Identification of Rat EMAP, a δ-Glutamate Receptor Binding Protein
While most subtypes of glutamate receptors have been studied extensively, less is known about the δ-glutamate receptors, δ1 and δ2. Although neither forms functional channels when expressed in heterologous cells, genetic analyses have demonstrated the physiological significance of δ2. We used the cy...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2002-02, Vol.291 (1), p.85-90 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Ly, C.Dune Roche, Katherine W. Lee, Ho-Ki Wenthold, Robert J. |
| description | While most subtypes of glutamate receptors have been studied extensively, less is known about the δ-glutamate receptors, δ1 and δ2. Although neither forms functional channels when expressed in heterologous cells, genetic analyses have demonstrated the physiological significance of δ2. We used the cytosolic C-terminus of the δ2 glutamate receptor subunit in a yeast two-hybrid screen of a rat brain cDNA library to identify δ-glutamate receptor binding proteins. We isolated rat EMAP, the rat homolog of a microtubule-associated protein initially isolated and characterized in echinoderms. Rat EMAP contains 10 WD-repeats, which are domains important for mediating protein–protein interactions in a wide variety of proteins. Rat EMAP binds to δ-glutamate receptor subunits within a 50-amino-acid segment of the δ C-terminus. It is widely expressed in both brain and peripheral tissues, including high expression in brainstem and enrichment in the postsynaptic density. |
| doi_str_mv | 10.1006/bbrc.2002.6413 |
| format | Article |
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Although neither forms functional channels when expressed in heterologous cells, genetic analyses have demonstrated the physiological significance of δ2. We used the cytosolic C-terminus of the δ2 glutamate receptor subunit in a yeast two-hybrid screen of a rat brain cDNA library to identify δ-glutamate receptor binding proteins. We isolated rat EMAP, the rat homolog of a microtubule-associated protein initially isolated and characterized in echinoderms. Rat EMAP contains 10 WD-repeats, which are domains important for mediating protein–protein interactions in a wide variety of proteins. Rat EMAP binds to δ-glutamate receptor subunits within a 50-amino-acid segment of the δ C-terminus. 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Although neither forms functional channels when expressed in heterologous cells, genetic analyses have demonstrated the physiological significance of δ2. We used the cytosolic C-terminus of the δ2 glutamate receptor subunit in a yeast two-hybrid screen of a rat brain cDNA library to identify δ-glutamate receptor binding proteins. We isolated rat EMAP, the rat homolog of a microtubule-associated protein initially isolated and characterized in echinoderms. Rat EMAP contains 10 WD-repeats, which are domains important for mediating protein–protein interactions in a wide variety of proteins. Rat EMAP binds to δ-glutamate receptor subunits within a 50-amino-acid segment of the δ C-terminus. It is widely expressed in both brain and peripheral tissues, including high expression in brainstem and enrichment in the postsynaptic density.</description><subject>Animals</subject><subject>Brain Stem - metabolism</subject><subject>Cell Line</subject><subject>Cloning, Molecular</subject><subject>EMAP</subject><subject>EMAP protein</subject><subject>glutamate receptor</subject><subject>In Situ Hybridization</subject><subject>Microtubule-Associated Proteins - chemistry</subject><subject>Microtubule-Associated Proteins - genetics</subject><subject>Microtubule-Associated Proteins - metabolism</subject><subject>Molecular Sequence Data</subject><subject>Nerve Tissue Proteins - chemistry</subject><subject>Nerve Tissue Proteins - genetics</subject><subject>Nerve Tissue Proteins - metabolism</subject><subject>Organ Specificity</subject><subject>postsynaptic density</subject><subject>Protein Binding - physiology</subject><subject>Protein Subunits</subject><subject>Rats</subject><subject>Receptors, Glutamate - metabolism</subject><subject>Repetitive Sequences, Amino Acid - physiology</subject><subject>Sequence Homology, Amino Acid</subject><subject>Synapses - metabolism</subject><subject>Two-Hybrid System Techniques</subject><subject>WD-repeat</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMFKw0AQhhdRbK1ePUpOnkyd3Wyym2MttRYUS1Hwtmw2E1lpk7q7EXwvn8NnMqEFT-JhmMv3_8N8hJxTGFOA7LoonBkzADbOOE0OyJBCDjGjwA_JEDoiZjl9GZAT798AKOVZfkwGlEqW8ywbkumixDrYyhodbFNHTRWtdIhmD5PlVaSj7694vm6D3uiA0QoNbkPjohtbl7Z-jZauCWjrU3JU6bXHs_0ekefb2dP0Lr5_nC-mk_vYdKdCzHOseMlKEIJKmlRQpkIww2QhRCJlDiJjmiI3EpIEGJeYJoVM00TmMim6GZHLXe_WNe8t-qA21htcr3WNTeuVoJwLSfN_we77jDPGOnC8A41rvHdYqa2zG-0-FQXV-1W9X9X7Vb3fLnCxb26LDZa_-F5oB8gdgJ2ID4tOeWOxNlhahyaosrF_df8AwFKGgw</recordid><startdate>20020215</startdate><enddate>20020215</enddate><creator>Ly, C.Dune</creator><creator>Roche, Katherine W.</creator><creator>Lee, Ho-Ki</creator><creator>Wenthold, Robert J.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20020215</creationdate><title>Identification of Rat EMAP, a δ-Glutamate Receptor Binding Protein</title><author>Ly, C.Dune ; 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| subjects | Animals Brain Stem - metabolism Cell Line Cloning, Molecular EMAP EMAP protein glutamate receptor In Situ Hybridization Microtubule-Associated Proteins - chemistry Microtubule-Associated Proteins - genetics Microtubule-Associated Proteins - metabolism Molecular Sequence Data Nerve Tissue Proteins - chemistry Nerve Tissue Proteins - genetics Nerve Tissue Proteins - metabolism Organ Specificity postsynaptic density Protein Binding - physiology Protein Subunits Rats Receptors, Glutamate - metabolism Repetitive Sequences, Amino Acid - physiology Sequence Homology, Amino Acid Synapses - metabolism Two-Hybrid System Techniques WD-repeat |
| title | Identification of Rat EMAP, a δ-Glutamate Receptor Binding Protein |
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