Exopolysaccharide production by Streptococcus thermophilus SY: production and preliminary characterization of the polymer
Aims: To evaluate the effect of yeast extract (YE) concentration, temperature and pH on growth and exopolysaccharide (EPS) production in a whey‐based medium by Streptococcus thermophilus SY and to characterize the partially purified EPS. Methods and Results: Factorial experiments and empirical model...
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| Veröffentlicht in: | Journal of applied microbiology 2002-02, Vol.92 (2), p.297-306 |
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| creator | Ricciardi, A. Parente, E. Crudele, M.A. Zanetti, F. Scolari, G. Mannazzu, I. |
| description | Aims: To evaluate the effect of yeast extract (YE) concentration, temperature and pH on growth and exopolysaccharide (EPS) production in a whey‐based medium by Streptococcus thermophilus SY and to characterize the partially purified EPS.
Methods and Results: Factorial experiments and empirical model building were used to optimize fermentation conditions and the chemical composition, average molecular weight (MW) and rheological properties of aqueous dispersions of the EPS were determined. Exopolysaccharide production was growth associated and was higher (152 mg l–1) at pH 6·4 and 36°C with 4 g l–1 YE. High performance size exclusion chromatography of the partially purified EPS showed two peaks, with a weight average MW of 2 × 106 and 5 × 104, respectively. The EPS was a heteropolysaccharide, with a glucose : galactose : rhamnose ratio of 2 : 4·5 : 1. Its water dispersions had a pseudoplastic behaviour and showed a higher viscosity of xanthan solutions.
Significance and Impact of the Study: The fermentation conditions and some properties of an EPS produced by Strep. thermophilus, a dairy starter organism, were described. |
| doi_str_mv | 10.1046/j.1365-2672.2002.01487.x |
| format | Article |
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Methods and Results: Factorial experiments and empirical model building were used to optimize fermentation conditions and the chemical composition, average molecular weight (MW) and rheological properties of aqueous dispersions of the EPS were determined. Exopolysaccharide production was growth associated and was higher (152 mg l–1) at pH 6·4 and 36°C with 4 g l–1 YE. High performance size exclusion chromatography of the partially purified EPS showed two peaks, with a weight average MW of 2 × 106 and 5 × 104, respectively. The EPS was a heteropolysaccharide, with a glucose : galactose : rhamnose ratio of 2 : 4·5 : 1. Its water dispersions had a pseudoplastic behaviour and showed a higher viscosity of xanthan solutions.
Significance and Impact of the Study: The fermentation conditions and some properties of an EPS produced by Strep. thermophilus, a dairy starter organism, were described.</description><identifier>ISSN: 1364-5072</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1046/j.1365-2672.2002.01487.x</identifier><identifier>PMID: 11849358</identifier><identifier>CODEN: JAMIFK</identifier><language>eng</language><publisher>Oxford UK: Blackwell Science Ltd</publisher><subject>Biological and medical sciences ; Biopolymers - biosynthesis ; Biopolymers - chemistry ; Biotechnology ; exopolysaccharides ; Fundamental and applied biological sciences. Psychology ; Hydrogen-Ion Concentration ; Methods. Procedures. Technologies ; Microbial engineering. Fermentation and microbial culture technology ; Polysaccharides, Bacterial - biosynthesis ; Polysaccharides, Bacterial - chemistry ; Streptococcus - enzymology ; Streptococcus - growth & development ; Streptococcus thermophilus ; Temperature ; Yeasts</subject><ispartof>Journal of applied microbiology, 2002-02, Vol.92 (2), p.297-306</ispartof><rights>2003 INIST-CNRS</rights><rights>Copyright Blackwell Science Ltd. Feb 2002</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5697-db6ff66a3aa6cba278c8cda777d7bfaecfc995c6bf78e8f160c8e3683996ec523</citedby><cites>FETCH-LOGICAL-c5697-db6ff66a3aa6cba278c8cda777d7bfaecfc995c6bf78e8f160c8e3683996ec523</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1365-2672.2002.01487.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1365-2672.2002.01487.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13949772$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11849358$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ricciardi, A.</creatorcontrib><creatorcontrib>Parente, E.</creatorcontrib><creatorcontrib>Crudele, M.A.</creatorcontrib><creatorcontrib>Zanetti, F.</creatorcontrib><creatorcontrib>Scolari, G.</creatorcontrib><creatorcontrib>Mannazzu, I.</creatorcontrib><title>Exopolysaccharide production by Streptococcus thermophilus SY: production and preliminary characterization of the polymer</title><title>Journal of applied microbiology</title><addtitle>J Appl Microbiol</addtitle><description>Aims: To evaluate the effect of yeast extract (YE) concentration, temperature and pH on growth and exopolysaccharide (EPS) production in a whey‐based medium by Streptococcus thermophilus SY and to characterize the partially purified EPS.
Methods and Results: Factorial experiments and empirical model building were used to optimize fermentation conditions and the chemical composition, average molecular weight (MW) and rheological properties of aqueous dispersions of the EPS were determined. Exopolysaccharide production was growth associated and was higher (152 mg l–1) at pH 6·4 and 36°C with 4 g l–1 YE. High performance size exclusion chromatography of the partially purified EPS showed two peaks, with a weight average MW of 2 × 106 and 5 × 104, respectively. The EPS was a heteropolysaccharide, with a glucose : galactose : rhamnose ratio of 2 : 4·5 : 1. Its water dispersions had a pseudoplastic behaviour and showed a higher viscosity of xanthan solutions.
Significance and Impact of the Study: The fermentation conditions and some properties of an EPS produced by Strep. thermophilus, a dairy starter organism, were described.</description><subject>Biological and medical sciences</subject><subject>Biopolymers - biosynthesis</subject><subject>Biopolymers - chemistry</subject><subject>Biotechnology</subject><subject>exopolysaccharides</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydrogen-Ion Concentration</subject><subject>Methods. Procedures. Technologies</subject><subject>Microbial engineering. Fermentation and microbial culture technology</subject><subject>Polysaccharides, Bacterial - biosynthesis</subject><subject>Polysaccharides, Bacterial - chemistry</subject><subject>Streptococcus - enzymology</subject><subject>Streptococcus - growth & development</subject><subject>Streptococcus thermophilus</subject><subject>Temperature</subject><subject>Yeasts</subject><issn>1364-5072</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkctu1DAUhi0EomXgFVCEBLsEXxJfkFhUVWlBRSwKC1aWc2JrPEriYCfqhKcnmRlRxAZWPkf-_nP7EcoILggu-dtdQRivcsoFLSjGtMCklKLYP0Lnvz8eH-Iyr7CgZ-hZSjuMCcMVf4rOCJGlYpU8R_PVPgyhnZMB2JroG5sNMTQTjD70WT1nd2O0wxggAEwpG7c2dmHY-nZJ7r6_-xM2fbOktvWd702cs7WegdFG_9McgOBWfba262x8jp440yb74vRu0LcPV18vb_LbL9cfLy9uc6i4EnlTc-c4N8wYDrWhQoKExgghGlE7Y8GBUhXw2glppSMcg7SMS6YUt1BRtkFvjnWXUX9MNo268wls25rehilpQcoKU1X9EySSCk6WG27Qq7_AXZhivyyhKaOKUyXFAskjBDGkFK3TQ_TdchdNsF5N1Du9eqVXr_Rqoj6YqPeL9OWp_lR3tnkQnlxbgNcnwCQwrYumB58eOKZKJcS6-vsjd-9bO__3APrTxec1Yr8A0Oy7kA</recordid><startdate>200202</startdate><enddate>200202</enddate><creator>Ricciardi, A.</creator><creator>Parente, E.</creator><creator>Crudele, M.A.</creator><creator>Zanetti, F.</creator><creator>Scolari, G.</creator><creator>Mannazzu, I.</creator><general>Blackwell Science Ltd</general><general>Blackwell Science</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200202</creationdate><title>Exopolysaccharide production by Streptococcus thermophilus SY: production and preliminary characterization of the polymer</title><author>Ricciardi, A. ; Parente, E. ; Crudele, M.A. ; Zanetti, F. ; Scolari, G. ; Mannazzu, I.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5697-db6ff66a3aa6cba278c8cda777d7bfaecfc995c6bf78e8f160c8e3683996ec523</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Biological and medical sciences</topic><topic>Biopolymers - biosynthesis</topic><topic>Biopolymers - chemistry</topic><topic>Biotechnology</topic><topic>exopolysaccharides</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hydrogen-Ion Concentration</topic><topic>Methods. Procedures. Technologies</topic><topic>Microbial engineering. Fermentation and microbial culture technology</topic><topic>Polysaccharides, Bacterial - biosynthesis</topic><topic>Polysaccharides, Bacterial - chemistry</topic><topic>Streptococcus - enzymology</topic><topic>Streptococcus - growth & development</topic><topic>Streptococcus thermophilus</topic><topic>Temperature</topic><topic>Yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ricciardi, A.</creatorcontrib><creatorcontrib>Parente, E.</creatorcontrib><creatorcontrib>Crudele, M.A.</creatorcontrib><creatorcontrib>Zanetti, F.</creatorcontrib><creatorcontrib>Scolari, G.</creatorcontrib><creatorcontrib>Mannazzu, I.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ricciardi, A.</au><au>Parente, E.</au><au>Crudele, M.A.</au><au>Zanetti, F.</au><au>Scolari, G.</au><au>Mannazzu, I.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Exopolysaccharide production by Streptococcus thermophilus SY: production and preliminary characterization of the polymer</atitle><jtitle>Journal of applied microbiology</jtitle><addtitle>J Appl Microbiol</addtitle><date>2002-02</date><risdate>2002</risdate><volume>92</volume><issue>2</issue><spage>297</spage><epage>306</epage><pages>297-306</pages><issn>1364-5072</issn><eissn>1365-2672</eissn><coden>JAMIFK</coden><abstract>Aims: To evaluate the effect of yeast extract (YE) concentration, temperature and pH on growth and exopolysaccharide (EPS) production in a whey‐based medium by Streptococcus thermophilus SY and to characterize the partially purified EPS.
Methods and Results: Factorial experiments and empirical model building were used to optimize fermentation conditions and the chemical composition, average molecular weight (MW) and rheological properties of aqueous dispersions of the EPS were determined. Exopolysaccharide production was growth associated and was higher (152 mg l–1) at pH 6·4 and 36°C with 4 g l–1 YE. High performance size exclusion chromatography of the partially purified EPS showed two peaks, with a weight average MW of 2 × 106 and 5 × 104, respectively. The EPS was a heteropolysaccharide, with a glucose : galactose : rhamnose ratio of 2 : 4·5 : 1. Its water dispersions had a pseudoplastic behaviour and showed a higher viscosity of xanthan solutions.
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| ispartof | Journal of applied microbiology, 2002-02, Vol.92 (2), p.297-306 |
| issn | 1364-5072 1365-2672 |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Biological and medical sciences Biopolymers - biosynthesis Biopolymers - chemistry Biotechnology exopolysaccharides Fundamental and applied biological sciences. Psychology Hydrogen-Ion Concentration Methods. Procedures. Technologies Microbial engineering. Fermentation and microbial culture technology Polysaccharides, Bacterial - biosynthesis Polysaccharides, Bacterial - chemistry Streptococcus - enzymology Streptococcus - growth & development Streptococcus thermophilus Temperature Yeasts |
| title | Exopolysaccharide production by Streptococcus thermophilus SY: production and preliminary characterization of the polymer |
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