The role of the Aspergillus niger furin-type protease gene in processing of fungal proproteins and fusion proteins : Evidence for alternative processing of recombinant (fusion-) proteins
We have characterized growth and protein processing characteristics of Aspergillus niger strains carrying a disrupted allele of the previously cloned and characterized kexB gene [Appl. Environ. Microbiol. 66 (2000) 363] encoding a furin-type endoprotease. Deletion of the single-copy gene confirms it...
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| Veröffentlicht in: | Journal of biotechnology 2003-12, Vol.106 (1), p.23-32 |
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| creator | Punt, P.J Drint-Kuijvenhoven, A Lokman, B.C Spencer, J.A Jeenes, D Archer, D.A van den Hondel, C.A.M.J.J |
| description | We have characterized growth and protein processing characteristics of
Aspergillus niger strains carrying a disrupted allele of the previously cloned and characterized
kexB gene [Appl. Environ. Microbiol. 66 (2000) 363] encoding a furin-type endoprotease. Deletion of the single-copy gene confirms it to be non-essential but disruptant strains exhibit a morphologically distinct phenotype characterized by hyperbranching. Processing of homologous pro-proteins and fusion proteins comprised of a heterologous protein fused down-stream of glucoamylase and separated at the fusion junction by an endoproteolytic cleavage site was compared in wildtype and mutant strains of
A. niger. We show that maturation of the native glucoamylase requires KexB, whereas maturation of aspergillopepsin does not. The processing of fusion proteins carrying Lys–Arg requires KexB, although alternative endoproteases are capable of cleaving protein fusions at sites adjacent to Lys–Arg. |
| doi_str_mv | 10.1016/j.jbiotec.2003.09.005 |
| format | Article |
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Aspergillus niger strains carrying a disrupted allele of the previously cloned and characterized
kexB gene [Appl. Environ. Microbiol. 66 (2000) 363] encoding a furin-type endoprotease. Deletion of the single-copy gene confirms it to be non-essential but disruptant strains exhibit a morphologically distinct phenotype characterized by hyperbranching. Processing of homologous pro-proteins and fusion proteins comprised of a heterologous protein fused down-stream of glucoamylase and separated at the fusion junction by an endoproteolytic cleavage site was compared in wildtype and mutant strains of
A. niger. We show that maturation of the native glucoamylase requires KexB, whereas maturation of aspergillopepsin does not. The processing of fusion proteins carrying Lys–Arg requires KexB, although alternative endoproteases are capable of cleaving protein fusions at sites adjacent to Lys–Arg.</description><identifier>ISSN: 0168-1656</identifier><identifier>EISSN: 1873-4863</identifier><identifier>DOI: 10.1016/j.jbiotec.2003.09.005</identifier><identifier>PMID: 14636707</identifier><identifier>CODEN: JBITD4</identifier><language>eng</language><publisher>Lausanne: Elsevier B.V</publisher><subject>aspergillopepsin ; Aspergillus niger ; Aspergillus niger - genetics ; Aspergillus niger - metabolism ; Biological and medical sciences ; Biotechnology ; Endopeptidases - genetics ; Endopeptidases - metabolism ; endoproteinase ; Filamentous fungi ; Fundamental and applied biological sciences. Psychology ; Fungal Proteins - biosynthesis ; Fungal Proteins - genetics ; furin ; Furin - genetics ; Furin - metabolism ; Fusion-proteins ; Gene Expression Regulation, Enzymologic - physiology ; Gene Expression Regulation, Fungal - physiology ; kexB gene ; Protease ; Protein processing ; Protein Processing, Post-Translational - physiology ; Protein secretion ; Recombinant Fusion Proteins - biosynthesis ; Recombinant Fusion Proteins - genetics ; Secretion carrier ; Transcriptional Activation - physiology</subject><ispartof>Journal of biotechnology, 2003-12, Vol.106 (1), p.23-32</ispartof><rights>2003 Elsevier B.V.</rights><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jbiotec.2003.09.005$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15318031$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14636707$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Punt, P.J</creatorcontrib><creatorcontrib>Drint-Kuijvenhoven, A</creatorcontrib><creatorcontrib>Lokman, B.C</creatorcontrib><creatorcontrib>Spencer, J.A</creatorcontrib><creatorcontrib>Jeenes, D</creatorcontrib><creatorcontrib>Archer, D.A</creatorcontrib><creatorcontrib>van den Hondel, C.A.M.J.J</creatorcontrib><title>The role of the Aspergillus niger furin-type protease gene in processing of fungal proproteins and fusion proteins : Evidence for alternative processing of recombinant (fusion-) proteins</title><title>Journal of biotechnology</title><addtitle>J Biotechnol</addtitle><description>We have characterized growth and protein processing characteristics of
Aspergillus niger strains carrying a disrupted allele of the previously cloned and characterized
kexB gene [Appl. Environ. Microbiol. 66 (2000) 363] encoding a furin-type endoprotease. Deletion of the single-copy gene confirms it to be non-essential but disruptant strains exhibit a morphologically distinct phenotype characterized by hyperbranching. Processing of homologous pro-proteins and fusion proteins comprised of a heterologous protein fused down-stream of glucoamylase and separated at the fusion junction by an endoproteolytic cleavage site was compared in wildtype and mutant strains of
A. niger. We show that maturation of the native glucoamylase requires KexB, whereas maturation of aspergillopepsin does not. The processing of fusion proteins carrying Lys–Arg requires KexB, although alternative endoproteases are capable of cleaving protein fusions at sites adjacent to Lys–Arg.</description><subject>aspergillopepsin</subject><subject>Aspergillus niger</subject><subject>Aspergillus niger - genetics</subject><subject>Aspergillus niger - metabolism</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Endopeptidases - genetics</subject><subject>Endopeptidases - metabolism</subject><subject>endoproteinase</subject><subject>Filamentous fungi</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungal Proteins - biosynthesis</subject><subject>Fungal Proteins - genetics</subject><subject>furin</subject><subject>Furin - genetics</subject><subject>Furin - metabolism</subject><subject>Fusion-proteins</subject><subject>Gene Expression Regulation, Enzymologic - physiology</subject><subject>Gene Expression Regulation, Fungal - physiology</subject><subject>kexB gene</subject><subject>Protease</subject><subject>Protein processing</subject><subject>Protein Processing, Post-Translational - physiology</subject><subject>Protein secretion</subject><subject>Recombinant Fusion Proteins - biosynthesis</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>Secretion carrier</subject><subject>Transcriptional Activation - physiology</subject><issn>0168-1656</issn><issn>1873-4863</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFksFu1DAQhi0EotvCI4B8AcEhYRwnjsMFVVULSJW4lLPl2OPgVdZZ7GSlvhpPh9NdisSFk61f3_yjmfkJecWgZMDEh2257f00oykrAF5CVwI0T8iGyZYXtRT8KdlkThZMNOKMnKe0BYC6a9hzcsZqwUUL7Yb8uvuBNE4j0snROf8v0x7j4MdxSTT4ASN1S_ShmO_3SPcxd9QJ6YABqQ-rYDAlH4a13i1h0OMqPoA-JKqDzXLyU6CP2kd6ffAWg0Hqpkj1OGMMevYH_Mcvopl2vQ86zPTd0aV4_-jzgjxzekz48vRekO8313dXX4rbb5-_Xl3eFsirei5co0UvteykZI6xhmnuBOdSyobxqu1FB9a2PXRaV2CMsMJpUSETtsUOas4vyNujb278c8E0q51PBsdRB5yWpFpWAwdo_wuyrqpAcJbB1ydw6Xdo1T76nY736s9ZMvDmBOhk9OiiDsanv1zDmYQHo09HDvP8B49RJePXxVqfdzcrO3nFQK2BUVt1CoxaA6OgUzkw_DeeEbe0</recordid><startdate>20031205</startdate><enddate>20031205</enddate><creator>Punt, P.J</creator><creator>Drint-Kuijvenhoven, A</creator><creator>Lokman, B.C</creator><creator>Spencer, J.A</creator><creator>Jeenes, D</creator><creator>Archer, D.A</creator><creator>van den Hondel, C.A.M.J.J</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>20031205</creationdate><title>The role of the Aspergillus niger furin-type protease gene in processing of fungal proproteins and fusion proteins : Evidence for alternative processing of recombinant (fusion-) proteins</title><author>Punt, P.J ; Drint-Kuijvenhoven, A ; Lokman, B.C ; Spencer, J.A ; Jeenes, D ; Archer, D.A ; van den Hondel, C.A.M.J.J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e324t-f5a6b8a89881f1151a3f63388851327b690dd7b09aa20cc6d6fa62e16d7e90433</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>aspergillopepsin</topic><topic>Aspergillus niger</topic><topic>Aspergillus niger - genetics</topic><topic>Aspergillus niger - metabolism</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Endopeptidases - genetics</topic><topic>Endopeptidases - metabolism</topic><topic>endoproteinase</topic><topic>Filamentous fungi</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fungal Proteins - biosynthesis</topic><topic>Fungal Proteins - genetics</topic><topic>furin</topic><topic>Furin - genetics</topic><topic>Furin - metabolism</topic><topic>Fusion-proteins</topic><topic>Gene Expression Regulation, Enzymologic - physiology</topic><topic>Gene Expression Regulation, Fungal - physiology</topic><topic>kexB gene</topic><topic>Protease</topic><topic>Protein processing</topic><topic>Protein Processing, Post-Translational - physiology</topic><topic>Protein secretion</topic><topic>Recombinant Fusion Proteins - biosynthesis</topic><topic>Recombinant Fusion Proteins - genetics</topic><topic>Secretion carrier</topic><topic>Transcriptional Activation - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Punt, P.J</creatorcontrib><creatorcontrib>Drint-Kuijvenhoven, A</creatorcontrib><creatorcontrib>Lokman, B.C</creatorcontrib><creatorcontrib>Spencer, J.A</creatorcontrib><creatorcontrib>Jeenes, D</creatorcontrib><creatorcontrib>Archer, D.A</creatorcontrib><creatorcontrib>van den Hondel, C.A.M.J.J</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Punt, P.J</au><au>Drint-Kuijvenhoven, A</au><au>Lokman, B.C</au><au>Spencer, J.A</au><au>Jeenes, D</au><au>Archer, D.A</au><au>van den Hondel, C.A.M.J.J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The role of the Aspergillus niger furin-type protease gene in processing of fungal proproteins and fusion proteins : Evidence for alternative processing of recombinant (fusion-) proteins</atitle><jtitle>Journal of biotechnology</jtitle><addtitle>J Biotechnol</addtitle><date>2003-12-05</date><risdate>2003</risdate><volume>106</volume><issue>1</issue><spage>23</spage><epage>32</epage><pages>23-32</pages><issn>0168-1656</issn><eissn>1873-4863</eissn><coden>JBITD4</coden><abstract>We have characterized growth and protein processing characteristics of
Aspergillus niger strains carrying a disrupted allele of the previously cloned and characterized
kexB gene [Appl. Environ. Microbiol. 66 (2000) 363] encoding a furin-type endoprotease. Deletion of the single-copy gene confirms it to be non-essential but disruptant strains exhibit a morphologically distinct phenotype characterized by hyperbranching. Processing of homologous pro-proteins and fusion proteins comprised of a heterologous protein fused down-stream of glucoamylase and separated at the fusion junction by an endoproteolytic cleavage site was compared in wildtype and mutant strains of
A. niger. We show that maturation of the native glucoamylase requires KexB, whereas maturation of aspergillopepsin does not. The processing of fusion proteins carrying Lys–Arg requires KexB, although alternative endoproteases are capable of cleaving protein fusions at sites adjacent to Lys–Arg.</abstract><cop>Lausanne</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>14636707</pmid><doi>10.1016/j.jbiotec.2003.09.005</doi><tpages>10</tpages></addata></record> |
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| subjects | aspergillopepsin Aspergillus niger Aspergillus niger - genetics Aspergillus niger - metabolism Biological and medical sciences Biotechnology Endopeptidases - genetics Endopeptidases - metabolism endoproteinase Filamentous fungi Fundamental and applied biological sciences. Psychology Fungal Proteins - biosynthesis Fungal Proteins - genetics furin Furin - genetics Furin - metabolism Fusion-proteins Gene Expression Regulation, Enzymologic - physiology Gene Expression Regulation, Fungal - physiology kexB gene Protease Protein processing Protein Processing, Post-Translational - physiology Protein secretion Recombinant Fusion Proteins - biosynthesis Recombinant Fusion Proteins - genetics Secretion carrier Transcriptional Activation - physiology |
| title | The role of the Aspergillus niger furin-type protease gene in processing of fungal proproteins and fusion proteins : Evidence for alternative processing of recombinant (fusion-) proteins |
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