Switching off HSP70 and i-NOS to study their role in normal and H2O2-stressed human fibroblasts

i-NOS and HSP70 antisense oligonucleotides were used to study the role of the two well known stress-regulated molecules on cell survival of both untreated control, and H2O2-stressed human fibroblasts. Cell survival was assessed either by LDH release or by MTT assay. The levels of cytosolic i-NOS and...

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Veröffentlicht in:	Life sciences (1973) 2003-12, Vol.74 (6), p.757-769
Hauptverfasser:	Renis, Marcella, Cardile, Venera, Grasso, Salvatore, Palumbo, Maddalena, Scifo, Christian
Format:	Artikel
Sprache:	eng
Schlagworte:	Cell Line, Transformed Cell Survival - drug effects Fibroblasts - drug effects Fibroblasts - metabolism Fibroblasts - pathology Formazans - metabolism HSP70 Heat-Shock Proteins - genetics HSP70 Heat-Shock Proteins - metabolism Humans Hydrogen Peroxide - metabolism Hydrogen Peroxide - pharmacology L-Lactate Dehydrogenase - metabolism Nitric Oxide Synthase - genetics Nitric Oxide Synthase - metabolism Nitric Oxide Synthase Type II Oligonucleotides, Antisense - pharmacology Oxidative Stress Reactive Oxygen Species - metabolism Tetrazolium Salts - metabolism Transfection
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