Fluorescent Histochemical Techniques for Analysis of Intracellular Signaling
Intracellular signaling relies on the orchestrated cooperation of signaling proteins and modules, their intracellular localization, and membrane trafficking. Recently, a repertoire of fluorescence-based techniques, which significantly increases our potential for detailed studies of the involved mech...
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| Veröffentlicht in: | Journal of Histochemistry & Cytochemistry 2002-03, Vol.50 (3), p.289-303 |
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| container_end_page | 303 |
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| container_issue | 3 |
| container_start_page | 289 |
| container_title | Journal of Histochemistry & Cytochemistry |
| container_volume | 50 |
| creator | Oksvold, Morten P Skarpen, Ellen Widerberg, Jannicke Huitfeldt, Henrik S |
| description | Intracellular signaling relies on the orchestrated cooperation of signaling proteins and modules, their intracellular localization, and membrane trafficking. Recently, a repertoire of fluorescence-based techniques, which significantly increases our potential for detailed studies of the involved mechanisms, has been introduced. Microscopic techniques with increased resolution have been combined with improved techniques for detection of signaling proteins. Transfections of fluorescently tagged proteins have allowed in vivo microscopy of their trafficking and interactions with other proteins and intracellular structures. We present an overview of general signaling principles and a description of techniques based on fluorescent microscopy suited for studies of signaling mechanisms. |
| doi_str_mv | 10.1177/002215540205000301 |
| format | Article |
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We present an overview of general signaling principles and a description of techniques based on fluorescent microscopy suited for studies of signaling mechanisms.</description><subject>Animals</subject><subject>Biomarkers</subject><subject>Energy Transfer</subject><subject>Fluorescent Dyes</subject><subject>Histocytochemistry - methods</subject><subject>Humans</subject><subject>Microscopy, Fluorescence</subject><subject>Organelles - chemistry</subject><subject>Organelles - metabolism</subject><subject>Organelles - ultrastructure</subject><subject>Proteins - analysis</subject><subject>Proteins - metabolism</subject><subject>Signal Transduction</subject><subject>Transfection</subject><issn>0022-1554</issn><issn>1551-5044</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kM1OAjEUhRujEURfwIWZje5G-gudJSEiJCQuxHXT6dxCSWcG25kQ3t6ZQOLCxFWT2--ce-5B6JHgV0Km0zHGlBIhOKZYYIwZJldo2A1IKjDn12jYA2lPDNBdjHuMCedC3qIBIbJDGB2i9cK3dYBooGqSpYtNbXZQOqN9sgGzq9x3CzGxdUhmlfan6GJS22RVNUEb8L71OiSfbtv9uWp7j26s9hEeLu8IfS3eNvNluv54X81n69SwSdakMp9IChMMmhdAKRRZUVApueWFsDbnRmSQM5ZBBtOcsoyJnFiwsmAgjWSMjdDL2fcQ6j5fo0oX-zi6grqNakq4YFRmHUjPoAl1jAGsOgRX6nBSBKu-Q_W3w070dHFv8xKKX8mltA4Yn4Got6D2dRu68-P_ls9nxc5td0cXQMVSe98tIOp4PAqsmOrz_gByFIcs</recordid><startdate>20020301</startdate><enddate>20020301</enddate><creator>Oksvold, Morten P</creator><creator>Skarpen, Ellen</creator><creator>Widerberg, Jannicke</creator><creator>Huitfeldt, Henrik S</creator><general>Histochemical Soc</general><general>SAGE Publications</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20020301</creationdate><title>Fluorescent Histochemical Techniques for Analysis of Intracellular Signaling</title><author>Oksvold, Morten P ; Skarpen, Ellen ; Widerberg, Jannicke ; Huitfeldt, Henrik S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c369t-8b682e60ea4de22ed9dd2884f4d5ffb4c59eb339e9e7b23935b1fef8d3e8c8333</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>Biomarkers</topic><topic>Energy Transfer</topic><topic>Fluorescent Dyes</topic><topic>Histocytochemistry - methods</topic><topic>Humans</topic><topic>Microscopy, Fluorescence</topic><topic>Organelles - chemistry</topic><topic>Organelles - metabolism</topic><topic>Organelles - ultrastructure</topic><topic>Proteins - analysis</topic><topic>Proteins - metabolism</topic><topic>Signal Transduction</topic><topic>Transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Oksvold, Morten P</creatorcontrib><creatorcontrib>Skarpen, Ellen</creatorcontrib><creatorcontrib>Widerberg, Jannicke</creatorcontrib><creatorcontrib>Huitfeldt, Henrik S</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Histochemistry & Cytochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Oksvold, Morten P</au><au>Skarpen, Ellen</au><au>Widerberg, Jannicke</au><au>Huitfeldt, Henrik S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fluorescent Histochemical Techniques for Analysis of Intracellular Signaling</atitle><jtitle>Journal of Histochemistry & Cytochemistry</jtitle><addtitle>J Histochem Cytochem</addtitle><date>2002-03-01</date><risdate>2002</risdate><volume>50</volume><issue>3</issue><spage>289</spage><epage>303</epage><pages>289-303</pages><issn>0022-1554</issn><eissn>1551-5044</eissn><abstract>Intracellular signaling relies on the orchestrated cooperation of signaling proteins and modules, their intracellular localization, and membrane trafficking. 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| ispartof | Journal of Histochemistry & Cytochemistry, 2002-03, Vol.50 (3), p.289-303 |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; SAGE Complete; Free Full-Text Journals in Chemistry |
| subjects | Animals Biomarkers Energy Transfer Fluorescent Dyes Histocytochemistry - methods Humans Microscopy, Fluorescence Organelles - chemistry Organelles - metabolism Organelles - ultrastructure Proteins - analysis Proteins - metabolism Signal Transduction Transfection |
| title | Fluorescent Histochemical Techniques for Analysis of Intracellular Signaling |
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