Macromolecular structure of cellulose studied by second-harmonic generation imaging microscopy
The macromolecular structure of purified cellulose samples is studied by second-harmonic generation (SHG) imaging microscopy. We show that the SHG contrast in both Valonia and Acetobacter cellulose strongly resembles that of collagen from animal tissues, both in terms of morphology and polarization...
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| Veröffentlicht in: | Optics letters 2003-11, Vol.28 (22), p.2207-2209 |
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| container_title | Optics letters |
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| creator | Brown, Jr, R Malcom Millard, Andrew C Campagnola, Paul J |
| description | The macromolecular structure of purified cellulose samples is studied by second-harmonic generation (SHG) imaging microscopy. We show that the SHG contrast in both Valonia and Acetobacter cellulose strongly resembles that of collagen from animal tissues, both in terms of morphology and polarization anisotropy. Polarization analysis shows that microfibrils in each lamella are highly aligned and ordered and change directions by 90 degrees in adjacent lamellae. The angular dependence of the SHG intensity fits well to a cos2 theta distribution, which is characteristic of the electric dipole interaction. Enzymatic degradation of Valonia fibers by cellulase is followed in real time by SHG imaging and results in exponential decay kinetics, showing that SHG imaging microscopy is ideal for monitoring dynamics in biological systems. |
| doi_str_mv | 10.1364/ol.28.002207 |
| format | Article |
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We show that the SHG contrast in both Valonia and Acetobacter cellulose strongly resembles that of collagen from animal tissues, both in terms of morphology and polarization anisotropy. Polarization analysis shows that microfibrils in each lamella are highly aligned and ordered and change directions by 90 degrees in adjacent lamellae. The angular dependence of the SHG intensity fits well to a cos2 theta distribution, which is characteristic of the electric dipole interaction. Enzymatic degradation of Valonia fibers by cellulase is followed in real time by SHG imaging and results in exponential decay kinetics, showing that SHG imaging microscopy is ideal for monitoring dynamics in biological systems.</description><subject>Acetobacter - chemistry</subject><subject>Anisotropy</subject><subject>Cellulose - chemistry</subject><subject>Chlorophyta - chemistry</subject><subject>Diagnostic Imaging</subject><subject>Macromolecular Substances</subject><subject>Microscopy, Confocal</subject><subject>Microscopy, Polarization</subject><subject>Molecular Structure</subject><subject>Optics and Photonics</subject><issn>0146-9592</issn><issn>1539-4794</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkE1LAzEURYMotlZ3riUrV05N8tKkWYr4BZVudGvIZN7UkZlJTSaL_nuntODqwuVwuFxCrjmbc1DyPrRzsZwzJgTTJ2TKF2AKqY08JVPGpSrMwogJuUjphzGmNMA5mYy9NEbClHy9Ox9DF1r0uXWRpiFmP-SINNTUY9vmNiQc61w1WNFyRxP60FfFt4td6BtPN9hjdEMTetp0btP0G9o1ozP5sN1dkrPatQmvjjkjn89PH4-vxWr98vb4sCo8LGAoZFmVZaXYUgkvkEmlBEcjPWpjHNRYcl07QC1kDWbhtQJmwAswrpSMVQJm5Pbg3cbwmzENtmvSfr7rMeRkNZcchNYjeHcA9wtTxNpu4zg77ixndv-nXa-sWNrDnyN-c_TmssPqHz4eCH-r73I1</recordid><startdate>20031115</startdate><enddate>20031115</enddate><creator>Brown, Jr, R Malcom</creator><creator>Millard, Andrew C</creator><creator>Campagnola, Paul J</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20031115</creationdate><title>Macromolecular structure of cellulose studied by second-harmonic generation imaging microscopy</title><author>Brown, Jr, R Malcom ; Millard, Andrew C ; Campagnola, Paul J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c353t-4bdbbd60862c2e046621e94ce799a3feb17fa3e724f395c763093c239ab400d23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Acetobacter - chemistry</topic><topic>Anisotropy</topic><topic>Cellulose - chemistry</topic><topic>Chlorophyta - chemistry</topic><topic>Diagnostic Imaging</topic><topic>Macromolecular Substances</topic><topic>Microscopy, Confocal</topic><topic>Microscopy, Polarization</topic><topic>Molecular Structure</topic><topic>Optics and Photonics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Brown, Jr, R Malcom</creatorcontrib><creatorcontrib>Millard, Andrew C</creatorcontrib><creatorcontrib>Campagnola, Paul J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Optics letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Brown, Jr, R Malcom</au><au>Millard, Andrew C</au><au>Campagnola, Paul J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Macromolecular structure of cellulose studied by second-harmonic generation imaging microscopy</atitle><jtitle>Optics letters</jtitle><addtitle>Opt Lett</addtitle><date>2003-11-15</date><risdate>2003</risdate><volume>28</volume><issue>22</issue><spage>2207</spage><epage>2209</epage><pages>2207-2209</pages><issn>0146-9592</issn><eissn>1539-4794</eissn><abstract>The macromolecular structure of purified cellulose samples is studied by second-harmonic generation (SHG) imaging microscopy. 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| ispartof | Optics letters, 2003-11, Vol.28 (22), p.2207-2209 |
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| language | eng |
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| source | MEDLINE; Optica Publishing Group Journals |
| subjects | Acetobacter - chemistry Anisotropy Cellulose - chemistry Chlorophyta - chemistry Diagnostic Imaging Macromolecular Substances Microscopy, Confocal Microscopy, Polarization Molecular Structure Optics and Photonics |
| title | Macromolecular structure of cellulose studied by second-harmonic generation imaging microscopy |
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