Quantification of the anticancer agent STI-571 in erythrocytes and plasma by measurement of sediment technology and liquid chromatography–tandem mass spectrometry
An isocratic high-performance liquid chromatographic method coupled to tandem mass spectrometry for the quantification of the revolutionary and promising anticancer agent STI-571 (tradenames Gleevec, Glivec, Imatinib) in blood plasma and red blood cells (RBCs) is described. The method involves measu...
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| Veröffentlicht in: | Journal of Chromatography A 2003-12, Vol.1020 (1), p.27-34 |
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| container_title | Journal of Chromatography A |
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| creator | Guetens, Gunther De Boeck, Gert Highley, Martin Dumez, Herlinde Van Oosterom, Allan T. de Bruijn, Ernst A. |
| description | An isocratic high-performance liquid chromatographic method coupled to tandem mass spectrometry for the quantification of the revolutionary and promising anticancer agent STI-571 (tradenames Gleevec, Glivec, Imatinib) in blood plasma and red blood cells (RBCs) is described. The method involves measurement of sediment technology for RBCs and a subsequent single protein precipitation step by the addition of acetonitrile to both the RBC isolate and plasma. The sample mixture was centrifuged (10 min, 3600
g), and the supernatant filtered through a HPLC filter (0.45 μm). The analytes of interest, STI-571 and the internal standard [
2H
8]STI-571 were eluted on a Waters Symmetry C
18 column (50×2.1 mm I.D., 3.5 μm particle size) using a methanol–0.05% ammonium acetate (72:28, v/v) mixture. STI-571 and [
2H
8]STI-571 were detected by electrospray tandem mass spectrometry in the positive mode, and monitored in the multiple reaction monitoring transitions 494>394 and 502 |
| doi_str_mv | 10.1016/S0021-9673(03)00775-1 |
| format | Article |
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g), and the supernatant filtered through a HPLC filter (0.45 μm). The analytes of interest, STI-571 and the internal standard [
2H
8]STI-571 were eluted on a Waters Symmetry C
18 column (50×2.1 mm I.D., 3.5 μm particle size) using a methanol–0.05% ammonium acetate (72:28, v/v) mixture. STI-571 and [
2H
8]STI-571 were detected by electrospray tandem mass spectrometry in the positive mode, and monitored in the multiple reaction monitoring transitions 494>394 and 502<394, respectively. The lower limit of quantitation of STI-571 was 2.1 ng/ml in RBCs and 1.8 ng/ml in plasma. The recovery from both plasma and RBCs was between 65 and 70%. The method proved to be robust, allowing simultaneous quantification of STI-571 in RBCs and plasma with sufficient precision, accuracy and sensitivity and is useful in monitoring the fate of this signal transduction inhibitor in whole blood of cancer patients.</description><identifier>ISSN: 0021-9673</identifier><identifier>DOI: 10.1016/S0021-9673(03)00775-1</identifier><identifier>PMID: 14661754</identifier><identifier>CODEN: JOCRAM</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analysis ; Antineoplastic agents ; Antineoplastic Agents - blood ; Benzamides ; Biological and medical sciences ; Erythrocytes - chemistry ; General aspects ; General pharmacology ; Gleevec ; Glivec ; Humans ; Imatinib ; Imatinib Mesylate ; Measurement of sediment technology ; Medical sciences ; Pharmacology. Drug treatments ; Piperazines - blood ; Pyrimidines - blood ; Reproducibility of Results ; Sensitivity and Specificity ; Spectrometry, Mass, Electrospray Ionization - methods ; STI-571</subject><ispartof>Journal of Chromatography A, 2003-12, Vol.1020 (1), p.27-34</ispartof><rights>2003 Elsevier B.V.</rights><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c457t-e0bd6c215b3b0c0894f57d256885524049582b7602d9d58699f99254ee66ca403</citedby><cites>FETCH-LOGICAL-c457t-e0bd6c215b3b0c0894f57d256885524049582b7602d9d58699f99254ee66ca403</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0021-9673(03)00775-1$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>310,311,315,782,786,791,792,3554,23939,23940,25149,27933,27934,46004</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15265699$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14661754$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Guetens, Gunther</creatorcontrib><creatorcontrib>De Boeck, Gert</creatorcontrib><creatorcontrib>Highley, Martin</creatorcontrib><creatorcontrib>Dumez, Herlinde</creatorcontrib><creatorcontrib>Van Oosterom, Allan T.</creatorcontrib><creatorcontrib>de Bruijn, Ernst A.</creatorcontrib><title>Quantification of the anticancer agent STI-571 in erythrocytes and plasma by measurement of sediment technology and liquid chromatography–tandem mass spectrometry</title><title>Journal of Chromatography A</title><addtitle>J Chromatogr A</addtitle><description>An isocratic high-performance liquid chromatographic method coupled to tandem mass spectrometry for the quantification of the revolutionary and promising anticancer agent STI-571 (tradenames Gleevec, Glivec, Imatinib) in blood plasma and red blood cells (RBCs) is described. The method involves measurement of sediment technology for RBCs and a subsequent single protein precipitation step by the addition of acetonitrile to both the RBC isolate and plasma. The sample mixture was centrifuged (10 min, 3600
g), and the supernatant filtered through a HPLC filter (0.45 μm). The analytes of interest, STI-571 and the internal standard [
2H
8]STI-571 were eluted on a Waters Symmetry C
18 column (50×2.1 mm I.D., 3.5 μm particle size) using a methanol–0.05% ammonium acetate (72:28, v/v) mixture. STI-571 and [
2H
8]STI-571 were detected by electrospray tandem mass spectrometry in the positive mode, and monitored in the multiple reaction monitoring transitions 494>394 and 502<394, respectively. The lower limit of quantitation of STI-571 was 2.1 ng/ml in RBCs and 1.8 ng/ml in plasma. The recovery from both plasma and RBCs was between 65 and 70%. The method proved to be robust, allowing simultaneous quantification of STI-571 in RBCs and plasma with sufficient precision, accuracy and sensitivity and is useful in monitoring the fate of this signal transduction inhibitor in whole blood of cancer patients.</description><subject>Analysis</subject><subject>Antineoplastic agents</subject><subject>Antineoplastic Agents - blood</subject><subject>Benzamides</subject><subject>Biological and medical sciences</subject><subject>Erythrocytes - chemistry</subject><subject>General aspects</subject><subject>General pharmacology</subject><subject>Gleevec</subject><subject>Glivec</subject><subject>Humans</subject><subject>Imatinib</subject><subject>Imatinib Mesylate</subject><subject>Measurement of sediment technology</subject><subject>Medical sciences</subject><subject>Pharmacology. Drug treatments</subject><subject>Piperazines - blood</subject><subject>Pyrimidines - blood</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><subject>Spectrometry, Mass, Electrospray Ionization - methods</subject><subject>STI-571</subject><issn>0021-9673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc-O1SAUxllonHH0ETRsNLqoQlugrIyZ-GeSSYyZcU0onN6LKaUD1KQ738FX8Ml8Eum9N87ShARyzu_7ODkfQs8oeUMJ5W9vCKlpJbloXpHmNSFCsIo-QOf_ymfocUrfCaGCiPoROqMt51Sw9hz9_rroKbvBGZ1dmHAYcN4D3mpGTwYi1juYMr65vaqYoNhNGOKa9zGYNUMqoMXzqJPXuF-xB52WCH5TFKcE1h3eGcx-CmPYrQfB6O4WZ7EpLl7nsIt63q9_fv7KpQkee50STjOYXPqQ4_oEPRz0mODp6b5A3z5-uL38XF1_-XR1-f66Mi0TuQLSW25qyvqmJ4Z0sh2YsDXjXcdY3ZJWsq7uBSe1lZZ1XMpBypq1AJwb3ZLmAr08-s4x3C2QsvIuGRhHPUFYkhK0baSQvIDsCJoYUoowqDk6r-OqKFFbJOoQidp2r0g5WySKFt3z0wdL78Heq055FODFCdDJ6HGIJQOX7jlWc1bmLty7IwdlHT8cRJWMg5KXdbHsTdng_jPKX3I8rho</recordid><startdate>20031205</startdate><enddate>20031205</enddate><creator>Guetens, Gunther</creator><creator>De Boeck, Gert</creator><creator>Highley, Martin</creator><creator>Dumez, Herlinde</creator><creator>Van Oosterom, Allan T.</creator><creator>de Bruijn, Ernst A.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20031205</creationdate><title>Quantification of the anticancer agent STI-571 in erythrocytes and plasma by measurement of sediment technology and liquid chromatography–tandem mass spectrometry</title><author>Guetens, Gunther ; De Boeck, Gert ; Highley, Martin ; Dumez, Herlinde ; Van Oosterom, Allan T. ; de Bruijn, Ernst A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c457t-e0bd6c215b3b0c0894f57d256885524049582b7602d9d58699f99254ee66ca403</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Analysis</topic><topic>Antineoplastic agents</topic><topic>Antineoplastic Agents - blood</topic><topic>Benzamides</topic><topic>Biological and medical sciences</topic><topic>Erythrocytes - chemistry</topic><topic>General aspects</topic><topic>General pharmacology</topic><topic>Gleevec</topic><topic>Glivec</topic><topic>Humans</topic><topic>Imatinib</topic><topic>Imatinib Mesylate</topic><topic>Measurement of sediment technology</topic><topic>Medical sciences</topic><topic>Pharmacology. Drug treatments</topic><topic>Piperazines - blood</topic><topic>Pyrimidines - blood</topic><topic>Reproducibility of Results</topic><topic>Sensitivity and Specificity</topic><topic>Spectrometry, Mass, Electrospray Ionization - methods</topic><topic>STI-571</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guetens, Gunther</creatorcontrib><creatorcontrib>De Boeck, Gert</creatorcontrib><creatorcontrib>Highley, Martin</creatorcontrib><creatorcontrib>Dumez, Herlinde</creatorcontrib><creatorcontrib>Van Oosterom, Allan T.</creatorcontrib><creatorcontrib>de Bruijn, Ernst A.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Chromatography A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guetens, Gunther</au><au>De Boeck, Gert</au><au>Highley, Martin</au><au>Dumez, Herlinde</au><au>Van Oosterom, Allan T.</au><au>de Bruijn, Ernst A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantification of the anticancer agent STI-571 in erythrocytes and plasma by measurement of sediment technology and liquid chromatography–tandem mass spectrometry</atitle><jtitle>Journal of Chromatography A</jtitle><addtitle>J Chromatogr A</addtitle><date>2003-12-05</date><risdate>2003</risdate><volume>1020</volume><issue>1</issue><spage>27</spage><epage>34</epage><pages>27-34</pages><issn>0021-9673</issn><coden>JOCRAM</coden><abstract>An isocratic high-performance liquid chromatographic method coupled to tandem mass spectrometry for the quantification of the revolutionary and promising anticancer agent STI-571 (tradenames Gleevec, Glivec, Imatinib) in blood plasma and red blood cells (RBCs) is described. The method involves measurement of sediment technology for RBCs and a subsequent single protein precipitation step by the addition of acetonitrile to both the RBC isolate and plasma. The sample mixture was centrifuged (10 min, 3600
g), and the supernatant filtered through a HPLC filter (0.45 μm). The analytes of interest, STI-571 and the internal standard [
2H
8]STI-571 were eluted on a Waters Symmetry C
18 column (50×2.1 mm I.D., 3.5 μm particle size) using a methanol–0.05% ammonium acetate (72:28, v/v) mixture. STI-571 and [
2H
8]STI-571 were detected by electrospray tandem mass spectrometry in the positive mode, and monitored in the multiple reaction monitoring transitions 494>394 and 502<394, respectively. The lower limit of quantitation of STI-571 was 2.1 ng/ml in RBCs and 1.8 ng/ml in plasma. The recovery from both plasma and RBCs was between 65 and 70%. The method proved to be robust, allowing simultaneous quantification of STI-571 in RBCs and plasma with sufficient precision, accuracy and sensitivity and is useful in monitoring the fate of this signal transduction inhibitor in whole blood of cancer patients.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>14661754</pmid><doi>10.1016/S0021-9673(03)00775-1</doi><tpages>8</tpages></addata></record> |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Analysis Antineoplastic agents Antineoplastic Agents - blood Benzamides Biological and medical sciences Erythrocytes - chemistry General aspects General pharmacology Gleevec Glivec Humans Imatinib Imatinib Mesylate Measurement of sediment technology Medical sciences Pharmacology. Drug treatments Piperazines - blood Pyrimidines - blood Reproducibility of Results Sensitivity and Specificity Spectrometry, Mass, Electrospray Ionization - methods STI-571 |
| title | Quantification of the anticancer agent STI-571 in erythrocytes and plasma by measurement of sediment technology and liquid chromatography–tandem mass spectrometry |
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