Cryptosporidium in farmed animals: the detection of a novel isolate in sheep
We describe the discovery of polymorphisms in the Cryptosporidium oocyst wall protein (COWP) gene conferring a novel restriction fragment length polymorphism (RFLP) pattern in 26/60 (43%) isolates from a flock of sheep sampled following a waterborne outbreak of human cryptosporidiosis. The sheep iso...
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| Veröffentlicht in: | International journal for parasitology 2002, Vol.32 (1), p.21-26 |
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| creator | Chalmers, R.M Elwin, K Reilly, W.J Irvine, H Thomas, A.L Hunter, P.R |
| description | We describe the discovery of polymorphisms in the
Cryptosporidium oocyst wall protein (COWP) gene conferring a novel restriction fragment length polymorphism (RFLP) pattern in 26/60 (43%) isolates from a flock of sheep sampled following a waterborne outbreak of human cryptosporidiosis. The sheep isolates showed identical PCR-RFLP patterns to each other by COWP genotyping but different from those of most currently recognised genotypes, including the major
Cryptosporidium parvum genotypes 1 and 2. Sequence analysis of the 550
bp amplicon from the COWP gene was compared with a DNA coding region employed in previous studies and showed the novel isolate to differ from other
Cryptosporidium species and
C. parvum isolates by 7–21%. The sheep-derived isolates were compared at this and further three
Cryptosporidium gene loci with isolates from other farmed animals. The loci employed were one in the thrombospondin related adhesive protein (TRAP-C2) gene and two in the 70
kDa heat shock protein (HSP70) gene (CPHSP1 and 2). Other animal samples tested in our laboratory were from clinically ill animals and all contained
C. parvum genotype 2. The sheep in which the novel isolate was identified were healthy and showed no symptoms of cryptosporidiosis, and the novel sheep isolate could represent a non-pathogenic strain. Our studies suggest that a previously undetected
Cryptosporidium sub-type may exist in sheep populations, reflecting the increasingly recognised diversity within the parasite genus. |
| doi_str_mv | 10.1016/S0020-7519(01)00309-5 |
| format | Article |
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Cryptosporidium oocyst wall protein (COWP) gene conferring a novel restriction fragment length polymorphism (RFLP) pattern in 26/60 (43%) isolates from a flock of sheep sampled following a waterborne outbreak of human cryptosporidiosis. The sheep isolates showed identical PCR-RFLP patterns to each other by COWP genotyping but different from those of most currently recognised genotypes, including the major
Cryptosporidium parvum genotypes 1 and 2. Sequence analysis of the 550
bp amplicon from the COWP gene was compared with a DNA coding region employed in previous studies and showed the novel isolate to differ from other
Cryptosporidium species and
C. parvum isolates by 7–21%. The sheep-derived isolates were compared at this and further three
Cryptosporidium gene loci with isolates from other farmed animals. The loci employed were one in the thrombospondin related adhesive protein (TRAP-C2) gene and two in the 70
kDa heat shock protein (HSP70) gene (CPHSP1 and 2). Other animal samples tested in our laboratory were from clinically ill animals and all contained
C. parvum genotype 2. The sheep in which the novel isolate was identified were healthy and showed no symptoms of cryptosporidiosis, and the novel sheep isolate could represent a non-pathogenic strain. Our studies suggest that a previously undetected
Cryptosporidium sub-type may exist in sheep populations, reflecting the increasingly recognised diversity within the parasite genus.</description><identifier>ISSN: 0020-7519</identifier><identifier>EISSN: 1879-0135</identifier><identifier>DOI: 10.1016/S0020-7519(01)00309-5</identifier><identifier>PMID: 11796119</identifier><identifier>CODEN: IJPYBT</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Animal productions ; Animals ; Biological and medical sciences ; Cryptosporidiosis - epidemiology ; Cryptosporidiosis - parasitology ; Cryptosporidiosis - veterinary ; Cryptosporidium ; Cryptosporidium - classification ; Cryptosporidium - genetics ; Cryptosporidium - isolation & purification ; Disease Outbreaks ; DNA, Protozoan - chemistry ; DNA, Protozoan - genetics ; Feces - parasitology ; Fluorescent Antibody Technique, Indirect - veterinary ; Fundamental and applied biological sciences. Psychology ; Genetics of eukaryotes. Biological and molecular evolution ; Genotyping ; HSP70 Heat-Shock Proteins - chemistry ; HSP70 Heat-Shock Proteins - genetics ; Humans ; Polymerase Chain Reaction - veterinary ; Polymorphism, Restriction Fragment Length ; Population genetics, reproduction patterns ; Prevalence ; Protozoa ; Protozoan Proteins - chemistry ; Protozoan Proteins - classification ; Protozoan Proteins - genetics ; Sequence Analysis, DNA ; Sequencing ; Sheep ; Sheep Diseases - epidemiology ; Sheep Diseases - parasitology ; Terrestrial animal productions ; Vertebrates</subject><ispartof>International journal for parasitology, 2002, Vol.32 (1), p.21-26</ispartof><rights>2002 Australian Society for Parasitology Inc</rights><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c422t-617f6fca1ba5036bfc2c1c28330036a5e7723a407494ae790929118da3c88bfa3</citedby><cites>FETCH-LOGICAL-c422t-617f6fca1ba5036bfc2c1c28330036a5e7723a407494ae790929118da3c88bfa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0020751901003095$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,4010,27900,27901,27902,65534</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13454210$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11796119$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chalmers, R.M</creatorcontrib><creatorcontrib>Elwin, K</creatorcontrib><creatorcontrib>Reilly, W.J</creatorcontrib><creatorcontrib>Irvine, H</creatorcontrib><creatorcontrib>Thomas, A.L</creatorcontrib><creatorcontrib>Hunter, P.R</creatorcontrib><title>Cryptosporidium in farmed animals: the detection of a novel isolate in sheep</title><title>International journal for parasitology</title><addtitle>Int J Parasitol</addtitle><description>We describe the discovery of polymorphisms in the
Cryptosporidium oocyst wall protein (COWP) gene conferring a novel restriction fragment length polymorphism (RFLP) pattern in 26/60 (43%) isolates from a flock of sheep sampled following a waterborne outbreak of human cryptosporidiosis. The sheep isolates showed identical PCR-RFLP patterns to each other by COWP genotyping but different from those of most currently recognised genotypes, including the major
Cryptosporidium parvum genotypes 1 and 2. Sequence analysis of the 550
bp amplicon from the COWP gene was compared with a DNA coding region employed in previous studies and showed the novel isolate to differ from other
Cryptosporidium species and
C. parvum isolates by 7–21%. The sheep-derived isolates were compared at this and further three
Cryptosporidium gene loci with isolates from other farmed animals. The loci employed were one in the thrombospondin related adhesive protein (TRAP-C2) gene and two in the 70
kDa heat shock protein (HSP70) gene (CPHSP1 and 2). Other animal samples tested in our laboratory were from clinically ill animals and all contained
C. parvum genotype 2. The sheep in which the novel isolate was identified were healthy and showed no symptoms of cryptosporidiosis, and the novel sheep isolate could represent a non-pathogenic strain. Our studies suggest that a previously undetected
Cryptosporidium sub-type may exist in sheep populations, reflecting the increasingly recognised diversity within the parasite genus.</description><subject>Animal productions</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cryptosporidiosis - epidemiology</subject><subject>Cryptosporidiosis - parasitology</subject><subject>Cryptosporidiosis - veterinary</subject><subject>Cryptosporidium</subject><subject>Cryptosporidium - classification</subject><subject>Cryptosporidium - genetics</subject><subject>Cryptosporidium - isolation & purification</subject><subject>Disease Outbreaks</subject><subject>DNA, Protozoan - chemistry</subject><subject>DNA, Protozoan - genetics</subject><subject>Feces - parasitology</subject><subject>Fluorescent Antibody Technique, Indirect - veterinary</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetics of eukaryotes. Biological and molecular evolution</subject><subject>Genotyping</subject><subject>HSP70 Heat-Shock Proteins - chemistry</subject><subject>HSP70 Heat-Shock Proteins - genetics</subject><subject>Humans</subject><subject>Polymerase Chain Reaction - veterinary</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>Population genetics, reproduction patterns</subject><subject>Prevalence</subject><subject>Protozoa</subject><subject>Protozoan Proteins - chemistry</subject><subject>Protozoan Proteins - classification</subject><subject>Protozoan Proteins - genetics</subject><subject>Sequence Analysis, DNA</subject><subject>Sequencing</subject><subject>Sheep</subject><subject>Sheep Diseases - epidemiology</subject><subject>Sheep Diseases - parasitology</subject><subject>Terrestrial animal productions</subject><subject>Vertebrates</subject><issn>0020-7519</issn><issn>1879-0135</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1v1DAQhi0EokvhJ4ByoYJDykz8FfdSoRVf0kocgLPldcaqUTYOdrZS_3293RU99jSX552Z92HsLcIlAqpPvwA6aLVE8wHwIwAH08pnbIW9Ni0gl8_Z6j9yxl6V8hcAJRfiJTtD1EYhmhXbrPPdvKQypxyHuN81cWqCyzsaGjfFnRvLVbPcUDPQQn6JaWpSaFwzpVsam1jS6BY6ZMoN0fyavQg1QW9O85z9-frl9_p7u_n57cf686b1ouuWVqEOKniHWyeBq23wnUff9ZzXGspJ0rrjToAWRjjSBkxnEPvBcd_32-D4Obs47p1z-rensthdLJ7G0U2U9sVqFNAL0z8JYs-VNEpVUB5Bn1MpmYKdc22f7yyCPfi2D77tQaYFtA--ray5d6cD-2119pg6Ca7A-xPgindjyG7ysTxyXEjRIVTu-shR9XYbKdviI02ehpireDuk-MQr91-SmyA</recordid><startdate>2002</startdate><enddate>2002</enddate><creator>Chalmers, R.M</creator><creator>Elwin, K</creator><creator>Reilly, W.J</creator><creator>Irvine, H</creator><creator>Thomas, A.L</creator><creator>Hunter, P.R</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>2002</creationdate><title>Cryptosporidium in farmed animals: the detection of a novel isolate in sheep</title><author>Chalmers, R.M ; Elwin, K ; Reilly, W.J ; Irvine, H ; Thomas, A.L ; Hunter, P.R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-617f6fca1ba5036bfc2c1c28330036a5e7723a407494ae790929118da3c88bfa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animal productions</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cryptosporidiosis - epidemiology</topic><topic>Cryptosporidiosis - parasitology</topic><topic>Cryptosporidiosis - veterinary</topic><topic>Cryptosporidium</topic><topic>Cryptosporidium - classification</topic><topic>Cryptosporidium - genetics</topic><topic>Cryptosporidium - isolation & purification</topic><topic>Disease Outbreaks</topic><topic>DNA, Protozoan - chemistry</topic><topic>DNA, Protozoan - genetics</topic><topic>Feces - parasitology</topic><topic>Fluorescent Antibody Technique, Indirect - veterinary</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetics of eukaryotes. Biological and molecular evolution</topic><topic>Genotyping</topic><topic>HSP70 Heat-Shock Proteins - chemistry</topic><topic>HSP70 Heat-Shock Proteins - genetics</topic><topic>Humans</topic><topic>Polymerase Chain Reaction - veterinary</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>Population genetics, reproduction patterns</topic><topic>Prevalence</topic><topic>Protozoa</topic><topic>Protozoan Proteins - chemistry</topic><topic>Protozoan Proteins - classification</topic><topic>Protozoan Proteins - genetics</topic><topic>Sequence Analysis, DNA</topic><topic>Sequencing</topic><topic>Sheep</topic><topic>Sheep Diseases - epidemiology</topic><topic>Sheep Diseases - parasitology</topic><topic>Terrestrial animal productions</topic><topic>Vertebrates</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chalmers, R.M</creatorcontrib><creatorcontrib>Elwin, K</creatorcontrib><creatorcontrib>Reilly, W.J</creatorcontrib><creatorcontrib>Irvine, H</creatorcontrib><creatorcontrib>Thomas, A.L</creatorcontrib><creatorcontrib>Hunter, P.R</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>International journal for parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chalmers, R.M</au><au>Elwin, K</au><au>Reilly, W.J</au><au>Irvine, H</au><au>Thomas, A.L</au><au>Hunter, P.R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cryptosporidium in farmed animals: the detection of a novel isolate in sheep</atitle><jtitle>International journal for parasitology</jtitle><addtitle>Int J Parasitol</addtitle><date>2002</date><risdate>2002</risdate><volume>32</volume><issue>1</issue><spage>21</spage><epage>26</epage><pages>21-26</pages><issn>0020-7519</issn><eissn>1879-0135</eissn><coden>IJPYBT</coden><abstract>We describe the discovery of polymorphisms in the
Cryptosporidium oocyst wall protein (COWP) gene conferring a novel restriction fragment length polymorphism (RFLP) pattern in 26/60 (43%) isolates from a flock of sheep sampled following a waterborne outbreak of human cryptosporidiosis. The sheep isolates showed identical PCR-RFLP patterns to each other by COWP genotyping but different from those of most currently recognised genotypes, including the major
Cryptosporidium parvum genotypes 1 and 2. Sequence analysis of the 550
bp amplicon from the COWP gene was compared with a DNA coding region employed in previous studies and showed the novel isolate to differ from other
Cryptosporidium species and
C. parvum isolates by 7–21%. The sheep-derived isolates were compared at this and further three
Cryptosporidium gene loci with isolates from other farmed animals. The loci employed were one in the thrombospondin related adhesive protein (TRAP-C2) gene and two in the 70
kDa heat shock protein (HSP70) gene (CPHSP1 and 2). Other animal samples tested in our laboratory were from clinically ill animals and all contained
C. parvum genotype 2. The sheep in which the novel isolate was identified were healthy and showed no symptoms of cryptosporidiosis, and the novel sheep isolate could represent a non-pathogenic strain. Our studies suggest that a previously undetected
Cryptosporidium sub-type may exist in sheep populations, reflecting the increasingly recognised diversity within the parasite genus.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>11796119</pmid><doi>10.1016/S0020-7519(01)00309-5</doi><tpages>6</tpages></addata></record> |
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| subjects | Animal productions Animals Biological and medical sciences Cryptosporidiosis - epidemiology Cryptosporidiosis - parasitology Cryptosporidiosis - veterinary Cryptosporidium Cryptosporidium - classification Cryptosporidium - genetics Cryptosporidium - isolation & purification Disease Outbreaks DNA, Protozoan - chemistry DNA, Protozoan - genetics Feces - parasitology Fluorescent Antibody Technique, Indirect - veterinary Fundamental and applied biological sciences. Psychology Genetics of eukaryotes. Biological and molecular evolution Genotyping HSP70 Heat-Shock Proteins - chemistry HSP70 Heat-Shock Proteins - genetics Humans Polymerase Chain Reaction - veterinary Polymorphism, Restriction Fragment Length Population genetics, reproduction patterns Prevalence Protozoa Protozoan Proteins - chemistry Protozoan Proteins - classification Protozoan Proteins - genetics Sequence Analysis, DNA Sequencing Sheep Sheep Diseases - epidemiology Sheep Diseases - parasitology Terrestrial animal productions Vertebrates |
| title | Cryptosporidium in farmed animals: the detection of a novel isolate in sheep |
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