Complete Deoxygenation from a Hemoglobin Solution by an Electrochemical Method and Heat Treatment for Virus Inactivation
Hemoglobin (Hb) has been widely studied as a raw material for various types of oxygen carriers. In the purification of Hb from red blood cells including virus inactivation and denaturation of other proteins and the long‐term storage of Hb vesicles (HbV), a deoxygenation process is one of the importa...
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| Veröffentlicht in: | Biotechnology progress 2002, Vol.18 (1), p.101-107 |
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| creator | Huang, Yubin Takeoka, Shinji Sakai, Hiromi Abe, Hideki Hirayama, Junichi Ikebuchi, Kenji Ikeda, Hisami Tsuchida, Eishun |
| description | Hemoglobin (Hb) has been widely studied as a raw material for various types of oxygen carriers. In the purification of Hb from red blood cells including virus inactivation and denaturation of other proteins and the long‐term storage of Hb vesicles (HbV), a deoxygenation process is one of the important processes because of the high stability of deoxygenated Hb to heating and metHb formation. Though an oxygenated Hb solution can be deoxygenated with an artificial lung, it is difficult to reduce the oxygen partial pressure of the Hb solution to less than 10 Torr. We developed an electrochemical system for complete deoxygenation of the Hb solution at the cathode compartment using hydrogen containing nitrogen gas at the anode compartment. Oxygen in the Hb solution was reduced to OH− at the cathode compartment within several minutes at a potential value of −1.67 V and was finally converted to water by neutralization with H+ from the anode in the whole system. The resulting completely deoxygenated Hb could tolerate heat treatment at 62 °C for 10 h with no denaturation of deoxygenated Hb. The metHb formation rate of reoxygenated Hb at 37 °C was not changed after heat treatment. Furthermore, vesicular stomatitis virus (VSV) could be inactivated at an inactivation degree of more than 5.96 log by heat treatment. |
| doi_str_mv | 10.1021/bp0101233 |
| format | Article |
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In the purification of Hb from red blood cells including virus inactivation and denaturation of other proteins and the long‐term storage of Hb vesicles (HbV), a deoxygenation process is one of the important processes because of the high stability of deoxygenated Hb to heating and metHb formation. Though an oxygenated Hb solution can be deoxygenated with an artificial lung, it is difficult to reduce the oxygen partial pressure of the Hb solution to less than 10 Torr. We developed an electrochemical system for complete deoxygenation of the Hb solution at the cathode compartment using hydrogen containing nitrogen gas at the anode compartment. Oxygen in the Hb solution was reduced to OH− at the cathode compartment within several minutes at a potential value of −1.67 V and was finally converted to water by neutralization with H+ from the anode in the whole system. The resulting completely deoxygenated Hb could tolerate heat treatment at 62 °C for 10 h with no denaturation of deoxygenated Hb. The metHb formation rate of reoxygenated Hb at 37 °C was not changed after heat treatment. Furthermore, vesicular stomatitis virus (VSV) could be inactivated at an inactivation degree of more than 5.96 log by heat treatment.</description><identifier>ISSN: 8756-7938</identifier><identifier>EISSN: 1520-6033</identifier><identifier>DOI: 10.1021/bp0101233</identifier><identifier>PMID: 11822907</identifier><identifier>CODEN: BIPRET</identifier><language>eng</language><publisher>USA: American Chemical Society</publisher><subject>Animals ; Biological and medical sciences ; Biotechnology ; Blood Substitutes - chemical synthesis ; Cattle ; Drug Contamination - prevention & control ; Drug Stability ; Electrochemistry - methods ; Fundamental and applied biological sciences. Psychology ; Hemoglobins - isolation & purification ; Hemoglobins - metabolism ; Hot Temperature ; Methemoglobin - metabolism ; Oxygen - chemistry ; Partial Pressure ; Protein Denaturation ; Solutions ; Sterilization - methods ; Virus Inactivation</subject><ispartof>Biotechnology progress, 2002, Vol.18 (1), p.101-107</ispartof><rights>Copyright © 2002 American Institute of Chemical Engineers (AIChE)</rights><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5873-81074da052e6b388ae5dccc214f3c420ccf4955efbd8ca2adaae7bd152508f33</citedby><cites>FETCH-LOGICAL-c5873-81074da052e6b388ae5dccc214f3c420ccf4955efbd8ca2adaae7bd152508f33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1021%2Fbp0101233$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1021%2Fbp0101233$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,778,782,1414,4012,27910,27911,27912,45561,45562</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13477769$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11822907$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Huang, Yubin</creatorcontrib><creatorcontrib>Takeoka, Shinji</creatorcontrib><creatorcontrib>Sakai, Hiromi</creatorcontrib><creatorcontrib>Abe, Hideki</creatorcontrib><creatorcontrib>Hirayama, Junichi</creatorcontrib><creatorcontrib>Ikebuchi, Kenji</creatorcontrib><creatorcontrib>Ikeda, Hisami</creatorcontrib><creatorcontrib>Tsuchida, Eishun</creatorcontrib><title>Complete Deoxygenation from a Hemoglobin Solution by an Electrochemical Method and Heat Treatment for Virus Inactivation</title><title>Biotechnology progress</title><addtitle>Biotechnol Progress</addtitle><description>Hemoglobin (Hb) has been widely studied as a raw material for various types of oxygen carriers. In the purification of Hb from red blood cells including virus inactivation and denaturation of other proteins and the long‐term storage of Hb vesicles (HbV), a deoxygenation process is one of the important processes because of the high stability of deoxygenated Hb to heating and metHb formation. Though an oxygenated Hb solution can be deoxygenated with an artificial lung, it is difficult to reduce the oxygen partial pressure of the Hb solution to less than 10 Torr. We developed an electrochemical system for complete deoxygenation of the Hb solution at the cathode compartment using hydrogen containing nitrogen gas at the anode compartment. Oxygen in the Hb solution was reduced to OH− at the cathode compartment within several minutes at a potential value of −1.67 V and was finally converted to water by neutralization with H+ from the anode in the whole system. The resulting completely deoxygenated Hb could tolerate heat treatment at 62 °C for 10 h with no denaturation of deoxygenated Hb. The metHb formation rate of reoxygenated Hb at 37 °C was not changed after heat treatment. Furthermore, vesicular stomatitis virus (VSV) could be inactivated at an inactivation degree of more than 5.96 log by heat treatment.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Blood Substitutes - chemical synthesis</subject><subject>Cattle</subject><subject>Drug Contamination - prevention & control</subject><subject>Drug Stability</subject><subject>Electrochemistry - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hemoglobins - isolation & purification</subject><subject>Hemoglobins - metabolism</subject><subject>Hot Temperature</subject><subject>Methemoglobin - metabolism</subject><subject>Oxygen - chemistry</subject><subject>Partial Pressure</subject><subject>Protein Denaturation</subject><subject>Solutions</subject><subject>Sterilization - methods</subject><subject>Virus Inactivation</subject><issn>8756-7938</issn><issn>1520-6033</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqN0U1vEzEQBmALgWhaOPAHkC8g9bDUH-uPPdK0JJVCidoIjpbXO9sadtfB3oXk37M0UXpC5WIf5pkZaV6E3lDygRJGz8o1oYQyzp-hCRWMZJJw_hxNtBIyUwXXR-g4pe-EEE0ke4mOKNWMFURN0GYa2nUDPeALCJvtHXS296HDdQwttngObbhrQuk7fBua4aFUbrHt8GUDro_B3UPrnW3wZ-jvQzVWqrHJ9ngVx7eFrsd1iPirj0PCV511vf_1sOEVelHbJsHr_X-CVp8uV9N5tvgyu5p-XGROaMUzTYnKK0sEA1lyrS2IyjnHaF5zlzPiXJ0XQkBdVtpZZitrQZXVeAVBdM35CXq_G7uO4ecAqTetTw6axnYQhmQUzTkptH4SskIUXEj6H5DmTBbkSUg1l1QyMcLTHXQxpBShNuvoWxu3hhLzN2BzCHi0b_dDh7KF6lHuEx3Buz2wacyljrZzPj06niulZDE6unO_fQPbf28056vlzWF5tuvxqYfNocfGH0YqroT5dj0z54vb2cVyfmOW_A-zicxY</recordid><startdate>2002</startdate><enddate>2002</enddate><creator>Huang, Yubin</creator><creator>Takeoka, Shinji</creator><creator>Sakai, Hiromi</creator><creator>Abe, Hideki</creator><creator>Hirayama, Junichi</creator><creator>Ikebuchi, Kenji</creator><creator>Ikeda, Hisami</creator><creator>Tsuchida, Eishun</creator><general>American Chemical Society</general><general>American Institute of Chemical Engineers</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7TB</scope><scope>KR7</scope><scope>7X8</scope></search><sort><creationdate>2002</creationdate><title>Complete Deoxygenation from a Hemoglobin Solution by an Electrochemical Method and Heat Treatment for Virus Inactivation</title><author>Huang, Yubin ; Takeoka, Shinji ; Sakai, Hiromi ; Abe, Hideki ; Hirayama, Junichi ; Ikebuchi, Kenji ; Ikeda, Hisami ; Tsuchida, Eishun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5873-81074da052e6b388ae5dccc214f3c420ccf4955efbd8ca2adaae7bd152508f33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Blood Substitutes - chemical synthesis</topic><topic>Cattle</topic><topic>Drug Contamination - prevention & control</topic><topic>Drug Stability</topic><topic>Electrochemistry - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hemoglobins - isolation & purification</topic><topic>Hemoglobins - metabolism</topic><topic>Hot Temperature</topic><topic>Methemoglobin - metabolism</topic><topic>Oxygen - chemistry</topic><topic>Partial Pressure</topic><topic>Protein Denaturation</topic><topic>Solutions</topic><topic>Sterilization - methods</topic><topic>Virus Inactivation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Huang, Yubin</creatorcontrib><creatorcontrib>Takeoka, Shinji</creatorcontrib><creatorcontrib>Sakai, Hiromi</creatorcontrib><creatorcontrib>Abe, Hideki</creatorcontrib><creatorcontrib>Hirayama, Junichi</creatorcontrib><creatorcontrib>Ikebuchi, Kenji</creatorcontrib><creatorcontrib>Ikeda, Hisami</creatorcontrib><creatorcontrib>Tsuchida, Eishun</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Civil Engineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biotechnology progress</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Huang, Yubin</au><au>Takeoka, Shinji</au><au>Sakai, Hiromi</au><au>Abe, Hideki</au><au>Hirayama, Junichi</au><au>Ikebuchi, Kenji</au><au>Ikeda, Hisami</au><au>Tsuchida, Eishun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Complete Deoxygenation from a Hemoglobin Solution by an Electrochemical Method and Heat Treatment for Virus Inactivation</atitle><jtitle>Biotechnology progress</jtitle><addtitle>Biotechnol Progress</addtitle><date>2002</date><risdate>2002</risdate><volume>18</volume><issue>1</issue><spage>101</spage><epage>107</epage><pages>101-107</pages><issn>8756-7938</issn><eissn>1520-6033</eissn><coden>BIPRET</coden><abstract>Hemoglobin (Hb) has been widely studied as a raw material for various types of oxygen carriers. 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The resulting completely deoxygenated Hb could tolerate heat treatment at 62 °C for 10 h with no denaturation of deoxygenated Hb. The metHb formation rate of reoxygenated Hb at 37 °C was not changed after heat treatment. Furthermore, vesicular stomatitis virus (VSV) could be inactivated at an inactivation degree of more than 5.96 log by heat treatment.</abstract><cop>USA</cop><pub>American Chemical Society</pub><pmid>11822907</pmid><doi>10.1021/bp0101233</doi><tpages>7</tpages></addata></record> |
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| ispartof | Biotechnology progress, 2002, Vol.18 (1), p.101-107 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animals Biological and medical sciences Biotechnology Blood Substitutes - chemical synthesis Cattle Drug Contamination - prevention & control Drug Stability Electrochemistry - methods Fundamental and applied biological sciences. Psychology Hemoglobins - isolation & purification Hemoglobins - metabolism Hot Temperature Methemoglobin - metabolism Oxygen - chemistry Partial Pressure Protein Denaturation Solutions Sterilization - methods Virus Inactivation |
| title | Complete Deoxygenation from a Hemoglobin Solution by an Electrochemical Method and Heat Treatment for Virus Inactivation |
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