Oligonucleotide Arrays for Expression Monitoring

This unit provides protocols for the amplification and labeling of mRNA (and the necessary controls) for hybridization to oligonucleotide arrays. It also describes methods for processing and normalizing the raw gene expression data in preparation for clustering and further analysis.

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Bibliographische Detailangaben
Veröffentlicht in:	Current Protocols in Human Genetics 2001-05, Vol.25 (1), p.11.2.1-11.2.16
Hauptverfasser:	Byrne, Michael C., Whitley, Maryann Z., Follettie, Maximillian T.
Format:	Artikel
Sprache:	eng
Schlagworte:	DNA, Complementary - genetics DNA, Complementary - isolation & purification Gene Expression Profiling - methods Genetics, Medical Humans Nucleic Acid Amplification Techniques Oligonucleotide Array Sequence Analysis - methods RNA, Messenger - genetics
Online-Zugang:	Volltext
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Beschreibung
Zusammenfassung:	This unit provides protocols for the amplification and labeling of mRNA (and the necessary controls) for hybridization to oligonucleotide arrays. It also describes methods for processing and normalizing the raw gene expression data in preparation for clustering and further analysis.
ISSN:	1934-8266 1934-8258
DOI:	10.1002/0471142905.hg1102s25