Retroviral Delivery of the Ecdysone-Regulatable Gene Expression System
We describe a set of Moloney Murine Leukemia Virus (MoMLV)‐based replication‐defective retroviral vectors for delivery of the ecdysone‐inducible system into mammalian cells. The vector pFB‐ERV contains a tricistronic CMV expression cassette from which the ecdysone receptor proteins RXR and VgEcR are...
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| Veröffentlicht in: | Biotechnology progress 2003-11, Vol.19 (6), p.1750-1755 |
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| container_title | Biotechnology progress |
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| creator | Vaillancourt, Peter Felts, Katherine A. |
| description | We describe a set of Moloney Murine Leukemia Virus (MoMLV)‐based replication‐defective retroviral vectors for delivery of the ecdysone‐inducible system into mammalian cells. The vector pFB‐ERV contains a tricistronic CMV expression cassette from which the ecdysone receptor proteins RXR and VgEcR are expressed, with the neo‐resistance marker expressed as the third open reading frame (ORF). The inducible vector pCFB‐EGSH contains an ecdysone‐inducible expression cassette inserted between the viral LTRs in the antisense orientation relative to that for the viral promoter. Potential interference from the proviral 5′ LTR is obviated due to a SIN deletion in the 3′ LTR. When used together, induction ratios of over 1000‐fold were achieved in NIH3T3 cells using firefly luciferase as a reporter. |
| doi_str_mv | 10.1021/bp0341085 |
| format | Article |
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The vector pFB‐ERV contains a tricistronic CMV expression cassette from which the ecdysone receptor proteins RXR and VgEcR are expressed, with the neo‐resistance marker expressed as the third open reading frame (ORF). The inducible vector pCFB‐EGSH contains an ecdysone‐inducible expression cassette inserted between the viral LTRs in the antisense orientation relative to that for the viral promoter. Potential interference from the proviral 5′ LTR is obviated due to a SIN deletion in the 3′ LTR. When used together, induction ratios of over 1000‐fold were achieved in NIH3T3 cells using firefly luciferase as a reporter.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Cell Line</subject><subject>CHO Cells</subject><subject>Cricetinae</subject><subject>Cricetulus</subject><subject>Ecdysone - biosynthesis</subject><subject>Ecdysone - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation - genetics</subject><subject>Gene Targeting - methods</subject><subject>Genetic Engineering - methods</subject><subject>Humans</subject><subject>Kidney - embryology</subject><subject>Kidney - metabolism</subject><subject>Moloney murine leukemia virus</subject><subject>Moloney murine leukemia virus - genetics</subject><subject>Recombinant Proteins - biosynthesis</subject><subject>Transfection - methods</subject><issn>8756-7938</issn><issn>1520-6033</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0EtP4zAQB3ALgaA8DnwBlAsrcQh47PiRIxQoSMCiwrJHy04nuwE36dopkG9PUKtyQnuaw_zmoT8h-0CPgTI4cTPKM6BarJEBCEZTSTlfJwOthExVzvUW2Y7xmVKqqWSbZAsyKSQIGJDLMbahea2C9ck5-uoVQ5c0ZdL-xeSimHSxqTEd45-5t611HpMR1n3nfRYwxqqpk4cutjjdJRul9RH3lnWH_Lq8eBxepTc_R9fD05u0yBgTqVOFyymTjjmhHUrrrALL9AQUy6UUimNGcyolSCGUzUuwgFqCUwhc85LvkB-LvbPQ_JtjbM20igV6b2ts5tEoyDIGufovhJwBKCV6eLSARWhiDFiaWaimNnQGqPlM16zS7e3BcuncTXHyJZdx9uBwCWwsrC-DrYsqfjnBheTq8ztYuLfKY_f9RXP2eD9eHU8XM1Uf-PtqxoYXIxVXwvy-G5m7J32vn3JqbvkHHVmdtA</recordid><startdate>20031101</startdate><enddate>20031101</enddate><creator>Vaillancourt, Peter</creator><creator>Felts, Katherine A.</creator><general>American Chemical Society</general><general>American Institute of Chemical Engineers</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20031101</creationdate><title>Retroviral Delivery of the Ecdysone-Regulatable Gene Expression System</title><author>Vaillancourt, Peter ; Felts, Katherine A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4225-b7cb9026b2b58be6aba71a28d172966573e40906616557a9f1a1e861b7e1383f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Cell Line</topic><topic>CHO Cells</topic><topic>Cricetinae</topic><topic>Cricetulus</topic><topic>Ecdysone - biosynthesis</topic><topic>Ecdysone - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation - genetics</topic><topic>Gene Targeting - methods</topic><topic>Genetic Engineering - methods</topic><topic>Humans</topic><topic>Kidney - embryology</topic><topic>Kidney - metabolism</topic><topic>Moloney murine leukemia virus</topic><topic>Moloney murine leukemia virus - genetics</topic><topic>Recombinant Proteins - biosynthesis</topic><topic>Transfection - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vaillancourt, Peter</creatorcontrib><creatorcontrib>Felts, Katherine A.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biotechnology progress</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vaillancourt, Peter</au><au>Felts, Katherine A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Retroviral Delivery of the Ecdysone-Regulatable Gene Expression System</atitle><jtitle>Biotechnology progress</jtitle><addtitle>Biotechnol Progress</addtitle><date>2003-11-01</date><risdate>2003</risdate><volume>19</volume><issue>6</issue><spage>1750</spage><epage>1755</epage><pages>1750-1755</pages><issn>8756-7938</issn><eissn>1520-6033</eissn><coden>BIPRET</coden><abstract>We describe a set of Moloney Murine Leukemia Virus (MoMLV)‐based replication‐defective retroviral vectors for delivery of the ecdysone‐inducible system into mammalian cells. 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| ispartof | Biotechnology progress, 2003-11, Vol.19 (6), p.1750-1755 |
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| language | eng |
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| source | MEDLINE; Wiley Online Library All Journals |
| subjects | Animals Biological and medical sciences Biotechnology Cell Line CHO Cells Cricetinae Cricetulus Ecdysone - biosynthesis Ecdysone - genetics Fundamental and applied biological sciences. Psychology Gene Expression Regulation - genetics Gene Targeting - methods Genetic Engineering - methods Humans Kidney - embryology Kidney - metabolism Moloney murine leukemia virus Moloney murine leukemia virus - genetics Recombinant Proteins - biosynthesis Transfection - methods |
| title | Retroviral Delivery of the Ecdysone-Regulatable Gene Expression System |
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