A Rapid Method to Capture and Screen for Transcription Factors by SELDI Mass Spectrometry

A novel method to screen for transcription factors binding to promoter DNA sequences has been developed using DNA chip surfaces and mass spectrometry. This technique was demonstrated with Escherichia coli lac repressor, LacI. The consensus promoter binding sequence for LacI and a scrambled version o...

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Veröffentlicht in:	Biochemical and biophysical research communications 2002-02, Vol.290 (4), p.1328-1335
Hauptverfasser:	Forde, Cameron E., Gonzales, Arlene D., Smessaert, Jean M., Murphy, Gloria A., Shields, Sharon J., Fitch, J.Patrick, McCutchen-Maloney, Sandra L.
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial Proteins - genetics Bacterial Proteins - isolation & purification Bacterial Proteins - metabolism Base Sequence Binding Sites - genetics DNA, Bacterial - genetics DNA, Bacterial - metabolism Escherichia coli Escherichia coli - genetics Escherichia coli - metabolism Escherichia coli Proteins Lac repressor Lac Repressors mass spectrometry Oligonucleotide Array Sequence Analysis Peptide Mapping Promoter Regions, Genetic proteomics regulatory protein Repressor Proteins - genetics Repressor Proteins - isolation & purification Repressor Proteins - metabolism SELDI Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods transcription factor Transcription Factors - genetics Transcription Factors - isolation & purification Transcription Factors - metabolism
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