Molecular cloning and nucleotide sequence of a new P450 gene, CYP319A1, from the cattle tick, Boophilus microplus
We have isolated and sequenced a novel P450 gene ( CYP319A1) from the cattle tick, Boophilus microplus. The CYP319A1 cDNA encodes a protein of 531 amino acids with an estimated molecular weight of 60.9 k. It contains all highly conserved motifs characteristic of P450 enzymes. Comparison of deduced a...
Gespeichert in:
| Veröffentlicht in: | Insect biochemistry and molecular biology 2002-03, Vol.32 (3), p.303-309 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 309 |
|---|---|
| container_issue | 3 |
| container_start_page | 303 |
| container_title | Insect biochemistry and molecular biology |
| container_volume | 32 |
| creator | He, H Chen, A.C Davey, R.B Ivie, G.W |
| description | We have isolated and sequenced a novel P450 gene (
CYP319A1) from the cattle tick,
Boophilus microplus. The
CYP319A1 cDNA encodes a protein of 531 amino acids with an estimated molecular weight of 60.9
k. It contains all highly conserved motifs characteristic of P450 enzymes. Comparison of deduced amino acid sequence with other CYP members shows that the CYP319A1 is more closely related to CYP4 family, but its overall identity to the CYP4 family is less than 40%. Therefore, it was assigned to a new P450 family by the P450 nomenclature committee. A pseudogene which shares high homology with the
CYP319A1 was identified. Analysis of genomic sequence of the pseudogene indicated that the pseudogene contains two additional DNA inserts in the coding region, which disrupt the open reading frame. RT-PCR analysis showed that CYP319A1 is expressed in both susceptible and acaricide-resistant ticks. |
| doi_str_mv | 10.1016/S0965-1748(01)00091-1 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_71409041</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0965174801000911</els_id><sourcerecordid>71409041</sourcerecordid><originalsourceid>FETCH-LOGICAL-c392t-d2b2a3233e3db6080dde3db013aea3242e67101300ba722d6c212b7bff01b3913</originalsourceid><addsrcrecordid>eNqFkU9v1DAQxS0EokvhI4B8QiBtyoztzZ9TVVbQIhVRCThwshx70hqSeGsnVHx7nO4Kjj3NaPSbeZr3GHuJcIKA5buv0JSbAitVvwF8CwANFviIrbCumgKEgsds9Q85Ys9S-pkhpTbVU3aEWIOqQazY7efQk517E7ntw-jHa25Gx8fZ9hQm74gnup1ptMRDxw0f6Y5fqQ3waxppzbc_riQ2Z7jmXQwDn26IWzNNPfHJ219r_j6E3Y3v58QHb2PY5e45e9KZPtGLQz1m3z9--La9KC6_nH_anl0WVjZiKpxohZFCSpKuLaEG55YOUBrKcyWorLIREqA1lRCutAJFW7VdB9jKBuUxe72_u4shf5AmPfhkqe_NSGFOukIFDaiHQawVyBqbDG72YP4kpUid3kU_mPhHI-glFH0fil4c14D6PhS9CLw6CMztQO7_1iGFDJzuAcp-_PYUdbJ-8dz5SHbSLvgHJP4C4p2Z6g</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>18403819</pqid></control><display><type>article</type><title>Molecular cloning and nucleotide sequence of a new P450 gene, CYP319A1, from the cattle tick, Boophilus microplus</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>He, H ; Chen, A.C ; Davey, R.B ; Ivie, G.W</creator><creatorcontrib>He, H ; Chen, A.C ; Davey, R.B ; Ivie, G.W</creatorcontrib><description>We have isolated and sequenced a novel P450 gene (
CYP319A1) from the cattle tick,
Boophilus microplus. The
CYP319A1 cDNA encodes a protein of 531 amino acids with an estimated molecular weight of 60.9
k. It contains all highly conserved motifs characteristic of P450 enzymes. Comparison of deduced amino acid sequence with other CYP members shows that the CYP319A1 is more closely related to CYP4 family, but its overall identity to the CYP4 family is less than 40%. Therefore, it was assigned to a new P450 family by the P450 nomenclature committee. A pseudogene which shares high homology with the
CYP319A1 was identified. Analysis of genomic sequence of the pseudogene indicated that the pseudogene contains two additional DNA inserts in the coding region, which disrupt the open reading frame. RT-PCR analysis showed that CYP319A1 is expressed in both susceptible and acaricide-resistant ticks.</description><identifier>ISSN: 0965-1748</identifier><identifier>EISSN: 1879-0240</identifier><identifier>DOI: 10.1016/S0965-1748(01)00091-1</identifier><identifier>PMID: 11804802</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Amino Acid Sequence ; Animals ; Base Sequence ; cDNA ; Cloning, Molecular ; Cytochrome P-450 Enzyme System - classification ; Cytochrome P-450 Enzyme System - genetics ; Cytochrome P450 ; DNA, Complementary ; Humans ; Molecular Sequence Data ; Phylogeny ; Pseudogene ; Pseudogenes ; Sequence Analysis, DNA ; Sequence Homology, Amino Acid ; Ticks - enzymology ; Ticks - genetics</subject><ispartof>Insect biochemistry and molecular biology, 2002-03, Vol.32 (3), p.303-309</ispartof><rights>2002</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c392t-d2b2a3233e3db6080dde3db013aea3242e67101300ba722d6c212b7bff01b3913</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0965174801000911$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11804802$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>He, H</creatorcontrib><creatorcontrib>Chen, A.C</creatorcontrib><creatorcontrib>Davey, R.B</creatorcontrib><creatorcontrib>Ivie, G.W</creatorcontrib><title>Molecular cloning and nucleotide sequence of a new P450 gene, CYP319A1, from the cattle tick, Boophilus microplus</title><title>Insect biochemistry and molecular biology</title><addtitle>Insect Biochem Mol Biol</addtitle><description>We have isolated and sequenced a novel P450 gene (
CYP319A1) from the cattle tick,
Boophilus microplus. The
CYP319A1 cDNA encodes a protein of 531 amino acids with an estimated molecular weight of 60.9
k. It contains all highly conserved motifs characteristic of P450 enzymes. Comparison of deduced amino acid sequence with other CYP members shows that the CYP319A1 is more closely related to CYP4 family, but its overall identity to the CYP4 family is less than 40%. Therefore, it was assigned to a new P450 family by the P450 nomenclature committee. A pseudogene which shares high homology with the
CYP319A1 was identified. Analysis of genomic sequence of the pseudogene indicated that the pseudogene contains two additional DNA inserts in the coding region, which disrupt the open reading frame. RT-PCR analysis showed that CYP319A1 is expressed in both susceptible and acaricide-resistant ticks.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>cDNA</subject><subject>Cloning, Molecular</subject><subject>Cytochrome P-450 Enzyme System - classification</subject><subject>Cytochrome P-450 Enzyme System - genetics</subject><subject>Cytochrome P450</subject><subject>DNA, Complementary</subject><subject>Humans</subject><subject>Molecular Sequence Data</subject><subject>Phylogeny</subject><subject>Pseudogene</subject><subject>Pseudogenes</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Homology, Amino Acid</subject><subject>Ticks - enzymology</subject><subject>Ticks - genetics</subject><issn>0965-1748</issn><issn>1879-0240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9v1DAQxS0EokvhI4B8QiBtyoztzZ9TVVbQIhVRCThwshx70hqSeGsnVHx7nO4Kjj3NaPSbeZr3GHuJcIKA5buv0JSbAitVvwF8CwANFviIrbCumgKEgsds9Q85Ys9S-pkhpTbVU3aEWIOqQazY7efQk517E7ntw-jHa25Gx8fZ9hQm74gnup1ptMRDxw0f6Y5fqQ3waxppzbc_riQ2Z7jmXQwDn26IWzNNPfHJ219r_j6E3Y3v58QHb2PY5e45e9KZPtGLQz1m3z9--La9KC6_nH_anl0WVjZiKpxohZFCSpKuLaEG55YOUBrKcyWorLIREqA1lRCutAJFW7VdB9jKBuUxe72_u4shf5AmPfhkqe_NSGFOukIFDaiHQawVyBqbDG72YP4kpUid3kU_mPhHI-glFH0fil4c14D6PhS9CLw6CMztQO7_1iGFDJzuAcp-_PYUdbJ-8dz5SHbSLvgHJP4C4p2Z6g</recordid><startdate>20020301</startdate><enddate>20020301</enddate><creator>He, H</creator><creator>Chen, A.C</creator><creator>Davey, R.B</creator><creator>Ivie, G.W</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20020301</creationdate><title>Molecular cloning and nucleotide sequence of a new P450 gene, CYP319A1, from the cattle tick, Boophilus microplus</title><author>He, H ; Chen, A.C ; Davey, R.B ; Ivie, G.W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c392t-d2b2a3233e3db6080dde3db013aea3242e67101300ba722d6c212b7bff01b3913</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>cDNA</topic><topic>Cloning, Molecular</topic><topic>Cytochrome P-450 Enzyme System - classification</topic><topic>Cytochrome P-450 Enzyme System - genetics</topic><topic>Cytochrome P450</topic><topic>DNA, Complementary</topic><topic>Humans</topic><topic>Molecular Sequence Data</topic><topic>Phylogeny</topic><topic>Pseudogene</topic><topic>Pseudogenes</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Homology, Amino Acid</topic><topic>Ticks - enzymology</topic><topic>Ticks - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>He, H</creatorcontrib><creatorcontrib>Chen, A.C</creatorcontrib><creatorcontrib>Davey, R.B</creatorcontrib><creatorcontrib>Ivie, G.W</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Insect biochemistry and molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>He, H</au><au>Chen, A.C</au><au>Davey, R.B</au><au>Ivie, G.W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular cloning and nucleotide sequence of a new P450 gene, CYP319A1, from the cattle tick, Boophilus microplus</atitle><jtitle>Insect biochemistry and molecular biology</jtitle><addtitle>Insect Biochem Mol Biol</addtitle><date>2002-03-01</date><risdate>2002</risdate><volume>32</volume><issue>3</issue><spage>303</spage><epage>309</epage><pages>303-309</pages><issn>0965-1748</issn><eissn>1879-0240</eissn><abstract>We have isolated and sequenced a novel P450 gene (
CYP319A1) from the cattle tick,
Boophilus microplus. The
CYP319A1 cDNA encodes a protein of 531 amino acids with an estimated molecular weight of 60.9
k. It contains all highly conserved motifs characteristic of P450 enzymes. Comparison of deduced amino acid sequence with other CYP members shows that the CYP319A1 is more closely related to CYP4 family, but its overall identity to the CYP4 family is less than 40%. Therefore, it was assigned to a new P450 family by the P450 nomenclature committee. A pseudogene which shares high homology with the
CYP319A1 was identified. Analysis of genomic sequence of the pseudogene indicated that the pseudogene contains two additional DNA inserts in the coding region, which disrupt the open reading frame. RT-PCR analysis showed that CYP319A1 is expressed in both susceptible and acaricide-resistant ticks.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>11804802</pmid><doi>10.1016/S0965-1748(01)00091-1</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0965-1748 |
| ispartof | Insect biochemistry and molecular biology, 2002-03, Vol.32 (3), p.303-309 |
| issn | 0965-1748 1879-0240 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_71409041 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Amino Acid Sequence Animals Base Sequence cDNA Cloning, Molecular Cytochrome P-450 Enzyme System - classification Cytochrome P-450 Enzyme System - genetics Cytochrome P450 DNA, Complementary Humans Molecular Sequence Data Phylogeny Pseudogene Pseudogenes Sequence Analysis, DNA Sequence Homology, Amino Acid Ticks - enzymology Ticks - genetics |
| title | Molecular cloning and nucleotide sequence of a new P450 gene, CYP319A1, from the cattle tick, Boophilus microplus |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-13T22%3A44%3A49IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Molecular%20cloning%20and%20nucleotide%20sequence%20of%20a%20new%20P450%20gene,%20CYP319A1,%20from%20the%20cattle%20tick,%20Boophilus%20microplus&rft.jtitle=Insect%20biochemistry%20and%20molecular%20biology&rft.au=He,%20H&rft.date=2002-03-01&rft.volume=32&rft.issue=3&rft.spage=303&rft.epage=309&rft.pages=303-309&rft.issn=0965-1748&rft.eissn=1879-0240&rft_id=info:doi/10.1016/S0965-1748(01)00091-1&rft_dat=%3Cproquest_cross%3E71409041%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=18403819&rft_id=info:pmid/11804802&rft_els_id=S0965174801000911&rfr_iscdi=true |