Genotyping of axenic and non-axenic isolates of the genus Prochlorococcus and the OMF-Synechococcus' clade by size, sequence analysis or RFLP of the Internal Transcribed Spacer of the ribosomal operon
Unité des Cyanobactéries (CNRS URA 2172), Département de Biochimie et Génétique Moléculaire, Institut Pasteur, 28 Rue du Dr Roux, 75724 Paris Cedex 15, France 1 Station Biologique de Roscoff (CNRS UPR 9042), BP 74, 29682 Roscoff Cedex, France 2 Author for correspondence: Isabelle Iteman. Tel: +33 1...
Gespeichert in:
| Veröffentlicht in: | Microbiology (Society for General Microbiology) 2002-02, Vol.148 (2), p.453-465 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 465 |
|---|---|
| container_issue | 2 |
| container_start_page | 453 |
| container_title | Microbiology (Society for General Microbiology) |
| container_volume | 148 |
| creator | Laloui, Wassila Palinska, Katarzyna A Rippka, Rosmarie Partensky, Frederic Tandeau de Marsac, Nicole Herdman, Michael Iteman, Isabelle |
| description | Unité des Cyanobactéries (CNRS URA 2172), Département de Biochimie et Génétique Moléculaire, Institut Pasteur, 28 Rue du Dr Roux, 75724 Paris Cedex 15, France 1
Station Biologique de Roscoff (CNRS UPR 9042), BP 74, 29682 Roscoff Cedex, France 2
Author for correspondence: Isabelle Iteman. Tel: +33 1 4568 8416. Fax: +33 1 40613042. e-mail: iiteman{at}pasteur.fr
PCR amplicons of the Internal Transcribed Spacer (ITS) of the rrn operon of three axenic OMF (oceanic, marine and freshwater) strains of Synechococcus (WH7803, PCC 7001 and PCC 6307, respectively) differ greatly in length from that of the axenic Prochlorococcus marinus subsp. pastoris PCC 9511 T , although these four cyanobacteria cluster relatively closely in phylogenetic trees inferred from 16S rRNA gene sequences. The ITSs of three strains (PCC 9511 T , PCC 6307 and PCC 7001) were sequenced and compared with those available for strains Prochlorococcus MED4 (CCMP 1378) and MIT9313 from genome sequencing projects. In spite of large differences in length, sequence and mean DNA base composition, conserved domains important for transcriptional antitermination and folding of the rRNA transcripts were identified in all ITSs. A new group-specific primer permitted ITS amplification even with non-axenic isolates of Prochlorococcus and one OMF- Synechococcus strain. Prochlorococcus isolates of the high-light-adapted clade (HL) differed from representatives of the low-light-adapted clade (LL) by the length of their ITS. Restriction fragment length polymorphism (RFLP) of the ITS amplicons revealed three subclusters among the HL strains. Size, sequence data and RFLP of the ITS amplicons will therefore be valuable markers for the identification of different Prochlorococcus genotypes and for their discrimination from other cyanobacterial relatives with which they often co-exist in oceanic ecosystems.
Keywords: Prochlorococcus marinus subsp. pastoris PCC 9511 T , cyanobacteria, group-specific ITS primers, oceanic, coastal marine and freshwater Synechococcus , secondary structure of the rrn operon Abbreviations: DV, divinyl; HL, high-light-adapted clade; LL, low-light-adapted clade; ITS, Internal Transcribed Spacer; OMF, oceanic, marine and freshwater; RFLP, restriction fragment length polymorphism
The GenBank accession numbers for the ITS amplicon sequences reported in this paper are AF387607 (PCC 9511 T ), AF387610 (PCC 6307), AF387608 (PCC 7001) and AF387609 (PCC 7941).
a Present address: Unité de Reche |
| doi_str_mv | 10.1099/00221287-148-2-453 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_71439678</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>71439678</sourcerecordid><originalsourceid>FETCH-LOGICAL-h301t-2a183ab53e96d651d670b0bcc6a79fb9763092cafcee3a8baa1add524b3b34d93</originalsourceid><addsrcrecordid>eNqFks1u1DAQxyMEoqXwAhyQL1AOGPyRD-eIqm6ptKgVLedo7Ew2Romd2llBeEIeC6-aFUcutmf-v5nReCbLXnP2kbO6_sSYEFyoivJcUUHzQj7JTnleFlQwxZ6mtywYZaoSJ9mLGH8wlkTGn2cnnCspClafZn-u0Pl5mazbEd8R-IXOGgKuJc47upo2-gFmjAdi7pHs0O0juQ3e9INPpzcm2Yegg3rzdUPvFoemX5VzYgZokeiFRPsbP5CID3t0BlMIDEu0KXEg3zbb22OBazdjSBq5D-CiCVZjS-4mMBiOSPL56MfE-AmDdy-zZx0MEV-t91n2fXN5f_GFbm-uri8-b2kvGZ-pgNQ66EJiXbZlwduyYpppY0qo6k7XVSlZLQx0BlGC0gAc2rYQuZZa5m0tz7J3j3mn4FMXcW5GGw0OAzj0-9hUPJd1Wan_glyJUimRJ_DNCu71iG0zBTtCWJrjkBLwdgUgGhi69CfGxn9cLgrJ-YF7_8j1dtf_tAGbNKjRmuC19am6SXvSiCbtifwL9SOy4A</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>18268824</pqid></control><display><type>article</type><title>Genotyping of axenic and non-axenic isolates of the genus Prochlorococcus and the OMF-Synechococcus' clade by size, sequence analysis or RFLP of the Internal Transcribed Spacer of the ribosomal operon</title><source>MEDLINE</source><source>PubMed Central</source><creator>Laloui, Wassila ; Palinska, Katarzyna A ; Rippka, Rosmarie ; Partensky, Frederic ; Tandeau de Marsac, Nicole ; Herdman, Michael ; Iteman, Isabelle</creator><creatorcontrib>Laloui, Wassila ; Palinska, Katarzyna A ; Rippka, Rosmarie ; Partensky, Frederic ; Tandeau de Marsac, Nicole ; Herdman, Michael ; Iteman, Isabelle</creatorcontrib><description>Unité des Cyanobactéries (CNRS URA 2172), Département de Biochimie et Génétique Moléculaire, Institut Pasteur, 28 Rue du Dr Roux, 75724 Paris Cedex 15, France 1
Station Biologique de Roscoff (CNRS UPR 9042), BP 74, 29682 Roscoff Cedex, France 2
Author for correspondence: Isabelle Iteman. Tel: +33 1 4568 8416. Fax: +33 1 40613042. e-mail: iiteman{at}pasteur.fr
PCR amplicons of the Internal Transcribed Spacer (ITS) of the rrn operon of three axenic OMF (oceanic, marine and freshwater) strains of Synechococcus (WH7803, PCC 7001 and PCC 6307, respectively) differ greatly in length from that of the axenic Prochlorococcus marinus subsp. pastoris PCC 9511 T , although these four cyanobacteria cluster relatively closely in phylogenetic trees inferred from 16S rRNA gene sequences. The ITSs of three strains (PCC 9511 T , PCC 6307 and PCC 7001) were sequenced and compared with those available for strains Prochlorococcus MED4 (CCMP 1378) and MIT9313 from genome sequencing projects. In spite of large differences in length, sequence and mean DNA base composition, conserved domains important for transcriptional antitermination and folding of the rRNA transcripts were identified in all ITSs. A new group-specific primer permitted ITS amplification even with non-axenic isolates of Prochlorococcus and one OMF- Synechococcus strain. Prochlorococcus isolates of the high-light-adapted clade (HL) differed from representatives of the low-light-adapted clade (LL) by the length of their ITS. Restriction fragment length polymorphism (RFLP) of the ITS amplicons revealed three subclusters among the HL strains. Size, sequence data and RFLP of the ITS amplicons will therefore be valuable markers for the identification of different Prochlorococcus genotypes and for their discrimination from other cyanobacterial relatives with which they often co-exist in oceanic ecosystems.
Keywords: Prochlorococcus marinus subsp. pastoris PCC 9511 T , cyanobacteria, group-specific ITS primers, oceanic, coastal marine and freshwater Synechococcus , secondary structure of the rrn operon Abbreviations: DV, divinyl; HL, high-light-adapted clade; LL, low-light-adapted clade; ITS, Internal Transcribed Spacer; OMF, oceanic, marine and freshwater; RFLP, restriction fragment length polymorphism
The GenBank accession numbers for the ITS amplicon sequences reported in this paper are AF387607 (PCC 9511 T ), AF387610 (PCC 6307), AF387608 (PCC 7001) and AF387609 (PCC 7941).
a Present address: Unité de Recherche en Génomique Végétale (INRA/CNRS), Genoscope, Centre National de Séquençage, 2, Rue Gaston Crémieux, 91057 Evry Cedex, France.
b Present address: Carl von Ossietzky University, Geomicrobiology, PO Box 2503, 26111 Oldenburg, Germany.</description><identifier>ISSN: 1350-0872</identifier><identifier>EISSN: 1465-2080</identifier><identifier>DOI: 10.1099/00221287-148-2-453</identifier><identifier>PMID: 11832509</identifier><language>eng</language><publisher>Reading: Soc General Microbiol</publisher><subject>Bacteriology ; Base Sequence ; Biological and medical sciences ; Cyanobacteria - genetics ; Cyanobacteria - isolation & purification ; DNA, Bacterial - genetics ; DNA, Ribosomal Spacer - genetics ; Fundamental and applied biological sciences. Psychology ; Genotype ; internal transcribed spacer ; Microbiology ; Molecular Sequence Data ; Operon ; Polymorphism, Restriction Fragment Length ; Prochlorococcus marinus ; rrn gene ; Sequence Analysis, DNA ; Sequence Homology, Nucleic Acid ; Synechococcus ; Systematics ; Water Microbiology</subject><ispartof>Microbiology (Society for General Microbiology), 2002-02, Vol.148 (2), p.453-465</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14253119$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11832509$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Laloui, Wassila</creatorcontrib><creatorcontrib>Palinska, Katarzyna A</creatorcontrib><creatorcontrib>Rippka, Rosmarie</creatorcontrib><creatorcontrib>Partensky, Frederic</creatorcontrib><creatorcontrib>Tandeau de Marsac, Nicole</creatorcontrib><creatorcontrib>Herdman, Michael</creatorcontrib><creatorcontrib>Iteman, Isabelle</creatorcontrib><title>Genotyping of axenic and non-axenic isolates of the genus Prochlorococcus and the OMF-Synechococcus' clade by size, sequence analysis or RFLP of the Internal Transcribed Spacer of the ribosomal operon</title><title>Microbiology (Society for General Microbiology)</title><addtitle>Microbiology</addtitle><description>Unité des Cyanobactéries (CNRS URA 2172), Département de Biochimie et Génétique Moléculaire, Institut Pasteur, 28 Rue du Dr Roux, 75724 Paris Cedex 15, France 1
Station Biologique de Roscoff (CNRS UPR 9042), BP 74, 29682 Roscoff Cedex, France 2
Author for correspondence: Isabelle Iteman. Tel: +33 1 4568 8416. Fax: +33 1 40613042. e-mail: iiteman{at}pasteur.fr
PCR amplicons of the Internal Transcribed Spacer (ITS) of the rrn operon of three axenic OMF (oceanic, marine and freshwater) strains of Synechococcus (WH7803, PCC 7001 and PCC 6307, respectively) differ greatly in length from that of the axenic Prochlorococcus marinus subsp. pastoris PCC 9511 T , although these four cyanobacteria cluster relatively closely in phylogenetic trees inferred from 16S rRNA gene sequences. The ITSs of three strains (PCC 9511 T , PCC 6307 and PCC 7001) were sequenced and compared with those available for strains Prochlorococcus MED4 (CCMP 1378) and MIT9313 from genome sequencing projects. In spite of large differences in length, sequence and mean DNA base composition, conserved domains important for transcriptional antitermination and folding of the rRNA transcripts were identified in all ITSs. A new group-specific primer permitted ITS amplification even with non-axenic isolates of Prochlorococcus and one OMF- Synechococcus strain. Prochlorococcus isolates of the high-light-adapted clade (HL) differed from representatives of the low-light-adapted clade (LL) by the length of their ITS. Restriction fragment length polymorphism (RFLP) of the ITS amplicons revealed three subclusters among the HL strains. Size, sequence data and RFLP of the ITS amplicons will therefore be valuable markers for the identification of different Prochlorococcus genotypes and for their discrimination from other cyanobacterial relatives with which they often co-exist in oceanic ecosystems.
Keywords: Prochlorococcus marinus subsp. pastoris PCC 9511 T , cyanobacteria, group-specific ITS primers, oceanic, coastal marine and freshwater Synechococcus , secondary structure of the rrn operon Abbreviations: DV, divinyl; HL, high-light-adapted clade; LL, low-light-adapted clade; ITS, Internal Transcribed Spacer; OMF, oceanic, marine and freshwater; RFLP, restriction fragment length polymorphism
The GenBank accession numbers for the ITS amplicon sequences reported in this paper are AF387607 (PCC 9511 T ), AF387610 (PCC 6307), AF387608 (PCC 7001) and AF387609 (PCC 7941).
a Present address: Unité de Recherche en Génomique Végétale (INRA/CNRS), Genoscope, Centre National de Séquençage, 2, Rue Gaston Crémieux, 91057 Evry Cedex, France.
b Present address: Carl von Ossietzky University, Geomicrobiology, PO Box 2503, 26111 Oldenburg, Germany.</description><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cyanobacteria - genetics</subject><subject>Cyanobacteria - isolation & purification</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA, Ribosomal Spacer - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genotype</subject><subject>internal transcribed spacer</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Operon</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>Prochlorococcus marinus</subject><subject>rrn gene</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>Synechococcus</subject><subject>Systematics</subject><subject>Water Microbiology</subject><issn>1350-0872</issn><issn>1465-2080</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFks1u1DAQxyMEoqXwAhyQL1AOGPyRD-eIqm6ptKgVLedo7Ew2Romd2llBeEIeC6-aFUcutmf-v5nReCbLXnP2kbO6_sSYEFyoivJcUUHzQj7JTnleFlQwxZ6mtywYZaoSJ9mLGH8wlkTGn2cnnCspClafZn-u0Pl5mazbEd8R-IXOGgKuJc47upo2-gFmjAdi7pHs0O0juQ3e9INPpzcm2Yegg3rzdUPvFoemX5VzYgZokeiFRPsbP5CID3t0BlMIDEu0KXEg3zbb22OBazdjSBq5D-CiCVZjS-4mMBiOSPL56MfE-AmDdy-zZx0MEV-t91n2fXN5f_GFbm-uri8-b2kvGZ-pgNQ66EJiXbZlwduyYpppY0qo6k7XVSlZLQx0BlGC0gAc2rYQuZZa5m0tz7J3j3mn4FMXcW5GGw0OAzj0-9hUPJd1Wan_glyJUimRJ_DNCu71iG0zBTtCWJrjkBLwdgUgGhi69CfGxn9cLgrJ-YF7_8j1dtf_tAGbNKjRmuC19am6SXvSiCbtifwL9SOy4A</recordid><startdate>20020201</startdate><enddate>20020201</enddate><creator>Laloui, Wassila</creator><creator>Palinska, Katarzyna A</creator><creator>Rippka, Rosmarie</creator><creator>Partensky, Frederic</creator><creator>Tandeau de Marsac, Nicole</creator><creator>Herdman, Michael</creator><creator>Iteman, Isabelle</creator><general>Soc General Microbiol</general><general>Society for General Microbiology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20020201</creationdate><title>Genotyping of axenic and non-axenic isolates of the genus Prochlorococcus and the OMF-Synechococcus' clade by size, sequence analysis or RFLP of the Internal Transcribed Spacer of the ribosomal operon</title><author>Laloui, Wassila ; Palinska, Katarzyna A ; Rippka, Rosmarie ; Partensky, Frederic ; Tandeau de Marsac, Nicole ; Herdman, Michael ; Iteman, Isabelle</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h301t-2a183ab53e96d651d670b0bcc6a79fb9763092cafcee3a8baa1add524b3b34d93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Bacteriology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cyanobacteria - genetics</topic><topic>Cyanobacteria - isolation & purification</topic><topic>DNA, Bacterial - genetics</topic><topic>DNA, Ribosomal Spacer - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genotype</topic><topic>internal transcribed spacer</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Operon</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>Prochlorococcus marinus</topic><topic>rrn gene</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>Synechococcus</topic><topic>Systematics</topic><topic>Water Microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Laloui, Wassila</creatorcontrib><creatorcontrib>Palinska, Katarzyna A</creatorcontrib><creatorcontrib>Rippka, Rosmarie</creatorcontrib><creatorcontrib>Partensky, Frederic</creatorcontrib><creatorcontrib>Tandeau de Marsac, Nicole</creatorcontrib><creatorcontrib>Herdman, Michael</creatorcontrib><creatorcontrib>Iteman, Isabelle</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Microbiology (Society for General Microbiology)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Laloui, Wassila</au><au>Palinska, Katarzyna A</au><au>Rippka, Rosmarie</au><au>Partensky, Frederic</au><au>Tandeau de Marsac, Nicole</au><au>Herdman, Michael</au><au>Iteman, Isabelle</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genotyping of axenic and non-axenic isolates of the genus Prochlorococcus and the OMF-Synechococcus' clade by size, sequence analysis or RFLP of the Internal Transcribed Spacer of the ribosomal operon</atitle><jtitle>Microbiology (Society for General Microbiology)</jtitle><addtitle>Microbiology</addtitle><date>2002-02-01</date><risdate>2002</risdate><volume>148</volume><issue>2</issue><spage>453</spage><epage>465</epage><pages>453-465</pages><issn>1350-0872</issn><eissn>1465-2080</eissn><abstract>Unité des Cyanobactéries (CNRS URA 2172), Département de Biochimie et Génétique Moléculaire, Institut Pasteur, 28 Rue du Dr Roux, 75724 Paris Cedex 15, France 1
Station Biologique de Roscoff (CNRS UPR 9042), BP 74, 29682 Roscoff Cedex, France 2
Author for correspondence: Isabelle Iteman. Tel: +33 1 4568 8416. Fax: +33 1 40613042. e-mail: iiteman{at}pasteur.fr
PCR amplicons of the Internal Transcribed Spacer (ITS) of the rrn operon of three axenic OMF (oceanic, marine and freshwater) strains of Synechococcus (WH7803, PCC 7001 and PCC 6307, respectively) differ greatly in length from that of the axenic Prochlorococcus marinus subsp. pastoris PCC 9511 T , although these four cyanobacteria cluster relatively closely in phylogenetic trees inferred from 16S rRNA gene sequences. The ITSs of three strains (PCC 9511 T , PCC 6307 and PCC 7001) were sequenced and compared with those available for strains Prochlorococcus MED4 (CCMP 1378) and MIT9313 from genome sequencing projects. In spite of large differences in length, sequence and mean DNA base composition, conserved domains important for transcriptional antitermination and folding of the rRNA transcripts were identified in all ITSs. A new group-specific primer permitted ITS amplification even with non-axenic isolates of Prochlorococcus and one OMF- Synechococcus strain. Prochlorococcus isolates of the high-light-adapted clade (HL) differed from representatives of the low-light-adapted clade (LL) by the length of their ITS. Restriction fragment length polymorphism (RFLP) of the ITS amplicons revealed three subclusters among the HL strains. Size, sequence data and RFLP of the ITS amplicons will therefore be valuable markers for the identification of different Prochlorococcus genotypes and for their discrimination from other cyanobacterial relatives with which they often co-exist in oceanic ecosystems.
Keywords: Prochlorococcus marinus subsp. pastoris PCC 9511 T , cyanobacteria, group-specific ITS primers, oceanic, coastal marine and freshwater Synechococcus , secondary structure of the rrn operon Abbreviations: DV, divinyl; HL, high-light-adapted clade; LL, low-light-adapted clade; ITS, Internal Transcribed Spacer; OMF, oceanic, marine and freshwater; RFLP, restriction fragment length polymorphism
The GenBank accession numbers for the ITS amplicon sequences reported in this paper are AF387607 (PCC 9511 T ), AF387610 (PCC 6307), AF387608 (PCC 7001) and AF387609 (PCC 7941).
a Present address: Unité de Recherche en Génomique Végétale (INRA/CNRS), Genoscope, Centre National de Séquençage, 2, Rue Gaston Crémieux, 91057 Evry Cedex, France.
b Present address: Carl von Ossietzky University, Geomicrobiology, PO Box 2503, 26111 Oldenburg, Germany.</abstract><cop>Reading</cop><pub>Soc General Microbiol</pub><pmid>11832509</pmid><doi>10.1099/00221287-148-2-453</doi><tpages>13</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1350-0872 |
| ispartof | Microbiology (Society for General Microbiology), 2002-02, Vol.148 (2), p.453-465 |
| issn | 1350-0872 1465-2080 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_71439678 |
| source | MEDLINE; PubMed Central |
| subjects | Bacteriology Base Sequence Biological and medical sciences Cyanobacteria - genetics Cyanobacteria - isolation & purification DNA, Bacterial - genetics DNA, Ribosomal Spacer - genetics Fundamental and applied biological sciences. Psychology Genotype internal transcribed spacer Microbiology Molecular Sequence Data Operon Polymorphism, Restriction Fragment Length Prochlorococcus marinus rrn gene Sequence Analysis, DNA Sequence Homology, Nucleic Acid Synechococcus Systematics Water Microbiology |
| title | Genotyping of axenic and non-axenic isolates of the genus Prochlorococcus and the OMF-Synechococcus' clade by size, sequence analysis or RFLP of the Internal Transcribed Spacer of the ribosomal operon |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-03T16%3A49%3A57IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Genotyping%20of%20axenic%20and%20non-axenic%20isolates%20of%20the%20genus%20Prochlorococcus%20and%20the%20OMF-Synechococcus'%20clade%20by%20size,%20sequence%20analysis%20or%20RFLP%20of%20the%20Internal%20Transcribed%20Spacer%20of%20the%20ribosomal%20operon&rft.jtitle=Microbiology%20(Society%20for%20General%20Microbiology)&rft.au=Laloui,%20Wassila&rft.date=2002-02-01&rft.volume=148&rft.issue=2&rft.spage=453&rft.epage=465&rft.pages=453-465&rft.issn=1350-0872&rft.eissn=1465-2080&rft_id=info:doi/10.1099/00221287-148-2-453&rft_dat=%3Cproquest_pubme%3E71439678%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=18268824&rft_id=info:pmid/11832509&rfr_iscdi=true |