Ex vivo expansion of polyclonal and antigen-specific cytotoxic T lymphocytes by artificial APCs expressing ligands for the T-cell receptor, CD28 and 4-1BB
The ex vivo priming and expansion of human cytotoxic T lymphocytes (CTLs) has potential for use in immunotherapy applications for cancer and infectious diseases. To overcome the difficulty in obtaining sufficient numbers of CTLs, we have developed artificial antigen-presenting cells (aAPCs) expressi...
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| Veröffentlicht in: | Nature biotechnology 2002-02, Vol.20 (2), p.143-148 |
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| creator | June, Carl H Maus, Marcela V Thomas, Anna K Leonard, Debra G.B Allman, David Addya, Kathakali Schlienger, Katia Riley, James L |
| description | The
ex vivo
priming and expansion of human cytotoxic T lymphocytes (CTLs) has potential for use in immunotherapy applications for cancer and infectious diseases. To overcome the difficulty in obtaining sufficient numbers of CTLs, we have developed artificial antigen-presenting cells (aAPCs) expressing ligands for the T-cell receptor (TCR) and the CD28 and 4-1BB co-stimulatory surface molecules. These aAPCs reproducibly activate and rapidly expand polyclonal or antigen-specific CD8
+
T cells. The starting repertoire of CD8
+
T cells was preserved during culture. Furthermore, apoptosis of cultured CD8
+
T cells was diminished by this approach. This approach may have important therapeutic implications for adoptive immunotherapy. |
| doi_str_mv | 10.1038/nbt0202-143 |
| format | Article |
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ex vivo
priming and expansion of human cytotoxic T lymphocytes (CTLs) has potential for use in immunotherapy applications for cancer and infectious diseases. To overcome the difficulty in obtaining sufficient numbers of CTLs, we have developed artificial antigen-presenting cells (aAPCs) expressing ligands for the T-cell receptor (TCR) and the CD28 and 4-1BB co-stimulatory surface molecules. These aAPCs reproducibly activate and rapidly expand polyclonal or antigen-specific CD8
+
T cells. The starting repertoire of CD8
+
T cells was preserved during culture. Furthermore, apoptosis of cultured CD8
+
T cells was diminished by this approach. This approach may have important therapeutic implications for adoptive immunotherapy.</description><identifier>ISSN: 1087-0156</identifier><identifier>EISSN: 1546-1696</identifier><identifier>DOI: 10.1038/nbt0202-143</identifier><identifier>PMID: 11821859</identifier><identifier>CODEN: NABIF9</identifier><language>eng</language><publisher>New York: Nature Publishing Group US</publisher><subject>4-1BB antigen ; Agriculture ; Antibodies ; Antigen-Presenting Cells - immunology ; Antigens ; Antigens, CD ; Apoptosis ; Bioinformatics ; Biological and medical sciences ; Biomedical and Life Sciences ; Biomedical Engineering/Biotechnology ; Biomedicine ; Biotechnology ; Cancer ; CD28 antigen ; CD28 Antigens - metabolism ; CD8 antigen ; CD8-Positive T-Lymphocytes - metabolism ; Cell Line ; Cell Separation ; Cells, Cultured ; Cloning ; Coculture Techniques ; Cytomegalovirus ; Cytotoxicity ; Flow Cytometry ; Fundamental and applied biological sciences. Psychology ; Health. Pharmaceutical industry ; Humans ; Immunotherapy ; Immunotherapy, Adoptive - methods ; Industrial applications and implications. Economical aspects ; Infectious diseases ; K562 Cells ; Life Sciences ; Ligands ; Lymphocytes ; Medical research ; Miscellaneous ; Protein Binding ; Receptors, Antigen, T-Cell - metabolism ; Receptors, Nerve Growth Factor - metabolism ; Receptors, Tumor Necrosis Factor - metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; T-Lymphocytes, Cytotoxic - cytology ; T-Lymphocytes, Cytotoxic - immunology ; Time Factors ; Tumor Necrosis Factor Receptor Superfamily, Member 9 ; Tumor necrosis factor-TNF</subject><ispartof>Nature biotechnology, 2002-02, Vol.20 (2), p.143-148</ispartof><rights>Springer Nature America, Inc. 2002</rights><rights>2002 INIST-CNRS</rights><rights>COPYRIGHT 2002 Nature Publishing Group</rights><rights>Copyright Nature Publishing Group Feb 2002</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c543t-afae3b1b2efae0aee434e5aa37177acdc89d3905be226c7d47cb4998926008683</citedby><cites>FETCH-LOGICAL-c543t-afae3b1b2efae0aee434e5aa37177acdc89d3905be226c7d47cb4998926008683</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,2727,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13538458$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11821859$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>June, Carl H</creatorcontrib><creatorcontrib>Maus, Marcela V</creatorcontrib><creatorcontrib>Thomas, Anna K</creatorcontrib><creatorcontrib>Leonard, Debra G.B</creatorcontrib><creatorcontrib>Allman, David</creatorcontrib><creatorcontrib>Addya, Kathakali</creatorcontrib><creatorcontrib>Schlienger, Katia</creatorcontrib><creatorcontrib>Riley, James L</creatorcontrib><title>Ex vivo expansion of polyclonal and antigen-specific cytotoxic T lymphocytes by artificial APCs expressing ligands for the T-cell receptor, CD28 and 4-1BB</title><title>Nature biotechnology</title><addtitle>Nat Biotechnol</addtitle><addtitle>Nat Biotechnol</addtitle><description>The
ex vivo
priming and expansion of human cytotoxic T lymphocytes (CTLs) has potential for use in immunotherapy applications for cancer and infectious diseases. To overcome the difficulty in obtaining sufficient numbers of CTLs, we have developed artificial antigen-presenting cells (aAPCs) expressing ligands for the T-cell receptor (TCR) and the CD28 and 4-1BB co-stimulatory surface molecules. These aAPCs reproducibly activate and rapidly expand polyclonal or antigen-specific CD8
+
T cells. The starting repertoire of CD8
+
T cells was preserved during culture. Furthermore, apoptosis of cultured CD8
+
T cells was diminished by this approach. This approach may have important therapeutic implications for adoptive immunotherapy.</description><subject>4-1BB antigen</subject><subject>Agriculture</subject><subject>Antibodies</subject><subject>Antigen-Presenting Cells - immunology</subject><subject>Antigens</subject><subject>Antigens, CD</subject><subject>Apoptosis</subject><subject>Bioinformatics</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedical Engineering/Biotechnology</subject><subject>Biomedicine</subject><subject>Biotechnology</subject><subject>Cancer</subject><subject>CD28 antigen</subject><subject>CD28 Antigens - metabolism</subject><subject>CD8 antigen</subject><subject>CD8-Positive T-Lymphocytes - metabolism</subject><subject>Cell Line</subject><subject>Cell Separation</subject><subject>Cells, Cultured</subject><subject>Cloning</subject><subject>Coculture Techniques</subject><subject>Cytomegalovirus</subject><subject>Cytotoxicity</subject><subject>Flow Cytometry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Health. Pharmaceutical industry</subject><subject>Humans</subject><subject>Immunotherapy</subject><subject>Immunotherapy, Adoptive - methods</subject><subject>Industrial applications and implications. Economical aspects</subject><subject>Infectious diseases</subject><subject>K562 Cells</subject><subject>Life Sciences</subject><subject>Ligands</subject><subject>Lymphocytes</subject><subject>Medical research</subject><subject>Miscellaneous</subject><subject>Protein Binding</subject><subject>Receptors, Antigen, T-Cell - metabolism</subject><subject>Receptors, Nerve Growth Factor - metabolism</subject><subject>Receptors, Tumor Necrosis Factor - metabolism</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>T-Lymphocytes, Cytotoxic - cytology</subject><subject>T-Lymphocytes, Cytotoxic - immunology</subject><subject>Time Factors</subject><subject>Tumor Necrosis Factor Receptor Superfamily, Member 9</subject><subject>Tumor necrosis factor-TNF</subject><issn>1087-0156</issn><issn>1546-1696</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqNkmFr1DAYx4sobp6-8r3EgYK4zqRp0vTl7Zw6GEz09G1J06ddRi-pSTruvso-7VLv8JwImhDykPye_5P8eZLkOcEnBFPxztQBZzhLSU4fJIeE5TwlvOQPY4xFkWLC-EHyxPtrjDHPOX-cHBAiMiJYeZjcnq3Rjb6xCNaDNF5bg2yLBttvVG-N7JE0TVxBd2BSP4DSrVZIbYINdh2jJeo3q-HKxhPwqN4g6cKE6Jg6_7zwk64D77XpUK-7qOZRax0KV4CWqYK-Rw4UDMG6Y7R4n4mfBfOUnJ4-TR61svfwbLfPkm8fzpaLT-nF5cfzxfwiVSynIZWtBFqTOoMYYAmQ0xyYlLQgRSFVo0TZ0BKzGrKMq6LJC1XnZSnKjGMsuKCz5PVWd3D2xwg-VCvtp5dJA3b0VRGNpUXJ_gkSQTlhjEbw6A_w2o4uuumrLI5C5HhSO9lCneyh0qa1wUkVZwMrrayBVsfzOSkxIazkOCa8uZcQmQDr0MnR--r865f_Zy-_32ffblnlrPcO2mpweiXdpiK4mjqs2nVYNRkxS17s_jbWK2j27K6lIvBqB0ivZN86aZT2e44yKnI22X685Xy8Mh24vUl_r_tyixsZRge_9H7rfnoHXbjwaA</recordid><startdate>20020201</startdate><enddate>20020201</enddate><creator>June, Carl H</creator><creator>Maus, Marcela V</creator><creator>Thomas, Anna K</creator><creator>Leonard, Debra G.B</creator><creator>Allman, David</creator><creator>Addya, Kathakali</creator><creator>Schlienger, Katia</creator><creator>Riley, James L</creator><general>Nature Publishing Group US</general><general>Nature</general><general>Nature Publishing Group</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QO</scope><scope>7QP</scope><scope>7QR</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M2P</scope><scope>M7P</scope><scope>M7S</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>Q9U</scope><scope>RC3</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20020201</creationdate><title>Ex vivo expansion of polyclonal and antigen-specific cytotoxic T lymphocytes by artificial APCs expressing ligands for the T-cell receptor, CD28 and 4-1BB</title><author>June, Carl H ; Maus, Marcela V ; Thomas, Anna K ; Leonard, Debra G.B ; Allman, David ; Addya, Kathakali ; Schlienger, Katia ; Riley, James L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c543t-afae3b1b2efae0aee434e5aa37177acdc89d3905be226c7d47cb4998926008683</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>4-1BB antigen</topic><topic>Agriculture</topic><topic>Antibodies</topic><topic>Antigen-Presenting Cells - immunology</topic><topic>Antigens</topic><topic>Antigens, CD</topic><topic>Apoptosis</topic><topic>Bioinformatics</topic><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedical Engineering/Biotechnology</topic><topic>Biomedicine</topic><topic>Biotechnology</topic><topic>Cancer</topic><topic>CD28 antigen</topic><topic>CD28 Antigens - metabolism</topic><topic>CD8 antigen</topic><topic>CD8-Positive T-Lymphocytes - metabolism</topic><topic>Cell Line</topic><topic>Cell Separation</topic><topic>Cells, Cultured</topic><topic>Cloning</topic><topic>Coculture Techniques</topic><topic>Cytomegalovirus</topic><topic>Cytotoxicity</topic><topic>Flow Cytometry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Health. Pharmaceutical industry</topic><topic>Humans</topic><topic>Immunotherapy</topic><topic>Immunotherapy, Adoptive - methods</topic><topic>Industrial applications and implications. Economical aspects</topic><topic>Infectious diseases</topic><topic>K562 Cells</topic><topic>Life Sciences</topic><topic>Ligands</topic><topic>Lymphocytes</topic><topic>Medical research</topic><topic>Miscellaneous</topic><topic>Protein Binding</topic><topic>Receptors, Antigen, T-Cell - metabolism</topic><topic>Receptors, Nerve Growth Factor - metabolism</topic><topic>Receptors, Tumor Necrosis Factor - metabolism</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>T-Lymphocytes, Cytotoxic - cytology</topic><topic>T-Lymphocytes, Cytotoxic - immunology</topic><topic>Time Factors</topic><topic>Tumor Necrosis Factor Receptor Superfamily, Member 9</topic><topic>Tumor necrosis factor-TNF</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>June, Carl H</creatorcontrib><creatorcontrib>Maus, Marcela V</creatorcontrib><creatorcontrib>Thomas, Anna K</creatorcontrib><creatorcontrib>Leonard, Debra G.B</creatorcontrib><creatorcontrib>Allman, David</creatorcontrib><creatorcontrib>Addya, Kathakali</creatorcontrib><creatorcontrib>Schlienger, Katia</creatorcontrib><creatorcontrib>Riley, James L</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Nature biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>June, Carl H</au><au>Maus, Marcela V</au><au>Thomas, Anna K</au><au>Leonard, Debra G.B</au><au>Allman, David</au><au>Addya, Kathakali</au><au>Schlienger, Katia</au><au>Riley, James L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ex vivo expansion of polyclonal and antigen-specific cytotoxic T lymphocytes by artificial APCs expressing ligands for the T-cell receptor, CD28 and 4-1BB</atitle><jtitle>Nature biotechnology</jtitle><stitle>Nat Biotechnol</stitle><addtitle>Nat Biotechnol</addtitle><date>2002-02-01</date><risdate>2002</risdate><volume>20</volume><issue>2</issue><spage>143</spage><epage>148</epage><pages>143-148</pages><issn>1087-0156</issn><eissn>1546-1696</eissn><coden>NABIF9</coden><abstract>The
ex vivo
priming and expansion of human cytotoxic T lymphocytes (CTLs) has potential for use in immunotherapy applications for cancer and infectious diseases. To overcome the difficulty in obtaining sufficient numbers of CTLs, we have developed artificial antigen-presenting cells (aAPCs) expressing ligands for the T-cell receptor (TCR) and the CD28 and 4-1BB co-stimulatory surface molecules. These aAPCs reproducibly activate and rapidly expand polyclonal or antigen-specific CD8
+
T cells. The starting repertoire of CD8
+
T cells was preserved during culture. Furthermore, apoptosis of cultured CD8
+
T cells was diminished by this approach. This approach may have important therapeutic implications for adoptive immunotherapy.</abstract><cop>New York</cop><pub>Nature Publishing Group US</pub><pmid>11821859</pmid><doi>10.1038/nbt0202-143</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Nature biotechnology, 2002-02, Vol.20 (2), p.143-148 |
| issn | 1087-0156 1546-1696 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_71433795 |
| source | MEDLINE; Nature Journals Online; Alma/SFX Local Collection |
| subjects | 4-1BB antigen Agriculture Antibodies Antigen-Presenting Cells - immunology Antigens Antigens, CD Apoptosis Bioinformatics Biological and medical sciences Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biomedicine Biotechnology Cancer CD28 antigen CD28 Antigens - metabolism CD8 antigen CD8-Positive T-Lymphocytes - metabolism Cell Line Cell Separation Cells, Cultured Cloning Coculture Techniques Cytomegalovirus Cytotoxicity Flow Cytometry Fundamental and applied biological sciences. Psychology Health. Pharmaceutical industry Humans Immunotherapy Immunotherapy, Adoptive - methods Industrial applications and implications. Economical aspects Infectious diseases K562 Cells Life Sciences Ligands Lymphocytes Medical research Miscellaneous Protein Binding Receptors, Antigen, T-Cell - metabolism Receptors, Nerve Growth Factor - metabolism Receptors, Tumor Necrosis Factor - metabolism Reverse Transcriptase Polymerase Chain Reaction T-Lymphocytes, Cytotoxic - cytology T-Lymphocytes, Cytotoxic - immunology Time Factors Tumor Necrosis Factor Receptor Superfamily, Member 9 Tumor necrosis factor-TNF |
| title | Ex vivo expansion of polyclonal and antigen-specific cytotoxic T lymphocytes by artificial APCs expressing ligands for the T-cell receptor, CD28 and 4-1BB |
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