Identification of CD4 and transferrin receptor antibodies by CXCR4 antibody‐guided Pathfinder selection

To generate human antibodies against CXCR4, a seven‐transmembrane chemokine receptor and a principal coreceptor for HIV‐1, several rounds of Pathfinder and Step‐back selection from a large phage display antibody library were performed on Jurkat cells. A mAb against CXCR4 or biotinyated phage antibod...

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Veröffentlicht in:	European journal of biochemistry 2003-11, Vol.270 (22), p.4497-4506
Hauptverfasser:	Sui, Jianhua, Bai, Jirong, St. Clair Tallarico, Aimee, Xu, Chen, Marasco, Wayne A
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Antibodies - chemistry Antibodies - immunology Antibodies - isolation & purification Antibodies, Monoclonal - immunology Antibody Specificity CD4 Antigens - immunology Cell Line, Tumor CXCR4 Enzyme-Linked Immunosorbent Assay Flow Cytometry HeLa Cells Humans Immunoglobulin Heavy Chains - chemistry Immunoglobulin Heavy Chains - immunology Immunoglobulin Heavy Chains - isolation & purification Immunoglobulin Light Chains - chemistry Immunoglobulin Light Chains - immunology Immunoglobulin Light Chains - isolation & purification Immunoglobulin Variable Region Jurkat Cells Molecular Sequence Data Pathfinder selection Peptide Library phage display Receptors, CXCR4 - immunology Receptors, Transferrin - immunology scFv transferrin receptor
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