A comparative evaluation of dot immunobinding assay (Dot-Iba) and polymerase chain reaction (PCR) for the laboratory diagnosis of tuberculous meningitis
The results of a Dot immunobinding assay (Dot Iba) for the detection of mycobacterial antigen in the cerebrospinal fluid (CSF) of 45 patients with tuberculous meningitis (TBM) were compared with the results of a polymerase chain reaction (PCR) for the detection of Mycobacterium tuberculosis. In eigh...
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| Veröffentlicht in: | Diagnostic microbiology and infectious disease 2002, Vol.42 (1), p.35-38 |
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| container_title | Diagnostic microbiology and infectious disease |
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| creator | Sumi, M.G Mathai, A Reuben, S Sarada, C Radhakrishnan, V.V Indulakshmi, R Sathish, M Ajaykumar, R Manju, Y.K |
| description | The results of a Dot immunobinding assay (Dot Iba) for the detection of mycobacterial antigen in the cerebrospinal fluid (CSF) of 45 patients with tuberculous meningitis (TBM) were compared with the results of a polymerase chain reaction (PCR) for the detection of
Mycobacterium tuberculosis. In eight patients with culture proven TBM, Dot-Iba gave positive results, while PCR yielded positive results only in six patients. The overall sensitivities of Dot-Iba and PCR in 37 patients with culture negative (probable) TBM were 75.67% and 40.5% respectively. Dot-Iba, in contrast to PCR is a rapid and relatively easier method. More importantly, Dot-Iba is suitable for the routine application for the laboratory diagnosis of TBM and therefore best suited to laboratories in the developing world. |
| doi_str_mv | 10.1016/S0732-8893(01)00342-X |
| format | Article |
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Mycobacterium tuberculosis. In eight patients with culture proven TBM, Dot-Iba gave positive results, while PCR yielded positive results only in six patients. The overall sensitivities of Dot-Iba and PCR in 37 patients with culture negative (probable) TBM were 75.67% and 40.5% respectively. Dot-Iba, in contrast to PCR is a rapid and relatively easier method. More importantly, Dot-Iba is suitable for the routine application for the laboratory diagnosis of TBM and therefore best suited to laboratories in the developing world.</description><identifier>ISSN: 0732-8893</identifier><identifier>EISSN: 1879-0070</identifier><identifier>DOI: 10.1016/S0732-8893(01)00342-X</identifier><identifier>PMID: 11821169</identifier><identifier>CODEN: DMIDDZ</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Antigens, Bacterial - cerebrospinal fluid ; Bacteriological methods and techniques used in bacteriology ; Bacteriology ; Biological and medical sciences ; DNA, Bacterial - cerebrospinal fluid ; Dot immunobinding assay (Dot-Iba) ; Fundamental and applied biological sciences. Psychology ; Humans ; Immunoblotting - methods ; Laboratories ; Laboratory diagnosis ; Microbiology ; Mycobacterium tuberculosis ; Mycobacterium tuberculosis - genetics ; Mycobacterium tuberculosis - immunology ; Mycobacterium tuberculosis - isolation & purification ; Polymerase Chain Reaction - methods ; Polymerase chain reaction PCR ; Sensitivity and Specificity ; Techniques used in virology ; Tuberculosis, Meningeal - cerebrospinal fluid ; Tuberculosis, Meningeal - diagnosis ; Tuberculosis, Meningeal - immunology ; Tuberculosis, Meningeal - microbiology ; Tuberculous meningitis ; Virology</subject><ispartof>Diagnostic microbiology and infectious disease, 2002, Vol.42 (1), p.35-38</ispartof><rights>2002 Elsevier Science Inc.</rights><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c391t-f7c54582d3e71d4a7d8066bb20e51cb460510b7738117407a99b28bf477e84f83</citedby><cites>FETCH-LOGICAL-c391t-f7c54582d3e71d4a7d8066bb20e51cb460510b7738117407a99b28bf477e84f83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0732-8893(01)00342-X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3549,4023,27922,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13467579$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11821169$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sumi, M.G</creatorcontrib><creatorcontrib>Mathai, A</creatorcontrib><creatorcontrib>Reuben, S</creatorcontrib><creatorcontrib>Sarada, C</creatorcontrib><creatorcontrib>Radhakrishnan, V.V</creatorcontrib><creatorcontrib>Indulakshmi, R</creatorcontrib><creatorcontrib>Sathish, M</creatorcontrib><creatorcontrib>Ajaykumar, R</creatorcontrib><creatorcontrib>Manju, Y.K</creatorcontrib><title>A comparative evaluation of dot immunobinding assay (Dot-Iba) and polymerase chain reaction (PCR) for the laboratory diagnosis of tuberculous meningitis</title><title>Diagnostic microbiology and infectious disease</title><addtitle>Diagn Microbiol Infect Dis</addtitle><description>The results of a Dot immunobinding assay (Dot Iba) for the detection of mycobacterial antigen in the cerebrospinal fluid (CSF) of 45 patients with tuberculous meningitis (TBM) were compared with the results of a polymerase chain reaction (PCR) for the detection of
Mycobacterium tuberculosis. In eight patients with culture proven TBM, Dot-Iba gave positive results, while PCR yielded positive results only in six patients. The overall sensitivities of Dot-Iba and PCR in 37 patients with culture negative (probable) TBM were 75.67% and 40.5% respectively. Dot-Iba, in contrast to PCR is a rapid and relatively easier method. More importantly, Dot-Iba is suitable for the routine application for the laboratory diagnosis of TBM and therefore best suited to laboratories in the developing world.</description><subject>Antigens, Bacterial - cerebrospinal fluid</subject><subject>Bacteriological methods and techniques used in bacteriology</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>DNA, Bacterial - cerebrospinal fluid</subject><subject>Dot immunobinding assay (Dot-Iba)</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Immunoblotting - methods</subject><subject>Laboratories</subject><subject>Laboratory diagnosis</subject><subject>Microbiology</subject><subject>Mycobacterium tuberculosis</subject><subject>Mycobacterium tuberculosis - genetics</subject><subject>Mycobacterium tuberculosis - immunology</subject><subject>Mycobacterium tuberculosis - isolation & purification</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Polymerase chain reaction PCR</subject><subject>Sensitivity and Specificity</subject><subject>Techniques used in virology</subject><subject>Tuberculosis, Meningeal - cerebrospinal fluid</subject><subject>Tuberculosis, Meningeal - diagnosis</subject><subject>Tuberculosis, Meningeal - immunology</subject><subject>Tuberculosis, Meningeal - microbiology</subject><subject>Tuberculous meningitis</subject><subject>Virology</subject><issn>0732-8893</issn><issn>1879-0070</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtv1DAUhS0EokPhJ4C8Ac0sAr6JEzsrVA0tVKoE4iF1Z_lx0xol9tRORpp_ws8l8xBdsrp38d17js4h5DWw98Cg-fCDiaospGyrJYMVYxUvi9snZAFStAVjgj0li3_IGXmR82_GoGw5e07OAGQJ0LQL8ueC2jhsdNKj3yLFre6neY2Bxo66OFI_DFOIxgfnwx3VOesdXX6KY3Ft9Irq4Ogm9rsBk85I7b32gSbU9vBi-W39fUW7mOh4j7TXJs4yMe2o8_ouxOzzXmWcDCY79XHKdMAwy_jR55fkWaf7jK9O85z8urr8uf5S3Hz9fL2-uCls1cJYdMLWvJalq1CA41o4yZrGmJJhDdbwhtXAjBCVBBCcCd22ppSm40Kg5J2szsm7499Nig8T5lENPlvsex1wdqQEcJANwAzWR9CmmHPCTm2SH3TaKWBqX4k6VKL2eSsG6lCJup3v3pwEJjOge7w6dTADb0-Azlb3XdLB-vzIVbwRtdhzH48cznFsPSaVrcdg0fmEdlQu-v9Y-QvlJqoH</recordid><startdate>2002</startdate><enddate>2002</enddate><creator>Sumi, M.G</creator><creator>Mathai, A</creator><creator>Reuben, S</creator><creator>Sarada, C</creator><creator>Radhakrishnan, V.V</creator><creator>Indulakshmi, R</creator><creator>Sathish, M</creator><creator>Ajaykumar, R</creator><creator>Manju, Y.K</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2002</creationdate><title>A comparative evaluation of dot immunobinding assay (Dot-Iba) and polymerase chain reaction (PCR) for the laboratory diagnosis of tuberculous meningitis</title><author>Sumi, M.G ; Mathai, A ; Reuben, S ; Sarada, C ; Radhakrishnan, V.V ; Indulakshmi, R ; Sathish, M ; Ajaykumar, R ; Manju, Y.K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c391t-f7c54582d3e71d4a7d8066bb20e51cb460510b7738117407a99b28bf477e84f83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Antigens, Bacterial - cerebrospinal fluid</topic><topic>Bacteriological methods and techniques used in bacteriology</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>DNA, Bacterial - cerebrospinal fluid</topic><topic>Dot immunobinding assay (Dot-Iba)</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Immunoblotting - methods</topic><topic>Laboratories</topic><topic>Laboratory diagnosis</topic><topic>Microbiology</topic><topic>Mycobacterium tuberculosis</topic><topic>Mycobacterium tuberculosis - genetics</topic><topic>Mycobacterium tuberculosis - immunology</topic><topic>Mycobacterium tuberculosis - isolation & purification</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Polymerase chain reaction PCR</topic><topic>Sensitivity and Specificity</topic><topic>Techniques used in virology</topic><topic>Tuberculosis, Meningeal - cerebrospinal fluid</topic><topic>Tuberculosis, Meningeal - diagnosis</topic><topic>Tuberculosis, Meningeal - immunology</topic><topic>Tuberculosis, Meningeal - microbiology</topic><topic>Tuberculous meningitis</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sumi, M.G</creatorcontrib><creatorcontrib>Mathai, A</creatorcontrib><creatorcontrib>Reuben, S</creatorcontrib><creatorcontrib>Sarada, C</creatorcontrib><creatorcontrib>Radhakrishnan, V.V</creatorcontrib><creatorcontrib>Indulakshmi, R</creatorcontrib><creatorcontrib>Sathish, M</creatorcontrib><creatorcontrib>Ajaykumar, R</creatorcontrib><creatorcontrib>Manju, Y.K</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Diagnostic microbiology and infectious disease</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sumi, M.G</au><au>Mathai, A</au><au>Reuben, S</au><au>Sarada, C</au><au>Radhakrishnan, V.V</au><au>Indulakshmi, R</au><au>Sathish, M</au><au>Ajaykumar, R</au><au>Manju, Y.K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A comparative evaluation of dot immunobinding assay (Dot-Iba) and polymerase chain reaction (PCR) for the laboratory diagnosis of tuberculous meningitis</atitle><jtitle>Diagnostic microbiology and infectious disease</jtitle><addtitle>Diagn Microbiol Infect Dis</addtitle><date>2002</date><risdate>2002</risdate><volume>42</volume><issue>1</issue><spage>35</spage><epage>38</epage><pages>35-38</pages><issn>0732-8893</issn><eissn>1879-0070</eissn><coden>DMIDDZ</coden><abstract>The results of a Dot immunobinding assay (Dot Iba) for the detection of mycobacterial antigen in the cerebrospinal fluid (CSF) of 45 patients with tuberculous meningitis (TBM) were compared with the results of a polymerase chain reaction (PCR) for the detection of
Mycobacterium tuberculosis. In eight patients with culture proven TBM, Dot-Iba gave positive results, while PCR yielded positive results only in six patients. The overall sensitivities of Dot-Iba and PCR in 37 patients with culture negative (probable) TBM were 75.67% and 40.5% respectively. Dot-Iba, in contrast to PCR is a rapid and relatively easier method. More importantly, Dot-Iba is suitable for the routine application for the laboratory diagnosis of TBM and therefore best suited to laboratories in the developing world.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>11821169</pmid><doi>10.1016/S0732-8893(01)00342-X</doi><tpages>4</tpages></addata></record> |
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| subjects | Antigens, Bacterial - cerebrospinal fluid Bacteriological methods and techniques used in bacteriology Bacteriology Biological and medical sciences DNA, Bacterial - cerebrospinal fluid Dot immunobinding assay (Dot-Iba) Fundamental and applied biological sciences. Psychology Humans Immunoblotting - methods Laboratories Laboratory diagnosis Microbiology Mycobacterium tuberculosis Mycobacterium tuberculosis - genetics Mycobacterium tuberculosis - immunology Mycobacterium tuberculosis - isolation & purification Polymerase Chain Reaction - methods Polymerase chain reaction PCR Sensitivity and Specificity Techniques used in virology Tuberculosis, Meningeal - cerebrospinal fluid Tuberculosis, Meningeal - diagnosis Tuberculosis, Meningeal - immunology Tuberculosis, Meningeal - microbiology Tuberculous meningitis Virology |
| title | A comparative evaluation of dot immunobinding assay (Dot-Iba) and polymerase chain reaction (PCR) for the laboratory diagnosis of tuberculous meningitis |
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