology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Neurons - metabolism</subject><subject>Neurosecretory Systems - cytology</subject><subject>Neurosecretory Systems - metabolism</subject><subject>Rats</subject><subject>Superior Cervical Ganglion - metabolism</subject><subject>Sympathetic Nervous System - cytology</subject><subject>Sympathetic Nervous System - metabolism</subject><issn>0194-911X</issn><issn>1524-4563</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkV1rFDEYhQdR7Lb6FyQU9G7WvPmc8U4WdQtFxSroVchkEnfqTLImGer--2Y_YMFcJLzwnLyHc6rqGvASQMBbDMv1r69LXA5gEC0sgVDeLIl4Ui2AE1YzLujTaoGhZXUL8POiukzpvuCMMfm8ugDGWykYXVTxm_09jzoPwaPg0EpnazZh1NPgbULdDt3NKesy9Ej7Ht34bOM05Gx9RuvdNvwbNBo8-mznGKzvg4mFRSs7jukguNtNW503Ng_mCPn0onrm9Jjsy9N7Vf34-OH7al3ffvl0s3p_WxsuJdSdca7TpgfdEcehcURa6IGzhnRCatHKnjmDsaO8N46JVjDBuk5QzC3mUtOr6s3x320Mf2ebspqGZIoz7W2Yk5IlhZIHLeD1f-B9mKMv3hTBnDQltj307giZGFKK1qltHCYddwqw2teiMKhSizrXog61KCKK-NVpw9xNtj9LTz0U4PUJ0Mno0UXtzZDOHKdctrQtHDtyD2EsTaQ_4_xgo9pYPebNYTUjoqkJxhTKheu9GaCPnqmnAQ</recordid><startdate>200312</startdate><enddate>200312</enddate><creator>Hui, Anna S</creator><creator>Striet, Justin B</creator><creator>Gudelsky, Gary</creator><creator>Soukhova, Galia K</creator><creator>Gozal, Evelyne</creator><creator>Beitner-Johnson, Dana</creator><creator>Guo, Shang-Z</creator><creator>Sachleben, Leroy R</creator><creator>Haycock, John W</creator><creator>Gozal, David</creator><creator>Czyzyk-Krzeska, Maria F</creator><general>American Heart Association, Inc</general><general>Lippincott</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>200312</creationdate><title>Regulation of Catecholamines by Sustained and Intermittent Hypoxia in Neuroendocrine Cells and Sympathetic Neurons</title><author>Hui, Anna S ; Striet, Justin B ; Gudelsky, Gary ; Soukhova, Galia K ; Gozal, Evelyne ; Beitner-Johnson, Dana ; Guo, Shang-Z ; Sachleben, Leroy R ; Haycock, John W ; Gozal, David ; Czyzyk-Krzeska, Maria F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5771-bcffbacd1ab2f518f27e1d15482b67a697d4fc00f35dcf4696464bb6305e057a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Adrenal Glands - metabolism</topic><topic>Animals</topic><topic>Arterial hypertension. Arterial hypotension</topic><topic>Biological and medical sciences</topic><topic>Blood and lymphatic vessels</topic><topic>Blood Pressure</topic><topic>Cardiology. Vascular system</topic><topic>Carotid Body - metabolism</topic><topic>Catecholamines - biosynthesis</topic><topic>GTP Cyclohydrolase - metabolism</topic><topic>Hypertension - etiology</topic><topic>Hypoxia - complications</topic><topic>Hypoxia - metabolism</topic><topic>Hypoxia - physiopathology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Neurons - metabolism</topic><topic>Neurosecretory Systems - cytology</topic><topic>Neurosecretory Systems - metabolism</topic><topic>Rats</topic><topic>Superior Cervical Ganglion - metabolism</topic><topic>Sympathetic Nervous System - cytology</topic><topic>Sympathetic Nervous System - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hui, Anna S</creatorcontrib><creatorcontrib>Striet, Justin B</creatorcontrib><creatorcontrib>Gudelsky, Gary</creatorcontrib><creatorcontrib>Soukhova, Galia K</creatorcontrib><creatorcontrib>Gozal, Evelyne</creatorcontrib><creatorcontrib>Beitner-Johnson, Dana</creatorcontrib><creatorcontrib>Guo, Shang-Z</creatorcontrib><creatorcontrib>Sachleben, Leroy R</creatorcontrib><creatorcontrib>Haycock, John W</creatorcontrib><creatorcontrib>Gozal, David</creatorcontrib><creatorcontrib>Czyzyk-Krzeska, Maria F</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Hypertension (Dallas, Tex. 1979)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hui, Anna S</au><au>Striet, Justin B</au><au>Gudelsky, Gary</au><au>Soukhova, Galia K</au><au>Gozal, Evelyne</au><au>Beitner-Johnson, Dana</au><au>Guo, Shang-Z</au><au>Sachleben, Leroy R</au><au>Haycock, John W</au><au>Gozal, David</au><au>Czyzyk-Krzeska, Maria F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Regulation of Catecholamines by Sustained and Intermittent Hypoxia in Neuroendocrine Cells and Sympathetic Neurons</atitle><jtitle>Hypertension (Dallas, Tex. 1979)</jtitle><addtitle>Hypertension</addtitle><date>2003-12</date><risdate>2003</risdate><volume>42</volume><issue>6</issue><spage>1130</spage><epage>1136</epage><pages>1130-1136</pages><issn>0194-911X</issn><eissn>1524-4563</eissn><coden>HPRTDN</coden><abstract>ABSTRACT—Chronic intermittent hypoxia, a characteristic feature of sleep-disordered breathing, induces hypertension through augmented sympathetic nerve activity and requires the presence of functional carotid body arterial chemoreceptors. In contrast, chronic sustained hypoxia does not alter blood pressure. We therefore analyzed the biosynthetic pathways of catecholamines in peripheral nervous system structures involved in the pathogenesis of intermittent hypoxia-induced hypertension, namely, carotid bodies, superior cervical ganglia, and adrenal glands. Rats were exposed to either intermittent hypoxia (90 seconds of room air alternating with 90 seconds of 10% O2) or to sustained hypoxia (10% O2) for 1 to 30 days. Dopamine, norepinephrine, epinephrine, dihydroxyphenylacetic acid, and 5-hydroxytyptamine contents were measured by high-performance liquid chromatography. Expression of tyrosine hydroxylase and its phosphorylated forms, dopamine β-hydroxylase, phenylethanolamine N-methyltransferase, and GTP cyclohydrolase-1 were determined by Western blot analyses. Both sustained and intermittent hypoxia significantly increased dopamine and norepinephrine content in carotid bodies but not in sympathetic ganglia or adrenal glands. In carotid bodies, both types of hypoxia augmented total levels of tyrosine hydroxylase protein and its phosphorylation on serines 19, 31, 40, as well as levels of GTP cyclohydrolase-1. However, the effects of intermittent hypoxia on catecholaminergic pathways were significantly smaller and delayed than those induced by sustained hypoxia. Thus, attenuated induction of catecholaminergic phenotype by intermittent hypoxia in carotid body may play a role in development of hypertension associated with sleep-disordered breathing. The effects of both types of hypoxia on expression of catecholaminergic enzymes in superior cervical neurons and adrenal glands were transient and small.</abstract><cop>Philadelphia, PA</cop><cop>Hagerstown, MD</cop><pub>American Heart Association, Inc</pub><pmid>14597643</pmid><doi>10.1161/01.HYP.0000101691.12358.26</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Adrenal Glands - metabolism Animals Arterial hypertension. Arterial hypotension Biological and medical sciences Blood and lymphatic vessels Blood Pressure Cardiology. Vascular system Carotid Body - metabolism Catecholamines - biosynthesis GTP Cyclohydrolase - metabolism Hypertension - etiology Hypoxia - complications Hypoxia - metabolism Hypoxia - physiopathology Male Medical sciences Neurons - metabolism Neurosecretory Systems - cytology Neurosecretory Systems - metabolism Rats Superior Cervical Ganglion - metabolism Sympathetic Nervous System - cytology Sympathetic Nervous System - metabolism |
title | Regulation of Catecholamines by Sustained and Intermittent Hypoxia in Neuroendocrine Cells and Sympathetic Neurons |
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