Detection of protease activities with the mass-spectrometry-assisted enzyme-screening (MES) system

The MALDI-MES provides a rapid, sensitive and reproducible alternative approach to existing analytical techniques for the detection of enzymatic activities that does not require a chromophore or radiolabeling. An improved method is presented, by which enzymes with defined substrate specificities can...

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Veröffentlicht in:	Analytical and bioanalytical chemistry 2003-12, Vol.377 (7-8), p.1102-1107
Hauptverfasser:	Schlüter, Hartmut, Jankowski, Joachim, Rykl, Jana, Thiemann, Joachim, Belgardt, Swetlana, Zidek, Walter, Wittmann, Brigitte, Pohl, Thomas
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Endopeptidases - analysis Endopeptidases - metabolism Kidney - enzymology Models, Chemical Protein Array Analysis Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods Swine - metabolism
Online-Zugang:	Volltext
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