Inability of a Variant Strain of Anaplasma phagocytophilum to Infect Mice
Nymphal Ixodes scapularis ticks were collected from several sites in Rhode Island. Polymerase chain reaction and DNA sequencing were used to determine the presence and prevalence of Anaplasma phagocytophilum human agent (AP-ha) and a genetic variant not associated with human disease (AP-variant 1)....
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| Veröffentlicht in: | The Journal of infectious diseases 2003-12, Vol.188 (11), p.1757-1763 |
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| container_issue | 11 |
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| container_title | The Journal of infectious diseases |
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| creator | Massung, Robert F. Priestley, Rachael A. Miller, Nathan J. Mather, Thomas N. Levin, Michael L. |
| description | Nymphal Ixodes scapularis ticks were collected from several sites in Rhode Island. Polymerase chain reaction and DNA sequencing were used to determine the presence and prevalence of Anaplasma phagocytophilum human agent (AP-ha) and a genetic variant not associated with human disease (AP-variant 1). The remaining ticks from each cohort were allowed to feed to repletion on either white-footed (Peromyscus leucopus) or DBA/2 (Mus musculus) mice. The engorged ticks and murine blood samples were evaluated for the presence of AP-ha and AP-variant 1. Although a high percentage of the infecting ticks harbored AP-variant 1, only AP-ha was amplified from the murine blood samples. Additional ticks were fed on immunocompromised SCID mice, and, again, only AP-ha was capable of establishing an infection, and only AP-ha could be detected by xenodiagnosis. These data suggest that AP-variant 1 cannot establish an infection in mice, and we propose that AP-variant 1 has an alternative natural reservoir, possibly white-tailed deer |
| doi_str_mv | 10.1086/379725 |
| format | Article |
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Polymerase chain reaction and DNA sequencing were used to determine the presence and prevalence of Anaplasma phagocytophilum human agent (AP-ha) and a genetic variant not associated with human disease (AP-variant 1). The remaining ticks from each cohort were allowed to feed to repletion on either white-footed (Peromyscus leucopus) or DBA/2 (Mus musculus) mice. The engorged ticks and murine blood samples were evaluated for the presence of AP-ha and AP-variant 1. Although a high percentage of the infecting ticks harbored AP-variant 1, only AP-ha was amplified from the murine blood samples. Additional ticks were fed on immunocompromised SCID mice, and, again, only AP-ha was capable of establishing an infection, and only AP-ha could be detected by xenodiagnosis. These data suggest that AP-variant 1 cannot establish an infection in mice, and we propose that AP-variant 1 has an alternative natural reservoir, possibly white-tailed deer</description><identifier>ISSN: 0022-1899</identifier><identifier>EISSN: 1537-6613</identifier><identifier>DOI: 10.1086/379725</identifier><identifier>PMID: 14639548</identifier><identifier>CODEN: JIDIAQ</identifier><language>eng</language><publisher>Chicago, IL: The University of Chicago Press</publisher><subject>Anaplasma phagocytophilum ; Anaplasma phagocytophilum - pathogenicity ; Animals ; Bacteremia - microbiology ; Bacteria ; Biological and medical sciences ; Deer ; Disease Reservoirs ; DNA ; Fundamental and applied biological sciences. Psychology ; Humans ; Infections ; Infectious diseases ; Ixodes - microbiology ; Ixodes scapularis ; Larvae ; Medical sciences ; Mice ; Mice, Inbred DBA ; Mice, SCID ; Microbiology ; Nymphs ; Peromyscus - microbiology ; Peromyscus leucopus ; Polymerase Chain Reaction ; Sequencing ; Ticks</subject><ispartof>The Journal of infectious diseases, 2003-12, Vol.188 (11), p.1757-1763</ispartof><rights>Copyright 2003 Infectious Diseases Society of America</rights><rights>2003 by the Infectious Diseases Society of America 2003</rights><rights>2004 INIST-CNRS</rights><rights>Copyright University of Chicago, acting through its Press Dec 1 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c449t-8682e61f043b7eaabff069037d29d05ed004bcdde4bfe1bfe6c748978501a173</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/30076765$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/30076765$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15359323$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14639548$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Massung, Robert F.</creatorcontrib><creatorcontrib>Priestley, Rachael A.</creatorcontrib><creatorcontrib>Miller, Nathan J.</creatorcontrib><creatorcontrib>Mather, Thomas N.</creatorcontrib><creatorcontrib>Levin, Michael L.</creatorcontrib><title>Inability of a Variant Strain of Anaplasma phagocytophilum to Infect Mice</title><title>The Journal of infectious diseases</title><addtitle>The Journal of Infectious Diseases</addtitle><addtitle>The Journal of Infectious Diseases</addtitle><description>Nymphal Ixodes scapularis ticks were collected from several sites in Rhode Island. Polymerase chain reaction and DNA sequencing were used to determine the presence and prevalence of Anaplasma phagocytophilum human agent (AP-ha) and a genetic variant not associated with human disease (AP-variant 1). The remaining ticks from each cohort were allowed to feed to repletion on either white-footed (Peromyscus leucopus) or DBA/2 (Mus musculus) mice. The engorged ticks and murine blood samples were evaluated for the presence of AP-ha and AP-variant 1. Although a high percentage of the infecting ticks harbored AP-variant 1, only AP-ha was amplified from the murine blood samples. Additional ticks were fed on immunocompromised SCID mice, and, again, only AP-ha was capable of establishing an infection, and only AP-ha could be detected by xenodiagnosis. These data suggest that AP-variant 1 cannot establish an infection in mice, and we propose that AP-variant 1 has an alternative natural reservoir, possibly white-tailed deer</description><subject>Anaplasma phagocytophilum</subject><subject>Anaplasma phagocytophilum - pathogenicity</subject><subject>Animals</subject><subject>Bacteremia - microbiology</subject><subject>Bacteria</subject><subject>Biological and medical sciences</subject><subject>Deer</subject><subject>Disease Reservoirs</subject><subject>DNA</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Infections</subject><subject>Infectious diseases</subject><subject>Ixodes - microbiology</subject><subject>Ixodes scapularis</subject><subject>Larvae</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred DBA</subject><subject>Mice, SCID</subject><subject>Microbiology</subject><subject>Nymphs</subject><subject>Peromyscus - microbiology</subject><subject>Peromyscus leucopus</subject><subject>Polymerase Chain Reaction</subject><subject>Sequencing</subject><subject>Ticks</subject><issn>0022-1899</issn><issn>1537-6613</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkdGL1DAQxoMo3rrqf6BUQd-qkyZNmsfz8O4WV3zwUPElTNPUy9o2NUnB_e_t0uUWBPEhDOT78c03M4Q8pfCGQiXeMqlkUd4jK1oymQtB2X2yAiiKnFZKnZFHMe4AgDMhH5IzygVTJa9WZLMZsHadS_vMtxlmXzA4HFL2OQV0w-HvfMCxw9hjNt7iD2_2yY-3rpv6LPlsM7TWpOyjM_YxedBiF-2TY12Tm8v3NxfX-fbT1ebifJsbzlXKK1EVVtB2jlJLi1i3LQgFTDaFaqC0zRyyNk1jed1aOj9hJK-UrEqgSCVbk9eL7Rj8r8nGpHsXje06HKyfopaUA4eK_RekqqCczutak5d_gTs_hWGeQRcFUxSYqE5uJvgYg231GFyPYa8p6MMF9HKBGXx-dJvq3jYn7LjyGXh1BDAa7NqAg3HxxJWsVKw45H-xcH4a_93s2cLsYvLhjmIAUkhx0PNFdzHZ33c6hp9aSCZLff3tu_66fQfw4UrqS_YHIzWtzQ</recordid><startdate>20031201</startdate><enddate>20031201</enddate><creator>Massung, Robert F.</creator><creator>Priestley, Rachael A.</creator><creator>Miller, Nathan J.</creator><creator>Mather, Thomas N.</creator><creator>Levin, Michael L.</creator><general>The University of Chicago Press</general><general>University of Chicago Press</general><general>Oxford University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20031201</creationdate><title>Inability of a Variant Strain of Anaplasma phagocytophilum to Infect Mice</title><author>Massung, Robert F. ; Priestley, Rachael A. ; Miller, Nathan J. ; Mather, Thomas N. ; Levin, Michael L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c449t-8682e61f043b7eaabff069037d29d05ed004bcdde4bfe1bfe6c748978501a173</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Anaplasma phagocytophilum</topic><topic>Anaplasma phagocytophilum - pathogenicity</topic><topic>Animals</topic><topic>Bacteremia - microbiology</topic><topic>Bacteria</topic><topic>Biological and medical sciences</topic><topic>Deer</topic><topic>Disease Reservoirs</topic><topic>DNA</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Infections</topic><topic>Infectious diseases</topic><topic>Ixodes - microbiology</topic><topic>Ixodes scapularis</topic><topic>Larvae</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred DBA</topic><topic>Mice, SCID</topic><topic>Microbiology</topic><topic>Nymphs</topic><topic>Peromyscus - microbiology</topic><topic>Peromyscus leucopus</topic><topic>Polymerase Chain Reaction</topic><topic>Sequencing</topic><topic>Ticks</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Massung, Robert F.</creatorcontrib><creatorcontrib>Priestley, Rachael A.</creatorcontrib><creatorcontrib>Miller, Nathan J.</creatorcontrib><creatorcontrib>Mather, Thomas N.</creatorcontrib><creatorcontrib>Levin, Michael L.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of infectious diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Massung, Robert F.</au><au>Priestley, Rachael A.</au><au>Miller, Nathan J.</au><au>Mather, Thomas N.</au><au>Levin, Michael L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inability of a Variant Strain of Anaplasma phagocytophilum to Infect Mice</atitle><jtitle>The Journal of infectious diseases</jtitle><stitle>The Journal of Infectious Diseases</stitle><addtitle>The Journal of Infectious Diseases</addtitle><date>2003-12-01</date><risdate>2003</risdate><volume>188</volume><issue>11</issue><spage>1757</spage><epage>1763</epage><pages>1757-1763</pages><issn>0022-1899</issn><eissn>1537-6613</eissn><coden>JIDIAQ</coden><abstract>Nymphal Ixodes scapularis ticks were collected from several sites in Rhode Island. Polymerase chain reaction and DNA sequencing were used to determine the presence and prevalence of Anaplasma phagocytophilum human agent (AP-ha) and a genetic variant not associated with human disease (AP-variant 1). The remaining ticks from each cohort were allowed to feed to repletion on either white-footed (Peromyscus leucopus) or DBA/2 (Mus musculus) mice. The engorged ticks and murine blood samples were evaluated for the presence of AP-ha and AP-variant 1. Although a high percentage of the infecting ticks harbored AP-variant 1, only AP-ha was amplified from the murine blood samples. Additional ticks were fed on immunocompromised SCID mice, and, again, only AP-ha was capable of establishing an infection, and only AP-ha could be detected by xenodiagnosis. These data suggest that AP-variant 1 cannot establish an infection in mice, and we propose that AP-variant 1 has an alternative natural reservoir, possibly white-tailed deer</abstract><cop>Chicago, IL</cop><pub>The University of Chicago Press</pub><pmid>14639548</pmid><doi>10.1086/379725</doi><tpages>7</tpages></addata></record> |
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| ispartof | The Journal of infectious diseases, 2003-12, Vol.188 (11), p.1757-1763 |
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| source | Jstor Complete Legacy; Oxford University Press Journals All Titles (1996-Current); MEDLINE; Alma/SFX Local Collection |
| subjects | Anaplasma phagocytophilum Anaplasma phagocytophilum - pathogenicity Animals Bacteremia - microbiology Bacteria Biological and medical sciences Deer Disease Reservoirs DNA Fundamental and applied biological sciences. Psychology Humans Infections Infectious diseases Ixodes - microbiology Ixodes scapularis Larvae Medical sciences Mice Mice, Inbred DBA Mice, SCID Microbiology Nymphs Peromyscus - microbiology Peromyscus leucopus Polymerase Chain Reaction Sequencing Ticks |
| title | Inability of a Variant Strain of Anaplasma phagocytophilum to Infect Mice |
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