Involuntary leg movements affect interstitial nutrient gradients and blood flow in rat skeletal muscle
1 Wallenberg Laboratory and 2 Lundberg Laboratory for Diabetes Research, Sahlgrenska University Hospital, S-413 45 Göteborg, Sweden To evaluate the effect of passive muscle shortening and lengthening (PSL) on the transcapillary exchange of glucose, lactate, and insulin in the insulin-stimulated st...
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| Veröffentlicht in: | Journal of applied physiology (1985) 2002-03, Vol.92 (3), p.982-988 |
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| container_title | Journal of applied physiology (1985) |
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| creator | Holmang, Agneta Mimura, Kazuo Lonnroth, Peter |
| description | 1 Wallenberg Laboratory and 2 Lundberg Laboratory for
Diabetes Research, Sahlgrenska University Hospital, S-413 45 Göteborg, Sweden
To evaluate the effect of
passive muscle shortening and lengthening (PSL) on the transcapillary
exchange of glucose, lactate, and insulin in the insulin-stimulated
state, microdialysis was performed in rat quadriceps muscle. Electrical
pulsatile stimulation (0.1 ms, 0.3-0.6 V, 1 Hz) was performed on
the sciatic nerve in one leg to induce passive tension on the
quadriceps during a hyperinsulinemic-euglycemic clamp (10 mU · kg 1 · min 1 ). In the
non-insulin-stimulated (basal) state, the muscle arterial-interstitial (A-I) concentration difference of glucose was 1.6 ± 0.3 mM
( P |
| doi_str_mv | 10.1152/japplphysiol.01194.2000 |
| format | Article |
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Diabetes Research, Sahlgrenska University Hospital, S-413 45 Göteborg, Sweden
To evaluate the effect of
passive muscle shortening and lengthening (PSL) on the transcapillary
exchange of glucose, lactate, and insulin in the insulin-stimulated
state, microdialysis was performed in rat quadriceps muscle. Electrical
pulsatile stimulation (0.1 ms, 0.3-0.6 V, 1 Hz) was performed on
the sciatic nerve in one leg to induce passive tension on the
quadriceps during a hyperinsulinemic-euglycemic clamp (10 mU · kg 1 · min 1 ). In the
non-insulin-stimulated (basal) state, the muscle arterial-interstitial (A-I) concentration difference of glucose was 1.6 ± 0.3 mM
( P < 0.01). During insulin infusion, it remained
unaltered in resting muscle (1.3 ± 0.3 mM) but diminished during
PSL. In the basal state there was no A-I concentration difference of
lactate, whereas in the insulin infusion state it increased
significantly and was significantly greater in moving (2.8 ± 0.5 mM, P < 0.01) than in resting muscle (0.7 ± 0.4 mM). The A-I concentration difference of insulin was equal in resting
and moving muscle: 86 ± 7 and 100 ± 8 µU/ml,
respectively. Muscle blood flow estimated by use of radiolabeled
microspheres increased during PSL from 17 ± 4 to 34 ± 6 ml · 100 g 1 · min 1
( P < 0.05). These results confirm that diffusion over
the capillary wall is partly rate limiting for the exchange of insulin
and glucose and lactate in resting muscle. PSL, in addition to insulin
stimulation, increases blood flow and capillary permeability and, as a
result, diminishes the A-I concentration gradient of glucose but not
that of insulin or lactate.
microdialysis; arterial-interstitial concentration gradient; passive muscle shortening and lengthening</description><identifier>ISSN: 8750-7587</identifier><identifier>EISSN: 1522-1601</identifier><identifier>DOI: 10.1152/japplphysiol.01194.2000</identifier><identifier>PMID: 11842030</identifier><identifier>CODEN: JAPHEV</identifier><language>eng</language><publisher>Bethesda, MD: Am Physiological Soc</publisher><subject>Animal Nutritional Physiological Phenomena ; Animals ; Biological and medical sciences ; Electric Stimulation ; Female ; Fundamental and applied biological sciences. Psychology ; Glucose - metabolism ; Hindlimb - physiology ; Insulin - metabolism ; Insulin - pharmacology ; Lactic Acid - metabolism ; Movement - physiology ; Muscle, Skeletal - blood supply ; Muscle, Skeletal - metabolism ; Rats ; Rats, Sprague-Dawley ; Reference Values ; Regional Blood Flow - physiology ; Striated muscle. Tendons ; Vertebrates: osteoarticular system, musculoskeletal system ; Volition</subject><ispartof>Journal of applied physiology (1985), 2002-03, Vol.92 (3), p.982-988</ispartof><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c481t-3101f30e7efea53b3619a6fe16f51db48cd6897bd4701c4b3b9bfe9ca81aab2a3</citedby><cites>FETCH-LOGICAL-c481t-3101f30e7efea53b3619a6fe16f51db48cd6897bd4701c4b3b9bfe9ca81aab2a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3039,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13509633$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11842030$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Holmang, Agneta</creatorcontrib><creatorcontrib>Mimura, Kazuo</creatorcontrib><creatorcontrib>Lonnroth, Peter</creatorcontrib><title>Involuntary leg movements affect interstitial nutrient gradients and blood flow in rat skeletal muscle</title><title>Journal of applied physiology (1985)</title><addtitle>J Appl Physiol (1985)</addtitle><description>1 Wallenberg Laboratory and 2 Lundberg Laboratory for
Diabetes Research, Sahlgrenska University Hospital, S-413 45 Göteborg, Sweden
To evaluate the effect of
passive muscle shortening and lengthening (PSL) on the transcapillary
exchange of glucose, lactate, and insulin in the insulin-stimulated
state, microdialysis was performed in rat quadriceps muscle. Electrical
pulsatile stimulation (0.1 ms, 0.3-0.6 V, 1 Hz) was performed on
the sciatic nerve in one leg to induce passive tension on the
quadriceps during a hyperinsulinemic-euglycemic clamp (10 mU · kg 1 · min 1 ). In the
non-insulin-stimulated (basal) state, the muscle arterial-interstitial (A-I) concentration difference of glucose was 1.6 ± 0.3 mM
( P < 0.01). During insulin infusion, it remained
unaltered in resting muscle (1.3 ± 0.3 mM) but diminished during
PSL. In the basal state there was no A-I concentration difference of
lactate, whereas in the insulin infusion state it increased
significantly and was significantly greater in moving (2.8 ± 0.5 mM, P < 0.01) than in resting muscle (0.7 ± 0.4 mM). The A-I concentration difference of insulin was equal in resting
and moving muscle: 86 ± 7 and 100 ± 8 µU/ml,
respectively. Muscle blood flow estimated by use of radiolabeled
microspheres increased during PSL from 17 ± 4 to 34 ± 6 ml · 100 g 1 · min 1
( P < 0.05). These results confirm that diffusion over
the capillary wall is partly rate limiting for the exchange of insulin
and glucose and lactate in resting muscle. PSL, in addition to insulin
stimulation, increases blood flow and capillary permeability and, as a
result, diminishes the A-I concentration gradient of glucose but not
that of insulin or lactate.
microdialysis; arterial-interstitial concentration gradient; passive muscle shortening and lengthening</description><subject>Animal Nutritional Physiological Phenomena</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Electric Stimulation</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glucose - metabolism</subject><subject>Hindlimb - physiology</subject><subject>Insulin - metabolism</subject><subject>Insulin - pharmacology</subject><subject>Lactic Acid - metabolism</subject><subject>Movement - physiology</subject><subject>Muscle, Skeletal - blood supply</subject><subject>Muscle, Skeletal - metabolism</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Reference Values</subject><subject>Regional Blood Flow - physiology</subject><subject>Striated muscle. Tendons</subject><subject>Vertebrates: osteoarticular system, musculoskeletal system</subject><subject>Volition</subject><issn>8750-7587</issn><issn>1522-1601</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kM1u1DAURi0EotPCK4A30FUG3zh_XqKKQqVKbMracpzrGRcnDrbTdt4eDxNUNqy8uOf4kw4h74FtAery072aZzfvD9F6t2UAotqWjLEXZJOvZQENg5dk07U1K9q6a8_IeYz3jEFV1fCanAF0Vck42xBzMz14t0xJhQN1uKOjf8ARpxSpMgZ1onZKGGKyySpHpyUFm690F9RgT9g00N55P1Dj_GPGaVCJxp_oMGVjXKJ2-Ia8MspFfLu-F-TH9Ze7q2_F7fevN1efbwtddZAKDgwMZ9iiQVXznjcgVGMQGlPD0FedHppOtP1QtQx01fNe9AaFVh0o1ZeKX5CPp3_n4H8tGJMcbdTonJrQL1G2uYBoBctgewJ18DEGNHIOdswRJDB5TCz_TSz_JJbHxNl8t04s_YjDs7c2zcCHFVBRK2eCmrSNzxyvmWg4z9zlidvb3f7RBpTrmt8djutSlJJL0ZWZ5P8nrxfn7vApHZW_hpwHw38D4eSsfg</recordid><startdate>20020301</startdate><enddate>20020301</enddate><creator>Holmang, Agneta</creator><creator>Mimura, Kazuo</creator><creator>Lonnroth, Peter</creator><general>Am Physiological Soc</general><general>American Physiological Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20020301</creationdate><title>Involuntary leg movements affect interstitial nutrient gradients and blood flow in rat skeletal muscle</title><author>Holmang, Agneta ; Mimura, Kazuo ; Lonnroth, Peter</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c481t-3101f30e7efea53b3619a6fe16f51db48cd6897bd4701c4b3b9bfe9ca81aab2a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animal Nutritional Physiological Phenomena</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Electric Stimulation</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glucose - metabolism</topic><topic>Hindlimb - physiology</topic><topic>Insulin - metabolism</topic><topic>Insulin - pharmacology</topic><topic>Lactic Acid - metabolism</topic><topic>Movement - physiology</topic><topic>Muscle, Skeletal - blood supply</topic><topic>Muscle, Skeletal - metabolism</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Reference Values</topic><topic>Regional Blood Flow - physiology</topic><topic>Striated muscle. Tendons</topic><topic>Vertebrates: osteoarticular system, musculoskeletal system</topic><topic>Volition</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Holmang, Agneta</creatorcontrib><creatorcontrib>Mimura, Kazuo</creatorcontrib><creatorcontrib>Lonnroth, Peter</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of applied physiology (1985)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Holmang, Agneta</au><au>Mimura, Kazuo</au><au>Lonnroth, Peter</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Involuntary leg movements affect interstitial nutrient gradients and blood flow in rat skeletal muscle</atitle><jtitle>Journal of applied physiology (1985)</jtitle><addtitle>J Appl Physiol (1985)</addtitle><date>2002-03-01</date><risdate>2002</risdate><volume>92</volume><issue>3</issue><spage>982</spage><epage>988</epage><pages>982-988</pages><issn>8750-7587</issn><eissn>1522-1601</eissn><coden>JAPHEV</coden><abstract>1 Wallenberg Laboratory and 2 Lundberg Laboratory for
Diabetes Research, Sahlgrenska University Hospital, S-413 45 Göteborg, Sweden
To evaluate the effect of
passive muscle shortening and lengthening (PSL) on the transcapillary
exchange of glucose, lactate, and insulin in the insulin-stimulated
state, microdialysis was performed in rat quadriceps muscle. Electrical
pulsatile stimulation (0.1 ms, 0.3-0.6 V, 1 Hz) was performed on
the sciatic nerve in one leg to induce passive tension on the
quadriceps during a hyperinsulinemic-euglycemic clamp (10 mU · kg 1 · min 1 ). In the
non-insulin-stimulated (basal) state, the muscle arterial-interstitial (A-I) concentration difference of glucose was 1.6 ± 0.3 mM
( P < 0.01). During insulin infusion, it remained
unaltered in resting muscle (1.3 ± 0.3 mM) but diminished during
PSL. In the basal state there was no A-I concentration difference of
lactate, whereas in the insulin infusion state it increased
significantly and was significantly greater in moving (2.8 ± 0.5 mM, P < 0.01) than in resting muscle (0.7 ± 0.4 mM). The A-I concentration difference of insulin was equal in resting
and moving muscle: 86 ± 7 and 100 ± 8 µU/ml,
respectively. Muscle blood flow estimated by use of radiolabeled
microspheres increased during PSL from 17 ± 4 to 34 ± 6 ml · 100 g 1 · min 1
( P < 0.05). These results confirm that diffusion over
the capillary wall is partly rate limiting for the exchange of insulin
and glucose and lactate in resting muscle. PSL, in addition to insulin
stimulation, increases blood flow and capillary permeability and, as a
result, diminishes the A-I concentration gradient of glucose but not
that of insulin or lactate.
microdialysis; arterial-interstitial concentration gradient; passive muscle shortening and lengthening</abstract><cop>Bethesda, MD</cop><pub>Am Physiological Soc</pub><pmid>11842030</pmid><doi>10.1152/japplphysiol.01194.2000</doi><tpages>7</tpages></addata></record> |
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| source | MEDLINE; American Physiological Society; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Animal Nutritional Physiological Phenomena Animals Biological and medical sciences Electric Stimulation Female Fundamental and applied biological sciences. Psychology Glucose - metabolism Hindlimb - physiology Insulin - metabolism Insulin - pharmacology Lactic Acid - metabolism Movement - physiology Muscle, Skeletal - blood supply Muscle, Skeletal - metabolism Rats Rats, Sprague-Dawley Reference Values Regional Blood Flow - physiology Striated muscle. Tendons Vertebrates: osteoarticular system, musculoskeletal system Volition |
| title | Involuntary leg movements affect interstitial nutrient gradients and blood flow in rat skeletal muscle |
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