Primary structure and transcription analysis of a laccase-encoding gene from the basidiomycete Trametes trogii
A cDNA coding for laccase was isolated from the white-rot fungus Trametes trogii 201. This cDNA corresponded to the lcc1 gene, which coded for a precursor protein of 517 amino acids with a 21 amino acid signal peptide. Comparison of the deduced sequence with known laccases showed that this enzyme wa...
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| Veröffentlicht in: | Applied microbiology and biotechnology 2003-12, Vol.63 (2), p.153-158 |
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| creator | COLAO, M. Ch GARZILLO, A. M BUONOCORE, V SCHIESSER, A RUZZI, M |
| description | A cDNA coding for laccase was isolated from the white-rot fungus Trametes trogii 201. This cDNA corresponded to the lcc1 gene, which coded for a precursor protein of 517 amino acids with a 21 amino acid signal peptide. Comparison of the deduced sequence with known laccases showed that this enzyme was most closely related to Lac1 from basidiomycete PM1 and Trametes C30 (98% similarity). The expression of lcc1 was analysed under different growth conditions; transcription of this gene was enhanced by the addition of organic nitrogen to the medium. The level of lcc1 transcription was higher when T. trogii was grown on synthetic medium supplemented with yeast extract rather than mycological peptone or tryptone. The transcription data were in agreement with total laccase activity measured in the supernatant and suggested that laccase production and lcc1 transcription are coordinately regulated in this organism. The lcc1 cDNA was expressed in the methylotrophic yeast Pichia pastoris and the detection of laccase activity indicated that this cDNA encodes a laccase. |
| doi_str_mv | 10.1007/s00253-003-1429-x |
| format | Article |
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Ch ; GARZILLO, A. M ; BUONOCORE, V ; SCHIESSER, A ; RUZZI, M</creator><creatorcontrib>COLAO, M. Ch ; GARZILLO, A. M ; BUONOCORE, V ; SCHIESSER, A ; RUZZI, M</creatorcontrib><description>A cDNA coding for laccase was isolated from the white-rot fungus Trametes trogii 201. This cDNA corresponded to the lcc1 gene, which coded for a precursor protein of 517 amino acids with a 21 amino acid signal peptide. Comparison of the deduced sequence with known laccases showed that this enzyme was most closely related to Lac1 from basidiomycete PM1 and Trametes C30 (98% similarity). The expression of lcc1 was analysed under different growth conditions; transcription of this gene was enhanced by the addition of organic nitrogen to the medium. The level of lcc1 transcription was higher when T. trogii was grown on synthetic medium supplemented with yeast extract rather than mycological peptone or tryptone. The transcription data were in agreement with total laccase activity measured in the supernatant and suggested that laccase production and lcc1 transcription are coordinately regulated in this organism. The lcc1 cDNA was expressed in the methylotrophic yeast Pichia pastoris and the detection of laccase activity indicated that this cDNA encodes a laccase.</description><identifier>ISSN: 0175-7598</identifier><identifier>EISSN: 1432-0614</identifier><identifier>DOI: 10.1007/s00253-003-1429-x</identifier><identifier>PMID: 13680201</identifier><identifier>CODEN: AMBIDG</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Amino acids ; Basidiomycota - enzymology ; Basidiomycota - genetics ; Basidiomycota - growth & development ; Biological and medical sciences ; Biology of microorganisms of confirmed or potential industrial interest ; Biotechnology ; Cloning, Molecular ; Culture Media ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Fungal ; Genetics ; Growth conditions ; Laccase - chemistry ; Laccase - genetics ; Laccase - metabolism ; Microbiology ; Mission oriented research ; Molecular Sequence Data ; Organic nitrogen ; Peptones ; Pichia - enzymology ; Pichia - genetics ; Sequence Analysis, DNA ; Trametes trogii ; Transcription, Genetic ; Yeasts</subject><ispartof>Applied microbiology and biotechnology, 2003-12, Vol.63 (2), p.153-158</ispartof><rights>2004 INIST-CNRS</rights><rights>Springer-Verlag 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c422t-320d1aa79c1a053a85b56d94a50a7b597ad33ffb3226fa604fa7dc4c1c91761e3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27911,27912</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15512255$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/13680201$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>COLAO, M. Ch</creatorcontrib><creatorcontrib>GARZILLO, A. M</creatorcontrib><creatorcontrib>BUONOCORE, V</creatorcontrib><creatorcontrib>SCHIESSER, A</creatorcontrib><creatorcontrib>RUZZI, M</creatorcontrib><title>Primary structure and transcription analysis of a laccase-encoding gene from the basidiomycete Trametes trogii</title><title>Applied microbiology and biotechnology</title><addtitle>Appl Microbiol Biotechnol</addtitle><description>A cDNA coding for laccase was isolated from the white-rot fungus Trametes trogii 201. This cDNA corresponded to the lcc1 gene, which coded for a precursor protein of 517 amino acids with a 21 amino acid signal peptide. Comparison of the deduced sequence with known laccases showed that this enzyme was most closely related to Lac1 from basidiomycete PM1 and Trametes C30 (98% similarity). The expression of lcc1 was analysed under different growth conditions; transcription of this gene was enhanced by the addition of organic nitrogen to the medium. The level of lcc1 transcription was higher when T. trogii was grown on synthetic medium supplemented with yeast extract rather than mycological peptone or tryptone. The transcription data were in agreement with total laccase activity measured in the supernatant and suggested that laccase production and lcc1 transcription are coordinately regulated in this organism. The lcc1 cDNA was expressed in the methylotrophic yeast Pichia pastoris and the detection of laccase activity indicated that this cDNA encodes a laccase.</description><subject>Amino acids</subject><subject>Basidiomycota - enzymology</subject><subject>Basidiomycota - genetics</subject><subject>Basidiomycota - growth & development</subject><subject>Biological and medical sciences</subject><subject>Biology of microorganisms of confirmed or potential industrial interest</subject><subject>Biotechnology</subject><subject>Cloning, Molecular</subject><subject>Culture Media</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Fungal</subject><subject>Genetics</subject><subject>Growth conditions</subject><subject>Laccase - chemistry</subject><subject>Laccase - genetics</subject><subject>Laccase - metabolism</subject><subject>Microbiology</subject><subject>Mission oriented research</subject><subject>Molecular Sequence Data</subject><subject>Organic nitrogen</subject><subject>Peptones</subject><subject>Pichia - enzymology</subject><subject>Pichia - genetics</subject><subject>Sequence Analysis, DNA</subject><subject>Trametes trogii</subject><subject>Transcription, Genetic</subject><subject>Yeasts</subject><issn>0175-7598</issn><issn>1432-0614</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkU1r3DAQhkVpaTZpf0AvRRSam9sZyZLtYwn9gkB7SM9iLMtbBdvaamzI_vsq7EKgl8LAwPDMe3gfId4gfECA5iMDKKMrAF1hrbrq4ZnYYa1VBRbr52IH2JiqMV17IS6Z7wFQtda-FBeobQsKcCeWnznOlI-S17z5dctB0jLINdPCPsfDGtNSLjQdObJMoyQ5kffEoQqLT0Nc9nIfliDHnGa5_g6yJ45DTPPRhzXIu0xz2VwS0z7GV-LFSBOH1-d9JX59-Xx38626_fH1-82n28rXSq2VVjAgUdN5JDCaWtMbO3Q1GaCmN11Dg9bj2Gul7EgW6pGawdcefYeNxaCvxPUp95DTny3w6ubIPkwTLSFt7JrSly3zXxA7pQ3WbQHf_QPepy2XYthZhRYRWlUgPEE-J-YcRnc41esQ3KMyd1LmijL3qMw9lJ-35-Ctn8Pw9HF2VID3Z4DY0zQWNT7yE2cMKmWM_gsoYZ-o</recordid><startdate>20031201</startdate><enddate>20031201</enddate><creator>COLAO, M. 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Psychology</topic><topic>Gene Expression Regulation, Fungal</topic><topic>Genetics</topic><topic>Growth conditions</topic><topic>Laccase - chemistry</topic><topic>Laccase - genetics</topic><topic>Laccase - metabolism</topic><topic>Microbiology</topic><topic>Mission oriented research</topic><topic>Molecular Sequence Data</topic><topic>Organic nitrogen</topic><topic>Peptones</topic><topic>Pichia - enzymology</topic><topic>Pichia - genetics</topic><topic>Sequence Analysis, DNA</topic><topic>Trametes trogii</topic><topic>Transcription, Genetic</topic><topic>Yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>COLAO, M. Ch</creatorcontrib><creatorcontrib>GARZILLO, A. 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Ch</au><au>GARZILLO, A. M</au><au>BUONOCORE, V</au><au>SCHIESSER, A</au><au>RUZZI, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Primary structure and transcription analysis of a laccase-encoding gene from the basidiomycete Trametes trogii</atitle><jtitle>Applied microbiology and biotechnology</jtitle><addtitle>Appl Microbiol Biotechnol</addtitle><date>2003-12-01</date><risdate>2003</risdate><volume>63</volume><issue>2</issue><spage>153</spage><epage>158</epage><pages>153-158</pages><issn>0175-7598</issn><eissn>1432-0614</eissn><coden>AMBIDG</coden><abstract>A cDNA coding for laccase was isolated from the white-rot fungus Trametes trogii 201. This cDNA corresponded to the lcc1 gene, which coded for a precursor protein of 517 amino acids with a 21 amino acid signal peptide. Comparison of the deduced sequence with known laccases showed that this enzyme was most closely related to Lac1 from basidiomycete PM1 and Trametes C30 (98% similarity). The expression of lcc1 was analysed under different growth conditions; transcription of this gene was enhanced by the addition of organic nitrogen to the medium. The level of lcc1 transcription was higher when T. trogii was grown on synthetic medium supplemented with yeast extract rather than mycological peptone or tryptone. The transcription data were in agreement with total laccase activity measured in the supernatant and suggested that laccase production and lcc1 transcription are coordinately regulated in this organism. The lcc1 cDNA was expressed in the methylotrophic yeast Pichia pastoris and the detection of laccase activity indicated that this cDNA encodes a laccase.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>13680201</pmid><doi>10.1007/s00253-003-1429-x</doi><tpages>6</tpages></addata></record> |
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| ispartof | Applied microbiology and biotechnology, 2003-12, Vol.63 (2), p.153-158 |
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| subjects | Amino acids Basidiomycota - enzymology Basidiomycota - genetics Basidiomycota - growth & development Biological and medical sciences Biology of microorganisms of confirmed or potential industrial interest Biotechnology Cloning, Molecular Culture Media Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Fungal Genetics Growth conditions Laccase - chemistry Laccase - genetics Laccase - metabolism Microbiology Mission oriented research Molecular Sequence Data Organic nitrogen Peptones Pichia - enzymology Pichia - genetics Sequence Analysis, DNA Trametes trogii Transcription, Genetic Yeasts |
| title | Primary structure and transcription analysis of a laccase-encoding gene from the basidiomycete Trametes trogii |
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