Surface-Enhanced Resonance Raman Scattering: Single-Molecule Detection in a Langmuir—Blodgett Monolayer
Surface-enhanced resonance Raman scattering (SERRS) is used for single-molecule detection from spatially resolved 1-μm2 sections of a Langmuir–Blodgett (LB) monolayer deposited onto a Ag film. The target molecule, bis (benzimidazo) thioperylene (BZP), is dispersed in an arachidic acid monomolecular...
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| Veröffentlicht in: | Applied spectroscopy 2003-06, Vol.57 (6), p.649-654 |
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| creator | Constantino, Carlos J. L. Lemma, Tibebe Antunes, Patricia A. Goulet, Paul Aroca, Ricardo |
| description | Surface-enhanced resonance Raman scattering (SERRS) is used for single-molecule detection from spatially resolved 1-μm2 sections of a Langmuir–Blodgett (LB) monolayer deposited onto a Ag film. The target molecule, bis (benzimidazo) thioperylene (BZP), is dispersed in an arachidic acid monomolecular layer containing one BZP molecule per μm2, which is also the probing area of the Raman microscope. For concentrated samples (attomole quantities in the field of view), average SERRS, surface-enhanced fluorescence (SEF), and Raman imaging, including line mapping and global images at different temperatures, were recorded. Single-molecule SERRS spectra, obtained using an LB monolayer, present changes in bandwidth and relative intensities, highlighting the properties of single-molecule SERRS that are lost in average SERRS measurements of mixed LB monolayers obtained at the same temperatures. Also, the dilute system phenomenon of blinking is discussed with regard to results obtained from LB monolayers. The dilution process used in the single-molecule LB SERRS work is independently supported by fluorescence results obtained from very dilute solutions with monomer concentrations down to 10−12 M. |
| doi_str_mv | 10.1366/000370203322005337 |
| format | Article |
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L. ; Lemma, Tibebe ; Antunes, Patricia A. ; Goulet, Paul ; Aroca, Ricardo</creator><creatorcontrib>Constantino, Carlos J. L. ; Lemma, Tibebe ; Antunes, Patricia A. ; Goulet, Paul ; Aroca, Ricardo</creatorcontrib><description>Surface-enhanced resonance Raman scattering (SERRS) is used for single-molecule detection from spatially resolved 1-μm2 sections of a Langmuir–Blodgett (LB) monolayer deposited onto a Ag film. The target molecule, bis (benzimidazo) thioperylene (BZP), is dispersed in an arachidic acid monomolecular layer containing one BZP molecule per μm2, which is also the probing area of the Raman microscope. For concentrated samples (attomole quantities in the field of view), average SERRS, surface-enhanced fluorescence (SEF), and Raman imaging, including line mapping and global images at different temperatures, were recorded. Single-molecule SERRS spectra, obtained using an LB monolayer, present changes in bandwidth and relative intensities, highlighting the properties of single-molecule SERRS that are lost in average SERRS measurements of mixed LB monolayers obtained at the same temperatures. Also, the dilute system phenomenon of blinking is discussed with regard to results obtained from LB monolayers. 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L.</creatorcontrib><creatorcontrib>Lemma, Tibebe</creatorcontrib><creatorcontrib>Antunes, Patricia A.</creatorcontrib><creatorcontrib>Goulet, Paul</creatorcontrib><creatorcontrib>Aroca, Ricardo</creatorcontrib><title>Surface-Enhanced Resonance Raman Scattering: Single-Molecule Detection in a Langmuir—Blodgett Monolayer</title><title>Applied spectroscopy</title><addtitle>Appl Spectrosc</addtitle><description>Surface-enhanced resonance Raman scattering (SERRS) is used for single-molecule detection from spatially resolved 1-μm2 sections of a Langmuir–Blodgett (LB) monolayer deposited onto a Ag film. The target molecule, bis (benzimidazo) thioperylene (BZP), is dispersed in an arachidic acid monomolecular layer containing one BZP molecule per μm2, which is also the probing area of the Raman microscope. For concentrated samples (attomole quantities in the field of view), average SERRS, surface-enhanced fluorescence (SEF), and Raman imaging, including line mapping and global images at different temperatures, were recorded. Single-molecule SERRS spectra, obtained using an LB monolayer, present changes in bandwidth and relative intensities, highlighting the properties of single-molecule SERRS that are lost in average SERRS measurements of mixed LB monolayers obtained at the same temperatures. Also, the dilute system phenomenon of blinking is discussed with regard to results obtained from LB monolayers. The dilution process used in the single-molecule LB SERRS work is independently supported by fluorescence results obtained from very dilute solutions with monomer concentrations down to 10−12 M.</description><subject>Benzimidazoles - analysis</subject><subject>Benzimidazoles - chemistry</subject><subject>Eicosanoic Acids - analysis</subject><subject>Eicosanoic Acids - chemistry</subject><subject>Macromolecular Substances</subject><subject>Membrane Lipids - analysis</subject><subject>Membrane Lipids - chemistry</subject><subject>Membranes, Artificial</subject><subject>Microchemistry - methods</subject><subject>Nanotechnology - methods</subject><subject>Perylene - analogs & derivatives</subject><subject>Scattering, Radiation</subject><subject>Silver - chemistry</subject><subject>Spectrometry, Fluorescence</subject><subject>Spectrum Analysis, Raman - methods</subject><subject>Surface Properties</subject><issn>0003-7028</issn><issn>1943-3530</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtOwzAQhi0EoqVwARbIK3ahdhznwQ5KeUitkFpYR1NnHFIlNtjJojsOwQk5CalaiQUSm3lI3_zSfIScc3bFRRyPGWMiYSETIgwZk0IkB2TIs0gEQgp2SIZbIOiJdEBOvF_3q8yEPCYDHsUyjbNkSKpl5zQoDKbmDYzCgi7QW7Md6QIaMHSpoG3RVaa8psu-1hjMbY2qq5HeYYuqrayhlaFAZ2DKpqvc9-fXbW2LEtuWzq2xNWzQnZIjDbXHs30fkdf76cvkMZg9PzxNbmaBEiJrg1RnMlwJHnFgMSDqVEYRS2SkUYIsIGYrjQJTAB5LnWpeQMF5VMhM8lj1Lkbkcpf77uxHh77Nm8orrGswaDufJzwKUy6yHgx3oHLWe4c6f3dVA26Tc5ZvBed_BfdHF_v0btVg8XuyN9oD4x3gocR8bTtn-m__i_wBENWEhg</recordid><startdate>200306</startdate><enddate>200306</enddate><creator>Constantino, Carlos J. 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L. ; Lemma, Tibebe ; Antunes, Patricia A. ; Goulet, Paul ; Aroca, Ricardo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c339t-8f952b3141a06aeef85440754fe5a5da60bfe3e8aa165f8f1dad114d59516c033</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Benzimidazoles - analysis</topic><topic>Benzimidazoles - chemistry</topic><topic>Eicosanoic Acids - analysis</topic><topic>Eicosanoic Acids - chemistry</topic><topic>Macromolecular Substances</topic><topic>Membrane Lipids - analysis</topic><topic>Membrane Lipids - chemistry</topic><topic>Membranes, Artificial</topic><topic>Microchemistry - methods</topic><topic>Nanotechnology - methods</topic><topic>Perylene - analogs & derivatives</topic><topic>Scattering, Radiation</topic><topic>Silver - chemistry</topic><topic>Spectrometry, Fluorescence</topic><topic>Spectrum Analysis, Raman - methods</topic><topic>Surface Properties</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Constantino, Carlos J. L.</creatorcontrib><creatorcontrib>Lemma, Tibebe</creatorcontrib><creatorcontrib>Antunes, Patricia A.</creatorcontrib><creatorcontrib>Goulet, Paul</creatorcontrib><creatorcontrib>Aroca, Ricardo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Applied spectroscopy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Constantino, Carlos J. L.</au><au>Lemma, Tibebe</au><au>Antunes, Patricia A.</au><au>Goulet, Paul</au><au>Aroca, Ricardo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Surface-Enhanced Resonance Raman Scattering: Single-Molecule Detection in a Langmuir—Blodgett Monolayer</atitle><jtitle>Applied spectroscopy</jtitle><addtitle>Appl Spectrosc</addtitle><date>2003-06</date><risdate>2003</risdate><volume>57</volume><issue>6</issue><spage>649</spage><epage>654</epage><pages>649-654</pages><issn>0003-7028</issn><eissn>1943-3530</eissn><abstract>Surface-enhanced resonance Raman scattering (SERRS) is used for single-molecule detection from spatially resolved 1-μm2 sections of a Langmuir–Blodgett (LB) monolayer deposited onto a Ag film. The target molecule, bis (benzimidazo) thioperylene (BZP), is dispersed in an arachidic acid monomolecular layer containing one BZP molecule per μm2, which is also the probing area of the Raman microscope. For concentrated samples (attomole quantities in the field of view), average SERRS, surface-enhanced fluorescence (SEF), and Raman imaging, including line mapping and global images at different temperatures, were recorded. Single-molecule SERRS spectra, obtained using an LB monolayer, present changes in bandwidth and relative intensities, highlighting the properties of single-molecule SERRS that are lost in average SERRS measurements of mixed LB monolayers obtained at the same temperatures. Also, the dilute system phenomenon of blinking is discussed with regard to results obtained from LB monolayers. The dilution process used in the single-molecule LB SERRS work is independently supported by fluorescence results obtained from very dilute solutions with monomer concentrations down to 10−12 M.</abstract><cop>London, England</cop><pub>SAGE Publications</pub><pmid>14658697</pmid><doi>10.1366/000370203322005337</doi><tpages>6</tpages></addata></record> |
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| subjects | Benzimidazoles - analysis Benzimidazoles - chemistry Eicosanoic Acids - analysis Eicosanoic Acids - chemistry Macromolecular Substances Membrane Lipids - analysis Membrane Lipids - chemistry Membranes, Artificial Microchemistry - methods Nanotechnology - methods Perylene - analogs & derivatives Scattering, Radiation Silver - chemistry Spectrometry, Fluorescence Spectrum Analysis, Raman - methods Surface Properties |
| title | Surface-Enhanced Resonance Raman Scattering: Single-Molecule Detection in a Langmuir—Blodgett Monolayer |
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