improved protocol for Agrobacterium-mediated transformation of Antirrhinum majus L

Efficient Agrobacterium -mediated transformation of Antirrhinum majus L. was achieved via indirect shoot organogenesis from hypocotyl explants of seedlings. Stable transformants were obtained by inoculating explants with A. tumefaciens strain GV2260 harboring the binary vector pBIGFP121, which conta...

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Veröffentlicht in:	Molecular genetics and genomics : MGG 2003-12, Vol.270 (4), p.296-302
Hauptverfasser:	Cui, M.L, Handa, T, Ezura, H
Format:	Artikel
Sprache:	eng
Schlagworte:	Agrobacterium Agrobacterium tumefaciens Antirrhinum - genetics Antirrhinum majus Bacteria explants gene transfer Genes, Reporter genetic transformation Genetic Vectors GFP gene green fluorescent protein Hypocotyl hypocotyls in vitro regeneration inheritance (genetics) Inheritance Patterns Methods Microbiology Plants, Genetically Modified reporter genes Rhizobium - genetics shoots Transduction, Genetic - methods Transformation, Bacterial - genetics transgenic plants
Online-Zugang:	Volltext
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