Proteome Analysis of Antibody-Expressing CHO Cells in Response to Hyperosmotic Pressure

To better understand intracellular responses to hyperosmotic pressure of recombinant Chinese hamster ovary (rCHO) cells expressing an antibody, we have taken a proteomics approach. Using two‐dimensional electrophoresis and mass spectrometry, a proteome profile of rCHO cells comprising 23 identified...

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Veröffentlicht in:	Biotechnology progress 2003, Vol.19 (6), p.1734-1741
Hauptverfasser:	Lee, Moon Sue, Kim, Kyoung Wook, Kim, Young Hwan, Lee, Gyun Min
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Biological and medical sciences Biotechnology Cell Division - physiology CHO Cells - cytology CHO Cells - metabolism Cricetinae Cricetulus Fundamental and applied biological sciences. Psychology Gene Expression Regulation - physiology Hepatitis B Antibodies - biosynthesis Hepatitis B Antibodies - genetics Mechanotransduction, Cellular - physiology Molecular Sequence Data Osmotic Pressure Proteome - analysis Proteome - biosynthesis Proteome - genetics proteomes Proteomics - methods Recombinant Proteins - biosynthesis Sequence Analysis, Protein - methods
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