Mechanism of Repression by Bacillus subtilis CcpC, a LysR Family Regulator
Bacillus subtilis CcpC is a LysR family transcriptional regulatory protein that negatively regulates genes encoding enzymes of the tricarboxylic acid branch of the Krebs cycle. In the present work, the promoter region of the aconitase ( citB) gene was used to investigate the mechanism of repression...
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| Veröffentlicht in: | Journal of molecular biology 2003-12, Vol.334 (4), p.609-624 |
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| creator | Kim, Sam-In Jourlin-Castelli, Cécile Wellington, Stephen R. Sonenshein, Abraham L. |
| description | Bacillus subtilis CcpC is a LysR family transcriptional regulatory protein that negatively regulates genes encoding enzymes of the tricarboxylic acid branch of the Krebs cycle. In the present work, the promoter region of the aconitase (
citB) gene was used to investigate the mechanism of repression by CcpC. The binding of CcpC to the
citB promoter region was shown to depend on DNA elements located near positions −66 and −27. Binding to these elements induced a bend in the DNA at position −41. Introduction of mutations in the −27 region and the presence of citrate, the inducer, had similar effects. In either case,
citB expression was derepressed
in vivo, the affinity of CcpC binding was reduced
in vitro, the angle of the bend was relaxed, and RNA polymerase gained greater access to the −35 region of the promoter. |
| doi_str_mv | 10.1016/j.jmb.2003.09.078 |
| format | Article |
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citB) gene was used to investigate the mechanism of repression by CcpC. The binding of CcpC to the
citB promoter region was shown to depend on DNA elements located near positions −66 and −27. Binding to these elements induced a bend in the DNA at position −41. Introduction of mutations in the −27 region and the presence of citrate, the inducer, had similar effects. In either case,
citB expression was derepressed
in vivo, the affinity of CcpC binding was reduced
in vitro, the angle of the bend was relaxed, and RNA polymerase gained greater access to the −35 region of the promoter.</description><identifier>ISSN: 0022-2836</identifier><identifier>EISSN: 1089-8638</identifier><identifier>DOI: 10.1016/j.jmb.2003.09.078</identifier><identifier>PMID: 14636591</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Aconitate Hydratase - genetics ; Aconitate Hydratase - metabolism ; Bacillus subtilis ; Bacillus subtilis - enzymology ; Bacillus subtilis - genetics ; Bacillus subtilis - metabolism ; Bacterial Proteins - metabolism ; Base Sequence ; catabolite repression ; CcpC ; CcpC protein ; citB ; CitB protein ; citrate ; Citric Acid - metabolism ; DNA Footprinting ; DNA-Binding Proteins - metabolism ; DNA-Directed RNA Polymerases - metabolism ; Gene Expression Regulation, Bacterial ; LysR protein ; Macromolecular Substances ; Molecular Sequence Data ; Nucleic Acid Conformation ; Promoter Regions, Genetic ; Protein Binding ; Recombinant Fusion Proteins - genetics ; Recombinant Fusion Proteins - metabolism ; Repressor Proteins - metabolism</subject><ispartof>Journal of molecular biology, 2003-12, Vol.334 (4), p.609-624</ispartof><rights>2003 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c380t-87ac31732b97f6882bf2ee0bcc827e2ba6881eea1f0aa3abbbe5b520162fb39b3</citedby><cites>FETCH-LOGICAL-c380t-87ac31732b97f6882bf2ee0bcc827e2ba6881eea1f0aa3abbbe5b520162fb39b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jmb.2003.09.078$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14636591$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Sam-In</creatorcontrib><creatorcontrib>Jourlin-Castelli, Cécile</creatorcontrib><creatorcontrib>Wellington, Stephen R.</creatorcontrib><creatorcontrib>Sonenshein, Abraham L.</creatorcontrib><title>Mechanism of Repression by Bacillus subtilis CcpC, a LysR Family Regulator</title><title>Journal of molecular biology</title><addtitle>J Mol Biol</addtitle><description>Bacillus subtilis CcpC is a LysR family transcriptional regulatory protein that negatively regulates genes encoding enzymes of the tricarboxylic acid branch of the Krebs cycle. In the present work, the promoter region of the aconitase (
citB) gene was used to investigate the mechanism of repression by CcpC. The binding of CcpC to the
citB promoter region was shown to depend on DNA elements located near positions −66 and −27. Binding to these elements induced a bend in the DNA at position −41. Introduction of mutations in the −27 region and the presence of citrate, the inducer, had similar effects. In either case,
citB expression was derepressed
in vivo, the affinity of CcpC binding was reduced
in vitro, the angle of the bend was relaxed, and RNA polymerase gained greater access to the −35 region of the promoter.</description><subject>Aconitate Hydratase - genetics</subject><subject>Aconitate Hydratase - metabolism</subject><subject>Bacillus subtilis</subject><subject>Bacillus subtilis - enzymology</subject><subject>Bacillus subtilis - genetics</subject><subject>Bacillus subtilis - metabolism</subject><subject>Bacterial Proteins - metabolism</subject><subject>Base Sequence</subject><subject>catabolite repression</subject><subject>CcpC</subject><subject>CcpC protein</subject><subject>citB</subject><subject>CitB protein</subject><subject>citrate</subject><subject>Citric Acid - metabolism</subject><subject>DNA Footprinting</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>DNA-Directed RNA Polymerases - metabolism</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>LysR protein</subject><subject>Macromolecular Substances</subject><subject>Molecular Sequence Data</subject><subject>Nucleic Acid Conformation</subject><subject>Promoter Regions, Genetic</subject><subject>Protein Binding</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>Recombinant Fusion Proteins - metabolism</subject><subject>Repressor Proteins - metabolism</subject><issn>0022-2836</issn><issn>1089-8638</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1LAzEURYMotlZ_gBvJypUzviSdmQyutFg_qAhF1yFJ32jKTKcmM0L_vSktuNPVg8u5F94h5JxByoDl18t02ZiUA4gUyhQKeUCGDGSZyFzIQzIE4DzhUuQDchLCEgAyMZbHZMDGucizkg3J8wvaT71yoaFtRee49hiCa1fUbOidtq6u-0BDbzpXu0Andj25oprONmFOp7px9SZ2Pvpad60_JUeVrgOe7e-IvE_v3yaPyez14WlyO0uskNAlstBWsEJwUxZVLiU3FUcEY63kBXKjY8YQNatAa6GNMZiZjMd_eWVEacSIXO5217796jF0qnHBYl3rFbZ9UAUbM1Yw_i_ISs4zASKCbAda34bgsVJr7xrtN4qB2ppWSxVNq61pBaWKpmPnYj_emwYXv4292gjc7ACMLr4dehWsw5XFhfNoO7Vo3R_zP8cAjf4</recordid><startdate>20031205</startdate><enddate>20031205</enddate><creator>Kim, Sam-In</creator><creator>Jourlin-Castelli, Cécile</creator><creator>Wellington, Stephen R.</creator><creator>Sonenshein, Abraham L.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20031205</creationdate><title>Mechanism of Repression by Bacillus subtilis CcpC, a LysR Family Regulator</title><author>Kim, Sam-In ; Jourlin-Castelli, Cécile ; Wellington, Stephen R. ; Sonenshein, Abraham L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c380t-87ac31732b97f6882bf2ee0bcc827e2ba6881eea1f0aa3abbbe5b520162fb39b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Aconitate Hydratase - genetics</topic><topic>Aconitate Hydratase - metabolism</topic><topic>Bacillus subtilis</topic><topic>Bacillus subtilis - enzymology</topic><topic>Bacillus subtilis - genetics</topic><topic>Bacillus subtilis - metabolism</topic><topic>Bacterial Proteins - metabolism</topic><topic>Base Sequence</topic><topic>catabolite repression</topic><topic>CcpC</topic><topic>CcpC protein</topic><topic>citB</topic><topic>CitB protein</topic><topic>citrate</topic><topic>Citric Acid - metabolism</topic><topic>DNA Footprinting</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>DNA-Directed RNA Polymerases - metabolism</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>LysR protein</topic><topic>Macromolecular Substances</topic><topic>Molecular Sequence Data</topic><topic>Nucleic Acid Conformation</topic><topic>Promoter Regions, Genetic</topic><topic>Protein Binding</topic><topic>Recombinant Fusion Proteins - genetics</topic><topic>Recombinant Fusion Proteins - metabolism</topic><topic>Repressor Proteins - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Sam-In</creatorcontrib><creatorcontrib>Jourlin-Castelli, Cécile</creatorcontrib><creatorcontrib>Wellington, Stephen R.</creatorcontrib><creatorcontrib>Sonenshein, Abraham L.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Sam-In</au><au>Jourlin-Castelli, Cécile</au><au>Wellington, Stephen R.</au><au>Sonenshein, Abraham L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mechanism of Repression by Bacillus subtilis CcpC, a LysR Family Regulator</atitle><jtitle>Journal of molecular biology</jtitle><addtitle>J Mol Biol</addtitle><date>2003-12-05</date><risdate>2003</risdate><volume>334</volume><issue>4</issue><spage>609</spage><epage>624</epage><pages>609-624</pages><issn>0022-2836</issn><eissn>1089-8638</eissn><abstract>Bacillus subtilis CcpC is a LysR family transcriptional regulatory protein that negatively regulates genes encoding enzymes of the tricarboxylic acid branch of the Krebs cycle. In the present work, the promoter region of the aconitase (
citB) gene was used to investigate the mechanism of repression by CcpC. The binding of CcpC to the
citB promoter region was shown to depend on DNA elements located near positions −66 and −27. Binding to these elements induced a bend in the DNA at position −41. Introduction of mutations in the −27 region and the presence of citrate, the inducer, had similar effects. In either case,
citB expression was derepressed
in vivo, the affinity of CcpC binding was reduced
in vitro, the angle of the bend was relaxed, and RNA polymerase gained greater access to the −35 region of the promoter.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>14636591</pmid><doi>10.1016/j.jmb.2003.09.078</doi><tpages>16</tpages></addata></record> |
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| ispartof | Journal of molecular biology, 2003-12, Vol.334 (4), p.609-624 |
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| subjects | Aconitate Hydratase - genetics Aconitate Hydratase - metabolism Bacillus subtilis Bacillus subtilis - enzymology Bacillus subtilis - genetics Bacillus subtilis - metabolism Bacterial Proteins - metabolism Base Sequence catabolite repression CcpC CcpC protein citB CitB protein citrate Citric Acid - metabolism DNA Footprinting DNA-Binding Proteins - metabolism DNA-Directed RNA Polymerases - metabolism Gene Expression Regulation, Bacterial LysR protein Macromolecular Substances Molecular Sequence Data Nucleic Acid Conformation Promoter Regions, Genetic Protein Binding Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism Repressor Proteins - metabolism |
| title | Mechanism of Repression by Bacillus subtilis CcpC, a LysR Family Regulator |
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