Role of CBP/p300 and SRC-1 in Transcriptional Regulation of the Pulmonary Surfactant Protein-A (SP-A) Gene by Thyroid Transcription Factor-1 (TTF-1)
Surfactant protein-A(SP-A) gene expression is developmentally regulated in fetal lung type II cells and is enhanced by cAMP. cAMP stimulation ofSP-A gene expression is mediated by protein kinase A (PKA) phosphorylation of thyroid transcription factor 1 (TTF-1), expressed selectively in developing lu...
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| Veröffentlicht in: | The Journal of biological chemistry 2002-01, Vol.277 (4), p.2997-3005 |
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| creator | Yi, Ming Tong, Guo-Xia Murry, Barbara Mendelson, Carole R. |
| description | Surfactant protein-A(SP-A) gene expression is developmentally regulated in fetal lung type II cells and is enhanced by cAMP. cAMP stimulation ofSP-A gene expression is mediated by protein kinase A (PKA) phosphorylation of thyroid transcription factor 1 (TTF-1), expressed selectively in developing lung epithelium. In this study, we analyzed roles of CREB-binding protein (CBP) and steroid receptor coactivator-1 (SRC-1) in TTF-1 regulation of SP-A expression. Upon differentiation of human fetal lung in culture, nuclear localization of CBP, SRC-1, and TTF-1 increased in ductular epithelium in association with type II cell differentiation and induction of SP-A expression. In transient transfections, CBP and SRC-1 acted synergistically with TTF-1 to increase SP-A promoter activity. Overexpression of PKA catalytic subunit enhanced hSP-A promoter activation by SRC-1 plus TTF-1. Adenoviral E1A overexpression reduced TTF-1 ± SRC-1 induction of SP-A promoter activity, suggesting a role of endogenous CBP/p300. TTF-1 interacted with SRC-1 and CBPin vitro. SRC-1 immunodepletion from type II cell nuclear extracts reduced binding to the TTF-1 binding element upstream ofSP-A gene. In cultured type II cells, cAMP increased TTF-1 acetylation. This suggests that cAMP-mediated TTF-1 phosphorylation facilitates interaction with CBP and SRC-1, resulting in its hyperacetylation, further enhancing TTF-1 DNA-binding and transcriptional activity. |
| doi_str_mv | 10.1074/jbc.M109793200 |
| format | Article |
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In this study, we analyzed roles of CREB-binding protein (CBP) and steroid receptor coactivator-1 (SRC-1) in TTF-1 regulation of SP-A expression. Upon differentiation of human fetal lung in culture, nuclear localization of CBP, SRC-1, and TTF-1 increased in ductular epithelium in association with type II cell differentiation and induction of SP-A expression. In transient transfections, CBP and SRC-1 acted synergistically with TTF-1 to increase SP-A promoter activity. Overexpression of PKA catalytic subunit enhanced hSP-A promoter activation by SRC-1 plus TTF-1. Adenoviral E1A overexpression reduced TTF-1 ± SRC-1 induction of SP-A promoter activity, suggesting a role of endogenous CBP/p300. TTF-1 interacted with SRC-1 and CBPin vitro. SRC-1 immunodepletion from type II cell nuclear extracts reduced binding to the TTF-1 binding element upstream ofSP-A gene. In cultured type II cells, cAMP increased TTF-1 acetylation. This suggests that cAMP-mediated TTF-1 phosphorylation facilitates interaction with CBP and SRC-1, resulting in its hyperacetylation, further enhancing TTF-1 DNA-binding and transcriptional activity.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1074/jbc.M109793200</identifier><identifier>PMID: 11713256</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adenoviridae - metabolism ; Catalytic Domain ; CBP/p300 protein ; Cell Differentiation ; Cell Line ; Cells, Cultured ; CREB-Binding Protein ; Cyclic AMP - metabolism ; Cyclic AMP-Dependent Protein Kinase Type II ; Cyclic AMP-Dependent Protein Kinases - metabolism ; DNA - metabolism ; Electrophoresis, Polyacrylamide Gel ; Glutathione Transferase - metabolism ; Histone Acetyltransferases ; Humans ; Immunoblotting ; Immunohistochemistry ; Lung - embryology ; Models, Biological ; Nuclear Proteins - metabolism ; Nuclear Proteins - physiology ; Nuclear Receptor Coactivator 1 ; Phosphorylation ; Plasmids - metabolism ; Promoter Regions, Genetic ; Protein Binding ; Proteolipids - genetics ; Proteolipids - metabolism ; Pulmonary Alveoli - metabolism ; Pulmonary Surfactant-Associated Protein A ; Pulmonary Surfactant-Associated Proteins ; Pulmonary Surfactants - genetics ; Pulmonary Surfactants - metabolism ; SP-A gene ; SRC-1 protein ; surfactant protein A ; Thyroid Nuclear Factor 1 ; thyroid transcription factor 1 ; Time Factors ; Trans-Activators - metabolism ; Trans-Activators - physiology ; Transcription Factors - metabolism ; Transcription Factors - physiology ; Transcription, Genetic ; Transfection</subject><ispartof>The Journal of biological chemistry, 2002-01, Vol.277 (4), p.2997-3005</ispartof><rights>2002 © 2002 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-9424535b708e40aebc96f80c406d0f267e771afb060eb3db4dfd811009c886c3</citedby><cites>FETCH-LOGICAL-c438t-9424535b708e40aebc96f80c406d0f267e771afb060eb3db4dfd811009c886c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11713256$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yi, Ming</creatorcontrib><creatorcontrib>Tong, Guo-Xia</creatorcontrib><creatorcontrib>Murry, Barbara</creatorcontrib><creatorcontrib>Mendelson, Carole R.</creatorcontrib><title>Role of CBP/p300 and SRC-1 in Transcriptional Regulation of the Pulmonary Surfactant Protein-A (SP-A) Gene by Thyroid Transcription Factor-1 (TTF-1)</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>Surfactant protein-A(SP-A) gene expression is developmentally regulated in fetal lung type II cells and is enhanced by cAMP. cAMP stimulation ofSP-A gene expression is mediated by protein kinase A (PKA) phosphorylation of thyroid transcription factor 1 (TTF-1), expressed selectively in developing lung epithelium. In this study, we analyzed roles of CREB-binding protein (CBP) and steroid receptor coactivator-1 (SRC-1) in TTF-1 regulation of SP-A expression. Upon differentiation of human fetal lung in culture, nuclear localization of CBP, SRC-1, and TTF-1 increased in ductular epithelium in association with type II cell differentiation and induction of SP-A expression. In transient transfections, CBP and SRC-1 acted synergistically with TTF-1 to increase SP-A promoter activity. Overexpression of PKA catalytic subunit enhanced hSP-A promoter activation by SRC-1 plus TTF-1. Adenoviral E1A overexpression reduced TTF-1 ± SRC-1 induction of SP-A promoter activity, suggesting a role of endogenous CBP/p300. TTF-1 interacted with SRC-1 and CBPin vitro. SRC-1 immunodepletion from type II cell nuclear extracts reduced binding to the TTF-1 binding element upstream ofSP-A gene. In cultured type II cells, cAMP increased TTF-1 acetylation. This suggests that cAMP-mediated TTF-1 phosphorylation facilitates interaction with CBP and SRC-1, resulting in its hyperacetylation, further enhancing TTF-1 DNA-binding and transcriptional activity.</description><subject>Adenoviridae - metabolism</subject><subject>Catalytic Domain</subject><subject>CBP/p300 protein</subject><subject>Cell Differentiation</subject><subject>Cell Line</subject><subject>Cells, Cultured</subject><subject>CREB-Binding Protein</subject><subject>Cyclic AMP - metabolism</subject><subject>Cyclic AMP-Dependent Protein Kinase Type II</subject><subject>Cyclic AMP-Dependent Protein Kinases - metabolism</subject><subject>DNA - metabolism</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Glutathione Transferase - metabolism</subject><subject>Histone Acetyltransferases</subject><subject>Humans</subject><subject>Immunoblotting</subject><subject>Immunohistochemistry</subject><subject>Lung - embryology</subject><subject>Models, Biological</subject><subject>Nuclear Proteins - metabolism</subject><subject>Nuclear Proteins - physiology</subject><subject>Nuclear Receptor Coactivator 1</subject><subject>Phosphorylation</subject><subject>Plasmids - metabolism</subject><subject>Promoter Regions, Genetic</subject><subject>Protein Binding</subject><subject>Proteolipids - genetics</subject><subject>Proteolipids - metabolism</subject><subject>Pulmonary Alveoli - metabolism</subject><subject>Pulmonary Surfactant-Associated Protein A</subject><subject>Pulmonary Surfactant-Associated Proteins</subject><subject>Pulmonary Surfactants - genetics</subject><subject>Pulmonary Surfactants - metabolism</subject><subject>SP-A gene</subject><subject>SRC-1 protein</subject><subject>surfactant protein A</subject><subject>Thyroid Nuclear Factor 1</subject><subject>thyroid transcription factor 1</subject><subject>Time Factors</subject><subject>Trans-Activators - metabolism</subject><subject>Trans-Activators - physiology</subject><subject>Transcription Factors - metabolism</subject><subject>Transcription Factors - physiology</subject><subject>Transcription, Genetic</subject><subject>Transfection</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFv0zAUxi0EYt3gyhFZHNB2SPecOLFzLBUdSENUbQ7cLMd5WTylcbETUP-P_cG4aqWJA8IXy_Lv-97T9xHyjsGcgeC3j7WZf2NQijJLAV6QGQOZJVnOfrwkM4CUJWWaywtyGcIjxMNL9ppcMCZYlubFjDxtXI_UtXT5aX27zwCoHhq63SwTRu1AK6-HYLzdj9YNuqcbfJh6fXwcNWOHdD31u_jlD3Q7-VabUQ8jXXs3oh2SBb3erpPFDb3DAWl9oFV38M42f_vSVZQ5HydeV9UqYTdvyKtW9wHfnu8rUq0-V8svyf33u6_LxX1ieCbHpOQpz7O8FiCRg8balEUrwXAoGmjTQqAQTLc1FIB11tS8aRvJGEBppCxMdkU-nmz33v2cMIxqZ4PBvtcDuikowTiHHIr_gkymWVrkMoLzE2i8C8Fjq_be7mI4ioE69qViX-q5ryh4f3ae6h02z_i5oAh8OAGdfeh-W4-qts50uFOpEIqrtCxFhOQJwhjWL4teBWNxMNhEgRlV4-y_FvgDe3msgg</recordid><startdate>20020125</startdate><enddate>20020125</enddate><creator>Yi, Ming</creator><creator>Tong, Guo-Xia</creator><creator>Murry, Barbara</creator><creator>Mendelson, Carole R.</creator><general>Elsevier Inc</general><general>American Society for Biochemistry and Molecular Biology</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>20020125</creationdate><title>Role of CBP/p300 and SRC-1 in Transcriptional Regulation of the Pulmonary Surfactant Protein-A (SP-A) Gene by Thyroid Transcription Factor-1 (TTF-1)</title><author>Yi, Ming ; Tong, Guo-Xia ; Murry, Barbara ; Mendelson, Carole R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-9424535b708e40aebc96f80c406d0f267e771afb060eb3db4dfd811009c886c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Adenoviridae - metabolism</topic><topic>Catalytic Domain</topic><topic>CBP/p300 protein</topic><topic>Cell Differentiation</topic><topic>Cell Line</topic><topic>Cells, Cultured</topic><topic>CREB-Binding Protein</topic><topic>Cyclic AMP - metabolism</topic><topic>Cyclic AMP-Dependent Protein Kinase Type II</topic><topic>Cyclic AMP-Dependent Protein Kinases - metabolism</topic><topic>DNA - metabolism</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Glutathione Transferase - metabolism</topic><topic>Histone Acetyltransferases</topic><topic>Humans</topic><topic>Immunoblotting</topic><topic>Immunohistochemistry</topic><topic>Lung - embryology</topic><topic>Models, Biological</topic><topic>Nuclear Proteins - metabolism</topic><topic>Nuclear Proteins - physiology</topic><topic>Nuclear Receptor Coactivator 1</topic><topic>Phosphorylation</topic><topic>Plasmids - metabolism</topic><topic>Promoter Regions, Genetic</topic><topic>Protein Binding</topic><topic>Proteolipids - genetics</topic><topic>Proteolipids - metabolism</topic><topic>Pulmonary Alveoli - metabolism</topic><topic>Pulmonary Surfactant-Associated Protein A</topic><topic>Pulmonary Surfactant-Associated Proteins</topic><topic>Pulmonary Surfactants - genetics</topic><topic>Pulmonary Surfactants - metabolism</topic><topic>SP-A gene</topic><topic>SRC-1 protein</topic><topic>surfactant protein A</topic><topic>Thyroid Nuclear Factor 1</topic><topic>thyroid transcription factor 1</topic><topic>Time Factors</topic><topic>Trans-Activators - metabolism</topic><topic>Trans-Activators - physiology</topic><topic>Transcription Factors - metabolism</topic><topic>Transcription Factors - physiology</topic><topic>Transcription, Genetic</topic><topic>Transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yi, Ming</creatorcontrib><creatorcontrib>Tong, Guo-Xia</creatorcontrib><creatorcontrib>Murry, Barbara</creatorcontrib><creatorcontrib>Mendelson, Carole R.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yi, Ming</au><au>Tong, Guo-Xia</au><au>Murry, Barbara</au><au>Mendelson, Carole R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Role of CBP/p300 and SRC-1 in Transcriptional Regulation of the Pulmonary Surfactant Protein-A (SP-A) Gene by Thyroid Transcription Factor-1 (TTF-1)</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>2002-01-25</date><risdate>2002</risdate><volume>277</volume><issue>4</issue><spage>2997</spage><epage>3005</epage><pages>2997-3005</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><abstract>Surfactant protein-A(SP-A) gene expression is developmentally regulated in fetal lung type II cells and is enhanced by cAMP. cAMP stimulation ofSP-A gene expression is mediated by protein kinase A (PKA) phosphorylation of thyroid transcription factor 1 (TTF-1), expressed selectively in developing lung epithelium. In this study, we analyzed roles of CREB-binding protein (CBP) and steroid receptor coactivator-1 (SRC-1) in TTF-1 regulation of SP-A expression. Upon differentiation of human fetal lung in culture, nuclear localization of CBP, SRC-1, and TTF-1 increased in ductular epithelium in association with type II cell differentiation and induction of SP-A expression. In transient transfections, CBP and SRC-1 acted synergistically with TTF-1 to increase SP-A promoter activity. Overexpression of PKA catalytic subunit enhanced hSP-A promoter activation by SRC-1 plus TTF-1. Adenoviral E1A overexpression reduced TTF-1 ± SRC-1 induction of SP-A promoter activity, suggesting a role of endogenous CBP/p300. TTF-1 interacted with SRC-1 and CBPin vitro. SRC-1 immunodepletion from type II cell nuclear extracts reduced binding to the TTF-1 binding element upstream ofSP-A gene. In cultured type II cells, cAMP increased TTF-1 acetylation. This suggests that cAMP-mediated TTF-1 phosphorylation facilitates interaction with CBP and SRC-1, resulting in its hyperacetylation, further enhancing TTF-1 DNA-binding and transcriptional activity.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>11713256</pmid><doi>10.1074/jbc.M109793200</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adenoviridae - metabolism Catalytic Domain CBP/p300 protein Cell Differentiation Cell Line Cells, Cultured CREB-Binding Protein Cyclic AMP - metabolism Cyclic AMP-Dependent Protein Kinase Type II Cyclic AMP-Dependent Protein Kinases - metabolism DNA - metabolism Electrophoresis, Polyacrylamide Gel Glutathione Transferase - metabolism Histone Acetyltransferases Humans Immunoblotting Immunohistochemistry Lung - embryology Models, Biological Nuclear Proteins - metabolism Nuclear Proteins - physiology Nuclear Receptor Coactivator 1 Phosphorylation Plasmids - metabolism Promoter Regions, Genetic Protein Binding Proteolipids - genetics Proteolipids - metabolism Pulmonary Alveoli - metabolism Pulmonary Surfactant-Associated Protein A Pulmonary Surfactant-Associated Proteins Pulmonary Surfactants - genetics Pulmonary Surfactants - metabolism SP-A gene SRC-1 protein surfactant protein A Thyroid Nuclear Factor 1 thyroid transcription factor 1 Time Factors Trans-Activators - metabolism Trans-Activators - physiology Transcription Factors - metabolism Transcription Factors - physiology Transcription, Genetic Transfection |
| title | Role of CBP/p300 and SRC-1 in Transcriptional Regulation of the Pulmonary Surfactant Protein-A (SP-A) Gene by Thyroid Transcription Factor-1 (TTF-1) |
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