An optimized method for Aspergillus niger spore production on natural carrier substrates
Aspergillus niger spores have wide ranging applications in the fermentation industry as well as in wastewater treatment. We present an optimized method for production of A. niger spores on natural substrates such as rice, split pea, and millet. The specific productivity (number of spores per gram of...
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| Veröffentlicht in: | Biotechnology progress 2003-11, Vol.19 (6), p.1683-1688 |
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| container_title | Biotechnology progress |
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| creator | BAPAT, Prashant M KUNDU, Sucharita WANGIKAR, Pramod P |
| description | Aspergillus niger spores have wide ranging applications in the fermentation industry as well as in wastewater treatment. We present an optimized method for production of A. niger spores on natural substrates such as rice, split pea, and millet. The specific productivity (number of spores per gram of dry substrate) was 31-fold greater and volumetric productivity was 750-fold greater compared to agar slopes. The important process variables were incubation temperature, moisture content, and inoculum quantity. We find that the optimal condition for total spore count is different from the viable spore count for millet. The optimum lies in a narrow region defined by the process parameters. Of the three substrates tested split pea gave the highest specific spore productivity of 3.1 x 10(10) spores per gram of dry substrate. This is the first report of systematic study on the effect of process parameters on spore viability. The method of A. niger spore production on natural substrate appears advantageous as compared to the currently practiced method in terms of scale-up, cost, and ease of operation. |
| doi_str_mv | 10.1021/bp0341141 |
| format | Article |
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We present an optimized method for production of A. niger spores on natural substrates such as rice, split pea, and millet. The specific productivity (number of spores per gram of dry substrate) was 31-fold greater and volumetric productivity was 750-fold greater compared to agar slopes. The important process variables were incubation temperature, moisture content, and inoculum quantity. We find that the optimal condition for total spore count is different from the viable spore count for millet. The optimum lies in a narrow region defined by the process parameters. Of the three substrates tested split pea gave the highest specific spore productivity of 3.1 x 10(10) spores per gram of dry substrate. This is the first report of systematic study on the effect of process parameters on spore viability. 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We present an optimized method for production of A. niger spores on natural substrates such as rice, split pea, and millet. The specific productivity (number of spores per gram of dry substrate) was 31-fold greater and volumetric productivity was 750-fold greater compared to agar slopes. The important process variables were incubation temperature, moisture content, and inoculum quantity. We find that the optimal condition for total spore count is different from the viable spore count for millet. The optimum lies in a narrow region defined by the process parameters. Of the three substrates tested split pea gave the highest specific spore productivity of 3.1 x 10(10) spores per gram of dry substrate. This is the first report of systematic study on the effect of process parameters on spore viability. The method of A. niger spore production on natural substrate appears advantageous as compared to the currently practiced method in terms of scale-up, cost, and ease of operation.</description><subject>Aspergillus niger</subject><subject>Aspergillus niger - cytology</subject><subject>Aspergillus niger - growth & development</subject><subject>Biological and medical sciences</subject><subject>Bioreactors - microbiology</subject><subject>Biotechnology</subject><subject>Cell Culture Techniques - methods</subject><subject>Cell Division - physiology</subject><subject>Cell Survival - physiology</subject><subject>Computer Simulation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Models, Biological</subject><subject>Oryza - microbiology</subject><subject>Pennisetum - microbiology</subject><subject>Pisum sativum - microbiology</subject><subject>Quality Control</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><subject>Spores - cytology</subject><subject>Spores - growth & development</subject><subject>Temperature</subject><issn>8756-7938</issn><issn>1520-6033</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0LtOwzAUBmALgWgpDLwA8gISQ8D2iS8Zq4qbVIkFJLbIcexilBu2M8DTk6oVjExn-c6vc36Ezim5oYTR22ogkFOa0wM0p5yRTBCAQzRXkotMFqBm6CTGD0KIIoIdoxnNBRc0Z3P0tuxwPyTf-m9b49am977Grg94GQcbNr5pxog7v7EBx6EPFg-hr0eTfD_tdbjTaQy6wUaH4LdmrGIKOtl4io6cbqI9288Fer2_e1k9Zuvnh6fVcp0ZkEXKqLTESKMLVnEwYIxz0mlFTA6kNlJaZSzXOa-lJBVT1kEtOa2YBlCcigIW6GqXOx32OdqYytZHY5tGd7YfYylpDtPX5F9IC0bF1OIEr3fQhD7GYF05BN_q8FVSUm77Ln_7nuzFPnSsWlv_yX3BE7jcAx2NblzQnfHxz3HgAoSCH3MsiIg</recordid><startdate>20031101</startdate><enddate>20031101</enddate><creator>BAPAT, Prashant M</creator><creator>KUNDU, Sucharita</creator><creator>WANGIKAR, Pramod P</creator><general>American Chemical Society</general><general>American Institute of Chemical Engineers</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>7UA</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20031101</creationdate><title>An optimized method for Aspergillus niger spore production on natural carrier substrates</title><author>BAPAT, Prashant M ; KUNDU, Sucharita ; WANGIKAR, Pramod P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c379t-17e0c7ca92b53c3ccff7fa80c430dc77e8ce5a45d770b28ef3d751b2a33851693</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Aspergillus niger</topic><topic>Aspergillus niger - cytology</topic><topic>Aspergillus niger - growth & development</topic><topic>Biological and medical sciences</topic><topic>Bioreactors - microbiology</topic><topic>Biotechnology</topic><topic>Cell Culture Techniques - methods</topic><topic>Cell Division - physiology</topic><topic>Cell Survival - physiology</topic><topic>Computer Simulation</topic><topic>Fundamental and applied biological sciences. 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We present an optimized method for production of A. niger spores on natural substrates such as rice, split pea, and millet. The specific productivity (number of spores per gram of dry substrate) was 31-fold greater and volumetric productivity was 750-fold greater compared to agar slopes. The important process variables were incubation temperature, moisture content, and inoculum quantity. We find that the optimal condition for total spore count is different from the viable spore count for millet. The optimum lies in a narrow region defined by the process parameters. Of the three substrates tested split pea gave the highest specific spore productivity of 3.1 x 10(10) spores per gram of dry substrate. This is the first report of systematic study on the effect of process parameters on spore viability. 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| subjects | Aspergillus niger Aspergillus niger - cytology Aspergillus niger - growth & development Biological and medical sciences Bioreactors - microbiology Biotechnology Cell Culture Techniques - methods Cell Division - physiology Cell Survival - physiology Computer Simulation Fundamental and applied biological sciences. Psychology Models, Biological Oryza - microbiology Pennisetum - microbiology Pisum sativum - microbiology Quality Control Reproducibility of Results Sensitivity and Specificity Spores - cytology Spores - growth & development Temperature |
| title | An optimized method for Aspergillus niger spore production on natural carrier substrates |
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