Interplay of transcriptomics and proteomics
Despite the obvious attractions of parallel profiling of transcripts and proteins on a global ‘omic’ scale, there are practical and biological differences involved in their application. Transcriptomics is now a robust, high-throughput, cost-effective technology capable of simultaneously quantifying...
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| Veröffentlicht in: | Current opinion in biotechnology 2003-12, Vol.14 (6), p.647-651 |
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| container_title | Current opinion in biotechnology |
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| creator | Hegde, Priti S White, Ian R Debouck, Christine |
| description | Despite the obvious attractions of parallel profiling of transcripts and proteins on a global ‘omic’ scale, there are practical and biological differences involved in their application. Transcriptomics is now a robust, high-throughput, cost-effective technology capable of simultaneously quantifying tens of thousands of defined mRNA species in a miniaturized, automated format. Conversely, proteomic analysis is currently much more limited in breadth and depth of coverage owing to variations in protein abundance, hydrophobicity, stability, size and charge. Nevertheless, transcriptomic and proteomic data can be compared and contrasted provided the studies are carefully designed and interpreted. Differential splicing, post-translational modifications and data integration are among some of the future challenges to tackle. |
| doi_str_mv | 10.1016/j.copbio.2003.10.006 |
| format | Article |
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| ispartof | Current opinion in biotechnology, 2003-12, Vol.14 (6), p.647-651 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Biotechnology - methods Proteomics RNA, Messenger - genetics RNA, Messenger - metabolism Transcription, Genetic transcriptomics |
| title | Interplay of transcriptomics and proteomics |
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