Two bipartite NLSs mediate constitutive nuclear localization of Mcm10
Some components of the pre-replicative complex (pre-RC) are in the nucleus only during the G1 phase, while others are constitutively nuclear throughout the cell cycle. A nuclear localization signal (NLS) mediating the G1-specific nuclear localization has been characterized. We report here the identi...
Gespeichert in:
| Veröffentlicht in: | Current genetics 2003-12, Vol.44 (4), p.195-201 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 201 |
|---|---|
| container_issue | 4 |
| container_start_page | 195 |
| container_title | Current genetics |
| container_volume | 44 |
| creator | Burich, Rebekah Lei, Ming |
| description | Some components of the pre-replicative complex (pre-RC) are in the nucleus only during the G1 phase, while others are constitutively nuclear throughout the cell cycle. A nuclear localization signal (NLS) mediating the G1-specific nuclear localization has been characterized. We report here the identification of bipartite NLSs in constitutively nuclear pre-RC components, including the origin recognition complex, Mcm10 and Noc3. Interestingly, in a subgroup of these pre-RC components, two putative NLSs were found in each protein. To examine whether multiple NLSs are required for these proteins to be nuclear throughout the cell cycle, we characterized the two NLSs in Mcm10. We show that at least one of the two NLSs is required for directing Mcm10 into the nucleus and that either of them is sufficient to bring the green fluorescence protein into the nucleus. Thus, Mcm10 contains two functional NLSs and either is sufficient for the constitutive nuclear localization of Mcm10. |
| doi_str_mv | 10.1007/s00294-003-0443-y |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_71415786</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>19221515</sourcerecordid><originalsourceid>FETCH-LOGICAL-c355t-e3da5817cb049bdf8e60e97e9f1a605a954a8ed7d4e0df30d8c45cab213a71673</originalsourceid><addsrcrecordid>eNqFkU9LxDAQxYMo7rr6AbxI8eCtOtMkTXIUWf_AqgfXc0jTFLq0zdq0yvrpzbILghdPwwy_92DeI-Qc4RoBxE0AyBRLAWgKjNF0c0CmyGiWgpL0kEwBRZZKkHRCTkJYAWAmlTgmE6S5BORiSubLL58U9dr0Qz245GXxFpLWlbWJi_VdiNdxqD9d0o22caZPGm9NU3-bofZd4qvk2bYIp-SoMk1wZ_s5I-_38-XdY7p4fXi6u12klnI-pI6WhksUtgCmirKSLgenhFMVmhy4UZwZ6UpRMgdlRaGUlnFrigypEZgLOiNXO9917z9GFwbd1sG6pjGd82PQAln8Sub_gqiyDDnyCF7-AVd-7Lv4hJYg8iympCKEO8j2PoTeVXrd163pNxpBb5vQuyZ0bEJvm9CbqLnYG49FDPRXsY-e_gBKQIND</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>807620159</pqid></control><display><type>article</type><title>Two bipartite NLSs mediate constitutive nuclear localization of Mcm10</title><source>MEDLINE</source><source>SpringerNature Journals</source><creator>Burich, Rebekah ; Lei, Ming</creator><creatorcontrib>Burich, Rebekah ; Lei, Ming</creatorcontrib><description>Some components of the pre-replicative complex (pre-RC) are in the nucleus only during the G1 phase, while others are constitutively nuclear throughout the cell cycle. A nuclear localization signal (NLS) mediating the G1-specific nuclear localization has been characterized. We report here the identification of bipartite NLSs in constitutively nuclear pre-RC components, including the origin recognition complex, Mcm10 and Noc3. Interestingly, in a subgroup of these pre-RC components, two putative NLSs were found in each protein. To examine whether multiple NLSs are required for these proteins to be nuclear throughout the cell cycle, we characterized the two NLSs in Mcm10. We show that at least one of the two NLSs is required for directing Mcm10 into the nucleus and that either of them is sufficient to bring the green fluorescence protein into the nucleus. Thus, Mcm10 contains two functional NLSs and either is sufficient for the constitutive nuclear localization of Mcm10.</description><identifier>ISSN: 0172-8083</identifier><identifier>EISSN: 1432-0983</identifier><identifier>DOI: 10.1007/s00294-003-0443-y</identifier><identifier>PMID: 13680157</identifier><language>eng</language><publisher>United States: Springer Nature B.V</publisher><subject>Blotting, Western ; Carrier Proteins - genetics ; Carrier Proteins - metabolism ; Cell cycle ; Cell Cycle Proteins - genetics ; Cell Cycle Proteins - metabolism ; Cell Nucleus - metabolism ; Cell Nucleus - physiology ; Chromosomal Proteins, Non-Histone ; DNA-Binding Proteins - genetics ; DNA-Binding Proteins - metabolism ; G1 Phase - physiology ; Green Fluorescent Proteins ; Indoles ; Luminescent Proteins - metabolism ; Mcm10 protein ; Microscopy, Fluorescence ; Minichromosome Maintenance Proteins ; Noc3 protein ; Nuclear Localization Signals - metabolism ; Nuclear Proteins - genetics ; Nuclear Proteins - metabolism ; Nucleocytoplasmic Transport Proteins ; Origin Recognition Complex ; Plasmids - genetics ; Plasmids - metabolism ; Saccharomyces cerevisiae - cytology ; Saccharomyces cerevisiae Proteins - genetics ; Saccharomyces cerevisiae Proteins - metabolism</subject><ispartof>Current genetics, 2003-12, Vol.44 (4), p.195-201</ispartof><rights>Springer-Verlag 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c355t-e3da5817cb049bdf8e60e97e9f1a605a954a8ed7d4e0df30d8c45cab213a71673</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/13680157$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Burich, Rebekah</creatorcontrib><creatorcontrib>Lei, Ming</creatorcontrib><title>Two bipartite NLSs mediate constitutive nuclear localization of Mcm10</title><title>Current genetics</title><addtitle>Curr Genet</addtitle><description>Some components of the pre-replicative complex (pre-RC) are in the nucleus only during the G1 phase, while others are constitutively nuclear throughout the cell cycle. A nuclear localization signal (NLS) mediating the G1-specific nuclear localization has been characterized. We report here the identification of bipartite NLSs in constitutively nuclear pre-RC components, including the origin recognition complex, Mcm10 and Noc3. Interestingly, in a subgroup of these pre-RC components, two putative NLSs were found in each protein. To examine whether multiple NLSs are required for these proteins to be nuclear throughout the cell cycle, we characterized the two NLSs in Mcm10. We show that at least one of the two NLSs is required for directing Mcm10 into the nucleus and that either of them is sufficient to bring the green fluorescence protein into the nucleus. Thus, Mcm10 contains two functional NLSs and either is sufficient for the constitutive nuclear localization of Mcm10.</description><subject>Blotting, Western</subject><subject>Carrier Proteins - genetics</subject><subject>Carrier Proteins - metabolism</subject><subject>Cell cycle</subject><subject>Cell Cycle Proteins - genetics</subject><subject>Cell Cycle Proteins - metabolism</subject><subject>Cell Nucleus - metabolism</subject><subject>Cell Nucleus - physiology</subject><subject>Chromosomal Proteins, Non-Histone</subject><subject>DNA-Binding Proteins - genetics</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>G1 Phase - physiology</subject><subject>Green Fluorescent Proteins</subject><subject>Indoles</subject><subject>Luminescent Proteins - metabolism</subject><subject>Mcm10 protein</subject><subject>Microscopy, Fluorescence</subject><subject>Minichromosome Maintenance Proteins</subject><subject>Noc3 protein</subject><subject>Nuclear Localization Signals - metabolism</subject><subject>Nuclear Proteins - genetics</subject><subject>Nuclear Proteins - metabolism</subject><subject>Nucleocytoplasmic Transport Proteins</subject><subject>Origin Recognition Complex</subject><subject>Plasmids - genetics</subject><subject>Plasmids - metabolism</subject><subject>Saccharomyces cerevisiae - cytology</subject><subject>Saccharomyces cerevisiae Proteins - genetics</subject><subject>Saccharomyces cerevisiae Proteins - metabolism</subject><issn>0172-8083</issn><issn>1432-0983</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkU9LxDAQxYMo7rr6AbxI8eCtOtMkTXIUWf_AqgfXc0jTFLq0zdq0yvrpzbILghdPwwy_92DeI-Qc4RoBxE0AyBRLAWgKjNF0c0CmyGiWgpL0kEwBRZZKkHRCTkJYAWAmlTgmE6S5BORiSubLL58U9dr0Qz245GXxFpLWlbWJi_VdiNdxqD9d0o22caZPGm9NU3-bofZd4qvk2bYIp-SoMk1wZ_s5I-_38-XdY7p4fXi6u12klnI-pI6WhksUtgCmirKSLgenhFMVmhy4UZwZ6UpRMgdlRaGUlnFrigypEZgLOiNXO9917z9GFwbd1sG6pjGd82PQAln8Sub_gqiyDDnyCF7-AVd-7Lv4hJYg8iympCKEO8j2PoTeVXrd163pNxpBb5vQuyZ0bEJvm9CbqLnYG49FDPRXsY-e_gBKQIND</recordid><startdate>200312</startdate><enddate>200312</enddate><creator>Burich, Rebekah</creator><creator>Lei, Ming</creator><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7SS</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200312</creationdate><title>Two bipartite NLSs mediate constitutive nuclear localization of Mcm10</title><author>Burich, Rebekah ; Lei, Ming</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c355t-e3da5817cb049bdf8e60e97e9f1a605a954a8ed7d4e0df30d8c45cab213a71673</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Blotting, Western</topic><topic>Carrier Proteins - genetics</topic><topic>Carrier Proteins - metabolism</topic><topic>Cell cycle</topic><topic>Cell Cycle Proteins - genetics</topic><topic>Cell Cycle Proteins - metabolism</topic><topic>Cell Nucleus - metabolism</topic><topic>Cell Nucleus - physiology</topic><topic>Chromosomal Proteins, Non-Histone</topic><topic>DNA-Binding Proteins - genetics</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>G1 Phase - physiology</topic><topic>Green Fluorescent Proteins</topic><topic>Indoles</topic><topic>Luminescent Proteins - metabolism</topic><topic>Mcm10 protein</topic><topic>Microscopy, Fluorescence</topic><topic>Minichromosome Maintenance Proteins</topic><topic>Noc3 protein</topic><topic>Nuclear Localization Signals - metabolism</topic><topic>Nuclear Proteins - genetics</topic><topic>Nuclear Proteins - metabolism</topic><topic>Nucleocytoplasmic Transport Proteins</topic><topic>Origin Recognition Complex</topic><topic>Plasmids - genetics</topic><topic>Plasmids - metabolism</topic><topic>Saccharomyces cerevisiae - cytology</topic><topic>Saccharomyces cerevisiae Proteins - genetics</topic><topic>Saccharomyces cerevisiae Proteins - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Burich, Rebekah</creatorcontrib><creatorcontrib>Lei, Ming</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biological Sciences</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Current genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Burich, Rebekah</au><au>Lei, Ming</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Two bipartite NLSs mediate constitutive nuclear localization of Mcm10</atitle><jtitle>Current genetics</jtitle><addtitle>Curr Genet</addtitle><date>2003-12</date><risdate>2003</risdate><volume>44</volume><issue>4</issue><spage>195</spage><epage>201</epage><pages>195-201</pages><issn>0172-8083</issn><eissn>1432-0983</eissn><abstract>Some components of the pre-replicative complex (pre-RC) are in the nucleus only during the G1 phase, while others are constitutively nuclear throughout the cell cycle. A nuclear localization signal (NLS) mediating the G1-specific nuclear localization has been characterized. We report here the identification of bipartite NLSs in constitutively nuclear pre-RC components, including the origin recognition complex, Mcm10 and Noc3. Interestingly, in a subgroup of these pre-RC components, two putative NLSs were found in each protein. To examine whether multiple NLSs are required for these proteins to be nuclear throughout the cell cycle, we characterized the two NLSs in Mcm10. We show that at least one of the two NLSs is required for directing Mcm10 into the nucleus and that either of them is sufficient to bring the green fluorescence protein into the nucleus. Thus, Mcm10 contains two functional NLSs and either is sufficient for the constitutive nuclear localization of Mcm10.</abstract><cop>United States</cop><pub>Springer Nature B.V</pub><pmid>13680157</pmid><doi>10.1007/s00294-003-0443-y</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0172-8083 |
| ispartof | Current genetics, 2003-12, Vol.44 (4), p.195-201 |
| issn | 0172-8083 1432-0983 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_71415786 |
| source | MEDLINE; SpringerNature Journals |
| subjects | Blotting, Western Carrier Proteins - genetics Carrier Proteins - metabolism Cell cycle Cell Cycle Proteins - genetics Cell Cycle Proteins - metabolism Cell Nucleus - metabolism Cell Nucleus - physiology Chromosomal Proteins, Non-Histone DNA-Binding Proteins - genetics DNA-Binding Proteins - metabolism G1 Phase - physiology Green Fluorescent Proteins Indoles Luminescent Proteins - metabolism Mcm10 protein Microscopy, Fluorescence Minichromosome Maintenance Proteins Noc3 protein Nuclear Localization Signals - metabolism Nuclear Proteins - genetics Nuclear Proteins - metabolism Nucleocytoplasmic Transport Proteins Origin Recognition Complex Plasmids - genetics Plasmids - metabolism Saccharomyces cerevisiae - cytology Saccharomyces cerevisiae Proteins - genetics Saccharomyces cerevisiae Proteins - metabolism |
| title | Two bipartite NLSs mediate constitutive nuclear localization of Mcm10 |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-03T19%3A27%3A52IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Two%20bipartite%20NLSs%20mediate%20constitutive%20nuclear%20localization%20of%20Mcm10&rft.jtitle=Current%20genetics&rft.au=Burich,%20Rebekah&rft.date=2003-12&rft.volume=44&rft.issue=4&rft.spage=195&rft.epage=201&rft.pages=195-201&rft.issn=0172-8083&rft.eissn=1432-0983&rft_id=info:doi/10.1007/s00294-003-0443-y&rft_dat=%3Cproquest_cross%3E19221515%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=807620159&rft_id=info:pmid/13680157&rfr_iscdi=true |