Characterization of the myostatin gene in the gilthead seabream (Sparus aurata): sequence, genomic structure, and expression pattern
We report on the sequence and expression analysis of the myostatin gene (MSTN) in the gilthead seabream Sparus aurata. A 2189-bp transcript was isolated, encoding an open reading frame (385 amino acids) that showed 74% to 60% protein similarity with other vertebrate myostatins. Phylogenetic analysis...
Gespeichert in:
| Veröffentlicht in: | Marine biotechnology (New York, N.Y.) N.Y.), 2001-05, Vol.3 (3), p.224-230 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 230 |
|---|---|
| container_issue | 3 |
| container_start_page | 224 |
| container_title | Marine biotechnology (New York, N.Y.) |
| container_volume | 3 |
| creator | Maccatrozzo, L Bargelloni, L Radaelli, G Mascarello, F Patarnello, T |
| description | We report on the sequence and expression analysis of the myostatin gene (MSTN) in the gilthead seabream Sparus aurata. A 2189-bp transcript was isolated, encoding an open reading frame (385 amino acids) that showed 74% to 60% protein similarity with other vertebrate myostatins. Phylogenetic analysis of MSTN and other related genes confirmed the evolutionary relationships of the isolated sequence. The complete sequences of two introns were also determined. Intron-exon boundaries were conserved when compared with those of mammalian MSTN genes, whereas intron size was smaller. Reverse transcriptase polymerase chain reaction on total RNA extracted from different tissues and developmental stages revealed MSTN expression in the skeletal muscle, but also in other tissues. The observed expression profile differed from that in mammals, suggesting possible additional functions of myostatin in the teleost fish. |
| doi_str_mv | 10.1007/s101260000064 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_71386303</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1938923707</sourcerecordid><originalsourceid>FETCH-LOGICAL-c443t-1599a1c0469361faabb282080c1f527494bcd63b93403c533a99c05e506192723</originalsourceid><addsrcrecordid>eNqFkctLHTEUxoO01Oey2xIoSAVHc3IymYk7uagtCC7U9XAmN3MdufNokgF13T-8Ge6l0m6azRe-_HIefIx9BnEGQhTnAQRILeaj1Q7bA4U6kxL1hz93We6y_RCeE1IUKD6xXVBGA-Zmj_1aPJEnG51v3yi2Q8-Hhscnx7vXIcTk9HzleseTzu6qXSehJQ-Oau-o49_uR_JT4DR5inRykV5-Tq637nT-OHSt5SH6ycbJJ4v6JXcvo3chzL1Giqlzf8g-NrQO7mirB-zx-uph8T27vbv5sbi8zaxSGDPIjSGwQmmDGhqiupalFKWw0OSyUEbVdqmxNqgE2hyRjLEid7nQYGQh8YAdb-qOfkhDhlh1bbBuvabeDVOoCsBSo8D_glCCFrmewa__gM_D5Pu0RAUGSyOxEEWisg1l_RCCd001-rYj_1qBqOYUq79STPyXbdWp7tzynd7Ghr8B8sOWtw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1938923707</pqid></control><display><type>article</type><title>Characterization of the myostatin gene in the gilthead seabream (Sparus aurata): sequence, genomic structure, and expression pattern</title><source>SpringerLink Journals - AutoHoldings</source><creator>Maccatrozzo, L ; Bargelloni, L ; Radaelli, G ; Mascarello, F ; Patarnello, T</creator><creatorcontrib>Maccatrozzo, L ; Bargelloni, L ; Radaelli, G ; Mascarello, F ; Patarnello, T</creatorcontrib><description>We report on the sequence and expression analysis of the myostatin gene (MSTN) in the gilthead seabream Sparus aurata. A 2189-bp transcript was isolated, encoding an open reading frame (385 amino acids) that showed 74% to 60% protein similarity with other vertebrate myostatins. Phylogenetic analysis of MSTN and other related genes confirmed the evolutionary relationships of the isolated sequence. The complete sequences of two introns were also determined. Intron-exon boundaries were conserved when compared with those of mammalian MSTN genes, whereas intron size was smaller. Reverse transcriptase polymerase chain reaction on total RNA extracted from different tissues and developmental stages revealed MSTN expression in the skeletal muscle, but also in other tissues. The observed expression profile differed from that in mammals, suggesting possible additional functions of myostatin in the teleost fish.</description><identifier>ISSN: 1436-2228</identifier><identifier>EISSN: 1436-2236</identifier><identifier>DOI: 10.1007/s101260000064</identifier><identifier>PMID: 14961359</identifier><language>eng</language><publisher>United States: Springer Nature B.V</publisher><subject>Amino acids ; Biological evolution ; Developmental stages ; Fish ; Gene expression ; Gene sequencing ; Genes ; Introns ; Mammals ; Marine ; MSTN gene ; Myostatin ; Nucleic acids ; Nucleotide sequence ; PCR ; Phylogeny ; Polymerase chain reaction ; Proteins ; Ribonucleic acid ; RNA ; RNA-directed DNA polymerase ; Sequencing ; Skeletal muscle ; Sparus aurata ; Tissue ; Transcription</subject><ispartof>Marine biotechnology (New York, N.Y.), 2001-05, Vol.3 (3), p.224-230</ispartof><rights>Springer-Verlag New York Inc. 2001</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c443t-1599a1c0469361faabb282080c1f527494bcd63b93403c533a99c05e506192723</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14961359$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Maccatrozzo, L</creatorcontrib><creatorcontrib>Bargelloni, L</creatorcontrib><creatorcontrib>Radaelli, G</creatorcontrib><creatorcontrib>Mascarello, F</creatorcontrib><creatorcontrib>Patarnello, T</creatorcontrib><title>Characterization of the myostatin gene in the gilthead seabream (Sparus aurata): sequence, genomic structure, and expression pattern</title><title>Marine biotechnology (New York, N.Y.)</title><addtitle>Mar Biotechnol (NY)</addtitle><description>We report on the sequence and expression analysis of the myostatin gene (MSTN) in the gilthead seabream Sparus aurata. A 2189-bp transcript was isolated, encoding an open reading frame (385 amino acids) that showed 74% to 60% protein similarity with other vertebrate myostatins. Phylogenetic analysis of MSTN and other related genes confirmed the evolutionary relationships of the isolated sequence. The complete sequences of two introns were also determined. Intron-exon boundaries were conserved when compared with those of mammalian MSTN genes, whereas intron size was smaller. Reverse transcriptase polymerase chain reaction on total RNA extracted from different tissues and developmental stages revealed MSTN expression in the skeletal muscle, but also in other tissues. The observed expression profile differed from that in mammals, suggesting possible additional functions of myostatin in the teleost fish.</description><subject>Amino acids</subject><subject>Biological evolution</subject><subject>Developmental stages</subject><subject>Fish</subject><subject>Gene expression</subject><subject>Gene sequencing</subject><subject>Genes</subject><subject>Introns</subject><subject>Mammals</subject><subject>Marine</subject><subject>MSTN gene</subject><subject>Myostatin</subject><subject>Nucleic acids</subject><subject>Nucleotide sequence</subject><subject>PCR</subject><subject>Phylogeny</subject><subject>Polymerase chain reaction</subject><subject>Proteins</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA-directed DNA polymerase</subject><subject>Sequencing</subject><subject>Skeletal muscle</subject><subject>Sparus aurata</subject><subject>Tissue</subject><subject>Transcription</subject><issn>1436-2228</issn><issn>1436-2236</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNqFkctLHTEUxoO01Oey2xIoSAVHc3IymYk7uagtCC7U9XAmN3MdufNokgF13T-8Ge6l0m6azRe-_HIefIx9BnEGQhTnAQRILeaj1Q7bA4U6kxL1hz93We6y_RCeE1IUKD6xXVBGA-Zmj_1aPJEnG51v3yi2Q8-Hhscnx7vXIcTk9HzleseTzu6qXSehJQ-Oau-o49_uR_JT4DR5inRykV5-Tq637nT-OHSt5SH6ycbJJ4v6JXcvo3chzL1Giqlzf8g-NrQO7mirB-zx-uph8T27vbv5sbi8zaxSGDPIjSGwQmmDGhqiupalFKWw0OSyUEbVdqmxNqgE2hyRjLEid7nQYGQh8YAdb-qOfkhDhlh1bbBuvabeDVOoCsBSo8D_glCCFrmewa__gM_D5Pu0RAUGSyOxEEWisg1l_RCCd001-rYj_1qBqOYUq79STPyXbdWp7tzynd7Ghr8B8sOWtw</recordid><startdate>20010501</startdate><enddate>20010501</enddate><creator>Maccatrozzo, L</creator><creator>Bargelloni, L</creator><creator>Radaelli, G</creator><creator>Mascarello, F</creator><creator>Patarnello, T</creator><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QO</scope><scope>7TN</scope><scope>7U9</scope><scope>7WY</scope><scope>7WZ</scope><scope>7X7</scope><scope>7XB</scope><scope>87Z</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8FL</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BEZIV</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FR3</scope><scope>FRNLG</scope><scope>FYUFA</scope><scope>F~G</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>H95</scope><scope>H98</scope><scope>H99</scope><scope>HCIFZ</scope><scope>K60</scope><scope>K6~</scope><scope>K9.</scope><scope>L.-</scope><scope>L.F</scope><scope>L.G</scope><scope>L6V</scope><scope>LK8</scope><scope>M0C</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>P64</scope><scope>PCBAR</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PJZUB</scope><scope>PKEHL</scope><scope>PPXIY</scope><scope>PQBIZ</scope><scope>PQBZA</scope><scope>PQEST</scope><scope>PQGLB</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20010501</creationdate><title>Characterization of the myostatin gene in the gilthead seabream (Sparus aurata): sequence, genomic structure, and expression pattern</title><author>Maccatrozzo, L ; Bargelloni, L ; Radaelli, G ; Mascarello, F ; Patarnello, T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c443t-1599a1c0469361faabb282080c1f527494bcd63b93403c533a99c05e506192723</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Amino acids</topic><topic>Biological evolution</topic><topic>Developmental stages</topic><topic>Fish</topic><topic>Gene expression</topic><topic>Gene sequencing</topic><topic>Genes</topic><topic>Introns</topic><topic>Mammals</topic><topic>Marine</topic><topic>MSTN gene</topic><topic>Myostatin</topic><topic>Nucleic acids</topic><topic>Nucleotide sequence</topic><topic>PCR</topic><topic>Phylogeny</topic><topic>Polymerase chain reaction</topic><topic>Proteins</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA-directed DNA polymerase</topic><topic>Sequencing</topic><topic>Skeletal muscle</topic><topic>Sparus aurata</topic><topic>Tissue</topic><topic>Transcription</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Maccatrozzo, L</creatorcontrib><creatorcontrib>Bargelloni, L</creatorcontrib><creatorcontrib>Radaelli, G</creatorcontrib><creatorcontrib>Mascarello, F</creatorcontrib><creatorcontrib>Patarnello, T</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Oceanic Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>ABI/INFORM Collection</collection><collection>ABI/INFORM Global (PDF only)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ABI/INFORM Global (Alumni Edition)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ABI/INFORM Collection (Alumni Edition)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Business Premium Collection</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Earth, Atmospheric & Aquatic Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Business Premium Collection (Alumni)</collection><collection>Health Research Premium Collection</collection><collection>ABI/INFORM Global (Corporate)</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Business Collection (Alumni Edition)</collection><collection>ProQuest Business Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ABI/INFORM Professional Advanced</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>ABI/INFORM Global</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Earth, Atmospheric & Aquatic Science Database</collection><collection>ProQuest Central (New)</collection><collection>ProQuest One Academic (New)</collection><collection>ProQuest Health & Medical Research Collection</collection><collection>ProQuest One Academic Middle East (New)</collection><collection>ProQuest One Health & Nursing</collection><collection>ProQuest One Business</collection><collection>ProQuest One Business (Alumni)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Applied & Life Sciences</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Marine biotechnology (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Maccatrozzo, L</au><au>Bargelloni, L</au><au>Radaelli, G</au><au>Mascarello, F</au><au>Patarnello, T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of the myostatin gene in the gilthead seabream (Sparus aurata): sequence, genomic structure, and expression pattern</atitle><jtitle>Marine biotechnology (New York, N.Y.)</jtitle><addtitle>Mar Biotechnol (NY)</addtitle><date>2001-05-01</date><risdate>2001</risdate><volume>3</volume><issue>3</issue><spage>224</spage><epage>230</epage><pages>224-230</pages><issn>1436-2228</issn><eissn>1436-2236</eissn><abstract>We report on the sequence and expression analysis of the myostatin gene (MSTN) in the gilthead seabream Sparus aurata. A 2189-bp transcript was isolated, encoding an open reading frame (385 amino acids) that showed 74% to 60% protein similarity with other vertebrate myostatins. Phylogenetic analysis of MSTN and other related genes confirmed the evolutionary relationships of the isolated sequence. The complete sequences of two introns were also determined. Intron-exon boundaries were conserved when compared with those of mammalian MSTN genes, whereas intron size was smaller. Reverse transcriptase polymerase chain reaction on total RNA extracted from different tissues and developmental stages revealed MSTN expression in the skeletal muscle, but also in other tissues. The observed expression profile differed from that in mammals, suggesting possible additional functions of myostatin in the teleost fish.</abstract><cop>United States</cop><pub>Springer Nature B.V</pub><pmid>14961359</pmid><doi>10.1007/s101260000064</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1436-2228 |
| ispartof | Marine biotechnology (New York, N.Y.), 2001-05, Vol.3 (3), p.224-230 |
| issn | 1436-2228 1436-2236 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_71386303 |
| source | SpringerLink Journals - AutoHoldings |
| subjects | Amino acids Biological evolution Developmental stages Fish Gene expression Gene sequencing Genes Introns Mammals Marine MSTN gene Myostatin Nucleic acids Nucleotide sequence PCR Phylogeny Polymerase chain reaction Proteins Ribonucleic acid RNA RNA-directed DNA polymerase Sequencing Skeletal muscle Sparus aurata Tissue Transcription |
| title | Characterization of the myostatin gene in the gilthead seabream (Sparus aurata): sequence, genomic structure, and expression pattern |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-21T21%3A26%3A00IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Characterization%20of%20the%20myostatin%20gene%20in%20the%20gilthead%20seabream%20(Sparus%20aurata):%20sequence,%20genomic%20structure,%20and%20expression%20pattern&rft.jtitle=Marine%20biotechnology%20(New%20York,%20N.Y.)&rft.au=Maccatrozzo,%20L&rft.date=2001-05-01&rft.volume=3&rft.issue=3&rft.spage=224&rft.epage=230&rft.pages=224-230&rft.issn=1436-2228&rft.eissn=1436-2236&rft_id=info:doi/10.1007/s101260000064&rft_dat=%3Cproquest_cross%3E1938923707%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1938923707&rft_id=info:pmid/14961359&rfr_iscdi=true |