An organ culture system for the study of the nucleus pulposus: description of the system and evaluation of the cells

The goal of this study was to develop a methodology to maintain intervertebral discs in organ culture, thereby preserving tissue architecture and metabolic function in a three-dimensional environment. Using a microdissection technique, intervertebral discs were removed from rat lumbar vertebrae. The...

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Veröffentlicht in:	Spine (Philadelphia, Pa. 1976) Pa. 1976), 2003-12, Vol.28 (24), p.2652-2658
Hauptverfasser:	Risbud, Makarand V, Izzo, Marc W, Adams, Christopher S, Arnold, William W, Hillibrand, Alan S, Vresilovic, Edward J, Vaccaro, Alexander R, Albert, Todd J, Shapiro, Irving M
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Blotting, Western Cell Survival Fluoresceins Fluorescent Dyes Intervertebral Disc - anatomy & histology Intervertebral Disc - cytology Intervertebral Disc - metabolism Male Organ Culture Techniques - methods Organic Chemicals Rats Rats, Wistar Reverse Transcriptase Polymerase Chain Reaction
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